Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,754,950 T→A 100% I367F (ATT→TTT)  sufD ← component of SufBCD Fe‑S cluster assembly scaffold

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,754,9500TA100.0% 582.2 / NA 186I367F (ATT→TTT) sufDcomponent of SufBCD Fe‑S cluster assembly scaffold
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (104/82);  total (104/82)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 186 total aligned reads displayed.

CGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAATACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGA  >  CP009273/1754806‑1755093
                                                                                                                                                |                                                                                                                                               
cGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACg                                                                                                                                              <  2:626887/148‑1 (MQ=255)
        gCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCg                                                                                                                                      <  2:2572418/148‑1 (MQ=255)
        gCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCg                                                                                                                                      <  1:163037/148‑1 (MQ=255)
        gCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCg                                                                                                                                      <  1:1858827/148‑1 (MQ=255)
         ccAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGt                                                                                                                                     <  2:65686/148‑1 (MQ=255)
          cAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTc                                                                                                                                    >  1:1291933/1‑148 (MQ=255)
          cAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTc                                                                                                                                    <  1:3558177/148‑1 (MQ=255)
           aGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTcg                                                                                                                                   <  1:3407263/148‑1 (MQ=255)
              aCCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCgcgc                                                                                                                                >  2:1423475/1‑148 (MQ=255)
               ccTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGcc                                                                                                                               >  2:427595/1‑148 (MQ=255)
                ctgctgTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCg                                                                                                                              <  1:3348083/148‑1 (MQ=255)
                   ctgTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCGGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTgg                                                                                                                           <  2:2149558/148‑1 (MQ=255)
                    tgTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGc                                                                                                                          >  1:2678224/1‑148 (MQ=255)
                      tttAAGCCCCTCATCACTCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTg                                                                                                                        >  1:2895070/1‑148 (MQ=255)
                      tttAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTg                                                                                                                        >  2:2392125/1‑148 (MQ=255)
                       ttAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGc                                                                                                                       >  2:987567/1‑148 (MQ=255)
                        tAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCa                                                                                                                      <  1:2570637/148‑1 (MQ=255)
                          aGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGCGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCAtt                                                                                                                    <  1:1542949/148‑1 (MQ=255)
                            ccccTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTc                                                                                                                  >  2:2051772/1‑148 (MQ=255)
                             cccTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTca                                                                                                                 >  2:1614078/1‑148 (MQ=255)
                             cccTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTca                                                                                                                 >  1:1082937/1‑148 (MQ=255)
                              ccTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTcac                                                                                                                >  1:1048402/1‑148 (MQ=255)
                              ccTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTcac                                                                                                                >  1:1048384/1‑148 (MQ=255)
                                tcatcaCGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCAcat                                                                                                              <  1:2635296/148‑1 (MQ=255)
                                  atcaCGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCAcatca                                                                                                            <  2:1128382/148‑1 (MQ=255)
                                  atcaCGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCAcatca                                                                                                            <  2:348851/148‑1 (MQ=255)
                                   tcaCGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCAcatcat                                                                                                           >  1:655000/1‑148 (MQ=255)
                                    caCGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACatcatc                                                                                                          >  2:1096029/1‑148 (MQ=255)
                                    caCGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACatcatc                                                                                                          >  2:2251277/1‑148 (MQ=255)
                                    caCGCAGTGCATCAGTCAGTAATGCAGCGAAGGAGTAAATGAACATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGACGCGCCGAGGCTGCAGTTCACatcatc                                                                                                          >  2:2250646/1‑148 (MQ=255)
                                     aCGCAGTGCTTCCGTCAGTTCGTCAGCGAAGGCGTAAATGATCATCTGCTGGTCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCt                                                                                                         >  2:12004/1‑148 (MQ=255)
                                      cGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTg                                                                                                        >  2:909959/1‑148 (MQ=255)
                                       gCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGCGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGc                                                                                                       <  1:1208335/148‑1 (MQ=255)
                                         aGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCAt                                                                                                     <  2:3018457/148‑1 (MQ=255)
                                                ccGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTc                                                                                              <  2:2968821/148‑1 (MQ=255)
                                                   tCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAg                                                                                           >  2:1927442/1‑148 (MQ=255)
                                                    cAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGc                                                                                          >  2:304145/1‑148 (MQ=255)
                                                    cAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGc                                                                                          <  2:1291933/148‑1 (MQ=255)
                                                         cGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCgg                                                                                     <  1:3323863/148‑1 (MQ=255)
                                                          ggCAGTGATGGCGTAAATGATCATCTGTTGGGATTCCTGCTGTTTGATCCCGCTCGAGCGCAGATATAATATCTGTTATTCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGt                                                                                    <  1:1847031/148‑1 (MQ=255)
                                                          ggCAGCGAAGGCGTAATTGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGt                                                                                    <  1:142681/148‑1 (MQ=255)
                                                           gCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGtt                                                                                   <  1:208618/148‑1 (MQ=255)
                                                            cAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGttt                                                                                  >  2:2528195/1‑148 (MQ=255)
                                                            cAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGttt                                                                                  >  1:566574/1‑148 (MQ=255)
                                                            cAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGttt                                                                                  >  1:566663/1‑148 (MQ=255)
                                                               cGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGt                                                                               <  1:909959/148‑1 (MQ=255)
                                                               cGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGt                                                                               >  1:2198170/1‑148 (MQ=255)
                                                               cGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGt                                                                               <  1:2051772/148‑1 (MQ=255)
                                                                  aGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATc                                                                            >  2:2282396/1‑148 (MQ=255)
                                                                   ggCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATcc                                                                           <  1:315196/148‑1 (MQ=255)
                                                                    gCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCa                                                                          >  1:532561/1‑148 (MQ=255)
                                                                       tAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACtt                                                                       <  1:1344081/148‑1 (MQ=255)
                                                                       tAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACtt                                                                       <  1:2924489/148‑1 (MQ=255)
                                                                          aTGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCg                                                                    >  2:1959977/1‑148 (MQ=255)
                                                                           tGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGc                                                                   >  2:828228/1‑148 (MQ=255)
                                                                           tGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGc                                                                   >  1:2013199/1‑148 (MQ=255)
                                                                           tGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGc                                                                   <  2:1527413/148‑1 (MQ=255)
                                                                              tcatcTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAg                                                                >  1:2163451/1‑148 (MQ=255)
                                                                                 tcTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGttt                                                             >  1:1680699/1‑148 (MQ=255)
                                                                                  ctgctgGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTg                                                            >  1:1411291/1‑148 (MQ=255)
                                                                                       gggCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCAt                                                       >  2:3181329/1‑148 (MQ=255)
                                                                                        ggCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATc                                                      >  2:534831/1‑148 (MQ=255)
                                                                                        ggCATCCTGCTGATAGAACCCGCGCGTGCGCACATAGAATATGTGTTCATAATCAAAACGCCCCACCGTCGCGCCGTGGCTGAATTTCACATCATCTGCATAGATTTACAGCTGCGGTTTCGTATCCACTTCCTCCATGTAGCCCATc                                                      >  2:534554/1‑148 (MQ=255)
                                                                                              ctgctgATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGa                                                <  2:1653455/148‑1 (MQ=255)
                                                                                              ctgctgATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGa                                                <  2:1048402/148‑1 (MQ=255)
                                                                                              ctgctgATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGa                                                <  1:2392125/148‑1 (MQ=255)
                                                                                               tgctgATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGAt                                               <  1:177719/148‑1 (MQ=255)
                                                                                                gctgATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGAtt                                              <  2:1683690/148‑1 (MQ=255)
                                                                                                 ctgATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGAttg                                             <  1:2366389/148‑1 (MQ=255)
                                                                                                   gattgatCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGAttgtt                                           >  2:2655005/1‑148 (MQ=255)
                                                                                                      tgatCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGAttgttgtt                                        >  2:2545407/1‑148 (MQ=255)
                                                                                                         tCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGt                                     <  2:1932000/148‑1 (MQ=255)
                                                                                                          cccGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTc                                    >  2:2348340/1‑148 (MQ=255)
                                                                                                           ccGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCa                                   >  1:1276861/1‑148 (MQ=255)
                                                                                                           ccGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCa                                   >  1:1919589/1‑148 (MQ=255)
                                                                                                            cgcgcgAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCAt                                  <  2:2732676/148‑1 (MQ=255)
                                                                                                             gcgcgAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCCGTTTGCCCATCAGCAGATTGTTGTTGGTCATc                                 <  1:992833/148‑1 (MQ=255)
                                                                                                                  aGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGAcc                            <  2:1187572/148‑1 (MQ=255)
                                                                                                                   gcgcAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCa                           >  1:3316667/1‑148 (MQ=255)
                                                                                                                    cgcAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCAt                          >  2:1908048/1‑148 (MQ=255)
                                                                                                                    cgcAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCAt                          <  1:427595/148‑1 (MQ=255)
                                                                                                                    cgcAGATAGAATATCTGGTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCAt                          >  1:2135220/1‑148 (MQ=255)
                                                                                                                       aggtAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCg                       <  2:1482752/145‑1 (MQ=255)
                                                                                                                       aGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCg                       <  2:2723797/148‑1 (MQ=255)
                                                                                                                         aTAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGtt                     >  1:1521407/1‑148 (MQ=255)
                                                                                                                         aTAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGATGGTCATCTGACCATCCGtt                     >  2:1839550/1‑148 (MQ=255)
                                                                                                                          tAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGttt                    <  1:303031/148‑1 (MQ=255)
                                                                                                                           aGAATATCTGTTCATCATCACAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGtttt                   <  2:3341943/148‑1 (MQ=255)
                                                                                                                             aaTATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGa                 <  1:2772352/148‑1 (MQ=255)
                                                                                                                             aaTATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGa                 >  1:272236/1‑148 (MQ=255)
                                                                                                                              atatCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGAt                >  2:798254/1‑148 (MQ=255)
                                                                                                                               tatCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATg               >  2:2365069/1‑148 (MQ=255)
                                                                                                                               tatCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATg               >  2:1172846/1‑148 (MQ=255)
                                                                                                                               tatCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATg               >  2:2370550/1‑148 (MQ=255)
                                                                                                                               tatCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATg               >  2:3475017/1‑148 (MQ=255)
                                                                                                                                  cTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCg            >  1:3525892/1‑148 (MQ=255)
                                                                                                                                  cTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCg            >  1:2102533/1‑148 (MQ=255)
                                                                                                                                  cTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCg            >  2:150730/1‑148 (MQ=255)
                                                                                                                                         tcatcaAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTgcg     >  2:2018160/1‑148 (MQ=255)
                                                                                                                                            tcaAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGa  >  1:654401/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                               
CGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAATACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGA  >  CP009273/1754806‑1755093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑