Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,796,101 C→A 100% D243Y (GAC→TAC)  thrS ← threonyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,796,1010CA95.4% 588.4 / 15.4 208D243Y (GAC→TAC) thrSthreonyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (6/4);  new base A (104/94);  total (110/98)
Fisher's exact test for biased strand distribution p-value = 7.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 212 total aligned reads displayed.

TGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGAAATGA  >  CP009273/1795956‑1796246
                                                                                                                                                 |                                                                                                                                                 
tGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTagc                                                                                                                                                 >  2:3327158/1‑148 (MQ=255)
tGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTagc                                                                                                                                                 >  2:1892619/1‑148 (MQ=255)
 gATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTagcg                                                                                                                                                >  1:1051799/1‑148 (MQ=255)
  aTACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGt                                                                                                                                               >  2:731914/1‑148 (MQ=255)
  aTACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGt                                                                                                                                               <  1:1159721/148‑1 (MQ=255)
  aTACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGGAGCGt                                                                                                                                               >  1:1179499/1‑148 (MQ=255)
      tGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTcg                                                                                                                                           >  2:353556/1‑148 (MQ=255)
        gTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTcgcg                                                                                                                                         <  2:1134614/148‑1 (MQ=255)
          aCTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGc                                                                                                                                       <  2:640694/148‑1 (MQ=255)
                tCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCttcttc                                                                                                                                 >  2:2651338/1‑148 (MQ=255)
                 cAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTcc                                                                                                                                <  2:1745557/148‑1 (MQ=255)
                     ttAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGgc                                                                                                                            >  1:1342057/1‑148 (MQ=255)
                     ttAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGgc                                                                                                                            >  2:1254490/1‑148 (MQ=255)
                      tAGAACGAACAAACACGTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGgcg                                                                                                                           <  1:3009383/148‑1 (MQ=255)
                       aGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGgcgc                                                                                                                          <  2:640843/148‑1 (MQ=255)
                         aacgaacAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTg                                                                                                                        >  1:1079841/1‑148 (MQ=255)
                         aacgaacAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTg                                                                                                                        >  2:3168942/1‑148 (MQ=255)
                         aacgaacAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTAAAGGTCGAGCGGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTg                                                                                                                        >  1:2159276/1‑148 (MQ=255)
                          acgaacAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGc                                                                                                                       >  1:848676/1‑148 (MQ=255)
                          acgaacAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGc                                                                                                                       >  1:848659/1‑148 (MQ=255)
                           cgaacAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCa                                                                                                                      >  1:2455537/1‑148 (MQ=255)
                              acaaacaCTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGt                                                                                                                   <  1:1936907/148‑1 (MQ=255)
                               caaacaCTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTa                                                                                                                  <  2:2931502/148‑1 (MQ=255)
                               caaacaCTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTa                                                                                                                  <  1:2643516/148‑1 (MQ=255)
                                  acacTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGc                                                                                                               >  1:415179/1‑148 (MQ=255)
                                     cTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTAAGGTGGTAGCGTTTCGCGGCTTCTTCAAGGCGCTGCAGGTAAGCGtt                                                                                                            >  1:2371640/1‑148 (MQ=255)
                                      ttCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTAACGAGTTTACGGGGGTAGCGTTTAGCGGCGTCTTCCAGGCGCTGCCGGTAAGCGTTa                                                                                                           >  1:690967/1‑148 (MQ=255)
                                        ccAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAg                                                                                                         >  2:3261206/1‑148 (MQ=255)
                                        ccAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAg                                                                                                         <  2:1775348/148‑1 (MQ=255)
                                        ccAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAg                                                                                                         >  1:3215740/1‑148 (MQ=255)
                                        ccAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAg                                                                                                         >  1:3214808/1‑148 (MQ=255)
                                          aGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTg                                                                                                       <  2:1365675/148‑1 (MQ=255)
                                            ttCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCGGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGGTAAGTGCt                                                                                                     >  2:1614317/1‑148 (MQ=255)
                                             tCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATTGTACAGGTCGAGCTGTTTACCGATTTTAAGGTGGTAGCGTTTCGCGGCTTATTACAGGCGCTGCAGGTAAGCGGTTAGTGCtt                                                                                                    >  1:3438093/1‑148 (MQ=255)
                                               aCGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCtttt                                                                                                  >  2:3137610/1‑148 (MQ=255)
                                               aCGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCGTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCtttt                                                                                                  <  1:1411990/148‑1 (MQ=255)
                                                   aaGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGt                                                                                              <  1:305338/148‑1 (MQ=255)
                                                   aaGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGt                                                                                              <  2:2986532/148‑1 (MQ=255)
                                                    aGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCAAATGGTACAGGTCGAGCTGTTTAACGCATTTAAGGTGGTAGCGTTTAGCGGCGTCTTCCAGGCGCTGCAGGTAAGCGGTTAGTGCTTTTTTGTc                                                                                             >  1:2411345/1‑148 (MQ=255)
                                                      aTGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTg                                                                                           >  2:297700/1‑148 (MQ=255)
                                                              gCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTTCGGGGGTAGCGTTTAGCGGCGTGTTACAGGCGCGGCAGGTAAGCGGTAAGTGCTTTTTTTTCTGCCCACGcc                                                                                   >  1:97181/1‑148 (MQ=255)
                                                              gCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGcc                                                                                   >  2:2815099/1‑148 (MQ=255)
                                                              gCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGcc                                                                                   >  2:3073009/1‑148 (MQ=255)
                                                               ccGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGccg                                                                                  >  2:1958177/1‑148 (MQ=255)
                                                               ccGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGccg                                                                                  <  2:3285911/148‑1 (MQ=255)
                                                               ccGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGccg                                                                                  <  1:1598279/148‑1 (MQ=255)
                                                               ccGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGccg                                                                                  >  2:1242033/1‑148 (MQ=255)
                                                               ccGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTACTGCATAAGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGGTTAGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTTTCTGCCCccgccg                                                                                  >  1:773125/1‑148 (MQ=255)
                                                               ccGTCGTTGTGCCAGAATACCATACCCGGCGCGTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGGTTCGCGGCGTCTTCCAGGCGCTGCAGGGAAGCGTTAAGTGCTTTTTTTTCTGCCCACCccg                                                                                  >  1:3077631/1‑148 (MQ=255)
                                                                  tcgtTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGccgtac                                                                               >  2:1041448/1‑148 (MQ=255)
                                                                  tcgtTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGccgtac                                                                               >  2:1923240/1‑148 (MQ=255)
                                                                  tcgtTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGccgtac                                                                               >  2:125051/1‑148 (MQ=255)
                                                                      tgtgCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCGGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTACAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGccgtaccgta                                                                           >  2:1617162/1‑148 (MQ=255)
                                                                       gtgCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTaa                                                                          <  2:1203790/148‑1 (MQ=255)
                                                                       gtgCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTaa                                                                          <  2:3097999/148‑1 (MQ=255)
                                                                       gtgCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTaa                                                                          <  2:2975475/148‑1 (MQ=255)
                                                                        tgCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTaaa                                                                         >  2:832656/1‑148 (MQ=255)
                                                                        tgCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTaaa                                                                         <  1:3327412/148‑1 (MQ=255)
                                                                         gCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAAt                                                                        <  2:1678732/148‑1 (MQ=255)
                                                                             gAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGt                                                                    <  2:1793314/148‑1 (MQ=255)
                                                                               ataccataccCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTg                                                                  <  1:3549394/148‑1 (MQ=255)
                                                                                  ccataccCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTAGCGGCTTATTCCAGGCGCAGCAGGTAATCGTTAAGTGCTTTTTTTTCTGCCCACGCCGTACCGTAAATAAGTTGCaa                                                               >  1:532562/1‑148 (MQ=255)
                                                                                    ataccCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTAACGATTTTACGGTGGTAGCGTTTAGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTAACGTAAATAAGTTGCAACa                                                             >  2:300228/1‑148 (MQ=255)
                                                                                     taccCGGCGCTTCTTCCTGCAAATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACAt                                                            >  2:1290012/1‑148 (MQ=255)
                                                                                      accCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACAtt                                                           <  2:1079841/148‑1 (MQ=255)
                                                                                         cGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTttg                                                        <  2:1117059/148‑1 (MQ=255)
                                                                                              cttcttCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTttgttgtt                                                   >  1:951923/1‑148 (MQ=255)
                                                                                              cttcttCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTttgttgtt                                                   >  1:1284202/1‑148 (MQ=255)
                                                                                              cttcttCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTttgttgtt                                                   >  2:590754/1‑148 (MQ=255)
                                                                                                tcttcCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGc                                                 >  2:2281970/1‑148 (MQ=255)
                                                                                                 cttcCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCt                                                >  1:1655619/1‑148 (MQ=255)
                                                                                                 cttcCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCt                                                >  1:1946993/1‑148 (MQ=255)
                                                                                                  ttcCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTg                                               <  1:2543140/148‑1 (MQ=255)
                                                                                                   tcCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGt                                              <  1:2979302/148‑1 (MQ=255)
                                                                                                     cTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTcg                                            <  1:2530878/148‑1 (MQ=255)
                                                                                                      tGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTcgc                                           >  2:3431567/1‑148 (MQ=255)
                                                                                                           atGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTcgccacgc                                      >  2:2577496/1‑148 (MQ=255)
                                                                                                           atGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTcgccacgc                                      >  2:382295/1‑148 (MQ=255)
                                                                                                           atGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTcgccacgc                                      <  1:3116193/148‑1 (MQ=255)
                                                                                                             ggTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTcgccacgcca                                    <  1:2030712/148‑1 (MQ=255)
                                                                                                             ggTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTcgccacgcca                                    >  1:3129940/1‑148 (MQ=255)
                                                                                                              gTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAg                                   <  2:2455537/148‑1 (MQ=255)
                                                                                                               tACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGt                                  <  2:2720046/148‑1 (MQ=255)
                                                                                                               tACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGt                                  >  1:15610/1‑148 (MQ=255)
                                                                                                               tACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGt                                  >  1:2233323/1‑148 (MQ=255)
                                                                                                                    gTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGcc                             >  1:3310464/1‑148 (MQ=255)
                                                                                                                      cGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGcccc                           >  1:1515618/1‑148 (MQ=255)
                                                                                                                        aGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTg                         >  1:2732706/1‑148 (MQ=255)
                                                                                                                         gCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGc                        <  1:353556/148‑1 (MQ=255)
                                                                                                                          cTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGcc                       <  1:1242033/148‑1 (MQ=255)
                                                                                                                            gTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGt                     >  1:2991916/1‑148 (MQ=255)
                                                                                                                             tttACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGtg                    >  1:537263/1‑147 (MQ=255)
                                                                                                                              ttACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGttt                   <  2:566889/148‑1 (MQ=255)
                                                                                                                               tACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGtttt                  <  2:13437/148‑1 (MQ=255)
                                                                                                                                aCCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACTCCAGTAAGCCCCTGCCGTATTc                 >  2:2631542/1‑148 (MQ=255)
                                                                                                                                 ccGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTGTTCa                >  1:2875634/1‑148 (MQ=255)
                                                                                                                                     ttttACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAg            <  1:3168262/148‑1 (MQ=255)
                                                                                                                                      tttACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGt           <  1:1851804/148‑1 (MQ=255)
                                                                                                                                           ggtggtAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGaa      <  2:3469450/148‑1 (MQ=255)
                                                                                                                                              ggtAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTTAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGAAatg   >  2:2414741/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
TGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGAAATGA  >  CP009273/1795956‑1796246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑