Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,829,319 C→A 100% I85I (ATC→ATA)  ydjZ → TVP38/TMEM64 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,829,3190CA98.4% 603.6 / 0.7 188I85I (ATC→ATA) ydjZTVP38/TMEM64 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (94/91);  total (96/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 188 total aligned reads displayed.

CAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCT  >  CP009273/1829175‑1829465
                                                                                                                                                |                                                                                                                                                  
 aaTCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCt                                                                                                                                                <  2:2119797/148‑1 (MQ=255)
 aaTCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCt                                                                                                                                                <  2:2119851/148‑1 (MQ=255)
  aTCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCtt                                                                                                                                               <  2:1211384/148‑1 (MQ=255)
  aTCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCtt                                                                                                                                               <  2:197141/148‑1 (MQ=255)
   tCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCttt                                                                                                                                              <  2:2511246/148‑1 (MQ=255)
      cAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGcc                                                                                                                                           <  1:1007768/148‑1 (MQ=255)
        gCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCaa                                                                                                                                         >  1:3041222/1‑148 (MQ=255)
        gCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCaa                                                                                                                                         <  1:1282649/148‑1 (MQ=255)
         cGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAAt                                                                                                                                        >  1:1461175/1‑148 (MQ=255)
           ttGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGc                                                                                                                                      >  2:1066673/1‑148 (MQ=255)
             gcggcgTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGt                                                                                                                                    <  2:1564263/148‑1 (MQ=255)
             gcggcgTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGt                                                                                                                                    <  2:2898261/148‑1 (MQ=255)
                gcgTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTTCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGc                                                                                                                                 >  1:2477684/1‑148 (MQ=255)
                gcgTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGc                                                                                                                                 <  2:470060/148‑1 (MQ=255)
                gcgTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGc                                                                                                                                 <  1:637729/148‑1 (MQ=255)
                  gTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTg                                                                                                                               <  2:1368496/148‑1 (MQ=255)
                        cgccgTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGgc                                                                                                                         >  1:227494/1‑148 (MQ=255)
                           cgTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGcgcg                                                                                                                      <  1:1334581/148‑1 (MQ=255)
                             tGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCaa                                                                                                                                         >  1:2663852/1‑127 (MQ=255)
                             tGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGtt                                                                                                                    >  2:619236/1‑148 (MQ=255)
                                aCCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTg                                                                                                                 >  2:2672625/1‑148 (MQ=255)
                                aCCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTg                                                                                                                 <  2:2762104/148‑1 (MQ=255)
                                 ccAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTgg                                                                                                                >  2:2375866/1‑148 (MQ=255)
                                   aaCAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTgggg                                                                                                              >  1:1789266/1‑148 (MQ=255)
                                      aGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGggcgg                                                                                                           <  2:1180483/148‑1 (MQ=255)
                                       gggTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGggcggc                                                                                                          >  2:3355370/1‑148 (MQ=255)
                                       gggTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGggcggc                                                                                                          <  2:1145163/148‑1 (MQ=255)
                                       gggTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGggcggc                                                                                                          <  1:2886823/148‑1 (MQ=255)
                                          tataGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTg                                                                                                       >  2:871886/1‑148 (MQ=255)
                                          tataGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTg                                                                                                       <  1:1513645/148‑1 (MQ=255)
                                           ataGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGc                                                                                                      >  2:1217981/1‑148 (MQ=255)
                                           ataGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGc                                                                                                      >  2:2987668/1‑148 (MQ=255)
                                           ataGAACGATTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGGTGTGGGGCGGCTTGc                                                                                                      >  1:1503188/1‑148 (MQ=255)
                                             aGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTg                                                                                                    <  1:2540630/148‑1 (MQ=255)
                                              gAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGt                                                                                                   >  1:273158/1‑148 (MQ=255)
                                              gAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTGTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGt                                                                                                   >  1:273163/1‑148 (MQ=255)
                                                aCGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCg                                                                                                 <  2:2018549/148‑1 (MQ=255)
                                                 cGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGt                                                                                                >  2:3264812/1‑148 (MQ=255)
                                                    tttATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGa                                                                                             <  1:1015917/148‑1 (MQ=255)
                                                     ttATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGAc                                                                                            >  2:1816267/1‑148 (MQ=255)
                                                     ttATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGAc                                                                                            >  2:1817162/1‑148 (MQ=255)
                                                        ttCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAg                                                                                         <  2:1520241/148‑1 (MQ=255)
                                                         tCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTTGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGt                                                                                        >  1:91129/1‑148 (MQ=255)
                                                         tCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTGTTTGATAACCTTTGCCAAGGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGt                                                                                        >  2:3101199/1‑148 (MQ=255)
                                                          cAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGAcccggt                                                                                       >  1:634250/1‑144 (MQ=255)
                                                          cAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGGTTGGCGCGGTCTGGGGCGGCTTGCGGTCGTGGACCAGtt                                                                                       >  1:634267/1‑148 (MQ=255)
                                                            gTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCg                                                                                     >  1:194404/1‑148 (MQ=255)
                                                            gTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCg                                                                                     >  1:2339040/1‑148 (MQ=255)
                                                            gTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCg                                                                                     <  1:1194146/148‑1 (MQ=255)
                                                             tCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGa                                                                                    >  2:3194151/1‑148 (MQ=255)
                                                              cTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGAt                                                                                   <  1:1218315/148‑1 (MQ=255)
                                                                 aCGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTGTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGc                                                                                >  2:2972513/1‑148 (MQ=255)
                                                                     cgcACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCcggc                                                                            <  1:978588/148‑1 (MQ=255)
                                                                        aCTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCcggcgcg                                                                         >  2:233266/1‑148 (MQ=255)
                                                                        aCTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCcggcgcg                                                                         >  2:2027938/1‑148 (MQ=255)
                                                                          tGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCcggcgcggc                                                                       >  2:3317846/1‑148 (MQ=255)
                                                                           ggcggcAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCcggcgcggcg                                                                      <  2:3438428/148‑1 (MQ=255)
                                                                                cAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCtgtg                                                                 <  1:3238374/148‑1 (MQ=255)
                                                                                cAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCtgtg                                                                 >  1:2172577/1‑148 (MQ=255)
                                                                                 aGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGc                                                                <  2:1940214/148‑1 (MQ=255)
                                                                                   ttGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCtt                                                              >  2:1719135/1‑148 (MQ=255)
                                                                                   ttGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCtt                                                              <  1:735762/148‑1 (MQ=255)
                                                                                   ttGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCtt                                                              >  1:271613/1‑148 (MQ=255)
                                                                                   ttGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTAGATGGCCCGCGCGGCGCGGTGCtt                                                              >  1:2681495/1‑148 (MQ=255)
                                                                                    tGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCttt                                                             >  2:1633041/1‑148 (MQ=255)
                                                                                           ttCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCg                                                      <  2:1745601/148‑1 (MQ=255)
                                                                                             cTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGcc                                                    <  1:1606001/148‑1 (MQ=255)
                                                                                              ttgttgATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCa                                                   <  1:373375/148‑1 (MQ=255)
                                                                                              ttgttgATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCa                                                   <  1:2164624/148‑1 (MQ=255)
                                                                                               tgttgATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGTGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCag                                                  >  2:1010943/1‑148 (MQ=255)
                                                                                                   gatgatTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTg                                              <  1:3031986/148‑1 (MQ=255)
                                                                                                    atgatTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGa                                             <  1:212450/148‑1 (MQ=255)
                                                                                                    atgatTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGa                                             <  2:2079686/148‑1 (MQ=255)
                                                                                                     tgatTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGAt                                            <  1:1974623/148‑1 (MQ=255)
                                                                                                     tgatTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGAt                                            >  1:784067/1‑148 (MQ=255)
                                                                                                      gatTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATg                                           >  2:2437211/1‑148 (MQ=255)
                                                                                                         tttGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGc                                        >  2:7805/1‑148 (MQ=255)
                                                                                                                gCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAg                                 >  2:35946/1‑148 (MQ=255)
                                                                                                                gCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGACGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGACGCGAAg                                 >  2:2587082/1‑148 (MQ=255)
                                                                                                                    ttGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAgtggt                             >  2:2249133/1‑148 (MQ=255)
                                                                                                                    gtgctgcACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAgtggt                             <  2:3160829/147‑1 (MQ=255)
                                                                                                                     tgctgcACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCAGGGGCGGCTTGCTGTCGTTGACCAGTTCGATTGCCGGCGCGGCGCTGTCATTTTTAATATCCAGAGTTATGGGACGCGAAgaggtg                            >  2:1804939/1‑148 (MQ=255)
                                                                                                                        tgcACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGaa                         >  2:2819525/1‑148 (MQ=255)
                                                                                                                                 aCCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCTGCGAAGTGGTGGAAAAATTAAcc                <  2:816739/148‑1 (MQ=255)
                                                                                                                                 aCCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAAcc                <  2:2478841/148‑1 (MQ=255)
                                                                                                                                   cTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCgg              >  2:1760532/1‑148 (MQ=255)
                                                                                                                                   cTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCgg              >  2:2871821/1‑148 (MQ=255)
                                                                                                                                    tGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGc             >  2:3438794/1‑148 (MQ=255)
                                                                                                                                    tGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGc             >  1:1099782/1‑148 (MQ=255)
                                                                                                                                    tGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGc             >  2:1057298/1‑148 (MQ=255)
                                                                                                                                     gCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCa            >  2:1414947/1‑148 (MQ=255)
                                                                                                                                       gTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCaaa          <  1:871886/148‑1 (MQ=255)
                                                                                                                                       gTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCaaa          <  2:1241103/148‑1 (MQ=255)
                                                                                                                                       gTTTGTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTTCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCaaa          <  2:2311651/148‑1 (MQ=255)
                                                                                                                                         ttttGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGCAGTGGTGGAAAAATTAACCGGCAAAAc        >  2:266058/1‑148 (MQ=255)
                                                                                                                                         ttttGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAAc        >  2:3048115/1‑148 (MQ=255)
                                                                                                                                         ttttGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAAc        <  1:1337884/148‑1 (MQ=255)
                                                                                                                                         ttttGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAAc        >  2:2575861/1‑148 (MQ=255)
                                                                                                                                          tttGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGAACAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAAcc       <  1:642732/148‑1 (MQ=255)
                                                                                                                                            tGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGt     <  1:3472420/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCT  >  CP009273/1829175‑1829465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑