Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,833,730 C→A 100% R3S (CGT→AGT)  ynjE → molybdopterin synthase sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,833,7300CA99.0% 664.3 / ‑2.0 209R3S (CGT→AGT) ynjEmolybdopterin synthase sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base A (107/100);  total (107/102)
Fisher's exact test for biased strand distribution p-value = 2.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 210 total aligned reads displayed.

CCAGGATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTCGCCCCAGCGCGTTTT  >  CP009273/1833585‑1833874
                                                                                                                                                 |                                                                                                                                                
  aGGATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAgtgt                                                                                                                                              <  1:2137100/148‑1 (MQ=255)
   ggATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACCATGTCGGGATTTTCGATGAAAAGTGtt                                                                                                                                             >  2:1353975/1‑148 (MQ=255)
   ggATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGtt                                                                                                                                             >  1:961479/1‑148 (MQ=255)
   ggATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGtt                                                                                                                                             >  2:2845791/1‑148 (MQ=255)
    gATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGttt                                                                                                                                            <  2:754445/148‑1 (MQ=255)
    gATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGttt                                                                                                                                            <  1:1993701/148‑1 (MQ=255)
    gATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGttt                                                                                                                                            >  1:330727/1‑148 (MQ=255)
    gATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGttt                                                                                                                                            >  2:569617/1‑148 (MQ=255)
     aTGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTtc                                                                                                                                           <  2:2998547/148‑1 (MQ=255)
        ttCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCa                                                                                                                                        >  2:2318878/1‑148 (MQ=255)
        ttCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCa                                                                                                                                        >  2:2641788/1‑148 (MQ=255)
          ccTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCaaa                                                                                                                                      <  2:2524144/148‑1 (MQ=255)
            tgctgATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATg                                                                                                                                    <  2:1888804/148‑1 (MQ=255)
            tgctgATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATg                                                                                                                                    <  1:2471279/148‑1 (MQ=255)
            tgctgATAGTTCTGTTCTGGATATGGAGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATg                                                                                                                                    >  2:1698399/1‑148 (MQ=255)
              ctgATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGAc                                                                                                                                  <  2:1101211/148‑1 (MQ=255)
              ctgATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGAc                                                                                                                                  <  1:3550578/148‑1 (MQ=255)
                 aTAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACcgc                                                                                                                               >  2:3527499/1‑148 (MQ=255)
                 aTAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACcgc                                                                                                                               <  1:2739295/148‑1 (MQ=255)
                  tAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACcgcg                                                                                                                              >  1:3139914/1‑148 (MQ=255)
                  tAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACcgcg                                                                                                                              <  1:1045042/148‑1 (MQ=255)
                   aGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCgcgc                                                                                                                             >  2:2604108/1‑148 (MQ=255)
                   aGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCgcgc                                                                                                                             <  1:2366600/148‑1 (MQ=255)
                     ttctgttctgGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTg                                                                                                                           >  2:1024702/1‑148 (MQ=255)
                     ttctgttctgGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTg                                                                                                                           >  2:3130381/1‑148 (MQ=255)
                     ttctgttctgGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTg                                                                                                                           >  2:3045246/1‑148 (MQ=255)
                           tctgGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATg                                                                                                                     >  2:1370964/1‑148 (MQ=255)
                           tctgGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATg                                                                                                                     >  2:3139675/1‑148 (MQ=255)
                               gATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCtt                                                                                                                 >  2:2374421/1‑148 (MQ=255)
                                  atGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAg                                                                                                              <  1:2318878/148‑1 (MQ=255)
                                  atGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAg                                                                                                              <  1:1181865/148‑1 (MQ=255)
                                  atGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAg                                                                                                              <  1:2641788/148‑1 (MQ=255)
                                  atGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAg                                                                                                              >  2:1187478/1‑148 (MQ=255)
                                   tGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAgg                                                                                                             >  1:2467190/1‑148 (MQ=255)
                                      cgcAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGggct                                                                                                          <  1:1675527/148‑1 (MQ=255)
                                      cgcAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGggct                                                                                                          <  2:3461898/148‑1 (MQ=255)
                                          gTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGggctggct                                                                                                      >  2:2242856/1‑148 (MQ=255)
                                            ggTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTg                                                                                                    <  2:253126/148‑1 (MQ=255)
                                            ggTCAGAAAACTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTg                                                                                                    <  2:2960390/148‑1 (MQ=255)
                                                 gAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGctt                                                                                               >  2:2692458/1‑148 (MQ=255)
                                                  aaaaTTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGcttc                                                                                              >  1:3541203/1‑148 (MQ=255)
                                                   aaaTTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGcttct                                                                                             <  1:2205401/148‑1 (MQ=255)
                                                    aaTTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGcttctt                                                                                            >  2:307193/1‑148 (MQ=255)
                                                      ttACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCg                                                                                          <  2:2119702/148‑1 (MQ=255)
                                                      ttACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCg                                                                                          <  2:1561016/148‑1 (MQ=255)
                                                       tACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGt                                                                                         <  1:2524168/148‑1 (MQ=255)
                                                          aacaaACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTggg                                                                                      <  1:3008055/148‑1 (MQ=255)
                                                          aacaaACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTggg                                                                                      <  1:1232944/148‑1 (MQ=255)
                                                            caaACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGcc                                                                                    >  1:1813190/1‑148 (MQ=255)
                                                               aCTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCt                                                                                 >  1:13116/1‑148 (MQ=255)
                                                                   cGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAAc                                                                             <  2:1493217/148‑1 (MQ=255)
                                                                   cGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAAc                                                                             <  1:992059/148‑1 (MQ=255)
                                                                    gATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACt                                                                            <  1:2827638/148‑1 (MQ=255)
                                                                     aTAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTg                                                                           >  2:3271464/1‑148 (MQ=255)
                                                                     aTAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTg                                                                           <  1:197169/148‑1 (MQ=255)
                                                                      tAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTgg                                                                          >  1:431828/1‑148 (MQ=255)
                                                                       aaCGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGc                                                                         >  2:1576561/1‑148 (MQ=255)
                                                                        aCGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCg                                                                        <  1:2274338/148‑1 (MQ=255)
                                                                           cAAAGTTTATCTCAATTCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAg                                                                     >  1:3293382/1‑148 (MQ=255)
                                                                               gTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCctc                                                                 <  1:1187478/148‑1 (MQ=255)
                                                                               gTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCctc                                                                 <  1:2973064/148‑1 (MQ=255)
                                                                                         aTGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGa                                                       >  1:1727488/1‑148 (MQ=255)
                                                                                         aTGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGa                                                       >  1:3415451/1‑148 (MQ=255)
                                                                                              tCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGc                                                  <  2:2735918/148‑1 (MQ=255)
                                                                                                  ttCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTca                                              >  1:1439360/1‑148 (MQ=255)
                                                                                                     aGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTcaaca                                           <  2:1651845/148‑1 (MQ=255)
                                                                                                     aGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTcaaca                                           <  1:403310/148‑1 (MQ=255)
                                                                                                       aaTGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTcaacaac                                         >  1:1687192/1‑148 (MQ=255)
                                                                                                       aaTGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTcaacaac                                         >  1:791302/1‑148 (MQ=255)
                                                                                                        aTGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTcaacaaca                                        <  2:341784/148‑1 (MQ=255)
                                                                                                        aTGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTcaacaaca                                        <  2:516794/148‑1 (MQ=255)
                                                                                                          ggCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACaaa                                      <  1:1345591/148‑1 (MQ=255)
                                                                                                           gcgcACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACaaaa                                     <  1:1209355/148‑1 (MQ=255)
                                                                                                           gcgcACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACaaaa                                     >  1:2192398/1‑148 (MQ=255)
                                                                                                           gcgcACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACaaaa                                     <  1:101927/148‑1 (MQ=255)
                                                                                                            cgcACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAAt                                    >  1:86601/1‑148 (MQ=255)
                                                                                                              cACTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATgg                                  >  1:1880015/1‑148 (MQ=255)
                                                                                                               aCTCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGc                                 <  1:379960/148‑1 (MQ=255)
                                                                                                                 tCAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCaa                               <  1:893847/148‑1 (MQ=255)
                                                                                                                  cAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCaaa                              >  2:2106339/1‑148 (MQ=255)
                                                                                                                  cAAAACTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCaaa                              <  2:248370/148‑1 (MQ=255)
                                                                                                                     aaCTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCg                           >  2:2345893/1‑148 (MQ=255)
                                                                                                                     aaCTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCg                           <  1:1884265/148‑1 (MQ=255)
                                                                                                                      aCTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCga                          >  1:2903597/1‑148 (MQ=255)
                                                                                                                       cTACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCgat                         <  1:1698399/148‑1 (MQ=255)
                                                                                                                        tACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCgata                        >  2:146809/1‑148 (MQ=255)
                                                                                                                        tACAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCgata                        >  2:1291963/1‑148 (MQ=255)
                                                                                                                         aCAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCgatag                       >  2:1787598/1‑148 (MQ=255)
                                                                                                                          cAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCgataga                      >  2:1687738/1‑148 (MQ=255)
                                                                                                                          cAATGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGATTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCgataga                      <  1:566129/148‑1 (MQ=255)
                                                                                                                             tGTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATAc                   <  1:2294354/148‑1 (MQ=255)
                                                                                                                              gTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACt                  <  1:2103185/148‑1 (MQ=255)
                                                                                                                              gTCGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACt                  <  1:307193/148‑1 (MQ=255)
                                                                                                                                cGGGATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTCg                >  1:1138742/1‑148 (MQ=255)
                                                                                                                                cGGGATTTTCGATGAAAAGTGTTTCTCAAATGACAGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGTGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTCg                >  1:1770565/1‑148 (MQ=255)
                                                                                                                                   gATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTCGccc             >  1:2553608/1‑148 (MQ=255)
                                                                                                                                   gATTTTCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTCGccc             >  1:582428/1‑148 (MQ=255)
                                                                                                                                     ttttCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTCGCCCCa           <  1:2604108/148‑1 (MQ=255)
                                                                                                                                       ttCGATGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTCGCCCCAgc         >  2:2059783/1‑148 (MQ=255)
                                                                                                                                            tGAAAAGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTCGCCCCAGCGCGtt    <  1:3204765/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCAGGATGTTCCTGCTGATAGTTCTGTTCTGGATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTCGCCCCAGCGCGTTTT  >  CP009273/1833585‑1833874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑