Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,836,192 T→A 100% I67I (ATA→ATT)  ynjH ← DUF1496 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,836,1920TA97.4% 499.0 / 2.6 160I67I (ATA→ATT) ynjHDUF1496 family protein
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base A (75/80);  total (78/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 160 total aligned reads displayed.

GGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGG  >  CP009273/1836049‑1836335
                                                                                                                                               |                                                                                                                                               
  tctggtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCt                                                                                                                                           <  2:234569/148‑1 (MQ=255)
  tctggtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCt                                                                                                                                           <  1:1613664/148‑1 (MQ=255)
      gtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTATTGCTAAGGGTTTTGTCATCACGCTGGCCGTGGAGCAGAATTCCTTCgg                                                                                                                                       >  2:2318000/1‑148 (MQ=255)
      gtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCgg                                                                                                                                       >  2:3077373/1‑148 (MQ=255)
       tGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTTAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGc                                                                                                                                      <  1:3270559/148‑1 (MQ=255)
        gCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCCTTGCAGCAGAATTCCTTCGGCt                                                                                                                                     >  2:3112599/1‑148 (MQ=255)
        gCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCt                                                                                                                                     <  1:393290/148‑1 (MQ=255)
        gCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCt                                                                                                                                     <  2:2034861/148‑1 (MQ=255)
         cTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCtt                                                                                                                                    >  2:1573349/1‑148 (MQ=255)
         cTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCtt                                                                                                                                    >  2:210131/1‑148 (MQ=255)
            aCCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTaa                                                                                                                                 <  2:2843615/148‑1 (MQ=255)
              cTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAAtt                                                                                                                               <  2:1872841/148‑1 (MQ=255)
               tGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTTTTGCTTTAAGCGCCCCCACACCAGCCGCTTAGTGCTAATGGGTTTGTAATCAACCGGGCCATTCAGCACAAATACTTCGGCGTTAATTa                                                                                                                              >  1:712805/1‑148 (MQ=255)
                gAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTAc                                                                                                                             <  1:1759257/148‑1 (MQ=255)
                gAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTAc                                                                                                                             <  1:1759469/148‑1 (MQ=255)
                gAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCGTTACGCGCCGCCAGACCAGCGGCTTAGTGCGAAGGGGTTTGTGGTCACGCGGGCATTGCCGCAGAATTCCTTCGGCGGTAATTaa                                                                                                                             >  1:1361491/1‑147 (MQ=255)
                  agagGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCCCGCTGGCATTGCAGCAGGATTCCTTCGGCTTTACTTAACg                                                                                                                           >  2:2895830/1‑148 (MQ=255)
                  agagGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTGTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCCTCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTAACg                                                                                                                           >  2:2895857/1‑148 (MQ=255)
                    agGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGGGTATGGCTTTACGCGCCGCCAGACCAGCCGCTTAGTTGTAAGGGTTTTGTCATCACGCTGGCAATGCCGCAGAATTCCATCGGCGTTAATTAACGcc                                                                                                                         >  2:379089/1‑148 (MQ=255)
                      gcgcTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGcccc                                                                                                                       <  1:2795772/148‑1 (MQ=255)
                        gcTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCtt                                                                                                                     <  2:687009/148‑1 (MQ=255)
                        gcTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCtt                                                                                                                     <  2:2470762/148‑1 (MQ=255)
                         cTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATTGGTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCAGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTc                                                                                                                    >  2:361510/1‑148 (MQ=255)
                         cTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTc                                                                                                                    <  2:302976/148‑1 (MQ=255)
                         cTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTc                                                                                                                    <  1:106782/148‑1 (MQ=255)
                          tGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCTGCAGAATTCCTTCGGCTTTAATTACCGCCCCttga                                                                                                                   >  2:1613432/1‑146 (MQ=255)
                          tGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTGTATGGCGTTACGCGCCCCCAGACCAGCGGCTTAGTGCTAAGGGTTTTGTCATCACGCTGGCCTTGCAGCAGAATTCCTTCGGCGTTAATTACCGCCCCATCa                                                                                                                   >  1:75168/1‑148 (MQ=255)
                              atatCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTAGGCTTTAATTACCGCCCCGTCAGaat                                                                                                               >  1:3546641/1‑148 (MQ=255)
                              atatCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCAATGCAGCAGAATTCCTTCGGCGTTAATTAACGCCCCATCAGaag                                                                                                               >  1:540463/1‑147 (MQ=255)
                                atCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGGTTTAAGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTTTCATAAAGCTGGCATTGCAGCAGAAATCCTTCGGCTTTAATTACCGCCCCGTCAGcataa                                                                                                             >  2:2697098/1‑148 (MQ=255)
                                 tCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGaataat                                                                                                            >  2:857632/1‑148 (MQ=255)
                                 tCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTAAGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTTTCATAACGCTGGCATTGCAGCAGAATTCCATCGGCTTTAATTAACGCCCCTTCAGaataaa                                                                                                            >  2:309815/1‑147 (MQ=255)
                                  ccGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAAtt                                                                                                           <  2:1078514/148‑1 (MQ=255)
                                   cGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAAttt                                                                                                          <  2:1905112/148‑1 (MQ=255)
                                   cGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAAttt                                                                                                          <  1:744249/148‑1 (MQ=255)
                                     cTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTTTTGCTTTAAGCGCCGCCAGACCAGCGGATTATTGCTAAGGGGTTTGTGATCAAGCTGGCAATGCAGCAGAAATACTTCGGCGTTAATTACCGCCCCCTAAGAATAAttttt                                                                                                        >  1:17953/1‑147 (MQ=255)
                                     cTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTg                                                                                                        <  1:904974/148‑1 (MQ=255)
                                        gCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATc                                                                                                     <  1:995812/148‑1 (MQ=255)
                                          cccTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATccg                                                                                                   >  2:3367282/1‑147 (MQ=255)
                                           ccTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTg                                                                                                  <  2:1969141/148‑1 (MQ=255)
                                               ctgACATTCCATTATTAGAGGCGTTTATTGCTTTACGCGCCGCCAGACCAGCGGATTAGTGGTAAGGGTTTTGTCATAACGCTGGCATTGCAGCAGAATTCCATCGGCTTTAATTAACGCCCCTTGAGAATAATTTTGATACTGATaa                                                                                              >  2:1035984/1‑148 (MQ=255)
                                               ctgACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATaa                                                                                              <  2:463839/148‑1 (MQ=255)
                                                tgACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATaaa                                                                                             <  1:318565/148‑1 (MQ=255)
                                                   cATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACgc                                                                                          <  2:854336/148‑1 (MQ=255)
                                                     ttCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACgcag                                                                                        <  2:3170971/148‑1 (MQ=255)
                                                     ttCCATTATTAGAGGCGTTTATGGCTTTAAGCGCCGCCACACCACCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCAATTCAGCAGAATTCCTTCGGCTTTAATTACCCCCCCATCAGAATAATTTTGATCCATATAAAAgcag                                                                                        >  2:2754943/1‑148 (MQ=255)
                                                      tCCATTATTAGAGGCGTTTATTGCTTTACGCGCCGCCAGACCAGCGGCTTAGTGCTAAGGGTTTTTTCATAACGCTGGCATTGCAGCAGAAATACTTCGGCTTTAATTACCGCCCCATAAGCATAATTTTTTTGCTGATAAACccagc                                                                                       >  1:2915833/1‑148 (MQ=255)
                                                      tCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTAGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACgcagc                                                                                       <  2:2236846/148‑1 (MQ=255)
                                                           tattaGAGGCGTTTATTGCTTTAAGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAAATACATCGGCTTTAATTACCGCCCCTTCAGAATAAATTTTATACTGATAAAAGCAGCACTGa                                                                                  >  2:950975/1‑148 (MQ=255)
                                                           tattaGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGa                                                                                  <  1:1339462/148‑1 (MQ=255)
                                                            attaGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAg                                                                                 <  2:1492824/148‑1 (MQ=255)
                                                            attaGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAg                                                                                 <  1:1987792/148‑1 (MQ=255)
                                                            attaGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAAAgcagcaaagag                                                                                 >  2:3215069/1‑143 (MQ=255)
                                                                 agGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAg                                                                            <  1:2843443/148‑1 (MQ=255)
                                                                  ggCGTTTATTGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTTTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTAACGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAgg                                                                           >  1:2535806/1‑148 (MQ=255)
                                                                   gCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAggg                                                                          <  2:3418523/148‑1 (MQ=255)
                                                                   gCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAggg                                                                          <  2:2208825/148‑1 (MQ=255)
                                                                       ttATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTgc                                                                      >  1:3467195/1‑148 (MQ=255)
                                                                       ttATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTgc                                                                      >  2:2178561/1‑148 (MQ=255)
                                                                            gCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTGCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGa                                                                 >  2:524566/1‑148 (MQ=255)
                                                                               ttACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTg                                                              >  2:701647/1‑148 (MQ=255)
                                                                                         ccAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCctgaa                                                    >  1:1583503/1‑148 (MQ=255)
                                                                                            gACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCctgaactg                                                 >  1:2266353/1‑148 (MQ=255)
                                                                                               cAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCtgaactgaac                                              >  2:1476502/1‑148 (MQ=255)
                                                                                                  cGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACt                                           <  2:1370947/148‑1 (MQ=255)
                                                                                                     aTTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCt                                        >  1:1411065/1‑148 (MQ=255)
                                                                                                       tAGTGCTAAGGTTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCATAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTgg                                      <  1:834473/148‑1 (MQ=255)
                                                                                                         gTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCg                                    >  1:2233710/1‑148 (MQ=255)
                                                                                                          tGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCATAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCgg                                   <  1:3077373/148‑1 (MQ=255)
                                                                                                          tGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCgg                                   >  2:2182793/1‑148 (MQ=255)
                                                                                                          tGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCgg                                   >  1:1102389/1‑148 (MQ=255)
                                                                                                          tGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCgg                                   >  1:1102639/1‑148 (MQ=255)
                                                                                                           gCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGATTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAACCGCAGCACTGAGTACCGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGt                                  <  1:1635082/148‑1 (MQ=255)
                                                                                                           gCTAAGGGTTTTGTCAACACGCGGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCGGATAACCGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGt                                  <  1:379089/148‑1 (MQ=255)
                                                                                                              aaGGGTTTTGTCAACACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACg                               <  1:3064872/148‑1 (MQ=255)
                                                                                                               aGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGt                              >  1:1037650/1‑148 (MQ=255)
                                                                                                                  gTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTa                           >  1:880459/1‑148 (MQ=255)
                                                                                                                   ttttGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTAc                          >  2:3281436/1‑148 (MQ=255)
                                                                                                                    tttGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTAcc                         >  1:1649516/1‑148 (MQ=255)
                                                                                                                      tGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCATAATAATTTTGATCCTGATAAACGCAGCACTGAGTACTGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTc                       <  1:1573349/148‑1 (MQ=255)
                                                                                                                      tGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACCGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTc                       <  1:210131/148‑1 (MQ=255)
                                                                                                                      tGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTc                       >  1:1312097/1‑148 (MQ=255)
                                                                                                                      tGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTc                       <  2:759414/148‑1 (MQ=255)
                                                                                                                       gTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTcc                      <  1:152015/148‑1 (MQ=255)
                                                                                                                        tcatcaCGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCCGCACTGAGTACCGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCa                     <  1:1592070/148‑1 (MQ=255)
                                                                                                                           tcaCGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGt                  >  2:843403/1‑148 (MQ=255)
                                                                                                                            caCGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTc                 <  2:2282127/148‑1 (MQ=255)
                                                                                                                            caCGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTc                 <  2:931638/148‑1 (MQ=255)
                                                                                                                             aCGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAACCGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTcc                <  2:1783382/148‑1 (MQ=255)
                                                                                                                             aCGCTGGCATTGCAGCAGAAGTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACGTCTGGCGGTACGTTTACCTCCACGTcc                <  2:1783354/148‑1 (MQ=255)
                                                                                                                                cTGGCATTGCAGCAGTATTCCTTCGGCATTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGa             <  2:3451800/148‑1 (MQ=255)
                                                                                                                                 tGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGAc            >  1:3249879/1‑148 (MQ=255)
                                                                                                                                 tGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGAc            >  1:3249829/1‑148 (MQ=255)
                                                                                                                                  ggCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACg           >  1:597122/1‑148 (MQ=255)
                                                                                                                                  ggCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACg           <  1:2354174/148‑1 (MQ=255)
                                                                                                                                      ttGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATaa       <  2:540463/148‑1 (MQ=255)
                                                                                                                                       tGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAAt      >  1:340152/1‑148 (MQ=255)
                                                                                                                                           gcagAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCgg  >  1:3281965/1‑148 (MQ=255)
                                                                                                                                           gcagAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCgg  >  2:2798187/1‑148 (MQ=255)
                                                                                                                                           gcagAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCgg  >  2:2798177/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                               
GGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGG  >  CP009273/1836049‑1836335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑