Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,855,239 A→T 9.4% N117K (AAT→AAA)  ydjL ← putative Zn‑dependent NAD(P)‑binding oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,855,2390AT 9.4% 522.8 / 37.6 181N117K (AAT→AAA) ydjLputative Zn‑dependent NAD(P)‑binding oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (81/82);  new base T (7/10);  total (89/92)
Fisher's exact test for biased strand distribution p-value = 6.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

Only 100 of 201 total aligned reads displayed.

GATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCAACCTG  >  CP009273/1855092‑1855385
                                                                                                                                                   |                                                                                                                                                  
gATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGta                                                                                                                                                    <  1:2689267/148‑1 (MQ=255)
gATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGta                                                                                                                                                    <  1:3371841/148‑1 (MQ=255)
  tGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGtatt                                                                                                                                                  <  1:2414814/148‑1 (MQ=255)
   ggATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGtttta                                                                                                                                                 >  1:1275871/1‑148 (MQ=255)
   ggATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGtatta                                                                                                                                                 >  1:153011/1‑148 (MQ=255)
   ggATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGtatta                                                                                                                                                 >  2:2205734/1‑148 (MQ=255)
    gATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGttttat                                                                                                                                                <  2:2363352/148‑1 (MQ=255)
    gATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGttttat                                                                                                                                                <  2:3413782/148‑1 (MQ=255)
     aTTTGTAGGCATTACAGATTGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCCGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGtattatt                                                                                                                                               <  1:307762/148‑1 (MQ=255)
       ttGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTAGGAAAAACCACCGCCCCAGGtattattat                                                                                                                                             >  2:1951470/1‑148 (MQ=255)
        tGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATc                                                                                                                                            <  1:1410260/148‑1 (MQ=255)
            ggCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGa                                                                                                                                        >  1:2327852/1‑148 (MQ=255)
             gCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGAc                                                                                                                                       >  1:865258/1‑148 (MQ=255)
               aTTACTGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATAAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCTCCGCCCCAGGTATTATTATCCAGACCa                                                                                                                                     <  2:2437396/148‑1 (MQ=255)
                 tACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAAACACCGCCCCAGGTATTATTATCCAGACCAAg                                                                                                                                   >  2:1754140/1‑148 (MQ=255)
                    aGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGtt                                                                                                                                >  1:428107/1‑148 (MQ=255)
                      aTAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTa                                                                                                                              >  2:1386391/1‑148 (MQ=255)
                         gggTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACtt                                                                                                                           >  2:68288/1‑148 (MQ=255)
                          ggTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACttt                                                                                                                          >  2:1422522/1‑148 (MQ=255)
                          ggTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACttt                                                                                                                          >  2:2652680/1‑148 (MQ=255)
                           gTCAAGTACGGCTGCGTCCTCATAATCAACCCCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACtttt                                                                                                                         <  1:2523232/148‑1 (MQ=255)
                           gTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCAACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACtttt                                                                                                                         <  2:2625268/148‑1 (MQ=255)
                            tCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACttttt                                                                                                                        <  2:1678984/148‑1 (MQ=255)
                            tCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACttttt                                                                                                                        <  2:2292109/148‑1 (MQ=255)
                            tCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACttttt                                                                                                                        <  1:1091440/148‑1 (MQ=255)
                            tCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTAGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACttttt                                                                                                                        <  2:222070/148‑1 (MQ=255)
                            tCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGCGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACttttt                                                                                                                        <  2:2508092/148‑1 (MQ=255)
                               aGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTg                                                                                                                     <  2:2978555/148‑1 (MQ=255)
                                  aCGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTac                                                                                                                  >  1:399674/1‑148 (MQ=255)
                                    ggCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTacaa                                                                                                                <  1:3055139/148‑1 (MQ=255)
                                     gCTGCGTCCTCATAATCAACACCCTCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTacaac                                                                                                               <  1:1103652/148‑1 (MQ=255)
                                     gCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTacaac                                                                                                               <  1:3249588/148‑1 (MQ=255)
                                     gCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTacaac                                                                                                               <  1:3385368/148‑1 (MQ=255)
                                      cTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTacaaca                                                                                                              <  2:2248099/148‑1 (MQ=255)
                                       tGCGTCCTCATACTCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAacac                                                                                                             <  1:1380139/148‑1 (MQ=255)
                                             cTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGaaaa                                                                                                       <  1:798617/148‑1 (MQ=255)
                                              tCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAAt                                                                                                      >  1:2873116/1‑148 (MQ=255)
                                              tCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTAGAGAATTTCACCAGGAACCAGACAATATATGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTATTCTGTACAACACAGAAAAt                                                                                                      >  1:1149176/1‑148 (MQ=255)
                                                aTAATCAACCCCATCAGGGATTTCCCACAACGCAGGACGATGAATTTTGAGACTTTCACCAGGAACCAGACAATCTTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCa                                                                                                    <  2:572374/148‑1 (MQ=255)
                                                 tAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCAc                                                                                                   >  1:2116337/1‑148 (MQ=255)
                                                 tAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCAc                                                                                                   >  2:2048773/1‑148 (MQ=255)
                                                  aaTCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCAcc                                                                                                  <  1:2622147/148‑1 (MQ=255)
                                                  aaTCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCAcc                                                                                                  >  2:833485/1‑148 (MQ=255)
                                                  aaTCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCAcc                                                                                                  <  1:1710643/148‑1 (MQ=255)
                                                  aaTCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCAcc                                                                                                  <  1:1490106/148‑1 (MQ=255)
                                                    tCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCtt                                                                                                >  2:1210250/1‑148 (MQ=255)
                                                    tCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTAGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCtt                                                                                                <  1:1726762/148‑1 (MQ=255)
                                                    tCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTAGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCtt                                                                                                >  1:2802628/1‑148 (MQ=255)
                                                    tCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTAGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCtt                                                                                                >  1:1024305/1‑148 (MQ=255)
                                                     cAACACCATCAGGGATTTCCCACCACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTg                                                                                               >  1:533378/1‑148 (MQ=255)
                                                     cAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTg                                                                                               <  1:1977300/148‑1 (MQ=255)
                                                          ccATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTcac                                                                                          <  2:1167618/148‑1 (MQ=255)
                                                            aTCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAg                                                                                        >  1:3507000/1‑148 (MQ=255)
                                                            aTCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAg                                                                                        >  2:1648217/1‑148 (MQ=255)
                                                                 ggATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCgg                                                                                   <  1:2016965/148‑1 (MQ=255)
                                                                 ggATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCgg                                                                                   <  2:2094850/148‑1 (MQ=255)
                                                                  gATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAGTCACCTTGTTCACAGGCCGGa                                                                                  >  2:1038439/1‑148 (MQ=255)
                                                                  gATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTAGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGa                                                                                  <  2:1430009/148‑1 (MQ=255)
                                                                    tttCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACa                                                                                >  1:193458/1‑148 (MQ=255)
                                                                     ttCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACaa                                                                               >  2:2803311/1‑148 (MQ=255)
                                                                     ttCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACaa                                                                               >  2:473601/1‑148 (MQ=255)
                                                                      tCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACaaa                                                                              <  2:2818276/148‑1 (MQ=255)
                                                                      tCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACaaa                                                                              <  2:210564/148‑1 (MQ=255)
                                                                       cccACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAac                                                                             <  1:3230749/148‑1 (MQ=255)
                                                                         cacaACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTAGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAacac                                                                           >  1:1628763/1‑148 (MQ=255)
                                                                              cGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCaa                                                                      <  1:2850919/148‑1 (MQ=255)
                                                                                   gACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTg                                                                 <  2:58506/148‑1 (MQ=255)
                                                                                   gACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTg                                                                 <  2:153011/148‑1 (MQ=255)
                                                                                    aCGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGa                                                                >  2:3548176/1‑148 (MQ=255)
                                                                                    aCGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGa                                                                >  2:3472932/1‑148 (MQ=255)
                                                                                    aCGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGa                                                                >  2:1790837/1‑148 (MQ=255)
                                                                                        tGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGc                                                            <  1:3357114/148‑1 (MQ=255)
                                                                                            ttttGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGtt                                                        <  1:2514647/148‑1 (MQ=255)
                                                                                                   aaTTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGAt                                                 <  1:2722628/148‑1 (MQ=255)
                                                                                                           cAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGAcgcg                                         >  2:1964491/1‑148 (MQ=255)
                                                                                                           cAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGAcgcg                                         <  1:61463/148‑1 (MQ=255)
                                                                                                            aGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGt                                        <  2:2095083/148‑1 (MQ=255)
                                                                                                              gAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTg                                      >  1:3414754/1‑148 (MQ=255)
                                                                                                              gAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTACTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTg                                      >  1:3415814/1‑148 (MQ=255)
                                                                                                                aCCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGcc                                    <  2:3371028/148‑1 (MQ=255)
                                                                                                                   aGACAATATTAGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCa                                 >  2:3066032/1‑148 (MQ=255)
                                                                                                                        atatTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTc                            >  2:380163/1‑148 (MQ=255)
                                                                                                                           tttGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAg                         >  2:795962/1‑148 (MQ=255)
                                                                                                                            ttGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGt                        >  1:3219852/1‑148 (MQ=255)
                                                                                                                               gAAAAACCACCGCCCCAGGTCTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCtt                     >  2:110416/1‑148 (MQ=255)
                                                                                                                               gAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCtt                     >  2:531539/1‑148 (MQ=255)
                                                                                                                                 aaaaCCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTg                   >  2:2934641/1‑148 (MQ=255)
                                                                                                                                  aaaCCACCGCCCCAGGTATTATTATCCAGACAAAGGTTTACTTTTTATGTACAACACAGAAAATCAACTTCTTCACAGGCCGTAAAAACACCGAAAACGTGACCCCACTTATCCGATACGACTCTTTGACCCACTATCAAGTCATTGa                  >  1:1203810/1‑148 (MQ=255)
                                                                                                                                    accaccGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACt                >  2:2042459/1‑148 (MQ=255)
                                                                                                                                     ccaccGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACtt               <  1:2669822/148‑1 (MQ=255)
                                                                                                                                     ccaccGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACtt               >  2:320159/1‑148 (MQ=255)
                                                                                                                                     ccaccGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACtt               <  2:3507000/148‑1 (MQ=255)
                                                                                                                                       accGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACtttt             >  2:1398937/1‑148 (MQ=255)
                                                                                                                                             ccAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCa       >  2:2232899/1‑148 (MQ=255)
                                                                                                                                             ccAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCa       <  1:478521/148‑1 (MQ=255)
                                                                                                                                             ccAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCa       >  2:836692/1‑148 (MQ=255)
                                                                                                                                              cAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCaa      <  1:832777/148‑1 (MQ=255)
                                                                                                                                                ggTTTTATTACCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCAAcc    <  2:1275871/148‑1 (MQ=255)
                                                                                                                                                  tattattatCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCAACCTg  >  1:2913563/1‑148 (MQ=255)
                                                                                                                                                  tattattatCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCAACCTg  >  2:415016/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCAACCTG  >  CP009273/1855092‑1855385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑