Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,864,305 T→G 5.1% L365* (TTA→TGA)  yeaH → UPF0229 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,864,3050TG 5.1% 625.8 / 23.4 214L365* (TTA→TGA) yeaHUPF0229 family protein
Reads supporting (aligned to +/- strand):  ref base T (97/106);  new base G (7/4);  total (104/110)
Fisher's exact test for biased strand distribution p-value = 3.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

Only 100 of 226 total aligned reads displayed.

GTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTT  >  CP009273/1864160‑1864452
                                                                                                                                                 |                                                                                                                                                   
  ttCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAAttatta                                                                                                                                                 <  1:484731/148‑1 (MQ=255)
  ttCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAAttatta                                                                                                                                                 >  2:276872/1‑148 (MQ=255)
     cAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCt                                                                                                                                              <  2:2004273/148‑1 (MQ=255)
          cccTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATGATTACCtgttgt                                                                                                                                         >  2:1448875/1‑148 (MQ=255)
            cTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTc                                                                                                                                       <  1:556652/148‑1 (MQ=255)
             tGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCg                                                                                                                                      <  2:2290681/148‑1 (MQ=255)
             tGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCg                                                                                                                                      <  2:1562718/148‑1 (MQ=255)
              gAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGt                                                                                                                                     <  2:1678813/148‑1 (MQ=255)
              gAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGt                                                                                                                                     >  2:2131793/1‑148 (MQ=255)
               aaaCTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGtt                                                                                                                                    >  1:2224490/1‑148 (MQ=255)
                aaCTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGtta                                                                                                                                   <  1:3024371/148‑1 (MQ=255)
                aaCTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGtta                                                                                                                                   <  1:1610626/148‑1 (MQ=255)
                aaCTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATGATTACCTGTTGTTCGtta                                                                                                                                   >  1:2156124/1‑148 (MQ=255)
                     atggatgAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACa                                                                                                                              <  2:3063163/148‑1 (MQ=255)
                      tggatgAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAg                                                                                                                             >  2:1910739/1‑148 (MQ=255)
                      tggatgAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAg                                                                                                                             <  2:1148438/148‑1 (MQ=255)
                      tggatgAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAg                                                                                                                             <  2:2990619/148‑1 (MQ=255)
                       ggatgAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTTCGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGc                                                                                                                            >  1:1551137/1‑148 (MQ=255)
                           gAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCtata                                                                                                                        <  1:3219348/148‑1 (MQ=255)
                           gAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCtata                                                                                                                        >  1:1533032/1‑148 (MQ=255)
                           gAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCtata                                                                                                                        <  1:1110830/148‑1 (MQ=255)
                              gTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCg                                                                                                                     >  1:1571823/1‑148 (MQ=255)
                              gTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCg                                                                                                                     >  1:843450/1‑148 (MQ=255)
                                 gTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGaaa                                                                                                                  >  2:1001724/1‑148 (MQ=255)
                                  tGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAAt                                                                                                                 >  1:1716672/1‑148 (MQ=255)
                                  tGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAAt                                                                                                                 >  1:3532057/1‑148 (MQ=255)
                                     aaGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTAc                                                                                                              <  2:684117/148‑1 (MQ=255)
                                     aaGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATCACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATACCGAAATTAc                                                                                                              <  2:259095/148‑1 (MQ=255)
                                      agagCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTAcc                                                                                                             >  1:927135/1‑148 (MQ=255)
                                             tataACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTg                                                                                                      <  2:335299/148‑1 (MQ=255)
                                                 aCCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGcaca                                                                                                  >  2:3266121/1‑148 (MQ=255)
                                                 aCCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGcaca                                                                                                  >  1:174077/1‑148 (MQ=255)
                                                  cccGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACAt                                                                                                 >  1:3022391/1‑148 (MQ=255)
                                                   ccGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATc                                                                                                >  1:2454224/1‑148 (MQ=255)
                                                    cGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCa                                                                                               >  2:831676/1‑148 (MQ=255)
                                                          aGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACAtt                                                                                         >  1:3123685/1‑148 (MQ=255)
                                                              gAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGACAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGACATTACCCGTCGTGCACATCAGACATTGTgg                                                                                     >  2:2919578/1‑148 (MQ=255)
                                                              gAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTgg                                                                                     <  2:619400/148‑1 (MQ=255)
                                                                atatTTACGCTGCACACGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCg                                                                                   <  2:2185666/148‑1 (MQ=255)
                                                                atatTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCg                                                                                   <  2:3366442/148‑1 (MQ=255)
                                                                atatTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCg                                                                                   <  2:1114898/148‑1 (MQ=255)
                                                                 tatTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCga                                                                                  >  1:257341/1‑148 (MQ=255)
                                                                  atTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCgag                                                                                 >  1:3491573/1‑148 (MQ=255)
                                                                  atTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCgag                                                                                 >  1:3371160/1‑148 (MQ=255)
                                                                  atTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCgag                                                                                 >  1:2378145/1‑148 (MQ=255)
                                                                   tttACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCgaga                                                                                <  1:1579762/148‑1 (MQ=255)
                                                                     tACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAat                                                                              <  2:2749620/148‑1 (MQ=255)
                                                                       cGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAACAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAatat                                                                            >  1:738169/1‑148 (MQ=255)
                                                                       cGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAatat                                                                            <  1:1823420/148‑1 (MQ=255)
                                                                             acaAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCAt                                                                      <  1:2727319/148‑1 (MQ=255)
                                                                             acaAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCAt                                                                      <  1:202515/148‑1 (MQ=255)
                                                                                aGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTg                                                                   <  1:2542905/148‑1 (MQ=255)
                                                                                  cATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCa                                                                 <  1:523238/148‑1 (MQ=255)
                                                                                    tCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAAt                                                               <  1:110169/148‑1 (MQ=255)
                                                                                    tCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAAt                                                               >  1:3078272/1‑148 (MQ=255)
                                                                                       gACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTa                                                            >  1:1162062/1‑148 (MQ=255)
                                                                                       gACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTa                                                            >  1:1231820/1‑148 (MQ=255)
                                                                                        aCGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTAc                                                           <  2:2470123/148‑1 (MQ=255)
                                                                                        aCGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTAc                                                           <  2:2504552/148‑1 (MQ=255)
                                                                                        aCGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTAc                                                           <  1:2162474/148‑1 (MQ=255)
                                                                                        aCGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTAc                                                           <  2:1791409/148‑1 (MQ=255)
                                                                                         cGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACt                                                          <  2:257341/148‑1 (MQ=255)
                                                                                         cGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACt                                                          <  2:534167/148‑1 (MQ=255)
                                                                                             gATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCg                                                      <  1:2704552/148‑1 (MQ=255)
                                                                                              aTAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGa                                                     <  2:444482/148‑1 (MQ=255)
                                                                                                 aCTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACaa                                                  <  2:1105834/148‑1 (MQ=255)
                                                                                                 aCTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCCACaa                                                  >  1:2329359/1‑148 (MQ=255)
                                                                                                  cTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAAc                                                 <  2:588270/148‑1 (MQ=255)
                                                                                                   tGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACt                                                <  2:2257389/148‑1 (MQ=255)
                                                                                                    gggCCGATGACTCTCCTATTTGCCATGAAATCCTGTCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCAAATCAGACATTGTGGAGAGAATATGAGCATCTGCAATCTACTTTCGACAACtt                                               <  1:3220826/148‑1 (MQ=255)
                                                                                                     ggCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACttt                                              >  2:919910/1‑148 (MQ=255)
                                                                                                     ggCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACttt                                              >  2:919977/1‑148 (MQ=255)
                                                                                                      gCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTg                                             >  1:3017545/1‑148 (MQ=255)
                                                                                                          aTGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGAt                                         <  2:3491573/148‑1 (MQ=255)
                                                                                                            gACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATgc                                       >  1:408735/1‑148 (MQ=255)
                                                                                                                    gCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATc                               <  2:805997/148‑1 (MQ=255)
                                                                                                                      tttGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCcg                             >  2:2633774/1‑148 (MQ=255)
                                                                                                                           cATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGAcc                        <  2:3364622/148‑1 (MQ=255)
                                                                                                                            aTGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGAccc                       >  2:1312175/1‑147 (MQ=255)
                                                                                                                            aTGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCa                       >  2:2726172/1‑148 (MQ=255)
                                                                                                                             tGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAg                      >  1:1293214/1‑148 (MQ=255)
                                                                                                                             tGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAg                      >  2:1336703/1‑148 (MQ=255)
                                                                                                                              gAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAgg                     >  2:3321540/1‑148 (MQ=255)
                                                                                                                               aaaTCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGga                    >  2:139056/1‑148 (MQ=255)
                                                                                                                               aaaTCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGga                    <  1:3258862/148‑1 (MQ=255)
                                                                                                                                 aTCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGgatg                  <  1:1420097/148‑1 (MQ=255)
                                                                                                                                 aTCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGgatg                  <  1:383233/148‑1 (MQ=255)
                                                                                                                                  tCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGgatga                 >  2:1598466/1‑148 (MQ=255)
                                                                                                                                   ccTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGgatgat                >  2:1806059/1‑148 (MQ=255)
                                                                                                                                   ccTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGgatgat                >  1:958021/1‑148 (MQ=255)
                                                                                                                                       gCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATAttt            <  2:2702216/148‑1 (MQ=255)
                                                                                                                                       gCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATAttt            <  1:1001724/148‑1 (MQ=255)
                                                                                                                                        cGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTa           >  2:191776/1‑148 (MQ=255)
                                                                                                                                         gAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAGATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTAt          <  2:1123972/148‑1 (MQ=255)
                                                                                                                                         gAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTAt          <  2:1551137/148‑1 (MQ=255)
                                                                                                                                            aaaaTTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCg       >  1:2807795/1‑148 (MQ=255)
                                                                                                                                             aaaTTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCgg      >  2:1987167/1‑148 (MQ=255)
                                                                                                                                              aaTTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGgt     <  1:1910739/148‑1 (MQ=255)
                                                                                                                                              aaTTATTACCTGTTGTTAGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGgt     >  2:1255028/1‑148 (MQ=255)
                                                                                                                                               attattACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGgtg    <  2:3531818/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTT  >  CP009273/1864160‑1864452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑