Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,899,496 C→T 7.3% L147F (CTT→TTT)  yobD → UPF0266 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,899,4960CT 7.3% 806.6 / 33.0 231L147F (CTT→TTT) yobDUPF0266 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (94/120);  new base T (6/11);  total (100/131)
Fisher's exact test for biased strand distribution p-value = 6.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 235 total aligned reads displayed.

GGTTTTTTCTTCGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGAAAA  >  CP009273/1899349‑1899643
                                                                                                                                                   |                                                                                                                                                   
     tttCTTCGCCAATGTCTGGATTTAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTtctc                                                                                                                                                <  1:74823/148‑1 (MQ=255)
     tttCTTCGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTtctc                                                                                                                                                <  1:3246520/148‑1 (MQ=255)
       tcttcGCCATTGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGTTGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGt                                                                                                                                              <  1:928962/148‑1 (MQ=255)
       tcttcGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGt                                                                                                                                              <  2:3477195/148‑1 (MQ=255)
           cGCCAATGTCTGGATTTAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCa                                                                                                                                          <  1:2487287/148‑1 (MQ=255)
            gCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCaa                                                                                                                                         >  2:2163179/1‑148 (MQ=255)
            gCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCaa                                                                                                                                         >  2:214055/1‑148 (MQ=255)
             ccAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAAc                                                                                                                                        <  1:3363370/148‑1 (MQ=255)
               aaTGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTc                                                                                                                                      >  1:707127/1‑148 (MQ=255)
                aTGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGTTTCTCGTTTCAACTCa                                                                                                                                     >  1:217817/1‑148 (MQ=255)
                 tGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCaa                                                                                                                                    >  2:375125/1‑148 (MQ=255)
                      ggATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGt                                                                                                                               >  2:1299957/1‑148 (MQ=255)
                       gATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGtt                                                                                                                              >  2:747399/1‑148 (MQ=255)
                        aTTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTa                                                                                                                             <  1:2776526/148‑1 (MQ=255)
                          tGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATg                                                                                                                           >  1:1412572/1‑148 (MQ=255)
                          tGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATg                                                                                                                           >  2:574826/1‑148 (MQ=255)
                            aaTATAGCAGAATAAAAGAGATGAACTTGTCTGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGaa                                                                                                                         <  2:260789/148‑1 (MQ=255)
                              tataGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAAtt                                                                                                                       <  1:800675/148‑1 (MQ=255)
                               ataGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGTTTCTCGTTTCAACTCAATAAGTTATGAAttt                                                                                                                      >  2:2373118/1‑148 (MQ=255)
                               ataGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAAttt                                                                                                                      >  1:1063846/1‑148 (MQ=255)
                                taGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTa                                                                                                                     <  2:3429596/148‑1 (MQ=255)
                                  gCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTTTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGc                                                                                                                   <  1:19411/148‑1 (MQ=255)
                                  gCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGc                                                                                                                   <  1:20431/148‑1 (MQ=255)
                                        tCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGc                                                                                                             >  2:1356343/1‑148 (MQ=255)
                                         cAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCt                                                                                                            <  1:1790703/148‑1 (MQ=255)
                                             gCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGTTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGt                                                                                                        <  1:1628461/148‑1 (MQ=255)
                                               gATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGttt                                                                                                      <  2:1114987/148‑1 (MQ=255)
                                                aTGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTa                                                                                                     <  1:1223896/148‑1 (MQ=255)
                                                   aaCTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAgtg                                                                                                  >  1:1742333/1‑148 (MQ=255)
                                                    aCTTGTCGGAAGTTGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTTTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAgtgt                                                                                                 <  1:2999190/148‑1 (MQ=255)
                                                    aCTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAgtgt                                                                                                 >  1:311640/1‑148 (MQ=255)
                                                    aCTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAgtgt                                                                                                 <  1:925895/148‑1 (MQ=255)
                                                       tGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTAGGTTTAGTGTAtt                                                                                              >  2:1759601/1‑148 (MQ=255)
                                                              aGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTtaata                                                                                       <  2:2088011/148‑1 (MQ=255)
                                                              aGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTtaata                                                                                       <  2:662021/148‑1 (MQ=255)
                                                                aTGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCTAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTaataat                                                                                     >  2:1690920/1‑148 (MQ=255)
                                                                aTGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTaataat                                                                                     >  1:3532003/1‑148 (MQ=255)
                                                                 tGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATc                                                                                    >  1:1919885/1‑148 (MQ=255)
                                                                    cGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGa                                                                                 <  1:2086585/148‑1 (MQ=255)
                                                                      tgtTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGTAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACa                                                                               <  2:3016452/148‑1 (MQ=255)
                                                                      tgtTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACa                                                                               <  2:3087289/148‑1 (MQ=255)
                                                                       gtTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACAt                                                                              >  2:885872/1‑148 (MQ=255)
                                                                          ggTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGc                                                                           >  2:1394446/1‑148 (MQ=255)
                                                                              aTGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAg                                                                       >  1:3503846/1‑148 (MQ=255)
                                                                              aTGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAg                                                                       <  2:742110/148‑1 (MQ=255)
                                                                                 cAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCt                                                                    <  1:2474787/148‑1 (MQ=255)
                                                                                   aTTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCtat                                                                  <  1:918154/148‑1 (MQ=255)
                                                                                   aTTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCtat                                                                  <  1:1326870/148‑1 (MQ=255)
                                                                                   aTTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCtat                                                                  <  1:2710590/148‑1 (MQ=255)
                                                                                   aTTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCtat                                                                  <  1:1089552/148‑1 (MQ=255)
                                                                                   aTTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCtat                                                                  <  2:2847521/148‑1 (MQ=255)
                                                                                        aaCAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTAc                                                             <  2:2576930/148‑1 (MQ=255)
                                                                                         aCAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTAcc                                                            <  2:580601/148‑1 (MQ=255)
                                                                                          cAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCCCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACct                                                           <  1:3482534/148‑1 (MQ=255)
                                                                                             cGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCtt                                                        >  2:1519335/1‑148 (MQ=255)
                                                                                             cGTCGGCTGTTAAACCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCtt                                                        >  1:2976693/1‑148 (MQ=255)
                                                                                              gTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTc                                                       <  1:1394606/148‑1 (MQ=255)
                                                                                               tCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGCTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTcc                                                      >  2:1559684/1‑148 (MQ=255)
                                                                                                cGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTccc                                                     <  2:1612501/148‑1 (MQ=255)
                                                                                                cGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTccc                                                     >  1:1376183/1‑148 (MQ=255)
                                                                                                cGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTccc                                                     >  1:2241271/1‑148 (MQ=255)
                                                                                                    tGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGTTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTAt                                                 <  2:267593/148‑1 (MQ=255)
                                                                                                     gTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTAtt                                                <  2:1934858/148‑1 (MQ=255)
                                                                                                      ttAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTAttt                                               <  2:804971/148‑1 (MQ=255)
                                                                                                      ttAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTAttt                                               <  2:2587903/148‑1 (MQ=255)
                                                                                                      ttAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTAttt                                               >  1:1307543/1‑148 (MQ=255)
                                                                                                        aaTCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGt                                             >  1:596948/1‑148 (MQ=255)
                                                                                                          tCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGtta                                           >  1:1845544/1‑148 (MQ=255)
                                                                                                           ccGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGttat                                          <  2:225089/148‑1 (MQ=255)
                                                                                                            cgcgTTCGAAATATCGACGATCTGGAAAAAATTTATAAGTTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGttatt                                         <  2:3376973/148‑1 (MQ=255)
                                                                                                             gcgTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGttattt                                        >  1:256186/1‑148 (MQ=255)
                                                                                                               gTTCGAAATATCGACGATCTGGAAAAAATTTATAAGTTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGttatttat                                      <  2:1069004/148‑1 (MQ=255)
                                                                                                                 tCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTatttattt                                    <  2:246381/148‑1 (MQ=255)
                                                                                                                  cGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTAtttt                                   >  1:1903533/1‑148 (MQ=255)
                                                                                                                   gAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTa                                  <  1:2859720/148‑1 (MQ=255)
                                                                                                                    aaaTATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTaa                                 <  2:2174199/148‑1 (MQ=255)
                                                                                                                    aaaTATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTaa                                 <  1:555778/148‑1 (MQ=255)
                                                                                                                    aaaTATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTCCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTaa                                 <  2:957097/148‑1 (MQ=255)
                                                                                                                       tatCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGt                              >  1:3073117/1‑148 (MQ=255)
                                                                                                                       tatCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGt                              <  2:578839/148‑1 (MQ=255)
                                                                                                                        atCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGtt                             >  2:3557481/1‑148 (MQ=255)
                                                                                                                         tCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGttt                            <  2:1749815/148‑1 (MQ=255)
                                                                                                                         tCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGttt                            <  2:1748327/148‑1 (MQ=255)
                                                                                                                           gacgaTCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCa                          <  1:3067059/148‑1 (MQ=255)
                                                                                                                           gacgaTCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCa                          <  2:1798849/148‑1 (MQ=255)
                                                                                                                           gacgaTCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCa                          <  2:145633/148‑1 (MQ=255)
                                                                                                                            acgaTCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCAt                         >  2:690205/1‑148 (MQ=255)
                                                                                                                            acgaTCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCAt                         >  2:1529793/1‑148 (MQ=255)
                                                                                                                             cgaTCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCAtt                        >  2:345778/1‑148 (MQ=255)
                                                                                                                                 cTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGata                    <  2:3503996/148‑1 (MQ=255)
                                                                                                                                   ggAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGatata                  >  1:870088/1‑148 (MQ=255)
                                                                                                                                       aaaaTTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATaaa              <  1:1613143/148‑1 (MQ=255)
                                                                                                                                        aaaTTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAAt             <  1:1541519/148‑1 (MQ=255)
                                                                                                                                             tataAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCaaa        <  1:3050823/148‑1 (MQ=255)
                                                                                                                                              ataAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAAt       >  2:1960636/1‑148 (MQ=255)
                                                                                                                                               taAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATg      <  2:1845544/148‑1 (MQ=255)
                                                                                                                                                aaGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGa     >  1:1007189/1‑148 (MQ=255)
                                                                                                                                                aaGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGa     <  2:200866/148‑1 (MQ=255)
                                                                                                                                                  gCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGaaa   <  1:2611687/148‑1 (MQ=255)
                                                                                                                                                   cTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGaaaa  >  2:61334/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GGTTTTTTCTTCGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTCGGAAGATGGCGTGTTGGTGATGCAATTAGAACAGCGTCGGCTGTTAATCCGCGTTCGAAATATCGACGATCTGGAAAAAATTTATAAGCTTCTCGTTTCAACTCAATAAGTTATGAATTTAGCCAAAGCTATGTTTAGTGTATTTTTAATAATCAGACATAGCTTAGGCTATATTACCTCTTCCCTTATTTGTTATTTATTTTAACGTTTCATTGATATATAAATCCAAATGAAAA  >  CP009273/1899349‑1899643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑