Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 173,725 G→T 100% G129G (GGC→GGA)  yadS ← UPF0126 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273173,7250GT96.5% 606.1 / 11.9 198G129G (GGC→GGA) yadSUPF0126 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (4/3);  new base T (97/94);  total (101/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 205 total aligned reads displayed.

TCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCGCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGC  >  CP009273/173580‑173869
                                                                                                                                                 |                                                                                                                                                
tcatACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCgcctcc                                                                                                                                                >  1:1620509/1‑148 (MQ=255)
   tACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAAc                                                                                                                                             >  2:1160044/1‑148 (MQ=255)
        gCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAg                                                                                                                                        >  2:665068/1‑148 (MQ=255)
          tGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTa                                                                                                                                      <  2:1022854/148‑1 (MQ=255)
           gTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTaa                                                                                                                                     >  2:2017944/1‑148 (MQ=255)
           gTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTaa                                                                                                                                     >  2:1225391/1‑148 (MQ=255)
           gTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTaa                                                                                                                                     >  1:3228301/1‑148 (MQ=255)
            tttCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAAt                                                                                                                                    >  2:538901/1‑148 (MQ=255)
            tttCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAAt                                                                                                                                    >  2:3129259/1‑148 (MQ=255)
            tttCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAAt                                                                                                                                    >  1:1794906/1‑148 (MQ=255)
              tCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGa                                                                                                                                  <  2:758982/148‑1 (MQ=255)
                 aGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGc                                                                                                                               <  1:1596157/148‑1 (MQ=255)
                   tGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGccc                                                                                                                             <  2:2353232/148‑1 (MQ=255)
                   tGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGccc                                                                                                                             <  2:262494/148‑1 (MQ=255)
                   tGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGccc                                                                                                                             <  2:1300292/148‑1 (MQ=255)
                   tGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGccc                                                                                                                             >  2:1011988/1‑148 (MQ=255)
                         ggAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACaa                                                                                                                       >  1:1724875/1‑148 (MQ=255)
                         ggAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACaa                                                                                                                       >  1:3480625/1‑148 (MQ=255)
                            aaaTGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAAcc                                                                                                                    <  2:3120742/148‑1 (MQ=255)
                               tgtgTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACcgcg                                                                                                                 >  1:1462486/1‑148 (MQ=255)
                                   taataaGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTa                                                                                                             <  2:677862/148‑1 (MQ=255)
                                   taataaGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTa                                                                                                             <  2:1719136/148‑1 (MQ=255)
                                      taaGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACg                                                                                                          <  1:1160405/148‑1 (MQ=255)
                                       aaGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACgg                                                                                                         >  1:2503409/1‑148 (MQ=255)
                                          ccGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGAcc                                                                                                      >  1:3073476/1‑148 (MQ=255)
                                           cGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCg                                                                                                     <  1:1369886/148‑1 (MQ=255)
                                           cGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCg                                                                                                     <  1:2527777/148‑1 (MQ=255)
                                             tAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGc                                                                                                   <  2:1273429/148‑1 (MQ=255)
                                             tAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGc                                                                                                   <  2:3375124/148‑1 (MQ=255)
                                               gCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCtt                                                                                                 <  2:2353151/148‑1 (MQ=255)
                                                cGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTc                                                                                                >  2:1400562/1‑148 (MQ=255)
                                                 gTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTcc                                                                                               <  1:3394111/148‑1 (MQ=255)
                                                    gACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCa                                                                                            >  2:311393/1‑148 (MQ=255)
                                                    gACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCa                                                                                            >  2:311226/1‑148 (MQ=255)
                                                       aaTACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTa                                                                                         >  1:2618396/1‑148 (MQ=255)
                                                         tACCGCCTATAATACAGGCAGTTGCGTATATTTCTGTACGTAAAATCATGGGGATTTGGCGGGCCTGAACATCACGAATGATCCCGCCTCAAACGCCAGTAATGACGCCCTTACAAACCGCGATTAACGGACCTGCTTCCGCATTaaa                                                                                       <  1:2412983/148‑1 (MQ=255)
                                                         tACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTaaa                                                                                       >  1:2280230/1‑148 (MQ=255)
                                                          aCCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAg                                                                                      >  1:407440/1‑148 (MQ=255)
                                                            cgccgATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGc                                                                                    <  2:2467220/148‑1 (MQ=255)
                                                             gccgATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCt                                                                                   >  1:762297/1‑148 (MQ=255)
                                                             gccgATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCt                                                                                   <  1:2874127/148‑1 (MQ=255)
                                                             gccgATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCt                                                                                   <  1:2873422/148‑1 (MQ=255)
                                                             gccgATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCAGTAAAGGCt                                                                                   >  1:1661576/1‑148 (MQ=255)
                                                                gATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTa                                                                                >  1:46877/1‑148 (MQ=255)
                                                                 ataataCAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTAt                                                                               >  1:3528367/1‑148 (MQ=255)
                                                                 ataataCAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTAt                                                                               >  1:604294/1‑148 (MQ=255)
                                                                   aataCAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCTACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTc                                                                             <  2:247535/148‑1 (MQ=255)
                                                                   aataCAGGCAGGTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTc                                                                             <  2:3228679/148‑1 (MQ=255)
                                                                       cAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGc                                                                         <  2:2145856/148‑1 (MQ=255)
                                                                        aGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGcc                                                                        >  2:2136602/1‑148 (MQ=255)
                                                                         ggCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCGCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCa                                                                       <  1:111741/148‑1 (MQ=255)
                                                                          gCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCaa                                                                      <  1:1087472/148‑1 (MQ=255)
                                                                          gCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCaa                                                                      <  1:318241/148‑1 (MQ=255)
                                                                            aGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATg                                                                    <  2:2382729/148‑1 (MQ=255)
                                                                              ttGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGcc                                                                  >  2:2828568/1‑148 (MQ=255)
                                                                                   tAGTTTTCTGTTCGTAAAATCTTGGTGATTTCGAGGGCCAGAACATCTCGTTTGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGAGCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACaa                                                             <  1:86132/148‑1 (MQ=255)
                                                                                     gATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAac                                                           <  2:369191/148‑1 (MQ=255)
                                                                                     gATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAac                                                           <  2:2160779/148‑1 (MQ=255)
                                                                                      aTTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAaca                                                          <  1:1842751/148‑1 (MQ=255)
                                                                                      aTTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAaca                                                          >  1:882462/1‑148 (MQ=255)
                                                                                      aTTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAaca                                                          <  2:3429086/148‑1 (MQ=255)
                                                                                      aTTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAaca                                                          >  1:255985/1‑148 (MQ=255)
                                                                                      aTTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCGCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAaca                                                          >  1:1456531/1‑148 (MQ=255)
                                                                                             tACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAg                                                   <  1:825391/148‑1 (MQ=255)
                                                                                              aCGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGa                                                  <  1:148107/148‑1 (MQ=255)
                                                                                                 tAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCa                                               >  2:1674583/1‑148 (MQ=255)
                                                                                                 tAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCa                                               >  2:300917/1‑148 (MQ=255)
                                                                                                    aaTCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACg                                            >  1:3330058/1‑148 (MQ=255)
                                                                                                       cATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCg                                         >  1:474702/1‑148 (MQ=255)
                                                                                                        aTGTGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGt                                        >  1:3464969/1‑148 (MQ=255)
                                                                                                         tGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTc                                       <  1:2899116/148‑1 (MQ=255)
                                                                                                            ggATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAg                                    >  1:36375/1‑148 (MQ=255)
                                                                                                            ggATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAg                                    >  1:794245/1‑148 (MQ=255)
                                                                                                            ggATTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAg                                    >  2:3266358/1‑148 (MQ=255)
                                                                                                              aTTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCa                                  >  2:1445346/1‑148 (MQ=255)
                                                                                                              aTTTCGCGGGCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCa                                  <  1:296229/148‑1 (MQ=255)
                                                                                                                     gggCCAGAACATCACGAATGATCCCGCCTCCAACGCCATTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCaa                           <  1:1026078/148‑1 (MQ=255)
                                                                                                                      ggCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAAc                          <  1:1011784/148‑1 (MQ=255)
                                                                                                                      ggCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAAc                          <  2:1868179/148‑1 (MQ=255)
                                                                                                                      ggCCAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAAc                          <  2:3480625/148‑1 (MQ=255)
                                                                                                                        ccAGAACATCACGAATGATCCCTCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACAt                        >  1:2327715/1‑148 (MQ=255)
                                                                                                                         cAGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATc                       >  1:2058885/1‑148 (MQ=255)
                                                                                                                          aGAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATcc                      <  2:2798389/148‑1 (MQ=255)
                                                                                                                           gAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCa                     >  1:2749973/1‑148 (MQ=255)
                                                                                                                           gAACATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCa                     >  2:2268023/1‑148 (MQ=255)
                                                                                                                            aaCATCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCAt                    >  1:1538976/1‑148 (MQ=255)
                                                                                                                               aTCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCAtttt                 <  1:1642523/148‑1 (MQ=255)
                                                                                                                                tCACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTg                >  1:2497115/1‑148 (MQ=255)
                                                                                                                                 cACGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTgg               <  1:1787012/148‑1 (MQ=255)
                                                                                                                                  aCGAATGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGt              <  1:2136610/148‑1 (MQ=255)
                                                                                                                                       tGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCg         <  2:407440/148‑1 (MQ=255)
                                                                                                                                       tGATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCg         >  2:1940671/1‑148 (MQ=255)
                                                                                                                                        gATCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGt        >  1:224086/1‑148 (MQ=255)
                                                                                                                                         aTCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTc       <  1:2017944/148‑1 (MQ=255)
                                                                                                                                         aTCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTc       <  1:2612424/148‑1 (MQ=255)
                                                                                                                                          tCCCGCCTCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCt      >  2:2560027/1‑148 (MQ=255)
                                                                                                                                            ccgcctCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTtg    <  2:1772265/148‑1 (MQ=255)
                                                                                                                                            ccgcctCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTtg    <  1:3129259/148‑1 (MQ=255)
                                                                                                                                              gcctccAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTtggc  <  1:494923/148‑1 (MQ=255)
                                                                                                                                              gcctccAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTtggc  <  1:1225391/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCGCCAACGCCAGTAATGACGCCCATACAAACCGCGATTAACGGACCGGCTTCCGCATTAAAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGC  >  CP009273/173580‑173869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑