Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,953,876 C→A 100% P76P (CCG→CCT)  yecM ← putative metal‑binding enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,953,8760CA96.0% 609.9 / 14.9 200P76P (CCG→CCT) yecMputative metal‑binding enzyme
Reads supporting (aligned to +/- strand):  ref base C (5/3);  new base A (104/88);  total (109/91)
Fisher's exact test for biased strand distribution p-value = 7.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 200 total aligned reads displayed.

GCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGATATTCAACCCCAGACGGCGG  >  CP009273/1953731‑1954020
                                                                                                                                                 |                                                                                                                                                
gCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAgg                                                                                                                                                <  1:2390690/148‑1 (MQ=255)
 cGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGt                                                                                                                                               >  2:3269129/1‑148 (MQ=255)
 cGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGt                                                                                                                                               <  1:3301690/148‑1 (MQ=255)
   tttCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCt                                                                                                                                             <  2:2465136/148‑1 (MQ=255)
      cTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGcc                                                                                                                                          <  1:1402073/148‑1 (MQ=255)
      cTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGcc                                                                                                                                          <  2:3072100/148‑1 (MQ=255)
        ggATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCAt                                                                                                                                        >  2:3339242/1‑148 (MQ=255)
           tCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGa                                                                                                                                     <  2:2972044/148‑1 (MQ=255)
             aCCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATc                                                                                                                                   <  2:3094762/148‑1 (MQ=255)
               ccGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCat                                                                                                                                 <  2:1416049/148‑1 (MQ=255)
               ccGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCat                                                                                                                                 <  2:2356946/148‑1 (MQ=255)
               ccGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCat                                                                                                                                 >  1:331919/1‑148 (MQ=255)
               ccGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCat                                                                                                                                 <  2:58791/148‑1 (MQ=255)
                cGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCata                                                                                                                                <  2:3177669/148‑1 (MQ=255)
                  gCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATAtt                                                                                                                              >  2:878162/1‑148 (MQ=255)
                    aGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATACAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATAtttt                                                                                                                            <  2:3317268/148‑1 (MQ=255)
                     gAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTc                                                                                                                           >  1:876225/1‑148 (MQ=255)
                        cGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGa                                                                                                                        >  1:1622812/1‑148 (MQ=255)
                          aTTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACa                                                                                                                      >  1:3144460/1‑148 (MQ=255)
                           tttCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACaa                                                                                                                     <  2:1772583/148‑1 (MQ=255)
                            ttCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACaaa                                                                                                                    <  1:2085371/148‑1 (MQ=255)
                                atatGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTGAAACAGACAAATAGGTCTGCCATTGTTCATATTTTCTGACAAAAGCt                                                                                                                >  2:1359082/1‑148 (MQ=255)
                                 tatGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTc                                                                                                               >  1:2831074/1‑148 (MQ=255)
                                  atGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCg                                                                                                              >  1:3294308/1‑148 (MQ=255)
                                  atGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCg                                                                                                              >  2:1423785/1‑148 (MQ=255)
                                  atGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCg                                                                                                              >  1:789505/1‑148 (MQ=255)
                                  atGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCg                                                                                                              >  1:3087780/1‑148 (MQ=255)
                                    gTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGcc                                                                                                            <  1:996125/148‑1 (MQ=255)
                                       cccAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCcaca                                                                                                         <  2:2374706/148‑1 (MQ=255)
                                       cccAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCcaca                                                                                                         >  2:2912879/1‑148 (MQ=255)
                                         cAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACt                                                                                                       >  2:1194859/1‑148 (MQ=255)
                                         cAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACt                                                                                                       >  1:1132972/1‑148 (MQ=255)
                                          aaCCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTg                                                                                                      >  1:1730360/1‑148 (MQ=255)
                                             cTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTc                                                                                                   >  1:2432339/1‑148 (MQ=255)
                                               tCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCaa                                                                                                 <  2:865650/148‑1 (MQ=255)
                                               tCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCaa                                                                                                 >  2:2889753/1‑148 (MQ=255)
                                                cGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCaaa                                                                                                >  2:1788452/1‑148 (MQ=255)
                                                 gtgtGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAAc                                                                                               <  1:3162969/148‑1 (MQ=255)
                                                  tgtgGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAAcc                                                                                              <  1:1327690/148‑1 (MQ=255)
                                                      ggTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCcgcg                                                                                          >  1:3411234/1‑148 (MQ=255)
                                                          aCGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGc                                                                                      <  2:1965996/148‑1 (MQ=255)
                                                            gTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCa                                                                                    <  1:3044451/148‑1 (MQ=255)
                                                              ttttCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGc                                                                                  >  1:898070/1‑148 (MQ=255)
                                                              ttttCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGc                                                                                  >  1:1230350/1‑148 (MQ=255)
                                                                 tCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGtt                                                                               <  1:1447773/148‑1 (MQ=255)
                                                                 tCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGtt                                                                               <  1:478335/148‑1 (MQ=255)
                                                                  ccccGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTc                                                                              <  1:467337/148‑1 (MQ=255)
                                                                    ccGGGCCACGTTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTcag                                                                            <  2:1254034/148‑1 (MQ=255)
                                                                     cGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTcagc                                                                           >  2:3264502/1‑148 (MQ=255)
                                                                      gggCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTcagca                                                                          <  2:633787/148‑1 (MQ=255)
                                                                              gTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAACTAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGtt                                                                  <  2:824128/148‑1 (MQ=255)
                                                                               tAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGttt                                                                 <  2:1145912/148‑1 (MQ=255)
                                                                                  cTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGa                                                              >  1:3265678/1‑148 (MQ=255)
                                                                                  cTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGa                                                              >  1:1475249/1‑148 (MQ=255)
                                                                                   tCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGAt                                                             <  2:1524015/148‑1 (MQ=255)
                                                                                   tCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGAt                                                             <  2:1605493/148‑1 (MQ=255)
                                                                                    ccACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATg                                                            >  2:1244444/1‑148 (MQ=255)
                                                                                     cacaATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATgg                                                           >  1:546153/1‑148 (MQ=255)
                                                                                       caATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCa                                                         <  2:2521531/148‑1 (MQ=255)
                                                                                       caATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCa                                                         <  2:2227980/148‑1 (MQ=255)
                                                                                        aaTAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCaa                                                        >  2:134782/1‑148 (MQ=255)
                                                                                         aTAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAAc                                                       >  2:266684/1‑148 (MQ=255)
                                                                                         aTAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAAc                                                       >  2:1782143/1‑148 (MQ=255)
                                                                                          tAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACg                                                      >  2:751324/1‑148 (MQ=255)
                                                                                           aGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGc                                                     >  1:1295672/1‑148 (MQ=255)
                                                                                           aGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGc                                                     >  2:528519/1‑148 (MQ=255)
                                                                                               aaCTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAg                                                 >  1:2216220/1‑148 (MQ=255)
                                                                                                aCTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGa                                                >  1:2542789/1‑148 (MQ=255)
                                                                                                aCTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGa                                                >  1:863001/1‑148 (MQ=255)
                                                                                                  tGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGaaa                                              <  1:3527531/148‑1 (MQ=255)
                                                                                                    ccAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAAtg                                            <  2:1733967/148‑1 (MQ=255)
                                                                                                      aGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAAtgtg                                          >  2:2952898/1‑148 (MQ=255)
                                                                                                        tGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGAt                                        >  1:1784399/1‑148 (MQ=255)
                                                                                                        tGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGAt                                        >  2:1909913/1‑148 (MQ=255)
                                                                                                             aaCCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCa                                   <  2:1174159/148‑1 (MQ=255)
                                                                                                                 tGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCa                               >  1:417066/1‑148 (MQ=255)
                                                                                                                  gTACCGGTTCATGCAATTTAAACCGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAg                              <  1:1870955/148‑1 (MQ=255)
                                                                                                                   tACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGc                             >  2:578409/1‑148 (MQ=255)
                                                                                                                   tACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTGTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGc                             >  1:1287178/1‑148 (MQ=255)
                                                                                                                    aCCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCg                            >  2:657307/1‑148 (MQ=255)
                                                                                                                     ccGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCgg                           <  1:3391401/148‑1 (MQ=255)
                                                                                                                      cGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTTATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGATCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGa                          >  2:3353756/1‑148 (MQ=255)
                                                                                                                       ggTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAg                         >  2:1797657/1‑148 (MQ=255)
                                                                                                                        gTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGt                        >  1:2886194/1‑148 (MQ=255)
                                                                                                                         ttCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTg                       >  1:2047453/1‑148 (MQ=255)
                                                                                                                         ttCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTg                       >  1:1242806/1‑148 (MQ=255)
                                                                                                                          tCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGa                      >  1:3485361/1‑148 (MQ=255)
                                                                                                                             tGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGatat                   <  1:1013220/148‑1 (MQ=255)
                                                                                                                              gCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGATAtt                  >  1:2679892/1‑148 (MQ=255)
                                                                                                                               cAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGATATTc                 >  2:1539146/1‑148 (MQ=255)
                                                                                                                                   ttAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGATATTCAAcc             >  2:2074181/1‑148 (MQ=255)
                                                                                                                                      aaCAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGATATTCAACCCCa          >  1:1596034/1‑148 (MQ=255)
                                                                                                                                      aaCAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGATATTCAACCCCa          >  1:168067/1‑148 (MQ=255)
                                                                                                                                      aaCAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGAGATATTCAACCCCa          >  1:170313/1‑148 (MQ=255)
                                                                                                                                       aCAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGATATTCAACCCCAg         >  2:3153042/1‑148 (MQ=255)
                                                                                                                                        cAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGATATTCAACCCCAGa        <  2:949035/148‑1 (MQ=255)
                                                                                                                                            cAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGATATTCAACCCCAGAcggc    >  2:2564100/1‑148 (MQ=255)
                                                                                                                                             aaaTAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGATATTCAACCCCAGAcggcg   >  2:1070106/1‑148 (MQ=255)
                                                                                                                                             aaaTAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGATATTCAACCCCAGAcggcg   <  2:2292862/148‑1 (MQ=255)
                                                                                                                                              aaTAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGATATTCAACCCCAGAcggcgg  >  2:2415419/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGATATTCAACCCCAGACGGCGG  >  CP009273/1953731‑1954020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑