Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,962,923 C→A 100% M233I (ATG→ATT)  cheR ← chemotaxis regulator, protein‑glutamate methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,962,9230CA95.0% 524.9 / 17.2 181M233I (ATG→ATT) cheRchemotaxis regulator, protein‑glutamate methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (6/3);  new base A (75/97);  total (81/100)
Fisher's exact test for biased strand distribution p-value = 3.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 181 total aligned reads displayed.

CATACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAA  >  CP009273/1962778‑1963069
                                                                                                                                                 |                                                                                                                                                  
cATACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAAt                                                                                                                                                  <  2:369162/148‑1 (MQ=255)
 aTACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATg                                                                                                                                                 <  2:241804/148‑1 (MQ=255)
 aTACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATg                                                                                                                                                 <  2:243137/148‑1 (MQ=255)
   acacCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGAc                                                                                                                                               >  1:3091056/1‑148 (MQ=255)
    cacCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACg                                                                                                                                              <  1:209926/148‑1 (MQ=255)
    cacCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACg                                                                                                                                              <  1:210297/148‑1 (MQ=255)
    cacCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACg                                                                                                                                              >  2:3091521/1‑148 (MQ=255)
     acCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGt                                                                                                                                             <  2:689144/148‑1 (MQ=255)
     acCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGt                                                                                                                                             <  2:1644421/148‑1 (MQ=255)
      ccGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGATAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTTAGGAGCGGAACACAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGtt                                                                                                                                            <  2:1432419/148‑1 (MQ=255)
       cGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTa                                                                                                                                           <  1:1364480/148‑1 (MQ=255)
       cGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTa                                                                                                                                           <  1:824332/148‑1 (MQ=255)
       cGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTa                                                                                                                                           >  1:1985736/1‑148 (MQ=255)
           tGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGAc                                                                                                                                       >  1:518310/1‑148 (MQ=255)
            gACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACa                                                                                                                                      >  2:701046/1‑148 (MQ=255)
              ccACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACaga                                                                                                                                    >  2:1826665/1‑148 (MQ=255)
                 cGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACagaaga                                                                                                                                 >  1:2993158/1‑148 (MQ=255)
                 cGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACagaaga                                                                                                                                 <  2:1226647/148‑1 (MQ=255)
                 cGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACagaaga                                                                                                                                 <  2:1226541/148‑1 (MQ=255)
                 cGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACagaaga                                                                                                                                 >  2:2085874/1‑148 (MQ=255)
                 cGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACagaaga                                                                                                                                 >  2:2876438/1‑148 (MQ=255)
                  gCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGAt                                                                                                                                <  2:725437/148‑1 (MQ=255)
                   cAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATc                                                                                                                               >  1:40045/1‑148 (MQ=255)
                    aGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCg                                                                                                                              <  1:388222/148‑1 (MQ=255)
                      cGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCa                                                                                                                            <  1:518588/148‑1 (MQ=255)
                      cGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCa                                                                                                                            >  2:135681/1‑148 (MQ=255)
                        tGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATc                                                                                                                          >  2:1572450/1‑148 (MQ=255)
                         gAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCa                                                                                                                         <  1:1431709/148‑1 (MQ=255)
                               gcgcTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGc                                                                                                                   <  2:3380495/148‑1 (MQ=255)
                                cgcTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAACGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGcc                                                                                                                  <  2:2608510/148‑1 (MQ=255)
                                        gTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACg                                                                                                          <  1:813365/148‑1 (MQ=255)
                                         tGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACgg                                                                                                         <  2:3487532/148‑1 (MQ=255)
                                             tAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTa                                                                                                     >  1:2990079/1‑148 (MQ=255)
                                             tAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATTACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTa                                                                                                     >  2:2861010/1‑148 (MQ=255)
                                              aaaGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTAc                                                                                                    >  1:3027488/1‑148 (MQ=255)
                                              aaaGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTAc                                                                                                    <  1:3310538/148‑1 (MQ=255)
                                              aaaGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTAc                                                                                                    >  1:1620835/1‑148 (MQ=255)
                                                 gTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGt                                                                                                 >  2:2131080/1‑148 (MQ=255)
                                                 gTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGt                                                                                                 >  1:496531/1‑148 (MQ=255)
                                                   tttCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGttt                                                                                               <  2:2265134/148‑1 (MQ=255)
                                                        gagTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCa                                                                                          >  1:1048347/1‑148 (MQ=255)
                                                          gTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGt                                                                                        <  2:2083711/148‑1 (MQ=255)
                                                           tGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTa                                                                                       >  2:3435184/1‑148 (MQ=255)
                                                           tGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTa                                                                                       >  2:929048/1‑148 (MQ=255)
                                                           tGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTa                                                                                       >  2:2707140/1‑148 (MQ=255)
                                                            gACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAg                                                                                      <  2:2223589/148‑1 (MQ=255)
                                                             aCCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGa                                                                                     >  2:1971156/1‑148 (MQ=255)
                                                               ccGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGAtt                                                                                   >  1:1054387/1‑148 (MQ=255)
                                                                cGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTc                                                                                  <  2:2981888/148‑1 (MQ=255)
                                                                   aaaCAGCAATCCGTCGGGATTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTTATGTGTAGTTTGATCGAAGTAGATAATCACGTTACGACAGAAGATCGCATCAAACGGCACCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGc                                                                               >  1:1613586/1‑148 (MQ=255)
                                                                    aaCAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCg                                                                              <  1:1194535/148‑1 (MQ=255)
                                                                    aaCAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCg                                                                              >  1:1699024/1‑148 (MQ=255)
                                                                     aCAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGCCGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCgg                                                                             <  1:1368635/148‑1 (MQ=255)
                                                                     aCAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCgg                                                                             >  1:2787948/1‑148 (MQ=255)
                                                                       aGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGGTACGACAGAAGATCGCATCAAACTGCCACGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCgggg                                                                           >  1:1138704/1‑148 (MQ=255)
                                                                        gCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGc                                                                          >  2:336882/1‑148 (MQ=255)
                                                                         cAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCa                                                                         >  2:469503/1‑148 (MQ=255)
                                                                         cAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCa                                                                         >  2:469472/1‑148 (MQ=255)
                                                                           aTCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCaaa                                                                       <  1:3126854/148‑1 (MQ=255)
                                                                           aTCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCaaa                                                                       <  1:1359733/148‑1 (MQ=255)
                                                                            tCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCaaaa                                                                      >  1:2585644/1‑148 (MQ=255)
                                                                            tCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCaaaa                                                                      >  1:3293397/1‑148 (MQ=255)
                                                                                 cGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAAc                                                                 <  2:1614972/148‑1 (MQ=255)
                                                                                 cGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAAc                                                                 <  1:2941917/148‑1 (MQ=255)
                                                                                  gggTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACa                                                                <  2:3429057/148‑1 (MQ=255)
                                                                                   ggTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACAt                                                               >  2:903551/1‑148 (MQ=255)
                                                                                   ggTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACAt                                                               >  2:1873880/1‑148 (MQ=255)
                                                                                    gTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATa                                                              <  2:578206/148‑1 (MQ=255)
                                                                                    gTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATa                                                              <  2:2839063/148‑1 (MQ=255)
                                                                                      ttAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGt                                                            <  2:94429/148‑1 (MQ=255)
                                                                                       tAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGtt                                                           <  1:1792282/148‑1 (MQ=255)
                                                                                       tAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGtt                                                           >  2:1687522/1‑148 (MQ=255)
                                                                                         aGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCg                                                         >  1:2472584/1‑148 (MQ=255)
                                                                                           gAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGcc                                                       >  2:2784090/1‑148 (MQ=255)
                                                                                                gAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTc                                                  <  2:2168640/148‑1 (MQ=255)
                                                                                                gAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTc                                                  <  1:135681/148‑1 (MQ=255)
                                                                                                gAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTc                                                  <  2:1258053/148‑1 (MQ=255)
                                                                                                 aaCAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTcc                                                 <  2:2095243/148‑1 (MQ=255)
                                                                                                 aaCAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTcc                                                 >  1:1959568/1‑148 (MQ=255)
                                                                                                 aaCAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTcc                                                 >  1:431495/1‑148 (MQ=255)
                                                                                                       gcggcgCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGacgc                                           >  2:543775/1‑148 (MQ=255)
                                                                                                        cggcgCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGacgca                                          >  1:864391/1‑148 (MQ=255)
                                                                                                             cAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGt                                     >  1:3355204/1‑148 (MQ=255)
                                                                                                             cAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGt                                     >  1:2684999/1‑148 (MQ=255)
                                                                                                              aaaaTCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTa                                    <  2:1026033/148‑1 (MQ=255)
                                                                                                                aaTCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAATAACGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGAAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCATGACGCACGCGTAcc                                  >  1:3304294/1‑148 (MQ=255)
                                                                                                                aaTCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCACACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTAcc                                  <  1:26794/148‑1 (MQ=255)
                                                                                                                aaTCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTAcc                                  <  1:1826665/148‑1 (MQ=255)
                                                                                                                     gctgctgGGTATTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGccc                             <  2:1729854/147‑1 (MQ=255)
                                                                                                                      ctgctgGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCt                            <  2:518302/148‑1 (MQ=255)
                                                                                                                              tAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCgg                    <  1:701046/148‑1 (MQ=255)
                                                                                                                                gTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGcc                  <  1:2808363/148‑1 (MQ=255)
                                                                                                                                gTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGcc                  <  1:2645889/148‑1 (MQ=255)
                                                                                                                                   tGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCg               <  1:210847/148‑1 (MQ=255)
                                                                                                                                      tCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTcc            <  2:1867749/148‑1 (MQ=255)
                                                                                                                                      tCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTcc            >  1:2031124/1‑148 (MQ=255)
                                                                                                                                       cGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTccc           <  1:2317226/148‑1 (MQ=255)
                                                                                                                                         aaGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTc         >  1:1619370/1‑148 (MQ=255)
                                                                                                                                           gTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGc       <  2:1059171/148‑1 (MQ=255)
                                                                                                                                           gTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGc       <  1:2707140/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CATACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAA  >  CP009273/1962778‑1963069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑