Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,986,289 T→A 100% N4Y (AAT→TAT)  pgsA ← phosphatidylglycerophosphate synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,986,2890TA99.5% 603.5 / ‑4.6 181N4Y (AAT→TAT) pgsAphosphatidylglycerophosphate synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (100/80);  total (100/81)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 184 total aligned reads displayed.

AGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGACGCT  >  CP009273/1986144‑1986432
                                                                                                                                                 |                                                                                                                                               
 gAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATaaaa                                                                                                                                              <  2:2815385/148‑1 (MQ=255)
 gAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTaaa                                                                                                                                              <  2:3374146/148‑1 (MQ=255)
      ccATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGCATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCa                                                                                                                                         <  2:323658/148‑1 (MQ=255)
      ccATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCa                                                                                                                                         <  2:1993427/148‑1 (MQ=255)
       cATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCAt                                                                                                                                        >  2:2491118/1‑148 (MQ=255)
       cATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCAt                                                                                                                                        >  1:3027296/1‑148 (MQ=255)
        aTCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATa                                                                                                                                       <  1:605775/148‑1 (MQ=255)
         tCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATaa                                                                                                                                      <  2:2243443/148‑1 (MQ=255)
         tCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATaa                                                                                                                                      <  2:2724386/148‑1 (MQ=255)
           gAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATg                                                                                                                                    <  1:2017905/148‑1 (MQ=255)
             aCCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGAc                                                                                                                                  <  2:1445662/148‑1 (MQ=255)
                 gtcagtcaCCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGt                                                                                                                              >  1:2692818/1‑148 (MQ=255)
                     gtcaCCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACt                                                                                                                          >  2:895787/1‑148 (MQ=255)
                     gtcaCCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACt                                                                                                                          >  1:2547877/1‑148 (MQ=255)
                       caCCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTAt                                                                                                                        <  2:878452/148‑1 (MQ=255)
                       caCCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTAt                                                                                                                        <  2:230644/148‑1 (MQ=255)
                          cgccgcGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCtg                                                                                                                     >  2:1371359/1‑148 (MQ=255)
                            ccgcGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCtgtt                                                                                                                   <  2:2300122/148‑1 (MQ=255)
                               cgACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTc                                                                                                                <  1:260402/148‑1 (MQ=255)
                               cgACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTc                                                                                                                >  2:1474340/1‑148 (MQ=255)
                               cgACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTc                                                                                                                >  2:312807/1‑148 (MQ=255)
                                    cAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTaa                                                                                                           <  1:2544767/148‑1 (MQ=255)
                                       aaaaTGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGAt                                                                                                        >  2:3301923/1‑148 (MQ=255)
                                          aTGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTAc                                                                                                     >  2:1149775/1‑148 (MQ=255)
                                               cgcgGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTa                                                                                                <  2:716708/148‑1 (MQ=255)
                                                gcggcgGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTAt                                                                                               <  2:458765/148‑1 (MQ=255)
                                                gcggcgGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTAt                                                                                               <  2:458794/148‑1 (MQ=255)
                                                 cggcggCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATg                                                                                              <  1:2944591/148‑1 (MQ=255)
                                                  ggcggcAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGt                                                                                             <  2:1737310/148‑1 (MQ=255)
                                                  ggcggcAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGt                                                                                             <  1:2614270/148‑1 (MQ=255)
                                                      gcAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCt                                                                                         >  2:2485048/1‑148 (MQ=255)
                                                          aCGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACag                                                                                     <  2:2660926/148‑1 (MQ=255)
                                                          aCGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACag                                                                                     <  1:657417/148‑1 (MQ=255)
                                                          aCGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACag                                                                                     >  2:2734265/1‑148 (MQ=255)
                                                          aCGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACag                                                                                     >  1:3500025/1‑148 (MQ=255)
                                                             gCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAgaga                                                                                  >  1:1821376/1‑148 (MQ=255)
                                                                aCCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACAt                                                                               <  1:3097323/148‑1 (MQ=255)
                                                                 ccAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATc                                                                              <  1:60633/148‑1 (MQ=255)
                                                                 ccAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATc                                                                              >  1:917178/1‑148 (MQ=255)
                                                                   aGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATTATGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCaa                                                                            <  2:3114269/148‑1 (MQ=255)
                                                                    ggTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAAt                                                                           >  2:127834/1‑148 (MQ=255)
                                                                    ggTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAAt                                                                           <  1:2505194/148‑1 (MQ=255)
                                                                     gTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATg                                                                          >  1:38301/1‑148 (MQ=255)
                                                                       gACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGtt                                                                        >  1:3315043/1‑148 (MQ=255)
                                                                       gACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGtt                                                                        <  1:2221309/148‑1 (MQ=255)
                                                                        aCAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGttt                                                                       >  2:2915783/1‑148 (MQ=255)
                                                                        aCAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGttt                                                                       <  2:2078792/148‑1 (MQ=255)
                                                                        aCAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGttt                                                                       >  1:2351301/1‑148 (MQ=255)
                                                                          aGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCa                                                                     >  1:2159692/1‑148 (MQ=255)
                                                                             cAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACg                                                                  >  2:1252277/1‑148 (MQ=255)
                                                                               gATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGAc                                                                <  2:2501150/148‑1 (MQ=255)
                                                                               gATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGAc                                                                >  1:1555500/1‑148 (MQ=255)
                                                                                aTAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGAcc                                                               >  2:183588/1‑148 (MQ=255)
                                                                                aTAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGAcc                                                               >  2:63765/1‑148 (MQ=255)
                                                                                 tAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCa                                                              >  2:1652528/1‑148 (MQ=255)
                                                                                  aaaaGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCag                                                             >  2:3097504/1‑148 (MQ=255)
                                                                                     aGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCagaag                                                          <  2:524609/148‑1 (MQ=255)
                                                                                      gACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCagaaga                                                         >  2:3343973/1‑148 (MQ=255)
                                                                                      gACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCagaaga                                                         >  1:399330/1‑148 (MQ=255)
                                                                                      gACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCagaaga                                                         >  2:3422529/1‑148 (MQ=255)
                                                                                      gACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCagaaga                                                         >  1:552232/1‑148 (MQ=255)
                                                                                         cAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCt                                                      >  1:1738753/1‑148 (MQ=255)
                                                                                         cAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCt                                                      >  2:887322/1‑148 (MQ=255)
                                                                                          aaTACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCtt                                                     >  2:2389808/1‑148 (MQ=255)
                                                                                           aTACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCttt                                                    >  2:1252019/1‑148 (MQ=255)
                                                                                           aTACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAAAGACCAGAATATCtta                                                    >  2:1742649/1‑147 (MQ=255)
                                                                                           aTACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTGTCAACGACCCGAAGCTCttt                                                    >  1:1920452/1‑148 (MQ=255)
                                                                                                aaGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGc                                               >  1:2398542/1‑148 (MQ=255)
                                                                                                  gAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGAAGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGTCa                                             >  1:2586452/1‑148 (MQ=255)
                                                                                                   aaTGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAg                                            >  1:318172/1‑148 (MQ=255)
                                                                                                    aTGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGa                                           <  1:828022/148‑1 (MQ=255)
                                                                                                    aTGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGa                                           <  1:1102964/148‑1 (MQ=255)
                                                                                                      gggATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCATTAAGATTACCCCTATGTTGATACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGAcc                                         <  1:1545375/148‑1 (MQ=255)
                                                                                                           aaGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCg                                    >  1:2896715/1‑148 (MQ=255)
                                                                                                             ggATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGaa                                  >  2:1768989/1‑148 (MQ=255)
                                                                                                              gATGACACGGAACAGTGTAAGCGACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGaaa                                 >  1:264017/1‑148 (MQ=255)
                                                                                                              gATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCTAGACCTTGCGaaa                                 >  1:1854959/1‑148 (MQ=255)
                                                                                                              gATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGaaa                                 >  1:3206366/1‑148 (MQ=255)
                                                                                                              gATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGaaa                                 >  1:2394685/1‑148 (MQ=255)
                                                                                                                tGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATa                               <  1:2734265/148‑1 (MQ=255)
                                                                                                                 gACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATAc                              <  2:2839876/148‑1 (MQ=255)
                                                                                                                   cacGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATAccc                            >  1:2818698/1‑148 (MQ=255)
                                                                                                                   cacGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATAccc                            <  2:1869466/148‑1 (MQ=255)
                                                                                                                    acGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCg                           >  1:3267805/1‑148 (MQ=255)
                                                                                                                        aaCAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCAc                       >  1:1396147/1‑148 (MQ=255)
                                                                                                                         aCAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACt                      <  2:1880483/148‑1 (MQ=255)
                                                                                                                         aCAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACt                      <  2:1583084/148‑1 (MQ=255)
                                                                                                                           aGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACttt                    >  2:1017068/1‑148 (MQ=255)
                                                                                                                             tgtAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTg                  <  2:3500025/148‑1 (MQ=255)
                                                                                                                             tgtAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTg                  >  1:1315662/1‑148 (MQ=255)
                                                                                                                               tAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCa                >  1:3424976/1‑148 (MQ=255)
                                                                                                                               tAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCa                <  2:1517367/148‑1 (MQ=255)
                                                                                                                                aaGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCaa               >  2:1005157/1‑148 (MQ=255)
                                                                                                                                  gCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAAtt             >  2:1008398/1‑148 (MQ=255)
                                                                                                                                    aaCGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTc           <  2:975156/148‑1 (MQ=255)
                                                                                                                                    aaCGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTc           <  1:928393/148‑1 (MQ=255)
                                                                                                                                           ggATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGACg    >  2:2725678/1‑148 (MQ=255)
                                                                                                                                            gATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGACGc   <  2:3154720/148‑1 (MQ=255)
                                                                                                                                             atatAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGACGCt  >  1:1080200/1‑148 (MQ=255)
                                                                                                                                             atatAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGACGCt  >  1:1080180/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
AGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGACGCT  >  CP009273/1986144‑1986432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑