Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,002,354 C→A 100% L199L (CTC→CTA)  yedE → UPF0394 family sulfur transport domain‑containing inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,002,3540CA97.4% 363.4 / 1.4 148L199L (CTC→CTA) yedEUPF0394 family sulfur transport domain‑containing inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (2/3);  new base A (70/68);  total (77/71)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 150 total aligned reads displayed.

TTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTCGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGC  >  CP009273/2002209‑2002498
                                                                                                                                                 |                                                                                                                                                
ttGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTCTAgg                                                                                                                                                >  1:1940314/1‑148 (MQ=255)
     aTCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTCTAGGGCTgg                                                                                                                                           >  1:30757/1‑147 (MQ=255)
     aTCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTCTAGGGCTGc                                                                                                                                           >  1:30759/1‑148 (MQ=255)
      tCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCt                                                                                                                                          <  2:1404950/148‑1 (MQ=255)
         ccACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGt                                                                                                                                       <  1:2772917/148‑1 (MQ=255)
          cACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTc                                                                                                                                      <  2:2592529/148‑1 (MQ=255)
          cACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTc                                                                                                                                      <  2:2032225/148‑1 (MQ=255)
           aCTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTGGTGTAGGGATGGTGGTCt                                                                                                                                     >  1:732567/1‑148 (MQ=255)
           aCTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGtgt                                                                                                                                     >  1:1059652/1‑146 (MQ=255)
            cTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCtt                                                                                                                                    >  2:526979/1‑148 (MQ=255)
            cTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCtt                                                                                                                                    >  2:3222328/1‑148 (MQ=255)
              gCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTAtttt                                                                                                                                  >  1:585622/1‑148 (MQ=255)
                cATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTc                                                                                                                                <  2:1648784/148‑1 (MQ=255)
                 aTTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCg                                                                                                                               >  1:885439/1‑148 (MQ=255)
                 aTTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCg                                                                                                                               >  1:2665516/1‑148 (MQ=255)
                  ttGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTAGTCTAGGGATGCTGGTCTTTTTAgg                                                                                                                              >  2:3117368/1‑148 (MQ=255)
                      ttCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCAtt                                                                                                                          >  1:535767/1‑147 (MQ=255)
                      ttCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCCTCTAGGGGAGCTGGTGTTTTTCGGCaag                                                                                                                          >  1:257144/1‑146 (MQ=255)
                      ttCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTAGTCCCGTGAAAATGCAGAAAGTGTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATg                                                                                                                          <  2:2545028/148‑1 (MQ=255)
                      ttCATGGGTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATg                                                                                                                          <  2:2545019/148‑1 (MQ=255)
                       tCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGTCTAGGGATGCTGGTCTTTTTCGGCATgc                                                                                                                         >  1:2309136/1‑148 (MQ=255)
                               tGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTTGGGATGGTGGTGTTTTTCGGCATgctgggctgg                                                                                                                 >  1:3150119/1‑148 (MQ=255)
                                ggCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGctgggctggg                                                                                                                <  1:1078503/148‑1 (MQ=255)
                                 gcgcgcgcTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTGGGCTAGGGGTGCGGGTCTTTTTGGGCCTGCggggctgggc                                                                                                               >  1:499229/1‑148 (MQ=255)
                                  cgcgcgcTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGgcg                                                                                                              <  1:3190673/148‑1 (MQ=255)
                                    cgcgcTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCgct                                                                                                            >  2:3124670/1‑148 (MQ=255)
                                        cTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTGTAGGGATGCTGGTCTTTTTTGGCATTCTGGGGTGGGGGCTGCTc                                                                                                        >  1:1589226/1‑148 (MQ=255)
                                          ttACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGGCGAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTcac                                                                                                      >  2:1540491/1‑148 (MQ=255)
                                             cccTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTGGTGTTGGGGTGCTGGTGTTTTTTGGGATGGTGGGGGGGGGGGTGGTCACAGc                                                                                                   >  1:353179/1‑148 (MQ=255)
                                             cccTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGCTGCTGGTCTTTTTCGGCATGCGGGGCGGGGCGCTGCTCACAGc                                                                                                   >  2:268978/1‑148 (MQ=255)
                                             cccTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCGGGTGTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGc                                                                                                   >  1:3137417/1‑148 (MQ=255)
                                                    gcCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGaac                                                                                            <  1:705656/148‑1 (MQ=255)
                                                    gcCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGaac                                                                                            <  1:2962883/148‑1 (MQ=255)
                                                    gcCCATCTTCCGTAGTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGaac                                                                                            <  1:2487127/148‑1 (MQ=255)
                                                         tCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCGAGGGCTGCTGGTGGTTTTCGGCATGCTGGGCGGGGCGCTGCTCACAGCGATGaaccaacc                                                                                       >  2:1047034/1‑148 (MQ=255)
                                                          cTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAAccc                                                                                      >  2:2939576/1‑148 (MQ=255)
                                                           ttCCGTATTCCCGTGAAAATGCAGAATGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCa                                                                                     <  1:2942255/148‑1 (MQ=255)
                                                            tCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTAGTGTATGGGTGGTGGTCTTTTTGGGCATGGGGGGCTGGGCGGTGCTCACAGCGATGAaccaaaccaa                                                                                    >  1:91893/1‑148 (MQ=255)
                                                             ccGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTCGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCaaa                                                                                   <  1:1695604/148‑1 (MQ=255)
                                                             ccGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTCGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCaaa                                                                                   <  1:1695384/148‑1 (MQ=255)
                                                               gTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACt                                                                                 <  2:2019217/148‑1 (MQ=255)
                                                               gTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACt                                                                                 <  2:1093043/148‑1 (MQ=255)
                                                                tATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTGTAGGGGTTCGGGTGTTTTTAGGCATTCGGGGCTGGGCGCTGCTAACAGCGCTGAACCCACCCCAAcag                                                                                >  1:706786/1‑146 (MQ=255)
                                                                 aTTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTGGTCTAGGGGTGGGGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTgg                                                                               >  1:1168920/1‑148 (MQ=255)
                                                                 aTTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGAAGCTGGTCTTTTTCGGCATGCTGGGCAGGGCGCAGCTCACAGCCATGAACCAACCCAAACTgg                                                                               >  2:1741245/1‑148 (MQ=255)
                                                                 aTTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCGAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCCACCCAAACggg                                                                               >  2:1307939/1‑148 (MQ=255)
                                                                  ttCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTggg                                                                              >  2:2162021/1‑148 (MQ=255)
                                                                   tCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGGGGGGGGCGGCGGGTGTTTTTGGGCCTGCGGGGCGGGGCGCTGCGCACACCGCTGAACCACCCCCAACTgggg                                                                             >  1:1868921/1‑148 (MQ=255)
                                                                     ccGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTTGGGGAGGGGTGCGGGTGTTTTTTGGCCTGCAGGGGGGGGGGCTGGGGACACCGCAGAACCACACCCAACAGGGGcg                                                                           >  1:1566934/1‑147 (MQ=255)
                                                                      cGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGGTGCTGGTCTTTTTCGGCATGGTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTg                                                                          >  1:1777183/1‑148 (MQ=255)
                                                                      cGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTAGTGTAGGGATGCTGGTCTTTTTCGGCATGCTGGGGTGGGCGCGGCTCACAGCGATGAACCAACCCAAACAGGGGCTg                                                                          >  1:2180980/1‑148 (MQ=255)
                                                                      cGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGGGGTGGGGTGGTGGTCTTTTTCGGCCTGCTGGGCGGGGCGCTGCGCACAGCGCTGACCCAACCCAAACGGGGGCTg                                                                          >  1:391211/1‑148 (MQ=255)
                                                                       gTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTgg                                                                         <  1:230472/148‑1 (MQ=255)
                                                                         gAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCa                                                                       <  1:1611849/148‑1 (MQ=255)
                                                                         gAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCGCGGGGTGCTGGTCTTTTTGGGCATGCTGGGGGGGGCGCTGCTCACAGCGATGAACCAACCCACACAGGGGCTGGCa                                                                       >  1:3455243/1‑148 (MQ=255)
                                                                          aaaaTGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCaa                                                                      <  2:1740964/148‑1 (MQ=255)
                                                                          aaaaTGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCaa                                                                      <  2:1530145/148‑1 (MQ=255)
                                                                           aaaTGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAAt                                                                     <  1:2818565/148‑1 (MQ=255)
                                                                           aaaTGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAAt                                                                     <  2:2273320/148‑1 (MQ=255)
                                                                            aaTGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATg                                                                    <  1:1987484/148‑1 (MQ=255)
                                                                               gCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTg                                                                 <  2:22251/148‑1 (MQ=255)
                                                                                   aaaGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTg                                                             <  2:74431/148‑1 (MQ=255)
                                                                                      gTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTGTTTTTCGGCATGCTGGGGTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCg                                                          >  1:2074694/1‑148 (MQ=255)
                                                                                       tttCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGt                                                         <  2:2470223/148‑1 (MQ=255)
                                                                                           tGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGc                                                     <  2:885439/148‑1 (MQ=255)
                                                                                            gccgccTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTGTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCCAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCt                                                    >  2:2783293/1‑148 (MQ=255)
                                                                                            gccgccTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGTCGAGGGCTGCGGGTGTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCGGTTTTGCGGCGGCt                                                    >  1:746257/1‑148 (MQ=255)
                                                                                             ccgccTCACCGCTGACGCAAAAACCGGATCCGGCGCGGCGTCGTGTTCGTCTCGGGGTGCGGGTGTTTTTCGGCCTGCGGGGCGGGGCGCGGCGCACAGCGATGAACCAACCCAAACGGGGGCTGGCCATGGTGTGTGGCGTCGGcgg                                                   >  2:1589785/1‑146 (MQ=255)
                                                                                             ccgccTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGCTGGTGGTCTTTTTCGGCATGGTGGGGTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCtt                                                   >  2:2731291/1‑148 (MQ=255)
                                                                                                ccTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTgg                                                <  2:1868921/148‑1 (MQ=255)
                                                                                                 cTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTGGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGGTGGTTGGCGTCGGGGTTGGt                                               >  1:262114/1‑148 (MQ=255)
                                                                                                     ccGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTGGTCTAGGGATGCTGGTGTTTTTGGGCATGCTGGGCTGGGCGCTGCTCACAGCGCTGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTAc                                           >  2:2096512/1‑148 (MQ=255)
                                                                                                     ccGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTGTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTAc                                           <  1:1154962/148‑1 (MQ=255)
                                                                                                      cGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTGTAGGGATGCTGGTCTTTTTCGGCATGGTGGGGGGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGGGTCGGCTTTGGTTTaat                                          >  1:2185082/1‑146 (MQ=255)
                                                                                                       gCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTg                                         <  1:2952419/148‑1 (MQ=255)
                                                                                                       gCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTg                                         <  1:2885353/148‑1 (MQ=255)
                                                                                                         tGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGAt                                       <  2:3254781/148‑1 (MQ=255)
                                                                                                           aCGCAAAAACCGGATCAGGCGCGGCGTCGTTTTGGGCTAGGGATGCGGGTCTTTTTCGGCATGCGGGGCGGGGCGCTGCTCACACCGATGAACCAACCCAAACAGGGGCGGGCAATGCTGTTTGGGGGCGGCTTTGGGTTTCTGATTg                                     >  1:2856752/1‑148 (MQ=255)
                                                                                                           aCGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTGTTGGGGTGCTGGTCTTTTTAGGCCTGCTGGGGGGGGCGCTGCGCACAGCGCTGGAACAACCCAAACTGGGGGTGGCAATGCTGTTTGGCGTGGGGTTTGGTTTAATGAttt                                     >  1:1148057/1‑147 (MQ=255)
                                                                                                            cGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGa                                    <  1:3140414/148‑1 (MQ=255)
                                                                                                               aaaaaCCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACg                                 <  2:961676/148‑1 (MQ=255)
                                                                                                                aaaaCCGGATCAGGAGAGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGTGGCTGGGAATGCTTTTTGGCGTCGGATTTGGTTTACTGATTGAACGt                                <  1:1741245/148‑1 (MQ=255)
                                                                                                                  aaCCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCCTGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGGTTGGCGTCGGCTTTGGTTTACTGATTGAACGTgc                              >  2:572853/1‑148 (MQ=255)
                                                                                                                  aaCCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTgc                              >  2:3538223/1‑148 (MQ=255)
                                                                                                                  aaCCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCGGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTgc                              >  2:3538147/1‑148 (MQ=255)
                                                                                                                         tCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGGTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTAATGATTGAACGTGCGCAAATc                       >  2:3450557/1‑148 (MQ=255)
                                                                                                                           aGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTg                     <  2:3046597/148‑1 (MQ=255)
                                                                                                                            ggCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGc                    <  1:1840401/148‑1 (MQ=255)
                                                                                                                             gcgcGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGAGGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCt                   <  2:2391489/148‑1 (MQ=255)
                                                                                                                             gcgcGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTAGGCATGCTGGGCTTGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCGGGCAATTCTGTTTTGCGTCGGCTTTGGTTTAATGATTGAACGTGCGCAAATCTGCt                   >  2:40610/1‑148 (MQ=255)
                                                                                                                               gcggcgTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCttt                 <  2:3347548/148‑1 (MQ=255)
                                                                                                                                cggcgTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTa                <  2:2587819/148‑1 (MQ=255)
                                                                                                                                 ggcgTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTAc               <  1:2630573/148‑1 (MQ=255)
                                                                                                                                  gcgTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACt              <  2:2922934/148‑1 (MQ=255)
                                                                                                                                       gtTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGc         <  1:2516690/148‑1 (MQ=255)
                                                                                                                                       gtTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGc         <  1:2514089/148‑1 (MQ=255)
                                                                                                                                         tttCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGt       >  1:2106815/1‑148 (MQ=255)
                                                                                                                                            cGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTcc    >  2:1911679/1‑148 (MQ=255)
                                                                                                                                              tctaGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCcgc  >  2:758872/1‑148 (MQ=255)
                                                                                                                                              tctaGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCcgc  >  2:762649/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTCGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGC  >  CP009273/2002209‑2002498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑