BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	186,159	A→G	100%	intergenic (‑166/‑40)	map ← / → tff	methionine aminopeptidase/novel sRNA, function unknown

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	186,159	0	A	G	98.4%	957.7 / 1.1	242	intergenic (‑166/‑40)	map/tff	methionine aminopeptidase/novel sRNA, function unknown
Reads supporting (aligned to +/- strand): ref base A (3/1); new base G (147/91); total (150/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 249 total aligned reads displayed.

TGATACCGACAATATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAATTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGG  >  CP009273/186016‑186302

tGATACCGACAATATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAgttat                                                                                                                                             >  2:487515/1‑148 (MQ=255)

tGATACCGACAATATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAgttat                                                                                                                                             >  1:1519681/1‑148 (MQ=255)

  aTACCGACAATATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTg                                                                                                                                           <  2:3443982/148‑1 (MQ=255)

  aTACCGACAATATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTg                                                                                                                                           >  2:2553214/1‑148 (MQ=255)

  aTACCGACAATATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAATTATTg                                                                                                                                           <  2:809464/148‑1 (MQ=255)

    aCCGACAATATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGgt                                                                                                                                         >  2:1705120/1‑148 (MQ=255)

    aCCGACAATATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGgt                                                                                                                                         >  1:3154831/1‑148 (MQ=255)

     ccGACAATATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGgtg                                                                                                                                        <  2:551231/148‑1 (MQ=255)

     ccGACAATATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGgtg                                                                                                                                        >  2:3493922/1‑148 (MQ=255)

       gACAATATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGTTGTACACAGCGCCAACAGTTATTGGTGTc                                                                                                                                      <  1:396331/148‑1 (MQ=255)

       gACAATATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTc                                                                                                                                      <  2:187153/148‑1 (MQ=255)

        aCAATATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTcc                                                                                                                                     >  2:101547/1‑148 (MQ=255)

               atatGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGGTATTGGTGTCCACGACGt                                                                                                                              >  2:3486892/1‑148 (MQ=255)

                 atGTAAGTGCCGTCAATGGTATCACACCCGGGCACATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTAt                                                                                                                            <  2:1801466/148‑1 (MQ=255)

                 atGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTAt                                                                                                                            >  2:988796/1‑148 (MQ=255)

                   gTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATACTAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTAttt                                                                                                                          <  1:2362665/148‑1 (MQ=255)

                     aaGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTtgt                                                                                                                        >  2:925302/1‑148 (MQ=255)

                      aGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTtgtg                                                                                                                       >  1:1338873/1‑148 (MQ=255)

                      aGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTtgtg                                                                                                                       <  2:2567353/148‑1 (MQ=255)

                          ccGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGtat                                                                                                                   >  1:3473702/1‑148 (MQ=255)

                            gTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATaa                                                                                                                 <  2:630860/148‑1 (MQ=255)

                             tCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATaaa                                                                                                                <  2:3227975/148‑1 (MQ=255)

                              cAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAg                                                                                                               >  2:1669426/1‑148 (MQ=255)

                                aTGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAgcg                                                                                                             <  1:2133328/148‑1 (MQ=255)

                                 tGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAgcgc                                                                                                            >  1:2109723/1‑148 (MQ=255)

                                 tGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGGTATTGGTGTCCACGACGTATTTGTGGTATAAAgcgc                                                                                                            >  2:85339/1‑148 (MQ=255)

                                  ggTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAgcgcg                                                                                                           <  1:2658291/148‑1 (MQ=255)

                                   gTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAgcgcgc                                                                                                          <  2:1587833/148‑1 (MQ=255)

                                    tATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGcc                                                                                                         >  1:826596/1‑148 (MQ=255)

                                         cacCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGGTATTGGTGTCCACGACGTATTTGGGGTATAAAGCGCGCCGGACt                                                                                                    >  2:2284986/1‑148 (MQ=255)

                                          acCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACtt                                                                                                   <  1:2394530/148‑1 (MQ=255)

                                          acCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACtt                                                                                                   <  1:2428510/148‑1 (MQ=255)

                                           cccGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTc                                                                                                  <  2:903442/148‑1 (MQ=255)

                                            ccGGTCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCCACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTcc                                                                                                 <  1:1836231/148‑1 (MQ=255)

                                            ccGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTcc                                                                                                 <  2:2600025/148‑1 (MQ=255)

                                               ggCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGAt                                                                                              >  1:22232/1‑148 (MQ=255)

                                                gCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATc                                                                                             >  2:2677180/1‑148 (MQ=255)

                                                gCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATc                                                                                             <  1:1459999/148‑1 (MQ=255)

                                                   aaTTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACCGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCAt                                                                                          <  2:352074/148‑1 (MQ=255)

                                                    aTTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCAtt                                                                                         >  2:2787307/1‑148 (MQ=255)

                                                     ttGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCAttt                                                                                        >  2:1767724/1‑148 (MQ=255)

                                                         gaATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGta                                                                                    >  1:1740759/1‑148 (MQ=255)

                                                          aaTCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGtat                                                                                   <  2:2208755/148‑1 (MQ=255)

                                                          aaTCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGtat                                                                                   <  1:1157439/148‑1 (MQ=255)

                                                          aaTCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGtat                                                                                   <  1:1159347/148‑1 (MQ=255)

                                                            tCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATac                                                                                 >  2:2215204/1‑148 (MQ=255)

                                                            tCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATac                                                                                 >  2:3353666/1‑148 (MQ=255)

                                                            tCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATac                                                                                 >  1:3360877/1‑148 (MQ=255)

                                                                 cTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGa                                                                            >  2:2126457/1‑148 (MQ=255)

                                                                 cTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGa                                                                            <  2:1881103/148‑1 (MQ=255)

                                                                    aaTTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTg                                                                         <  2:3018914/148‑1 (MQ=255)

                                                                     aTTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTgg                                                                        >  2:118995/1‑148 (MQ=255)

                                                                     aTTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTgg                                                                        >  2:3079662/1‑148 (MQ=255)

                                                                     aTTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTgg                                                                        >  2:779703/1‑148 (MQ=255)

                                                                      tttCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGa                                                                       <  2:337185/148‑1 (MQ=255)

                                                                      tttCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGa                                                                       >  2:1983613/1‑148 (MQ=255)

                                                                      tttCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGa                                                                       >  2:1582598/1‑148 (MQ=255)

                                                                       ttCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGAc                                                                      >  2:2506252/1‑148 (MQ=255)

                                                                           ccAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGaa                                                                  >  1:3235648/1‑148 (MQ=255)

                                                                           ccAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGaa                                                                  >  1:2591468/1‑148 (MQ=255)

                                                                              aaCGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCg                                                               >  1:643992/1‑148 (MQ=255)

                                                                               aCGTGCCACTGAAGGTTTTCTATAATATAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGa                                                              >  1:2807149/1‑148 (MQ=255)

                                                                               aCGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGa                                                              >  2:560664/1‑148 (MQ=255)

                                                                               aCGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGa                                                              >  2:1307503/1‑148 (MQ=255)

                                                                               aCGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAATTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGa                                                              >  1:2306525/1‑148 (MQ=255)

                                                                                cGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGAc                                                             <  2:2700924/148‑1 (MQ=255)

                                                                                 gTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACATACTGGACGGAAGCGACa                                                            <  2:3154831/148‑1 (MQ=255)

                                                                                        tGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCAc                                                     >  1:289898/1‑148 (MQ=255)

                                                                                            tgTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTtg                                                 >  2:1091609/2‑148 (MQ=255)

                                                                                             gTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTtgt                                                <  2:1876611/148‑1 (MQ=255)

                                                                                              ttttCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTtgtg                                               >  2:2169526/1‑148 (MQ=255)

                                                                                              ttttCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTtgtg                                               <  1:1983613/148‑1 (MQ=255)

                                                                                               tttCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTgtgt                                              <  2:1465822/148‑1 (MQ=255)

                                                                                                ttCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTa                                             >  2:118784/1‑148 (MQ=255)

                                                                                                     taataGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTaacaac                                        <  1:78233/148‑1 (MQ=255)

                                                                                                      aataGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAacaaca                                       >  2:2921371/1‑148 (MQ=255)

                                                                                                      aataGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAacaaca                                       >  1:479964/1‑148 (MQ=255)

                                                                                                      aataGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAacaaca                                       >  2:2456188/1‑148 (MQ=255)

                                                                                                        taGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGTCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAacaca                                     <  1:3448678/148‑1 (MQ=255)

                                                                                                        taGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAacaca                                     >  2:2738952/1‑148 (MQ=255)

                                                                                                        taGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAacaca                                     >  1:1272716/1‑148 (MQ=255)

                                                                                                           aaaaTTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAacacacac                                  >  2:1491991/1‑148 (MQ=255)

                                                                                                           aaaaTTCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAacacacac                                  >  2:3208032/1‑148 (MQ=255)

                                                                                                               ttCGACGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTAt                              <  1:497961/148‑1 (MQ=255)

                                                                                                                   aCGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGc                          >  2:895757/1‑148 (MQ=255)

                                                                                                                    cGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGca                         >  1:1362966/1‑148 (MQ=255)

                                                                                                                      tCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGcaca                       <  1:559228/148‑1 (MQ=255)

                                                                                                                       cTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACat                      >  2:3335442/1‑148 (MQ=255)

                                                                                                                          aTGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATAtt                   <  1:376868/148‑1 (MQ=255)

                                                                                                                           tGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTc                  >  2:1933973/1‑148 (MQ=255)

                                                                                                                           tGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTc                  >  1:1986409/1‑148 (MQ=255)

                                                                                                                             cTGTACACAGCGCCAACAGTTATTGGTTTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCg                <  1:1017457/148‑1 (MQ=255)

                                                                                                                             cTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCg                <  2:3736/148‑1 (MQ=255)

                                                                                                                             cTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCg                >  1:2703703/1‑148 (MQ=255)

                                                                                                                              tGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCgg               >  2:1733794/1‑148 (MQ=255)

                                                                                                                                tACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCgggg             >  2:747629/1‑148 (MQ=255)

                                                                                                                                  cacaGCGCCAACAGTTATTGGTGTCCACGACGTATTGGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTg           <  2:2097395/148‑1 (MQ=255)

                                                                                                                                       cgcCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCtt      <  1:1661456/148‑1 (MQ=255)

                                                                                                                                       cgcCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCtt      >  1:2431132/1‑148 (MQ=255)

                                                                                                                                           aacagTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTggg  >  2:1248215/1‑148 (MQ=255)

TGATACCGACAATATATATGTAAGTGCCGTCAATGGTATCACACCCGGGCAAATTGAGAATCATTCTGAATTTCGCCAAACGTGCCACTGAAGGTTTTCTATAATAGAAAATTCGACGTCTGATGCTGTACACAGCGCCAACAATTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGG  >  CP009273/186016‑186302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`