Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,041,346 C→A 94.9% I976I (ATC→ATA)  yeeJ → putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,041,3460CA94.9% 573.2 / 20.7 198I976I (ATC→ATA) yeeJputative adhesin
Reads supporting (aligned to +/- strand):  ref base C (3/7);  new base A (99/89);  total (102/96)
Fisher's exact test for biased strand distribution p-value = 2.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01

Only 100 of 198 total aligned reads displayed.

AGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTGGCTAAC  >  CP009273/2041201‑2041490
                                                                                                                                                 |                                                                                                                                                
  gTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCt                                                                                                                                              <  2:1092860/148‑1 (MQ=255)
   tGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCtt                                                                                                                                             <  1:1469622/148‑1 (MQ=255)
   tGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCtt                                                                                                                                             <  1:2763364/148‑1 (MQ=255)
     aaTTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGAAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTtct                                                                                                                                           <  2:2227128/148‑1 (MQ=255)
        tttATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCtg                                                                                                                                        >  1:3373291/1‑148 (MQ=255)
        tttATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCtg                                                                                                                                        >  1:191388/1‑148 (MQ=255)
            tCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGcc                                                                                                                                    >  2:3227597/1‑148 (MQ=255)
               gTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCaaa                                                                                                                                 <  2:24342/148‑1 (MQ=255)
                 gATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAacg                                                                                                                               <  1:3208400/148‑1 (MQ=255)
                  aTCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAacga                                                                                                                              <  2:1636994/148‑1 (MQ=255)
                   tCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAacgac                                                                                                                             >  2:1909947/1‑148 (MQ=255)
                     aaaGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGt                                                                                                                           >  1:2427076/1‑148 (MQ=255)
                      aaGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTc                                                                                                                          >  2:182982/1‑148 (MQ=255)
                         tACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCa                                                                                                                       >  1:167949/1‑148 (MQ=255)
                         tACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTACAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCa                                                                                                                       >  2:1057763/1‑148 (MQ=255)
                          aCTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCaa                                                                                                                      <  2:3043762/148‑1 (MQ=255)
                            tgctgcCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGTATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCaagt                                                                                                                    <  1:2162888/148‑1 (MQ=255)
                             gctgcCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCaagta                                                                                                                   <  2:1362274/148‑1 (MQ=255)
                                gcCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAATAAATAACCTTCTCTGTGCCAAACGACGTCGCaagtaagt                                                                                                                <  1:2470094/148‑1 (MQ=255)
                                 cccTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGtt                                                                                                               <  2:901342/148‑1 (MQ=255)
                                       cccTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGAt                                                                                                         <  2:1975236/148‑1 (MQ=255)
                                       cccTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGAt                                                                                                         >  1:1489002/1‑148 (MQ=255)
                                        ccTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGAtt                                                                                                        <  2:2052535/148‑1 (MQ=255)
                                            aGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTTCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCa                                                                                                    <  2:1754109/148‑1 (MQ=255)
                                            aGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCa                                                                                                    >  2:1135457/1‑148 (MQ=255)
                                            aGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCa                                                                                                    <  1:593281/148‑1 (MQ=255)
                                             gtgtGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCaa                                                                                                   >  1:2405723/1‑148 (MQ=255)
                                             gtgtGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCaa                                                                                                   >  2:732146/1‑148 (MQ=255)
                                             gtgtGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCaa                                                                                                   <  1:1176305/148‑1 (MQ=255)
                                             gtgtGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCaa                                                                                                   >  2:1600963/1‑148 (MQ=255)
                                              tgtgCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAAc                                                                                                  >  1:2669137/1‑148 (MQ=255)
                                                      cAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGaaa                                                                                          <  2:310969/148‑1 (MQ=255)
                                                         gTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAACGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAgg                                                                                       <  1:709151/148‑1 (MQ=255)
                                                         gTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAgg                                                                                       <  2:789670/148‑1 (MQ=255)
                                                          tGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGc                                                                                      >  1:642031/1‑148 (MQ=255)
                                                             tatCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATg                                                                                   <  1:1939354/148‑1 (MQ=255)
                                                             tatCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATg                                                                                   <  1:1005959/148‑1 (MQ=255)
                                                             tatCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATg                                                                                   <  1:3427360/148‑1 (MQ=255)
                                                             tatCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATg                                                                                   <  1:2549580/148‑1 (MQ=255)
                                                                cACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACg                                                                                >  2:393033/1‑148 (MQ=255)
                                                                cACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACg                                                                                >  1:3464819/1‑148 (MQ=255)
                                                                  ccGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGa                                                                              >  1:2809128/1‑148 (MQ=255)
                                                                   cGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCACGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGAt                                                                             >  1:2051931/1‑148 (MQ=255)
                                                                     tCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGTATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAg                                                                           <  2:806870/148‑1 (MQ=255)
                                                                      cACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGt                                                                          >  1:1897928/1‑148 (MQ=255)
                                                                       aCCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTa                                                                         <  2:1651686/148‑1 (MQ=255)
                                                                       aCCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTa                                                                         <  2:2615849/148‑1 (MQ=255)
                                                                         cAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAAc                                                                       >  1:962259/1‑148 (MQ=255)
                                                                          aaCACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACg                                                                      >  2:1033211/1‑148 (MQ=255)
                                                                           acacAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAACGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACgg                                                                     <  1:488988/148‑1 (MQ=255)
                                                                           acacAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACgg                                                                     >  1:936826/1‑148 (MQ=255)
                                                                           acacAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGACAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACgg                                                                     >  1:3061317/1‑148 (MQ=255)
                                                                            cacaGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACggg                                                                    >  1:569186/1‑148 (MQ=255)
                                                                                gCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTg                                                                >  2:1056319/1‑148 (MQ=255)
                                                                                 cTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGc                                                               >  1:100935/1‑148 (MQ=255)
                                                                                  tCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCa                                                              <  2:2860861/148‑1 (MQ=255)
                                                                                   ccGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCaa                                                             >  2:3163381/1‑148 (MQ=255)
                                                                                    cGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAAt                                                            >  2:974352/1‑148 (MQ=255)
                                                                                    cGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAAt                                                            >  1:3312018/1‑148 (MQ=255)
                                                                                    cGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAAt                                                            <  2:2996001/148‑1 (MQ=255)
                                                                                      cAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCg                                                          >  1:3432545/1‑148 (MQ=255)
                                                                                          atatGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTc                                                      <  1:3513689/148‑1 (MQ=255)
                                                                                             tGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCt                                                   >  2:814670/1‑148 (MQ=255)
                                                                                                    aaCCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGc                                            <  2:191388/148‑1 (MQ=255)
                                                                                                     aCCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCa                                           >  1:1348617/1‑148 (MQ=255)
                                                                                                         tGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGtt                                       <  1:1909947/148‑1 (MQ=255)
                                                                                                          gCAGTATAAAAATGGCAACCCACTAAAACATAAACAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTa                                      <  1:321016/148‑1 (MQ=255)
                                                                                                          gCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCACTCGCCTCCCTGACCGGCACGTTa                                      >  2:1994742/1‑148 (MQ=255)
                                                                                                          gCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTa                                      <  1:3140449/148‑1 (MQ=255)
                                                                                                           cAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAg                                     >  1:536998/1‑148 (MQ=255)
                                                                                                           cAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAg                                     >  1:1218464/1‑148 (MQ=255)
                                                                                                              gATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCgg                                  <  2:3431870/148‑1 (MQ=255)
                                                                                                              gATAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCAAAACGACGTCGCAAGTAAGTTCTCGAGTAGCAACGGAGGAAAAGGCATGACGGATCGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCgg                                  >  2:2119254/1‑148 (MQ=255)
                                                                                                               aTAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCCAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCggg                                 <  1:182982/148‑1 (MQ=255)
                                                                                                               aTAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCggg                                 <  1:1980230/148‑1 (MQ=255)
                                                                                                                tAAAAATGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGc                                <  2:1423717/148‑1 (MQ=255)
                                                                                                                    aaTGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGc                            <  2:1741998/148‑1 (MQ=255)
                                                                                                                     aTGGCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCa                           >  2:2327558/1‑148 (MQ=255)
                                                                                                                        gCAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCatat                        <  2:3464819/148‑1 (MQ=255)
                                                                                                                         cAACCCACTAAAAGATAAAGAAATACCCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATg                       <  1:149041/148‑1 (MQ=255)
                                                                                                                         cAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATg                       <  1:125473/148‑1 (MQ=255)
                                                                                                                         cAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATg                       <  2:2060954/148‑1 (MQ=255)
                                                                                                                         cAACCCACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATg                       <  1:1946747/148‑1 (MQ=255)
                                                                                                                            cccACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATc                    >  1:2016744/1‑148 (MQ=255)
                                                                                                                              cACTAAAAGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCAt                  <  1:3227597/148‑1 (MQ=255)
                                                                                                                                    aaGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCg            >  1:1800143/1‑148 (MQ=255)
                                                                                                                                     aGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGt           <  2:1019264/148‑1 (MQ=255)
                                                                                                                                     aGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGt           <  2:1500041/148‑1 (MQ=255)
                                                                                                                                     aGATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGt           >  2:2081461/1‑148 (MQ=255)
                                                                                                                                      gATAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTc          >  1:447824/1‑148 (MQ=255)
                                                                                                                                       aTAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCt         >  2:491316/1‑148 (MQ=255)
                                                                                                                                        tAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTg        <  1:3163381/148‑1 (MQ=255)
                                                                                                                                        tAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTg        >  2:1394817/1‑148 (MQ=255)
                                                                                                                                        tAAAGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTg        >  1:3152116/1‑148 (MQ=255)
                                                                                                                                         aaaGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTgg       >  2:2459742/1‑148 (MQ=255)
                                                                                                                                         aaaGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTgg       >  1:3115718/1‑148 (MQ=255)
                                                                                                                                           aGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTGGCt     <  2:642031/148‑1 (MQ=255)
                                                                                                                                           aGAAATAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTGGCt     >  2:1816431/1‑148 (MQ=255)
                                                                                                                                             aaaTAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTGGCTaa   <  2:3123445/148‑1 (MQ=255)
                                                                                                                                             aaaTAACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTGGCTaa   <  1:2982006/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AGGTGAATTTTATCGGTGATCAAAGTACTGCTGCCCTGACCCTCAGTGTGCCTTCAGGTGATATCACCGTCACCAACACAGCTCCGCAATATATGACTGCAACCTTGCAGGATAAAAATGGCAACCCACTAAAAGATAAAGAAATCACCTTCTCTGTGCCAAACGACGTCGCAAGTAAGTTCTCGATTAGCAACGGAGGAAAAGGCATGACGGATAGTAACGGGGTTGCAATCGCCTCCCTGACCGGCACGTTAGCGGGCACGCATATGATCATGGCTCGTCTGGCTAAC  >  CP009273/2041201‑2041490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑