Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,101,912 T→G 100% E385A (GAG→GCG)  wzxB ← putative polisoprenol‑linked O‑antigen transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,101,9120TG96.6% 915.8 / 7.2 236E385A (GAG→GCG) wzxBputative polisoprenol‑linked O‑antigen transporter
Reads supporting (aligned to +/- strand):  ref base T (4/4);  new base G (104/124);  total (108/128)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 242 total aligned reads displayed.

ACGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCA  >  CP009273/2101767‑2102056
                                                                                                                                                 |                                                                                                                                                
aCGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATcgct                                                                                                                                                <  1:2777365/148‑1 (MQ=255)
aCGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATcgct                                                                                                                                                <  1:3300968/148‑1 (MQ=255)
aCGGCACCAAACAAACCAGAACCAACAATGATATAATCGTAAATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATcgct                                                                                                                                                <  1:3266770/148‑1 (MQ=255)
 cGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCtt                                                                                                                                               >  2:24970/1‑148 (MQ=255)
   gCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTa                                                                                                                                             >  1:237417/1‑148 (MQ=255)
      ccAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTaaaa                                                                                                                                          <  2:2917325/148‑1 (MQ=255)
       caaacaaaCCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTaaaaa                                                                                                                                         >  1:1053539/1‑148 (MQ=255)
        aaacaaacCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTaaaaat                                                                                                                                        >  2:1680642/1‑147 (MQ=255)
        aaacaaacCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAag                                                                                                                                        >  1:2240371/1‑148 (MQ=255)
          acaaacCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCACTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAagag                                                                                                                                      <  2:2725930/148‑1 (MQ=255)
             aacCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAgttg                                                                                                                                   <  1:595397/148‑1 (MQ=255)
             aacCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAgttg                                                                                                                                   >  2:2868589/1‑148 (MQ=255)
               ccAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAgttgtt                                                                                                                                 >  1:183316/1‑148 (MQ=255)
               ccAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAgttgtt                                                                                                                                 >  1:1980752/1‑148 (MQ=255)
                cAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTa                                                                                                                                <  2:18926/148‑1 (MQ=255)
                 aGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAg                                                                                                                               >  2:608449/1‑148 (MQ=255)
                 aGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAg                                                                                                                               <  2:3203019/148‑1 (MQ=255)
                   aaCCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCg                                                                                                                             >  2:268614/1‑148 (MQ=255)
                   aaCCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCg                                                                                                                             >  2:2157499/1‑148 (MQ=255)
                    aCCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCgg                                                                                                                            <  2:312133/148‑1 (MQ=255)
                      caacaaTGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGaa                                                                                                                          >  1:182865/1‑148 (MQ=255)
                       aacaaTGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGaaa                                                                                                                         >  2:393425/1‑148 (MQ=255)
                           aTGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATc                                                                                                                     >  2:2980973/1‑148 (MQ=255)
                           aTGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATc                                                                                                                     >  2:2395690/1‑148 (MQ=255)
                               tataATCGTACATAAAAGCCTCCGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATcaaca                                                                                                                 <  2:2247738/148‑1 (MQ=255)
                                   aTCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATcaacaacaa                                                                                                             >  2:1655232/1‑148 (MQ=255)
                                     cGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAAc                                                                                                           <  1:2283388/148‑1 (MQ=255)
                                        aCATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTca                                                                                                        >  1:675924/1‑148 (MQ=255)
                                         cATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTcaa                                                                                                       <  2:51499/148‑1 (MQ=255)
                                           tAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTcaaca                                                                                                     >  1:2889942/1‑148 (MQ=255)
                                           tAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTcaaca                                                                                                     >  1:1115241/1‑148 (MQ=255)
                                              aaTCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAAc                                                                                                  <  2:1433920/148‑1 (MQ=255)
                                              aaTCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAAc                                                                                                  >  1:2949682/1‑148 (MQ=255)
                                              aaTCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAAc                                                                                                  >  2:774226/1‑148 (MQ=255)
                                              aaTCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAAc                                                                                                  >  2:3407607/1‑148 (MQ=255)
                                               aTCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAAcc                                                                                                 >  2:1443083/1‑148 (MQ=255)
                                               aTCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAAcc                                                                                                 <  2:840820/148‑1 (MQ=255)
                                               aTCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAAcc                                                                                                 <  1:2343103/148‑1 (MQ=255)
                                                 ccTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCg                                                                                               >  1:1741585/1‑148 (MQ=255)
                                                  cTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCTATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGc                                                                                              <  2:1822319/148‑1 (MQ=255)
                                                    cAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAg                                                                                            <  2:960751/148‑1 (MQ=255)
                                                      gCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCg                                                                                          >  1:21039/1‑148 (MQ=255)
                                                       cAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGa                                                                                         >  1:2907117/1‑148 (MQ=255)
                                                         aaCCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGAtt                                                                                       <  1:3202748/148‑1 (MQ=255)
                                                         aaCCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGAtt                                                                                       <  2:2468806/148‑1 (MQ=255)
                                                            cAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTaaa                                                                                    <  1:1168757/148‑1 (MQ=255)
                                                              gTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAAt                                                                                  >  2:118256/1‑148 (MQ=255)
                                                                aaTTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCt                                                                                >  2:3105392/1‑148 (MQ=255)
                                                                  tttATTAATTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTa                                                                              >  1:2825441/1‑148 (MQ=255)
                                                                   ttattaTTTCGTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTAc                                                                             <  1:2400081/148‑1 (MQ=255)
                                                                    tattatTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACt                                                                            >  1:2067576/1‑148 (MQ=255)
                                                                    tattatTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAAACCGCAGCGATTAAAATCTTACt                                                                            >  2:2791794/1‑148 (MQ=255)
                                                                     attattTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTg                                                                           <  1:1321498/148‑1 (MQ=255)
                                                                      ttattTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGa                                                                          <  2:2339641/148‑1 (MQ=255)
                                                                      ttattTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGa                                                                          <  1:2415188/148‑1 (MQ=255)
                                                                       tattTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGaa                                                                         >  2:888650/1‑148 (MQ=255)
                                                                       tattTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGaa                                                                         <  1:2554047/148‑1 (MQ=255)
                                                                         tttCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAAtt                                                                       <  2:1115241/148‑1 (MQ=255)
                                                                               aCGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAAttctttct                                                                 <  1:1210038/148‑1 (MQ=255)
                                                                                cGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATtctttctt                                                                <  1:1564553/148‑1 (MQ=255)
                                                                                cGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATtctttctt                                                                <  1:3325737/148‑1 (MQ=255)
                                                                                cGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATtctttctt                                                                >  1:986601/1‑148 (MQ=255)
                                                                                cGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATtctttctt                                                                >  2:1012316/1‑148 (MQ=255)
                                                                                   aCATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTtata                                                             <  2:2240183/148‑1 (MQ=255)
                                                                                    cATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATaa                                                            <  2:3290044/148‑1 (MQ=255)
                                                                                      tCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAAtt                                                          >  1:3459074/1‑148 (MQ=255)
                                                                                       cAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTa                                                         <  1:1540523/148‑1 (MQ=255)
                                                                                        aGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTAt                                                        >  2:2657849/1‑148 (MQ=255)
                                                                                        aGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTAt                                                        <  2:255438/148‑1 (MQ=255)
                                                                                        aGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTAt                                                        <  2:2049677/148‑1 (MQ=255)
                                                                                         gCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATg                                                       <  1:2066574/148‑1 (MQ=255)
                                                                                         gCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATg                                                       <  2:3390472/148‑1 (MQ=255)
                                                                                          cATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATgg                                                      >  2:2607719/1‑148 (MQ=255)
                                                                                           aTGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATggg                                                     <  1:452983/148‑1 (MQ=255)
                                                                                            tGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGt                                                    <  2:1537458/148‑1 (MQ=255)
                                                                                            tGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGt                                                    <  2:1848977/148‑1 (MQ=255)
                                                                                            tGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACAAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGt                                                    <  1:534702/148‑1 (MQ=255)
                                                                                             gAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTc                                                   >  2:3463410/1‑148 (MQ=255)
                                                                                                 gACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCa                                               <  1:2995566/148‑1 (MQ=255)
                                                                                                      aaCTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACt                                          >  1:1407296/1‑148 (MQ=255)
                                                                                                         tAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGa                                       <  1:791982/148‑1 (MQ=255)
                                                                                                           aGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAAt                                     >  2:665332/1‑148 (MQ=255)
                                                                                                            gCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATg                                    <  2:23578/148‑1 (MQ=255)
                                                                                                              aCTCTGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGcc                                  >  1:615789/1‑148 (MQ=255)
                                                                                                               ctctGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCa                                 >  2:3238018/1‑148 (MQ=255)
                                                                                                                  tGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAg                              <  1:2003505/148‑1 (MQ=255)
                                                                                                                  tGTTGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAg                              <  2:183316/148‑1 (MQ=255)
                                                                                                                    ttGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGAc                            >  1:3399858/1‑148 (MQ=255)
                                                                                                                     tGCAAGCAATGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACa                           <  1:1279668/148‑1 (MQ=255)
                                                                                                                         agcaaTGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTAc                       >  1:2514582/1‑148 (MQ=255)
                                                                                                                          gcaaTGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACt                      >  1:3120884/1‑148 (MQ=255)
                                                                                                                            aaTGTTATTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTa                    >  2:1503355/1‑148 (MQ=255)
                                                                                                                                  aTTGCTGCACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGaaa              <  1:1729051/148‑1 (MQ=255)
                                                                                                                                      ctgcACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAg          <  1:3414372/148‑1 (MQ=255)
                                                                                                                                       tgcACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGa         <  2:1248492/148‑1 (MQ=255)
                                                                                                                                         cACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACACACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGaaa       <  2:1364539/148‑1 (MQ=255)
                                                                                                                                         cACCAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGaaa       >  1:217849/1‑148 (MQ=255)
                                                                                                                                            cAATCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAgg    >  1:549948/1‑148 (MQ=255)
                                                                                                                                             aaTCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGTGTCAGCATAACTTGAATGACATAGACATTACTTAATGAAATAAGAAAAGGc   >  1:1056269/1‑148 (MQ=255)
                                                                                                                                             aaTCGCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGAAATTACTTAATGAAATAAGAAAAGGc   >  1:1848219/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ACGGCACCAAACAAACCAGAACCAACAATGATATAATCGTACATAAAATCCTCAGCAAACCAGTAATTTATTATTTCTTACGAACATCAGCATGAGTGACGTAACTAAGCACTCTGTTGCAAGCAATGTTATTGCTGCACCAATCTCTTTAAAAAGAGTTGTTAGCGGAAAAATCAACAACAAACTCAACAAACCCGCAGCGATTAAAATCTTACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCA  >  CP009273/2101767‑2102056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑