Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 199,229 G→T 94.6% E61D (GAG→GAT)  lpxA → UDP‑N‑acetylglucosamine acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273199,2290GT94.6% 640.6 / 26.4 221E61D (GAG→GAT) lpxAUDP‑N‑acetylglucosamine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (8/4);  new base T (118/91);  total (126/95)
Fisher's exact test for biased strand distribution p-value = 5.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

Only 100 of 222 total aligned reads displayed.

TTGTGGAAGAGGGCGCGTCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGAGATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACG  >  CP009273/199084‑199373
                                                                                                                                                 |                                                                                                                                                
ttGTGGAAGAGGGCGCGTCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGatat                                                                                                                                                >  2:1702949/1‑148 (MQ=255)
 tgtgGAAGAGGGCGCGTCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATAtt                                                                                                                                               <  1:1728209/148‑1 (MQ=255)
  gtgGAAGAGGGCGCGTCACTTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATAttt                                                                                                                                              <  2:2855472/148‑1 (MQ=255)
     gAAGAGGGCGCGTCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATc                                                                                                                                           <  1:2013522/148‑1 (MQ=255)
       agagGGCGCGTCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAg                                                                                                                                         >  2:894747/1‑148 (MQ=255)
       agagGGCGCGTCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAg                                                                                                                                         >  1:782736/1‑148 (MQ=255)
        gagGGCGCGTCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGt                                                                                                                                        >  2:2943770/1‑148 (MQ=255)
         agGGCGCGTCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGtt                                                                                                                                       >  1:993893/1‑148 (MQ=255)
          gggCGCGTCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTc                                                                                                                                      >  2:870112/1‑148 (MQ=255)
           ggCGCGTCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCg                                                                                                                                     >  2:2797548/1‑148 (MQ=255)
             cgcgTCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGAGATTTATCAGTTCGcc                                                                                                                                   <  2:2934882/148‑1 (MQ=255)
              gcgTCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCt                                                                                                                                  <  2:607940/148‑1 (MQ=255)
                gTCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTcc                                                                                                                                <  2:107390/148‑1 (MQ=255)
                 tCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCa                                                                                                                               >  1:917879/1‑148 (MQ=255)
                  cAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCAt                                                                                                                              >  2:2181132/1‑148 (MQ=255)
                  cAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCAt                                                                                                                              >  1:2953733/1‑148 (MQ=255)
                  cAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGAGATTTATCAGTTCGCCTCCAt                                                                                                                              >  2:2091410/1‑148 (MQ=255)
                    aTTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCg                                                                                                                            >  1:6932/1‑148 (MQ=255)
                    aTTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCg                                                                                                                            >  1:45089/1‑148 (MQ=255)
                         cgcgAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGaa                                                                                                                       <  1:1204862/148‑1 (MQ=255)
                         cgcgAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGAGATTTATCAGTTCGCCTCCATCGGCGaa                                                                                                                       >  1:2137015/1‑148 (MQ=255)
                           cgAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGt                                                                                                                     >  2:250994/1‑148 (MQ=255)
                             aaCGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTa                                                                                                                   >  2:3071551/1‑148 (MQ=255)
                               cGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAACTTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAAc                                                                                                                 <  2:2712457/148‑1 (MQ=255)
                               cGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAAc                                                                                                                 <  2:936391/148‑1 (MQ=255)
                                  acacaTTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAg                                                                                                              >  1:2462321/1‑148 (MQ=255)
                                  acacaTTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAg                                                                                                              >  2:3558306/1‑148 (MQ=255)
                                     caTTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGAt                                                                                                           <  1:568932/148‑1 (MQ=255)
                                     caTTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGAt                                                                                                           <  2:571763/148‑1 (MQ=255)
                                        tGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTg                                                                                                        >  2:1610501/1‑148 (MQ=255)
                                           tCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGaaa                                                                                                     <  2:1427302/148‑1 (MQ=255)
                                           tCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGaaa                                                                                                     <  1:608801/148‑1 (MQ=255)
                                           tCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGaaa                                                                                                     >  2:516892/1‑148 (MQ=255)
                                             cTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAata                                                                                                   <  1:2174493/148‑1 (MQ=255)
                                               ttttGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATg                                                                                                 >  2:1145446/1‑148 (MQ=255)
                                               ttttGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATg                                                                                                 <  1:1350045/148‑1 (MQ=255)
                                               ttttGTATCGTTGGACCCCATGTCGAAATTGATGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGAGATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATg                                                                                                 <  1:1535789/148‑1 (MQ=255)
                                                  tGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCTCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTg                                                                                              >  1:743929/1‑148 (MQ=255)
                                                  tGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTg                                                                                              <  2:1743512/148‑1 (MQ=255)
                                                    tATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGc                                                                                            >  1:1041839/1‑148 (MQ=255)
                                                    tATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCACGATCTGAAATATGCTGGc                                                                                            >  1:1668393/1‑148 (MQ=255)
                                                       cGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATATCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGACTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGaa                                                                                         >  1:184877/1‑148 (MQ=255)
                                                            gACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGAc                                                                                    <  1:1177422/148‑1 (MQ=255)
                                                            gACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGAGATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGAc                                                                                    >  2:3358851/1‑148 (MQ=255)
                                                                ccATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCgt                                                                                <  1:2380104/148‑1 (MQ=255)
                                                                      cGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGaa                                                                          <  2:1421573/148‑1 (MQ=255)
                                                                        aaaTTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAAt                                                                        >  2:352924/1‑148 (MQ=255)
                                                                        aaaTTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAAt                                                                        <  2:3104926/148‑1 (MQ=255)
                                                                          aTTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAACATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCg                                                                      <  2:2275209/148‑1 (MQ=255)
                                                                          aTTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCg                                                                      >  1:2525787/1‑148 (MQ=255)
                                                                          aTTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCg                                                                      >  1:1352205/1‑148 (MQ=255)
                                                                          aTTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCg                                                                      >  1:1335871/1‑148 (MQ=255)
                                                                           ttGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCgg                                                                     >  1:398006/1‑148 (MQ=255)
                                                                              gTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGa                                                                  <  2:3055530/148‑1 (MQ=255)
                                                                               tGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGAt                                                                 <  1:1482293/148‑1 (MQ=255)
                                                                                gAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATc                                                                >  1:3494766/1‑148 (MQ=255)
                                                                                 aGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCg                                                               >  1:95313/1‑148 (MQ=255)
                                                                                         gTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCa                                                       >  2:1331452/1‑148 (MQ=255)
                                                                                          tACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCAt                                                      >  1:1595480/1‑148 (MQ=255)
                                                                                           aCTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCAtt                                                     <  1:103007/148‑1 (MQ=255)
                                                                                               aaaTCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGACGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTcgcg                                                 >  2:3160627/1‑148 (MQ=255)
                                                                                                aaTCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGa                                                >  1:1698633/1‑148 (MQ=255)
                                                                                                aaTCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGa                                                <  1:1279812/148‑1 (MQ=255)
                                                                                                 aTCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGAGATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGaa                                               >  1:470816/1‑148 (MQ=255)
                                                                                                  tctcACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGaaa                                              <  1:1795795/148‑1 (MQ=255)
                                                                                                   ctcACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAg                                             >  2:449380/1‑148 (MQ=255)
                                                                                                    tcACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGc                                            <  2:184877/148‑1 (MQ=255)
                                                                                                     cACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCg                                           >  2:2050852/1‑148 (MQ=255)
                                                                                                     cACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCg                                           >  2:2350246/1‑148 (MQ=255)
                                                                                                     cACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGAGATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCg                                           >  1:2929251/1‑148 (MQ=255)
                                                                                                      aCGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGt                                          <  1:2011490/148‑1 (MQ=255)
                                                                                                            tCGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCAt                                    >  2:2543806/1‑148 (MQ=255)
                                                                                                             cGTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCAtt                                   >  1:2615772/1‑148 (MQ=255)
                                                                                                              gTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTc                                  <  1:1875234/148‑1 (MQ=255)
                                                                                                              gTGAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTc                                  <  1:1355554/148‑1 (MQ=255)
                                                                                                                gAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCAt                                <  1:3437205/148‑1 (MQ=255)
                                                                                                                gAATGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCAt                                <  1:2677994/148‑1 (MQ=255)
                                                                                                                 aaTGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATc                               >  1:53398/1‑148 (MQ=255)
                                                                                                                  aTGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCg                              >  1:1825920/1‑148 (MQ=255)
                                                                                                                   tGGTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGt                             >  1:2538505/1‑148 (MQ=255)
                                                                                                                    ggTCATACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTg                            >  1:2825673/1‑148 (MQ=255)
                                                                                                                       cATACTAACATTGGCCGCTATAATGATATTTATCAGTTCGCCTACATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGca                         <  2:3427546/148‑1 (MQ=255)
                                                                                                                         tACTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGcaca                       >  2:796722/1‑148 (MQ=255)
                                                                                                                          aCTAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAACTCGGCGATCGTACCCGCATTCGCGTAAGCGTCACCCGTCCTCGTGGCACAg                      >  2:2162115/1‑148 (MQ=255)
                                                                                                                            tAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTc                    >  1:3340686/1‑148 (MQ=255)
                                                                                                                            tAAAATTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTc                    <  2:2404307/148‑1 (MQ=255)
                                                                                                                             aaaaTTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTcc                   >  2:688412/1‑148 (MQ=255)
                                                                                                                               aaTTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAg                 <  2:1021790/148‑1 (MQ=255)
                                                                                                                                aTTGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAgg                >  1:3021847/1‑148 (MQ=255)
                                                                                                                                 ttGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAggg               >  1:2098986/1‑148 (MQ=255)
                                                                                                                                  tGGCCGCGATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGc              <  1:2249039/148‑1 (MQ=255)
                                                                                                                                         gATAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGAt       <  2:2653671/148‑1 (MQ=255)
                                                                                                                                          aTAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGAtt      >  2:3476843/1‑148 (MQ=255)
                                                                                                                                          aTAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGAtt      >  2:1307401/1‑148 (MQ=255)
                                                                                                                                           tAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTg     >  2:3473500/1‑148 (MQ=255)
                                                                                                                                           tAATGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTg     >  2:1925068/1‑148 (MQ=255)
                                                                                                                                             aTGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGAc   >  1:15373/1‑148 (MQ=255)
                                                                                                                                             aTGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGAc   >  1:1382710/1‑148 (MQ=255)
                                                                                                                                              tGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACg  >  2:2453328/1‑148 (MQ=255)
                                                                                                                                              tGATATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCAGTCATCGTCTCACAGACCAGGGCGGTGGATTGACg  >  1:666769/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTGTGGAAGAGGGCGCGTCAATTGGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAGGGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAATGAGATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAACCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGTGGCACAGTCCAGGGCGGTGGATTGACG  >  CP009273/199084‑199373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑