BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,144,474	C→A	100%	intergenic (‑8/‑192)	yegI ← / → yegJ	protein kinase‑related putative non‑specific DNA‑binding protein/uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,144,474	0	C	A	99.6%	776.3 / ‑4.3	235	intergenic (‑8/‑192)	yegI/yegJ	protein kinase‑related putative non‑specific DNA‑binding protein/uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (120/114); total (121/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 236 total aligned reads displayed.

CGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACTT  >  CP009273/2144329‑2144618

cggcgTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTATCATAATATTTagc                                                                                                                                                <  1:2272139/148‑1 (MQ=255)

   cgTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCggg                                                                                                                                             <  2:546470/148‑1 (MQ=255)

     tgtgATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTa                                                                                                                                           <  1:1157874/148‑1 (MQ=255)

      gtgATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAg                                                                                                                                          >  2:1296793/1‑148 (MQ=255)

       tgATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGt                                                                                                                                         <  2:1609159/148‑1 (MQ=255)

          tAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGttat                                                                                                                                      <  2:2561206/148‑1 (MQ=255)

            aaTCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGttatat                                                                                                                                    <  2:111362/148‑1 (MQ=255)

               cTTGGCGACGCGATCGACACACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGttatattat                                                                                                                                 <  2:2391535/148‑1 (MQ=255)

                      aCGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCaa                                                                                                                          >  1:1470032/1‑148 (MQ=255)

                       cGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAAt                                                                                                                         >  2:2706835/1‑148 (MQ=255)

                         cTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGa                                                                                                                       >  1:1066968/1‑148 (MQ=255)

                         cTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGa                                                                                                                       >  2:1521839/1‑148 (MQ=255)

                            tCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGaata                                                                                                                    >  2:3186570/1‑148 (MQ=255)

                              gACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGaataat                                                                                                                  <  2:1727281/148‑1 (MQ=255)

                              gACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGaataat                                                                                                                  >  1:617712/1‑148 (MQ=255)

                               aCAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAataata                                                                                                                 <  2:777838/148‑1 (MQ=255)

                               aCAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAataata                                                                                                                 >  1:2549893/1‑148 (MQ=255)

                                cAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAAtaataa                                                                                                                >  1:102942/1‑148 (MQ=255)

                                cAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAAtaataa                                                                                                                >  1:344016/1‑148 (MQ=255)

                                   aCTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATaacaa                                                                                                             <  2:2043928/148‑1 (MQ=255)

                                        tcGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAAc                                                                                                        >  1:3488040/1‑148 (MQ=255)

                                        tcGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTAAATGAATAATAACAACTAAc                                                                                                        >  1:3099652/1‑148 (MQ=255)

                                              tCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTaa                                                                                                  >  2:227151/1‑148 (MQ=255)

                                              tCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTaa                                                                                                  >  2:2318521/1‑148 (MQ=255)

                                              tCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTaa                                                                                                  <  1:121306/148‑1 (MQ=255)

                                               cATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTaaa                                                                                                 >  1:1711809/1‑148 (MQ=255)

                                               cATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTaaa                                                                                                 <  1:3421314/148‑1 (MQ=255)

                                                  aaaCCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAatt                                                                                              >  2:1444283/1‑148 (MQ=255)

                                                   aaCCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAatta                                                                                             >  2:1129456/1‑148 (MQ=255)

                                                   aaCCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAatta                                                                                             >  2:2412476/1‑148 (MQ=255)

                                                    aCCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAattat                                                                                            <  1:373503/148‑1 (MQ=255)

                                                        cgcCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCt                                                                                        <  2:102942/148‑1 (MQ=255)

                                                        cgcCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCt                                                                                        <  2:2248284/148‑1 (MQ=255)

                                                        cgcCTTCACCACCATTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCt                                                                                        <  2:2067738/148‑1 (MQ=255)

                                                           cTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCtata                                                                                     <  2:760623/148‑1 (MQ=255)

                                                           cTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCtata                                                                                     <  1:2310972/148‑1 (MQ=255)

                                                            ttCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTatat                                                                                    <  2:3059709/148‑1 (MQ=255)

                                                             tCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATg                                                                                   >  1:2846178/1‑148 (MQ=255)

                                                               accaccTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATggg                                                                                 <  1:2907554/148‑1 (MQ=255)

                                                                ccaccTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATgggg                                                                                <  2:3172891/148‑1 (MQ=255)

                                                                   ccTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGaaa                                                                             <  1:2536881/148‑1 (MQ=255)

                                                                   ccTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGaaa                                                                             <  1:1908837/148‑1 (MQ=255)

                                                                       tGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAAccc                                                                         <  1:2819696/148‑1 (MQ=255)

                                                                        gCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCa                                                                        <  1:2015488/148‑1 (MQ=255)

                                                                            aGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCa                                                                    <  2:595134/148‑1 (MQ=255)

                                                                              ttCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAg                                                                  <  2:2509180/148‑1 (MQ=255)

                                                                              ttCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAg                                                                  <  2:1674014/148‑1 (MQ=255)

                                                                                cACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGaa                                                                <  2:2684928/148‑1 (MQ=255)

                                                                                cACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGaa                                                                >  1:3184055/1‑148 (MQ=255)

                                                                                            tGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATaa                                                    >  2:2957107/1‑148 (MQ=255)

                                                                                            tGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATaa                                                    <  2:312038/148‑1 (MQ=255)

                                                                                              gTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATg                                                  <  1:2144971/148‑1 (MQ=255)

                                                                                                cAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCa                                                >  1:2677590/1‑148 (MQ=255)

                                                                                                 aaTTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCaa                                               <  1:2686037/148‑1 (MQ=255)

                                                                                                 aaTTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCaa                                               <  2:1798424/148‑1 (MQ=255)

                                                                                                  aTTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAAt                                              >  2:1046322/1‑148 (MQ=255)

                                                                                                   ttCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAAtt                                             >  2:2767973/1‑148 (MQ=255)

                                                                                                   ttCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAAtt                                             <  2:1158520/148‑1 (MQ=255)

                                                                                                     cACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTAAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAAtttt                                           >  1:2993746/1‑148 (MQ=255)

                                                                                                       ccTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTAt                                         >  2:3213581/1‑148 (MQ=255)

                                                                                                        cTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTAta                                        >  1:954771/1‑147 (MQ=255)

                                                                                                        cTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATc                                        <  1:536951/148‑1 (MQ=255)

                                                                                                         tGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCa                                       >  2:917752/1‑148 (MQ=255)

                                                                                                         tGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCa                                       >  1:847767/1‑148 (MQ=255)

                                                                                                           tCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAgg                                     <  2:2448580/148‑1 (MQ=255)

                                                                                                           tCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAgg                                     <  2:3500728/148‑1 (MQ=255)

                                                                                                            cGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGa                                    >  2:1566826/1‑148 (MQ=255)

                                                                                                             gATGT‑AATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATa                                  <  1:1373086/148‑1 (MQ=255)

                                                                                                              aTGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATa                                  >  1:3327633/1‑148 (MQ=255)

                                                                                                               tGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAc                                 >  1:3068192/1‑148 (MQ=255)

                                                                                                                gTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGAtccg                                >  2:1971493/1‑145 (MQ=255)

                                                                                                                gTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAca                                >  1:681499/1‑148 (MQ=255)

                                                                                                                gTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAca                                >  1:2198078/1‑148 (MQ=255)

                                                                                                                 tAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAcaa                               <  2:796669/148‑1 (MQ=255)

                                                                                                                  aaaTACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAcaat                              >  2:2852249/1‑148 (MQ=255)

                                                                                                                   aaTACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAcaatt                             <  2:2126288/148‑1 (MQ=255)

                                                                                                                    aTACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAcaattc                            >  1:2943683/1‑148 (MQ=255)

                                                                                                                     tACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAcaattca                           >  2:70568/1‑148 (MQ=255)

                                                                                                                     tACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAcaattca                           <  2:2108814/148‑1 (MQ=255)

                                                                                                                     tACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAcaattca                           >  1:893420/1‑148 (MQ=255)

                                                                                                                      aCTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAcaattcaa                          >  2:65129/1‑148 (MQ=255)

                                                                                                                       cTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAcacttcaat                         >  2:1751012/1‑148 (MQ=255)

                                                                                                                          ttATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTc                      <  2:3168962/148‑1 (MQ=255)

                                                                                                                                ttGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTg                <  1:3410442/148‑1 (MQ=255)

                                                                                                                                ttGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTg                >  1:2467569/1‑148 (MQ=255)

                                                                                                                                 tGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGa               <  2:706089/148‑1 (MQ=255)

                                                                                                                                   ttttCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATa             <  2:341284/148‑1 (MQ=255)

                                                                                                                                   ttttCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATa             >  2:942386/1‑148 (MQ=255)

                                                                                                                                     ttCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAAt           >  1:433638/1‑148 (MQ=255)

                                                                                                                                     ttCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAAt           >  1:2433639/1‑148 (MQ=255)

                                                                                                                                      tCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATg          <  2:2635972/148‑1 (MQ=255)

                                                                                                                                         taataTTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATACAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCGGATAATGAtt       >  1:2125993/1‑148 (MQ=255)

                                                                                                                                         taataTTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCACTTTTATCAGGCTACAGTTCAATTTCGGCCTGATAATGAtt       >  2:2749163/1‑148 (MQ=255)

                                                                                                                                         taataTTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCTGACTGATAattatt       >  2:3197378/1‑148 (MQ=255)

                                                                                                                                           atatTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTaa     >  2:1305927/1‑148 (MQ=255)

                                                                                                                                             atTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAGAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACt   >  1:1259135/1‑148 (MQ=255)

                                                                                                                                             atTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAAATAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACt   <  2:3275990/148‑1 (MQ=255)

                                                                                                                                              tttAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCTGCCTGATAATGATTAACtt  >  1:1778765/1‑148 (MQ=255)

                                                                                                                                              tttAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACtt  <  1:78369/148‑1 (MQ=255)

                                                                                                                                              tttAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACtt  >  1:1979786/1‑148 (MQ=255)

CGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACTT  >  CP009273/2144329‑2144618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`