BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,287,829	C→A	93.4%	S185S (TCG→TCT)	ccmF ←	heme lyase, CcmF subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,287,829	0	C	A	93.4%	405.1 / 19.6	146	S185S (TCG→TCT)	ccmF	heme lyase, CcmF subunit
Reads supporting (aligned to +/- strand): ref base C (4/6); new base A (64/72); total (68/78)
Fisher's exact test for biased strand distribution p-value = 7.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 148 total aligned reads displayed.

GTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATGAAC  >  CP009273/2287684‑2287970

gTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAga                                                                                                                                             >  2:227000/1‑148 (MQ=255)

 ttCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACagag                                                                                                                                            >  1:3085577/1‑148 (MQ=255)

 ttCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACagag                                                                                                                                            >  1:2974678/1‑148 (MQ=255)

   ggTAATAGGCCCATGCGTAACCGAGCACGTTGCCGAGCTTCTGGAAGATCCACGCTGCCAGCGTCCACGGACGTGTAAAACGCGAATAAGTGCTGTCCAGACGCCCGCTCAGAAAATAAGCAATGGAAAAAGAAAACGCCACAGAGaa                                                                                                                                          <  1:3383132/147‑1 (MQ=255)

   cttAATAGGCCCATGCGTAACCGAGCACGATGCCGAGCGTCTGGAATATCCACGCTGCCAGCGTCCACGGAGGATTAAAACGCGCATATGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGAAAAAGCAAACGCCACAGAGaa                                                                                                                                          <  1:1622160/146‑1 (MQ=255)

     tAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAAc                                                                                                                                        >  1:1366490/1‑148 (MQ=255)

     tAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAAc                                                                                                                                        <  2:1891723/148‑1 (MQ=255)

      aaTAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAAcc                                                                                                                                       <  2:875308/148‑1 (MQ=255)

      aaTAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAAcc                                                                                                                                       >  2:416278/1‑148 (MQ=255)

      aaTAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAAcc                                                                                                                                       >  2:416261/1‑148 (MQ=255)

       aTAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCGGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAAccc                                                                                                                                      <  2:1030394/148‑1 (MQ=255)

        tAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCAACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCa                                                                                                                                     <  1:289468/148‑1 (MQ=255)

           gCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTACAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGt                                                                                                                                  >  2:3245893/1‑148 (MQ=255)

             ccATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTAc                                                                                                                                <  2:2779768/148‑1 (MQ=255)

             ccATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTAc                                                                                                                                <  1:2382701/148‑1 (MQ=255)

                 gCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCa                                                                                                                            >  2:466369/1‑148 (MQ=255)

                       ccGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATaaa                                                                                                                      <  2:2859662/148‑1 (MQ=255)

                       ccGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATaaa                                                                                                                      <  2:554022/148‑1 (MQ=255)

                       ccGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATaaa                                                                                                                      >  2:280357/1‑148 (MQ=255)

                          aGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCa                                                                                                                   <  2:2210254/148‑1 (MQ=255)

                            cACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCaga                                                                                                                 <  2:1300592/148‑1 (MQ=255)

                              cGATGCCGAGCGTCAGGAAGATCCTCGCTGCCAGCGTCCAGGTACGAGTAAAACGCGCAGAAGTGCGGTCCATACGCCCGCTCAGAAAATAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAgg                                                                                                               <  2:117755/148‑1 (MQ=255)

                               gATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGc                                                                                                              <  2:2581430/148‑1 (MQ=255)

                               gATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGc                                                                                                              >  2:77005/1‑148 (MQ=255)

                                   ccGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGAt                                                                                                          <  1:452383/148‑1 (MQ=255)

                                      aGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATggc                                                                                                       >  2:2647136/1‑147 (MQ=255)

                                       gCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGAATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGAAATGGAAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGaa                                                                                                      <  1:1520910/148‑1 (MQ=255)

                                          tCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAACGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGAt                                                                                                   <  1:1588091/148‑1 (MQ=255)

                                          tCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAACGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGAt                                                                                                   <  1:1588013/148‑1 (MQ=255)

                                              gAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATACAGCAGAGGCGGATGGACGagcagc                                                                                               >  2:3475233/1‑148 (MQ=255)

                                              gAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGc                                                                                               <  2:1401341/148‑1 (MQ=255)

                                              gAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGc                                                                                               >  2:470401/1‑148 (MQ=255)

                                              gAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGc                                                                                               >  1:2522493/1‑148 (MQ=255)

                                              gAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGc                                                                                               >  1:2522293/1‑148 (MQ=255)

                                              gAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGc                                                                                               >  2:3475216/1‑148 (MQ=255)

                                               aaGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGcc                                                                                              >  1:3426618/1‑148 (MQ=255)

                                                  aTCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCg                                                                                           >  2:1930915/1‑148 (MQ=255)

                                                         cTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTg                                                                                    >  1:2129903/1‑148 (MQ=255)

                                                           gCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCa                                                                                  <  2:1664549/148‑1 (MQ=255)

                                                           gCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCa                                                                                  <  2:1086493/148‑1 (MQ=255)

                                                            ccAGCGTCCACGGACGATTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCaa                                                                                 <  1:757541/148‑1 (MQ=255)

                                                            ccAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCaa                                                                                 <  1:757554/148‑1 (MQ=255)

                                                              aGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATa                                                                               <  1:2668079/148‑1 (MQ=255)

                                                                cGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGc                                                                             >  1:830369/1‑148 (MQ=255)

                                                                cGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGc                                                                             >  1:2255140/1‑148 (MQ=255)

                                                                cGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGc                                                                             >  1:2107344/1‑148 (MQ=255)

                                                                     aCGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGtt                                                                        >  2:809824/1‑148 (MQ=255)

                                                                     aCGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGtt                                                                        >  1:2902314/1‑148 (MQ=255)

                                                                         aCGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGa                                                                    <  2:3085577/148‑1 (MQ=255)

                                                                          cGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGAt                                                                   >  2:3128789/1‑148 (MQ=255)

                                                                          cGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGAt                                                                   >  2:3128715/1‑148 (MQ=255)

                                                                          cGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGAt                                                                   <  2:1720719/148‑1 (MQ=255)

                                                                           gAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATc                                                                  <  1:416278/148‑1 (MQ=255)

                                                                           gAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATc                                                                  <  1:416261/148‑1 (MQ=255)

                                                                               aaaaCGCGCATAAGTGCTGTCCAGCCGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGa                                                              <  1:3363096/148‑1 (MQ=255)

                                                                                aaaCGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGAc                                                             >  2:568402/1‑148 (MQ=255)

                                                                                aaaCGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGAc                                                             >  2:150249/1‑148 (MQ=255)

                                                                                 aaCGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGAcc                                                            >  2:704637/1‑148 (MQ=255)

                                                                                 aaCGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGAcc                                                            <  1:1964326/148‑1 (MQ=255)

                                                                                  aCGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCt                                                           >  1:989938/1‑148 (MQ=255)

                                                                                   cgcgCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAACCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCtt                                                          <  1:322388/148‑1 (MQ=255)

                                                                                      gcATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCaa                                                       <  2:2061502/148‑1 (MQ=255)

                                                                                       cATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAAt                                                      <  1:1676759/148‑1 (MQ=255)

                                                                                          aaGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCgg                                                   <  1:202494/148‑1 (MQ=255)

                                                                                            gTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGa                                                 <  2:1366490/148‑1 (MQ=255)

                                                                                            gTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGa                                                 <  1:1398765/148‑1 (MQ=255)

                                                                                             tGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGaa                                                <  2:2497598/148‑1 (MQ=255)

                                                                                                tGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGtt                                             <  1:3191137/148‑1 (MQ=255)

                                                                                                 gTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTc                                            >  1:3038795/1‑148 (MQ=255)

                                                                                                  tCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCg                                           >  2:1413807/1‑148 (MQ=255)

                                                                                                   ccAGACGCCCGCTCAGCAAAGAAGCAATGGCATAAGCAATCTCCACAGAGATACCCACGTACCCCATATAAAGCAGATGCGGTTGGAAGATCAGCCCCGGATCCTGAAATAGCGTGTTAAGATCGCGACCTTCATTCGGGTAGTTCgg                                          <  2:2316877/148‑1 (MQ=255)

                                                                                                   ccAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCgg                                          <  2:1111043/148‑1 (MQ=255)

                                                                                                      gACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCaa                                       >  2:2641787/1‑148 (MQ=255)

                                                                                                        cGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACg                                     >  2:643169/1‑148 (MQ=255)

                                                                                                          cccGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTg                                   <  1:3245893/148‑1 (MQ=255)

                                                                                                            cGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCg                                 <  2:2995187/148‑1 (MQ=255)

                                                                                                            cGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCg                                 <  2:1222846/148‑1 (MQ=255)

                                                                                                              cTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCgag                               >  1:1903453/1‑148 (MQ=255)

                                                                                                               tCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCgaga                              <  1:2092496/148‑1 (MQ=255)

                                                                                                                 aGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCgagaga                            <  2:2537166/148‑1 (MQ=255)

                                                                                                                 aGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCgagaga                            >  1:3224007/1‑148 (MQ=255)

                                                                                                                     aagaagCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACgg                        <  2:1682315/148‑1 (MQ=255)

                                                                                                                       gaagCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGt                      <  1:2820074/148‑1 (MQ=255)

                                                                                                                       gaagCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGt                      >  2:2683219/1‑148 (MQ=255)

                                                                                                                         agCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTa                    >  1:130974/1‑148 (MQ=255)

                                                                                                                         agCAATGGAAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTa                    <  1:2351395/148‑1 (MQ=255)

                                                                                                                            aaTGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTagag                 <  1:466369/148‑1 (MQ=255)

                                                                                                                             aTGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAgg                >  1:693226/1‑148 (MQ=255)

                                                                                                                             aTGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAgg                >  1:693638/1‑148 (MQ=255)

                                                                                                                             aTGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAgg                <  2:632917/148‑1 (MQ=255)

                                                                                                                              tGGCAAAAGCAAACGCCACCGAGAACCCCACGTACCCCATATAGAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGTAACAGCGGGTTAAGATCGCGCCCTTCAATCGGGAAGTTCGGCAACGTGCGTGAGAACGGGTTAGAGGt               <  1:2016280/148‑1 (MQ=255)

                                                                                                                                gCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTaa             <  1:862870/148‑1 (MQ=255)

                                                                                                                                gCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTaa             <  1:1639682/148‑1 (MQ=255)

                                                                                                                                    aaGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAagag         >  2:322484/1‑148 (MQ=255)

                                                                                                                                     aGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAgaga        <  2:2583577/148‑1 (MQ=255)

                                                                                                                                      gCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGaa       >  1:1551018/1‑148 (MQ=255)

                                                                                                                                      gCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGaa       <  1:2689341/148‑1 (MQ=255)

                                                                                                                                         aaCGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATGa    >  2:1212874/1‑148 (MQ=255)

                                                                                                                                         aaCGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATGa    <  1:1787305/148‑1 (MQ=255)

                                                                                                                                          aCGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATGaa   <  2:2974678/148‑1 (MQ=255)

GTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATGAAC  >  CP009273/2287684‑2287970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`