Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,289,043 C→A 100% W8L (TGG→TTG)  ccmD ← cytochrome c biogenesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,289,0430CA95.8% 638.5 / 17.3 216W8L (TGG→TTG) ccmDcytochrome c biogenesis protein
Reads supporting (aligned to +/- strand):  ref base C (3/6);  new base A (105/101);  total (108/108)
Fisher's exact test for biased strand distribution p-value = 3.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 220 total aligned reads displayed.

TCACGCGCCCGCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGA  >  CP009273/2288896‑2289187
                                                                                                                                                   |                                                                                                                                                
tCACGCGCCCGCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTcc                                                                                                                                                  >  1:2838541/1‑148 (MQ=255)
   cgcgCCCGCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAgg                                                                                                                                               <  1:1886998/148‑1 (MQ=255)
   cgcgCCCGCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAgg                                                                                                                                               >  1:2886727/1‑148 (MQ=255)
    gcgcCCGCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGa                                                                                                                                              <  1:2817706/148‑1 (MQ=255)
        ccGCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCa                                                                                                                                          <  1:1374049/148‑1 (MQ=255)
           cTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAAt                                                                                                                                       >  2:2911382/1‑148 (MQ=255)
            tGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATg                                                                                                                                      >  1:1864969/1‑148 (MQ=255)
               tGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAg                                                                                                                                   >  1:3250662/1‑148 (MQ=255)
                   ccacgccacgCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGt                                                                                                                               >  2:2765278/1‑148 (MQ=255)
                    cacgccacgcAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTc                                                                                                                              >  2:138115/1‑148 (MQ=255)
                       gccacgcAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCagt                                                                                                                           >  1:769554/1‑146 (MQ=255)
                       gccacgcAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCAtt                                                                                                                           >  1:769544/1‑148 (MQ=255)
                           cgcAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACg                                                                                                                       <  2:3498594/148‑1 (MQ=255)
                           cgcAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACg                                                                                                                       <  2:825751/148‑1 (MQ=255)
                                aaTTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCctc                                                                                                                  >  1:21348/1‑148 (MQ=255)
                                 aTTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCctct                                                                                                                 <  2:3369858/148‑1 (MQ=255)
                                 aTTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCctct                                                                                                                 <  2:525727/148‑1 (MQ=255)
                                  ttGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCtt                                                                                                                <  2:2199988/148‑1 (MQ=255)
                                  ttGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCtt                                                                                                                <  2:2945739/148‑1 (MQ=255)
                                     cgcgATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTc                                                                                                             >  1:145266/1‑148 (MQ=255)
                                       cgATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGACGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAg                                                                                                           >  2:1563786/1‑148 (MQ=255)
                                           gTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATc                                                                                                       <  1:424897/148‑1 (MQ=255)
                                            ttGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCa                                                                                                      <  1:3397815/148‑1 (MQ=255)
                                             tGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAg                                                                                                     <  1:2999351/148‑1 (MQ=255)
                                             tGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAg                                                                                                     <  1:1703184/148‑1 (MQ=255)
                                              tcATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGt                                                                                                    <  2:1037862/147‑1 (MQ=255)
                                              gCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGt                                                                                                    <  1:3044321/148‑1 (MQ=255)
                                              gCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGt                                                                                                    <  2:2629090/148‑1 (MQ=255)
                                                 tCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTtca                                                                                                 >  2:2138323/1‑148 (MQ=255)
                                                   aCCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTtcact                                                                                               >  1:3465597/1‑148 (MQ=255)
                                                   aCCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGACGCAAATGCAGGGGTCATTTACGGCCTCTTTGCAGTATCAGTtcact                                                                                               >  2:2497283/1‑148 (MQ=255)
                                                   aCCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTtcact                                                                                               <  1:2340592/148‑1 (MQ=255)
                                                   aCCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTtcact                                                                                               <  2:2671747/148‑1 (MQ=255)
                                                    ccGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTtcactc                                                                                              >  2:150697/1‑148 (MQ=255)
                                                    ccGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTtcactc                                                                                              >  2:2485834/1‑148 (MQ=255)
                                                    ccGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTtcactc                                                                                              <  1:3211181/148‑1 (MQ=255)
                                                     cGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTtcactca                                                                                             >  2:940074/1‑148 (MQ=255)
                                                          gCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGCCGTAACCGCCCATTGCGAAAAATACATTCAAGGAAGAAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTGCAGACACCCac                                                                                        >  2:3326471/1‑148 (MQ=255)
                                                           cACGACC‑AAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCacgg                                                                                      >  1:1961822/1‑148 (MQ=255)
                                                               aCCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCacggacg                                                                                   <  1:2503794/148‑1 (MQ=255)
                                                               aCCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGACCATTGCGAAAAATTCATTCAAGGAAGCAAATGCTGGGGTCATTTACGGCCTCTTTTCAGTATCCGTTCACTCACCCacggacg                                                                                   >  1:1624264/1‑148 (MQ=255)
                                                                ccAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCacggacgg                                                                                  >  2:3005559/1‑148 (MQ=255)
                                                                   aaaCCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGt                                                                               <  1:659485/148‑1 (MQ=255)
                                                                   aaaCCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGt                                                                               <  1:125418/148‑1 (MQ=255)
                                                                   aaaCCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGt                                                                               <  2:2973560/148‑1 (MQ=255)
                                                                    aaCCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGtt                                                                              >  2:1788838/1‑148 (MQ=255)
                                                                    aaCCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGtt                                                                              >  2:1758467/1‑148 (MQ=255)
                                                                    aaCCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGtt                                                                              >  1:354948/1‑148 (MQ=255)
                                                                    aaCCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGtt                                                                              >  1:698841/1‑148 (MQ=255)
                                                                      ccaccaGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGtttt                                                                            >  2:181528/1‑148 (MQ=255)
                                                                         ccaGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTcc                                                                         <  2:2583954/148‑1 (MQ=255)
                                                                            gCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATc                                                                      <  1:3155814/148‑1 (MQ=255)
                                                                               gAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGc                                                                   <  2:2241732/148‑1 (MQ=255)
                                                                                aaTAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCa                                                                  >  2:370762/1‑148 (MQ=255)
                                                                                  tAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCaaa                                                                <  1:1229515/148‑1 (MQ=255)
                                                                                  tAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCaaa                                                                <  2:691991/148‑1 (MQ=255)
                                                                                   aaCGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCaaaa                                                               <  1:3005559/148‑1 (MQ=255)
                                                                                     cGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATc                                                             >  1:2090401/1‑148 (MQ=255)
                                                                                      ggTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCa                                                            >  1:210406/1‑148 (MQ=255)
                                                                                         catcaCCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAAt                                                         <  2:1971956/148‑1 (MQ=255)
                                                                                         catcaCCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAAt                                                         <  1:3096751/148‑1 (MQ=255)
                                                                                          atcaCCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAAtt                                                        >  1:3310399/1‑148 (MQ=255)
                                                                                          atcaCCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAAtt                                                        >  1:3433450/1‑148 (MQ=255)
                                                                                          atcaCCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAAtt                                                        <  1:374904/148‑1 (MQ=255)
                                                                                            caccacCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATCGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTaa                                                      >  2:550268/1‑147 (MQ=255)
                                                                                                accGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgcat                                                  >  1:1387466/1‑148 (MQ=255)
                                                                                                accGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgcat                                                  <  1:2080405/148‑1 (MQ=255)
                                                                                                  cGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTGTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgcatcc                                                >  2:3288153/1‑148 (MQ=255)
                                                                                                       gccagACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgcatccgcatc                                           <  2:2803835/148‑1 (MQ=255)
                                                                                                       gccagACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTAcgcatccgcatc                                           <  2:2803415/148‑1 (MQ=255)
                                                                                                         cataCAAAAAAGGCGTAACCGCCCATTGCGAAAATTTCATTCTAGGTAGCAAATGCAGGGGTCATTTTAGTCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCTTCAGCATAATCTAATTACGCATCCGCAtcag                                         <  2:400136/145‑1 (MQ=255)
                                                                                                           gACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCAtcagcg                                       >  2:2819299/1‑148 (MQ=255)
                                                                                                               aaaaaGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCTCCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCAtcagcgtcag                                   >  1:1002953/1‑148 (MQ=255)
                                                                                                               aaaaaGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCAtcagcgtcag                                   <  1:524654/148‑1 (MQ=255)
                                                                                                                aaaaGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCAtcagcgtcagc                                  >  1:1100680/1‑148 (MQ=255)
                                                                                                                aaaaGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCAtcagcgtcagc                                  >  1:2875534/1‑148 (MQ=255)
                                                                                                                aaaaGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTGTTCCATCAGCAAAATCAAATTACGCATCCGCAtcagcgtcagc                                  >  2:1003715/1‑148 (MQ=255)
                                                                                                                   aGGCGTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTg                               <  1:1286418/148‑1 (MQ=255)
                                                                                                                       gTAACCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAg                           <  2:1864969/148‑1 (MQ=255)
                                                                                                                         aaCCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGAc                         >  1:155173/1‑148 (MQ=255)
                                                                                                                         aaCCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGAc                         <  2:21312/148‑1 (MQ=255)
                                                                                                                         aaCCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGAc                         >  1:1664/1‑148 (MQ=255)
                                                                                                                          aCCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACa                        >  2:2825768/1‑148 (MQ=255)
                                                                                                                          aCCGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACa                        >  2:3073031/1‑148 (MQ=255)
                                                                                                                           ccGCCCATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAg                       >  1:1543957/1‑148 (MQ=255)
                                                                                                                                cATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCa                  <  1:3126583/148‑1 (MQ=255)
                                                                                                                                cATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCa                  <  1:2197998/148‑1 (MQ=255)
                                                                                                                                cATTGCGAAAAATTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCa                  >  2:866573/1‑148 (MQ=255)
                                                                                                                                       aaaaaTTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTGTCAGTATCAGTTCACTCACCCACGGACGGCGTGTTTCCCTCCGCCAAATCAAAGTACGCATCCGCATCAGCGTCAGCGTGGCCGACAGGCGCCGGACGcc           >  2:2882613/1‑148 (MQ=255)
                                                                                                                                         aaaTTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCaa         >  1:2513342/1‑148 (MQ=255)
                                                                                                                                         aaaTTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCaa         >  1:65028/1‑148 (MQ=255)
                                                                                                                                          aaTTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCaaa        <  2:145266/148‑1 (MQ=255)
                                                                                                                                          aaTTCATTCAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCaaa        <  2:2069216/148‑1 (MQ=255)
                                                                                                                                           attcattcAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCaaaa       >  2:1437364/1‑148 (MQ=255)
                                                                                                                                           attcattcAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCaaaa       >  1:2033107/1‑148 (MQ=255)
                                                                                                                                            ttcattcaAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCaaaaa      >  2:899667/1‑148 (MQ=255)
                                                                                                                                             tcattcAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAAt     >  1:2823290/1‑148 (MQ=255)
                                                                                                                                             tcattcAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAAt     <  1:2765278/148‑1 (MQ=255)
                                                                                                                                              cattcAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATc    <  1:824525/148‑1 (MQ=255)
                                                                                                                                                ttcAAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGa  <  2:755505/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TCACGCGCCCGCTGTTGCGCCACGCCACGCAGAATTGCGCGATGTTGCATCACCGAGTGCACGACCAAAACCACCAGCGGAATAACGGTCATCACCACCGCCAGCCAGACAAAAAAGGCGTAACCGCCCATTGCGAAAAATTCATTCCAGGAAGCAAATGCAGGGGTCATTTACGGCCTCTTTTCAGTATCAGTTCACTCACCCACGGACGGCGTTTTTCCATCAGCAAAATCAAATTACGCATCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGA  >  CP009273/2288896‑2289187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑