Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 217,887 C→T 100% W74* (TGG→TGA)  metI ← DL‑methionine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273217,8870CT95.9% 622.2 / 13.5 193W74* (TGG→TGA) metIDL‑methionine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (6/2);  new base T (109/76);  total (115/78)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 196 total aligned reads displayed.

AAGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACAATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGC  >  CP009273/217742‑218029
                                                                                                                                                 |                                                                                                                                              
aaGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCa                                                                                                                                              >  2:391684/1‑148 (MQ=255)
 aGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAAtcattcat                                                                                                                                             <  1:1602934/148‑1 (MQ=255)
  gCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATa                                                                                                                                            >  1:3234682/1‑148 (MQ=255)
   cTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATAc                                                                                                                                           >  1:2685227/1‑148 (MQ=255)
    ttCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATAca                                                                                                                                          <  1:1542604/148‑1 (MQ=255)
     tCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATAcaa                                                                                                                                         >  1:2006175/1‑148 (MQ=255)
         ttAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATAcaagcaa                                                                                                                                     >  1:2712644/1‑148 (MQ=255)
         ttAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATAcaagcaa                                                                                                                                     >  1:1135773/1‑148 (MQ=255)
           aaCCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGa                                                                                                                                   <  1:2039516/148‑1 (MQ=255)
            aCCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGAt                                                                                                                                  >  1:1970835/1‑148 (MQ=255)
             cccGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGAtc                                                                                                                                 >  2:2801239/1‑147 (MQ=255)
             cccGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATa                                                                                                                                 <  1:260685/148‑1 (MQ=255)
             cccGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATa                                                                                                                                 <  1:260645/148‑1 (MQ=255)
                  ttGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGaa                                                                                                                            <  2:1678068/148‑1 (MQ=255)
                  ttGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGaa                                                                                                                            <  2:3456343/148‑1 (MQ=255)
                   tGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAAc                                                                                                                           >  2:2672026/1‑148 (MQ=255)
                   tGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAAc                                                                                                                           >  2:1698298/1‑148 (MQ=255)
                     ggATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACgg                                                                                                                         >  2:252781/1‑148 (MQ=255)
                      gATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACggg                                                                                                                        >  1:3332625/1‑148 (MQ=255)
                           ccAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGa                                                                                                                   <  1:317263/148‑1 (MQ=255)
                           ccAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGa                                                                                                                   >  2:637050/1‑148 (MQ=255)
                            cagcagAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGaa                                                                                                                  <  2:3537040/148‑1 (MQ=255)
                             agcagAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAAc                                                                                                                 <  2:358625/148‑1 (MQ=255)
                             agcagAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAAc                                                                                                                 >  2:1770805/1‑148 (MQ=255)
                             agcagAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCAGTCATACAAGCAAGATAATGAACGGGATGGAAc                                                                                                                 <  2:2557287/148‑1 (MQ=255)
                              gcagAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACg                                                                                                                >  2:1852195/1‑148 (MQ=255)
                                  agCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGaaa                                                                                                            >  2:2176621/1‑148 (MQ=255)
                                   gCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGaaaa                                                                                                           <  2:186258/148‑1 (MQ=255)
                                   gCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGaaaa                                                                                                           <  2:1982836/148‑1 (MQ=255)
                                    cGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAAt                                                                                                          >  1:528487/1‑148 (MQ=255)
                                       tctcGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGtt                                                                                                       >  2:1320214/1‑148 (MQ=255)
                                       tctcGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGtt                                                                                                       >  2:2195749/1‑148 (MQ=255)
                                       tctcGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGtt                                                                                                       >  2:880012/1‑148 (MQ=255)
                                          cGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTcac                                                                                                    <  2:318267/148‑1 (MQ=255)
                                            aCCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACaa                                                                                                  >  2:2369019/1‑148 (MQ=255)
                                                 aCGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCa                                                                                             >  1:3027586/1‑148 (MQ=255)
                                                 aCGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCa                                                                                             >  1:3319352/1‑148 (MQ=255)
                                                     gCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGTACGGAAACTGTTCACCATCGCAGaaa                                                                                         >  2:3459152/1‑148 (MQ=255)
                                                     gCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGaaa                                                                                         >  1:1504314/1‑148 (MQ=255)
                                                     gCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGaaa                                                                                         <  2:661628/148‑1 (MQ=255)
                                                      cAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAAc                                                                                        >  2:3260573/1‑148 (MQ=255)
                                                      cAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAAc                                                                                        >  2:811454/1‑148 (MQ=255)
                                                      cAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAAc                                                                                        >  2:1495106/1‑148 (MQ=255)
                                                         tAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGt                                                                                     <  1:161908/148‑1 (MQ=255)
                                                           aaCGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTAc                                                                                   >  2:2712392/1‑148 (MQ=255)
                                                             cGGTGCTGCACCAACGGGTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGa                                                                                 <  2:2268370/148‑1 (MQ=255)
                                                               gTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATa                                                                               <  1:1031861/148‑1 (MQ=255)
                                                                tgctgcACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATAc                                                                              <  1:677092/148‑1 (MQ=255)
                                                                  ctgcACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGTTACGATACAg                                                                            >  1:574563/1‑148 (MQ=255)
                                                                  ctgcACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAg                                                                            >  1:276781/1‑148 (MQ=255)
                                                                    gcACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCt                                                                          <  2:1765640/148‑1 (MQ=255)
                                                                    gcACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACAATCGCAGAAACGGTACGATACAGCt                                                                          <  1:255314/148‑1 (MQ=255)
                                                                     cACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCtt                                                                         <  2:1816790/148‑1 (MQ=255)
                                                                      aCCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTc                                                                        <  2:399209/148‑1 (MQ=255)
                                                                      aCCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTc                                                                        <  2:398611/148‑1 (MQ=255)
                                                                        cAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTcgc                                                                      <  1:2470822/148‑1 (MQ=255)
                                                                        cAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTcgc                                                                      <  1:687105/148‑1 (MQ=255)
                                                                         aaCGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTcgcg                                                                     <  1:1408824/148‑1 (MQ=255)
                                                                          aCGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGt                                                                    >  1:1781436/1‑148 (MQ=255)
                                                                          aCGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGt                                                                    >  1:2124218/1‑148 (MQ=255)
                                                                           cGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGtt                                                                   <  1:1315100/148‑1 (MQ=255)
                                                                                aaCGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATACTGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCaa                                                              <  1:2238687/148‑1 (MQ=255)
                                                                                  cGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCaata                                                            >  1:2010327/1‑148 (MQ=255)
                                                                                   ggAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCaataa                                                           >  2:1505366/1‑148 (MQ=255)
                                                                                   ggAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCaataa                                                           <  2:30118/148‑1 (MQ=255)
                                                                                    gAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCaataat                                                          <  1:2307547/148‑1 (MQ=255)
                                                                                     aaCAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAAAGAACGGGATGGAACGGAAAATGTACACAAACTCAGAAAAGGTAAGATACAGCTTCGCGTTAGCaataata                                                         >  1:1549696/1‑147 (MQ=255)
                                                                                       cAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTg                                                       >  1:3543641/1‑148 (MQ=255)
                                                                                       cAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTg                                                       >  2:294510/1‑148 (MQ=255)
                                                                                              tgcctgcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGa                                                <  1:3000632/148‑1 (MQ=255)
                                                                                               gcctgcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGAc                                               >  1:1832243/1‑148 (MQ=255)
                                                                                               gcctgcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGAc                                               >  2:2687416/1‑148 (MQ=255)
                                                                                                  tgcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGAcgcg                                            >  1:2745237/1‑148 (MQ=255)
                                                                                                   gcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGt                                           >  2:2489918/1‑148 (MQ=255)
                                                                                                    cAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCACTAATTTGCCCCGCACGCGTg                                          >  2:2192406/1‑148 (MQ=255)
                                                                                                    cAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTg                                          <  2:3370581/148‑1 (MQ=255)
                                                                                                       aCCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACa                                       >  1:2966418/1‑148 (MQ=255)
                                                                                                        ccAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGATTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACAt                                      <  1:1698298/148‑1 (MQ=255)
                                                                                                           aTCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATaaa                                   >  2:2878090/1‑148 (MQ=255)
                                                                                                            tCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATCATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAg                                  >  2:775477/1‑148 (MQ=255)
                                                                                                            tCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAg                                  >  2:208032/1‑148 (MQ=255)
                                                                                                            tCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAg                                  >  1:471583/1‑148 (MQ=255)
                                                                                                                tGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGa                              <  1:677440/148‑1 (MQ=255)
                                                                                                                tGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGa                              <  1:3181163/148‑1 (MQ=255)
                                                                                                                 gTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGaa                             >  2:638304/1‑148 (MQ=255)
                                                                                                                 gTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGaa                             >  2:638162/1‑148 (MQ=255)
                                                                                                                  tACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAAc                            >  1:2137698/1‑148 (MQ=255)
                                                                                                                        cAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAAc                      <  1:1116575/148‑1 (MQ=255)
                                                                                                                               gcgGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGa               >  2:231173/1‑148 (MQ=255)
                                                                                                                                cgGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACAATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGAc              >  1:997574/1‑148 (MQ=255)
                                                                                                                                   gTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCaa           <  2:3300718/148‑1 (MQ=255)
                                                                                                                                     aaaCGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATc         <  2:542813/148‑1 (MQ=255)
                                                                                                                                      aaCGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATca        <  2:1073408/148‑1 (MQ=255)
                                                                                                                                      aaCGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACAATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATca        >  1:23688/1‑148 (MQ=255)
                                                                                                                                       aCGGAATAATTCATTCAAGAAAGATAATGAACGGGATGGAACGGATATTGTTCACCATCGCAGTAACTGTACGATTCTGCTTCGCGTTAGAAATAATTTGCCCCGGACGCGTGACATATAGCTTAACGCCAACCGTCAGACCAATcac       <  2:1549696/148‑1 (MQ=255)
                                                                                                                                        cGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATcaca      >  1:100094/1‑148 (MQ=255)
                                                                                                                                           aaTCATTCATACAAGCAAGATAATGAACTGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTAAGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAg   >  1:968155/1‑148 (MQ=255)
                                                                                                                                           aaTCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAg   >  2:1151003/1‑148 (MQ=255)
                                                                                                                                            atcattCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGc  >  1:2844974/1‑148 (MQ=255)
                                                                                                                                            atcattCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGc  <  1:391684/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
AAGCTTCAATTAACCCGGTTGGGATCTCCAGCAGAGCGTTCTCGACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACAATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGC  >  CP009273/217742‑218029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑