Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,302,843 C→A 6.5% E348* (GAA→TAA)  ada ← fused DNA‑binding transcriptional dual regulator/O6‑methylguanine‑DNA methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,302,8430CA 6.5% 800.0 / 19.1 215E348* (GAA→TAA) adafused DNA‑binding transcriptional dual regulator/O6‑methylguanine‑DNA methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (110/91);  new base A (9/5);  total (119/96)
Fisher's exact test for biased strand distribution p-value = 5.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 225 total aligned reads displayed.

CTGGCAACGTCATTAATATCGCGGATCAGTTGCTCCGCAGCGTTAAAAGCAAAACGCCGTAAAATTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGT  >  CP009273/2302696‑2302988
                                                                                                                                                   |                                                                                                                                                 
  ggcAACGTCATTAATATCGCGGATCAGTTGCTCCGCAGCGTTAAAAGCAAAACGCCGTAAAATTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTcgc                                                                                                                                                 >  1:1144620/1‑148 (MQ=255)
  ggcAACGTCATTAATATCGCGGATCAGTTGCTCCGCAGCGTTAAAAGCAAAACGCCGTAAAATTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTcgc                                                                                                                                                 <  1:3510730/148‑1 (MQ=255)
      aCGTCATTAATATCGCGGATCAGTTGCTCCGCAGCGTTAAAAGCAAAACGCCGTAAAATTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCgcggcg                                                                                                                                             >  2:1065306/1‑148 (MQ=255)
       cGTCATTAATATCGCGGATCAGTTGCTCCGCAGCGTTAAAAGCAAAACGCCGTAAAATTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGgcgc                                                                                                                                            <  1:2414757/148‑1 (MQ=255)
         tCATTAATATCGCGGATCAGTTGCTCCGCAGCGTTAAAAGCAAAACGCCGTAAAATTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCgcag                                                                                                                                          >  2:1950003/1‑148 (MQ=255)
             tAATATCGCGGATCAGTTGCTCCGCAGCGTTAAAAGCAAAACGCCGTAAAATTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGt                                                                                                                                      >  1:1635288/1‑148 (MQ=255)
                tatCGCGGATCAGTTGCTCCGCAGCGTTAAAAGCAAAACGCCGTAAAATTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTgc                                                                                                                                   >  2:2829862/1‑148 (MQ=255)
                   cgcgGATCAGTTGCTCCGCAGCGTTAAAAGCAAAACGCCGTAAAATTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCt                                                                                                                                <  2:2379769/148‑1 (MQ=255)
                          cAGTTGCTCCGCAGCGTTAAAAGCAAAACGCCGTAAAATTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTAcgcg                                                                                                                         >  1:1342766/1‑148 (MQ=255)
                          cAGTTGCTCCGCAGCGTTAAAAGCAAAACGCCGTAAAATTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTAcgcg                                                                                                                         <  2:753033/148‑1 (MQ=255)
                             ttGCTCCGCAGCGTTAAAAGCAAAACGCCGTAAAATTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGaca                                                                                                                      >  1:3519762/1‑148 (MQ=255)
                             ttGCTCCGCAGCGTTAAAAGCAAAACGCCGTAAAATTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGaca                                                                                                                      >  2:1115558/1‑148 (MQ=255)
                               gCTCCGCAGCGTTAAAAGCAAAACGCCGTAAAATTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACg                                                                                                                    >  2:2219185/1‑148 (MQ=255)
                                cTCCGCAGCGTTAAAAGCAAAACGCCGTAAAATTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGc                                                                                                                   >  1:872582/1‑148 (MQ=255)
                                 tCCGCAGCGTTAAAAGCAAAACGCCGTAAAATTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGcc                                                                                                                  <  1:2416210/148‑1 (MQ=255)
                                        cGTTAAAAGCAAAACGCCGTAAAATTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCgg                                                                                                           >  1:2683400/1‑148 (MQ=255)
                                        cGTTAAAAGCAAAACGCCGTAAAATTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCgg                                                                                                           >  2:1829703/1‑148 (MQ=255)
                                        cGTTAAAAGCAAAACGCCGTAAAATTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCgg                                                                                                           >  1:1442026/1‑148 (MQ=255)
                                         gTTAAAAGCAAAACGCCGTAAAATTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGt                                                                                                          <  2:2696930/148‑1 (MQ=255)
                                          ttAAAAGCAAAACGCCGTAAAATTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTa                                                                                                         <  1:412145/148‑1 (MQ=255)
                                           tAAAAGCAAAACGCCGTAAAATTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTaa                                                                                                        >  1:3393873/1‑148 (MQ=255)
                                            aaaaGCAAAACGCCGTAAAATTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAAc                                                                                                       <  1:91561/148‑1 (MQ=255)
                                               aGCAAAACGCCGTAAAATTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATGTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCgg                                                                                                    >  1:1820937/1‑148 (MQ=255)
                                                  aaaaCGCCGTAAAATTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCTGCAAACAGATCCAAATTTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGATTTACGCGACACGCCCCAGCGGTAACCGGaaa                                                                                                 <  1:105162/148‑1 (MQ=255)
                                                     aCGCCGTAAAATTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAgtg                                                                                              >  1:783081/1‑148 (MQ=255)
                                                     aCGCCGTAAAATTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAgtg                                                                                              <  2:2567093/148‑1 (MQ=255)
                                                        ccGTAAAATTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTAGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGc                                                                                           <  2:1611110/148‑1 (MQ=255)
                                                           tAAAATTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCAt                                                                                        <  1:3465063/148‑1 (MQ=255)
                                                              aaTTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATcac                                                                                     >  1:595057/1‑148 (MQ=255)
                                                              aaTTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATcac                                                                                     >  1:44462/1‑148 (MQ=255)
                                                               aTTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATcacc                                                                                    >  2:1282372/1‑148 (MQ=255)
                                                                ttACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATcacca                                                                                   <  2:2071547/148‑1 (MQ=255)
                                                                  aCCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACg                                                                                 >  1:2463435/1‑148 (MQ=255)
                                                                   ccGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACgg                                                                                <  2:683606/148‑1 (MQ=255)
                                                                     gCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGac                                                                              <  2:1442553/148‑1 (MQ=255)
                                                                           gccgccAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCg                                                                        <  1:3372940/148‑1 (MQ=255)
                                                                           gccgccAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCg                                                                        <  1:2761122/148‑1 (MQ=255)
                                                                            ccgccAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGa                                                                       >  1:2680641/1‑148 (MQ=255)
                                                                            ccgccAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGa                                                                       <  1:2993091/148‑1 (MQ=255)
                                                                              gccAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATg                                                                     <  2:2409828/148‑1 (MQ=255)
                                                                              gccAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATg                                                                     <  2:2246116/148‑1 (MQ=255)
                                                                                cAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTAGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGAc                                                                   >  2:17357/1‑148 (MQ=255)
                                                                                cAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTAGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGAc                                                                   <  1:2286279/148‑1 (MQ=255)
                                                                                cAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTAGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGAc                                                                   >  2:1817677/1‑148 (MQ=255)
                                                                                 aGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACa                                                                  <  2:2455973/148‑1 (MQ=255)
                                                                                   tGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAgg                                                                <  1:2607897/148‑1 (MQ=255)
                                                                                   tGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAgg                                                                >  2:723104/1‑148 (MQ=255)
                                                                                       tctTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGtat                                                            <  2:3412455/148‑1 (MQ=255)
                                                                                       tctTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGtat                                                            >  1:2749443/1‑148 (MQ=255)
                                                                                        ctTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGtatt                                                           <  2:2014175/148‑1 (MQ=255)
                                                                                        ctTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGtatt                                                           <  1:3388001/148‑1 (MQ=255)
                                                                                         ttGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGtatta                                                          <  2:508090/148‑1 (MQ=255)
                                                                                               cGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAg                                                    <  1:350042/148‑1 (MQ=255)
                                                                                               cGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAg                                                    >  1:1383893/1‑148 (MQ=255)
                                                                                               cGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTAGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAg                                                    >  2:1868997/1‑148 (MQ=255)
                                                                                                 gTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGcc                                                  >  1:765703/1‑148 (MQ=255)
                                                                                                       cATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCttg                                            <  1:931460/148‑1 (MQ=255)
                                                                                                        aTCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCttgt                                           <  1:743497/148‑1 (MQ=255)
                                                                                                         tCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCttgtt                                          >  1:1406643/1‑148 (MQ=255)
                                                                                                         tCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCttgtt                                          >  2:2627998/1‑148 (MQ=255)
                                                                                                         tCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCttgtt                                          >  2:862112/1‑148 (MQ=255)
                                                                                                           ggCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTgg                                        >  1:3430871/1‑148 (MQ=255)
                                                                                                            gCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTggc                                       <  1:2617667/148‑1 (MQ=255)
                                                                                                            gCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCACAGCGGTAACCGGAAAGTGTGCCATCACCACGTACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTggc                                       <  1:174986/148‑1 (MQ=255)
                                                                                                               aaCAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTggcggc                                    >  2:112848/1‑148 (MQ=255)
                                                                                                                aCAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGca                                   >  1:3202467/1‑148 (MQ=255)
                                                                                                                aCAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGca                                   <  2:407626/148‑1 (MQ=255)
                                                                                                                aCAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGca                                   >  1:385720/1‑148 (MQ=255)
                                                                                                                    aTCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAgg                               >  2:1667263/1‑148 (MQ=255)
                                                                                                                     tCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGgc                              >  2:3525200/1‑148 (MQ=255)
                                                                                                                       cAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGgcgc                            >  2:3509705/1‑148 (MQ=255)
                                                                                                                         aCATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTg                          >  1:814191/1‑148 (MQ=255)
                                                                                                                         aCATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTg                          >  2:426427/1‑148 (MQ=255)
                                                                                                                          cATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTgg                         <  1:1234953/148‑1 (MQ=255)
                                                                                                                            ttACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCa                       <  1:2709904/148‑1 (MQ=255)
                                                                                                                             tACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCaa                      >  2:1679837/1‑148 (MQ=255)
                                                                                                                              aCCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAAc                     >  2:2183899/1‑148 (MQ=255)
                                                                                                                                ctctCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACgg                   >  2:1092607/1‑148 (MQ=255)
                                                                                                                                ctctCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACgg                   >  2:1093073/1‑148 (MQ=255)
                                                                                                                                 tctcCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGc                  >  1:57165/1‑148 (MQ=255)
                                                                                                                                 tctcCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGc                  <  1:3269744/148‑1 (MQ=255)
                                                                                                                                   tcctcATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGccc                >  1:704865/1‑148 (MQ=255)
                                                                                                                                    cctcATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCg               >  2:2354699/1‑148 (MQ=255)
                                                                                                                                    cctcATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCg               >  2:2354347/1‑148 (MQ=255)
                                                                                                                                      tcATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTa             >  1:2510714/1‑148 (MQ=255)
                                                                                                                                        aTTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTAcc           >  2:1661190/1‑148 (MQ=255)
                                                                                                                                        aTTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTAcc           >  2:1672405/1‑148 (MQ=255)
                                                                                                                                         ttttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCg          <  1:2091119/148‑1 (MQ=255)
                                                                                                                                          tttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGc         >  1:1972276/1‑148 (MQ=255)
                                                                                                                                          tttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGc         <  1:2895808/148‑1 (MQ=255)
                                                                                                                                          tttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGc         <  1:1161606/148‑1 (MQ=255)
                                                                                                                                          tttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGc         <  1:3071398/148‑1 (MQ=255)
                                                                                                                                          tttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGc         <  2:1189220/148‑1 (MQ=255)
                                                                                                                                           ttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCt        <  1:916125/148‑1 (MQ=255)
                                                                                                                                           ttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCt        <  2:2989173/148‑1 (MQ=255)
                                                                                                                                               gCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCg    <  1:2976345/148‑1 (MQ=255)
                                                                                                                                                cTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCgg   <  2:3206962/148‑1 (MQ=255)
                                                                                                                                                cTTAGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCgg   >  1:160491/1‑148 (MQ=255)
                                                                                                                                                cTTAGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCgg   >  1:698387/1‑148 (MQ=255)
                                                                                                                                                 ttCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGt  <  1:1950003/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CTGGCAACGTCATTAATATCGCGGATCAGTTGCTCCGCAGCGTTAAAAGCAAAACGCCGTAAAATTACCGCACCAGCCGCCAGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCACCACGGACCACCCGATGACAGGGTATTATGATAGCCAGCTTGTTGGCGGCACAGGCGCTGGCAACGGCCCGTACCGCTTTCGGT  >  CP009273/2302696‑2302988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑