Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 217,932 A→C 100% I59M (ATT→ATG)  metI ← DL‑methionine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273217,9320AC95.5% 803.3 / 9.4 207I59M (ATT→ATG) metIDL‑methionine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (7/3);  new base C (113/84);  total (120/87)
Fisher's exact test for biased strand distribution p-value = 5.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 208 total aligned reads displayed.

ACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACAATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCATACGCC  >  CP009273/217786‑218075
                                                                                                                                                  |                                                                                                                                               
     aCGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCa                                                                                                                                           >  1:3319352/1‑148 (MQ=255)
     aCGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCa                                                                                                                                           >  1:3027586/1‑148 (MQ=255)
         gCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGTACGGAAACTGTTCACCATCGCAGaaa                                                                                                                                       >  2:3459152/1‑148 (MQ=255)
         gCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGaaa                                                                                                                                       <  2:661628/148‑1 (MQ=255)
         gCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACAATCGCAGaaa                                                                                                                                       >  2:255314/1‑148 (MQ=255)
          cAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAAc                                                                                                                                      >  2:1495106/1‑148 (MQ=255)
          cAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAAc                                                                                                                                      >  2:811454/1‑148 (MQ=255)
            aTAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACgg                                                                                                                                    >  1:1063087/1‑148 (MQ=255)
               aaCGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTAc                                                                                                                                 >  1:3493296/1‑148 (MQ=255)
               aaCGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTAc                                                                                                                                 >  2:2712392/1‑148 (MQ=255)
                   gTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATa                                                                                                                             <  1:2006893/148‑1 (MQ=255)
                   gTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATa                                                                                                                             <  1:2431387/148‑1 (MQ=255)
                      ctgcACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAg                                                                                                                          >  1:276781/1‑148 (MQ=255)
                       tgcTCCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGc                                                                                                                         <  1:573416/148‑1 (MQ=255)
                        gcACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCt                                                                                                                        <  2:1765301/148‑1 (MQ=255)
                        gcACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCt                                                                                                                        <  1:1386983/148‑1 (MQ=255)
                        gcACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACAATCGCAGAAACGGTACGATACAGCt                                                                                                                        <  1:255314/148‑1 (MQ=255)
                          aCCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTc                                                                                                                      <  2:398611/148‑1 (MQ=255)
                          aCCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTc                                                                                                                      <  2:399209/148‑1 (MQ=255)
                              aCGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGt                                                                                                                  >  1:1781436/1‑148 (MQ=255)
                              aCGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGt                                                                                                                  >  1:2124617/1‑148 (MQ=255)
                               cGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGtt                                                                                                                 <  1:1315100/148‑1 (MQ=255)
                                ggTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTa                                                                                                                <  1:1524110/148‑1 (MQ=255)
                                    aaCGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATACTGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCaa                                                                                                            <  1:2238687/148‑1 (MQ=255)
                                      cGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCaata                                                                                                          >  1:511147/1‑148 (MQ=255)
                                      cGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCaata                                                                                                          <  1:617154/148‑1 (MQ=255)
                                       ggAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCaataa                                                                                                         >  2:1505366/1‑148 (MQ=255)
                                       ggAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCaataa                                                                                                         <  2:30118/148‑1 (MQ=255)
                                        gAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCaataat                                                                                                        <  1:2307547/148‑1 (MQ=255)
                                        gAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCaataat                                                                                                        <  2:2664136/148‑1 (MQ=255)
                                         aaCAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAAAGAACGGGATGGAACGGAAAATGTACACAAACTCAGAAAAGGTAAGATACAGCTTCGCGTTAGCaataata                                                                                                       >  1:1549696/1‑147 (MQ=255)
                                           cAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTg                                                                                                     >  2:294510/1‑148 (MQ=255)
                                                  tgcctgcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGa                                                                                              <  1:3000632/148‑1 (MQ=255)
                                                   gcctgcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGAc                                                                                             >  1:1832243/1‑148 (MQ=255)
                                                      tgcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGAcgcg                                                                                          >  1:2745237/1‑148 (MQ=255)
                                                       gcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGt                                                                                         >  2:2489918/1‑148 (MQ=255)
                                                        cAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTg                                                                                        >  2:2192411/1‑148 (MQ=255)
                                                        cAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTg                                                                                        >  2:2192273/1‑148 (MQ=255)
                                                        cAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTg                                                                                        <  2:3370581/148‑1 (MQ=255)
                                                         aaaCCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGa                                                                                       <  2:562618/148‑1 (MQ=255)
                                                           aCCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACa                                                                                     >  1:2966418/1‑148 (MQ=255)
                                                           aCCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACAATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACa                                                                                     >  1:972061/1‑148 (MQ=255)
                                                            ccAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGATTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACAt                                                                                    <  1:1698292/148‑1 (MQ=255)
                                                               aTCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATaaa                                                                                 >  2:2878090/1‑148 (MQ=255)
                                                                tCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATCATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAg                                                                                >  2:775477/1‑148 (MQ=255)
                                                                tCGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAg                                                                                >  2:208032/1‑148 (MQ=255)
                                                                 cGATGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGc                                                                               <  1:1607585/148‑1 (MQ=255)
                                                                    tGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGa                                                                            <  1:3181157/148‑1 (MQ=255)
                                                                    tGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGa                                                                            <  1:677453/148‑1 (MQ=255)
                                                                    tGTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGa                                                                            <  1:677432/148‑1 (MQ=255)
                                                                     gTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGaa                                                                           >  2:638162/1‑148 (MQ=255)
                                                                     gTACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGaa                                                                           >  2:638304/1‑148 (MQ=255)
                                                                      tACCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAAc                                                                          >  1:2137698/1‑148 (MQ=255)
                                                                       aCCGACAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACg                                                                         <  1:3555634/148‑1 (MQ=255)
                                                                            cAATAACGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAAc                                                                    <  1:1116575/148‑1 (MQ=255)
                                                                                 aCGCGGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCa                                                               >  2:1368399/1‑148 (MQ=255)
                                                                                  cgcgGTTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACTGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAg                                                              <  1:320440/148‑1 (MQ=255)
                                                                                   gcgGGTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGa                                                             >  2:231165/1‑148 (MQ=255)
                                                                                       gTAAACGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCaa                                                         <  2:3300718/148‑1 (MQ=255)
                                                                                         aaaCGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATc                                                       >  2:1770335/1‑148 (MQ=255)
                                                                                          aaCGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATca                                                      <  2:1073408/148‑1 (MQ=255)
                                                                                          aaCGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACAATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATca                                                      >  1:23688/1‑148 (MQ=255)
                                                                                           aCGGAATAATTCATTCAAGAAAGATAATGAACGGGATGGAACGGATATTGTTCACCATCGCAGTAACTGTACGATTCTGCTTCGCGTTAGAAATAATTTGCCCCGGACGCGTGACATATAGCTTAACGCCAACCGTCAGACCAATcac                                                     <  2:1549696/148‑1 (MQ=255)
                                                                                            cGGAATCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATcaca                                                    >  1:100094/1‑148 (MQ=255)
                                                                                               aaTCATTCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAg                                                 >  2:1151003/1‑148 (MQ=255)
                                                                                                   attcATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCaa                                             >  2:1891488/4‑148 (MQ=255)
                                                                                                    ttcATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCaaa                                            >  2:2041967/3‑148 (MQ=255)
                                                                                                        tACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCaaaaaaa                                        <  2:528487/148‑1 (MQ=255)
                                                                                                        tACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCaaaaaaa                                        >  1:2014592/1‑148 (MQ=255)
                                                                                                         aCAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAAc                                       >  2:3285192/1‑148 (MQ=255)
                                                                                                         aCAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAAc                                       <  1:1320214/148‑1 (MQ=255)
                                                                                                         aCAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAAc                                       <  1:2672053/148‑1 (MQ=255)
                                                                                                             gcaagATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGa                                   >  1:2844584/1‑148 (MQ=255)
                                                                                                              caagATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGAt                                  >  2:2330853/1‑148 (MQ=255)
                                                                                                              caagATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGAt                                  <  1:2489918/148‑1 (MQ=255)
                                                                                                                 gATAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGATAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACg                               >  2:3256971/1‑148 (MQ=255)
                                                                                                                  aTAATGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGa                              >  2:2931577/1‑148 (MQ=255)
                                                                                                                     aTGAACGGTATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAgg                           <  1:637087/148‑1 (MQ=255)
                                                                                                                     aTGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAgg                           <  1:461854/148‑1 (MQ=255)
                                                                                                                     aTGAACGGGATGGAACGGAAAATGTTCACAATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAATCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAgg                           >  1:729375/1‑148 (MQ=255)
                                                                                                                      tGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGt                          >  2:2363743/1‑148 (MQ=255)
                                                                                                                      tGAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGt                          >  2:2264226/1‑148 (MQ=255)
                                                                                                                       gAACGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTc                         <  1:339502/148‑1 (MQ=255)
                                                                                                                         aCGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCAt                       >  2:1016468/1‑148 (MQ=255)
                                                                                                                         aCGGGATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCAt                       >  2:1016565/1‑148 (MQ=255)
                                                                                                                           gggATGGAACGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTg                     >  2:835470/1‑148 (MQ=255)
                                                                                                                                  aaCGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGt              <  2:2398218/148‑1 (MQ=255)
                                                                                                                                  aaCGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGt              <  1:2620959/148‑1 (MQ=255)
                                                                                                                                  aaCGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGt              >  1:2115561/1‑148 (MQ=255)
                                                                                                                                   aCGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGtt             <  1:2693888/148‑1 (MQ=255)
                                                                                                                                    cGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGttt            <  1:2195749/148‑1 (MQ=255)
                                                                                                                                    cGGAAAATGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGttt            >  1:1485391/1‑148 (MQ=255)
                                                                                                                                         aaTGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCAt       >  1:410635/1‑148 (MQ=255)
                                                                                                                                          aTGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCATa      >  2:728096/1‑148 (MQ=255)
                                                                                                                                          aTGTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCATa      <  2:2685252/148‑1 (MQ=255)
                                                                                                                                            gTTCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCATACg    >  1:836640/1‑148 (MQ=255)
                                                                                                                                             ttCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCATACGc   >  2:2050133/1‑148 (MQ=255)
                                                                                                                                             ttCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCATACGc   >  1:3102258/1‑148 (MQ=255)
                                                                                                                                              tCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCATACGcc  <  2:3234682/148‑1 (MQ=255)
                                                                                                                                              tCACCATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCATACGcc  <  1:2047296/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
ACCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGGGATGGAACGGAAAATGTTCACAATCGCAGAAACGGTACGATACAGCTTCGCGTTAGCAATAATTTGCCCCGGACGCGTGACATAAAGCAGAACGCCAACCGGCAGACCAATCACAAAGCCAAAAAAACCGGATACGAAGGTCATTGCCAGCGTTTCCCATACGCC  >  CP009273/217786‑218075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑