Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,376,959 C→T 100% N256N (AAC→AAT)  elaD → protease, capable of cleaving an AMC‑ubiquitin model substrate

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,376,9590CT98.1% 818.5 / 4.6 258N256N (AAC→AAT) elaDprotease, capable of cleaving an AMC‑ubiquitin model substrate
Reads supporting (aligned to +/- strand):  ref base C (1/4);  new base T (125/128);  total (126/132)
Fisher's exact test for biased strand distribution p-value = 3.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 260 total aligned reads displayed.

ATGAAAATATTAAGAGCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACATGCCGAT  >  CP009273/2376814‑2377103
                                                                                                                                                 |                                                                                                                                                
aTGAAAATATTAAGAGCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTc                                                                                                                                                <  1:1735508/148‑1 (MQ=255)
 tGAAAATATTAAGAGCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATattcaattca                                                                                                                                               >  2:52110/1‑148 (MQ=255)
   aaaaTATTAAGAGCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCAtt                                                                                                                                             >  2:1513880/1‑148 (MQ=255)
    aaaTATTAAGAGCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTg                                                                                                                                            <  1:2600374/148‑1 (MQ=255)
     aaTATTAAGAGCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGc                                                                                                                                           >  1:1407745/1‑148 (MQ=255)
      atatTAAGAGCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCg                                                                                                                                          >  2:2889298/1‑148 (MQ=255)
       tatTAAGAGCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGt                                                                                                                                         >  1:1037253/1‑148 (MQ=255)
       tatTAAGAGCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGt                                                                                                                                         >  2:3477800/1‑148 (MQ=255)
         ttAAGAGCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGc                                                                                                                                       >  1:2871447/1‑148 (MQ=255)
          tAAGAGCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCa                                                                                                                                      >  2:401270/1‑148 (MQ=255)
              agCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAg                                                                                                                                  >  1:3015502/1‑148 (MQ=255)
                 gATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCt                                                                                                                               <  1:3101501/148‑1 (MQ=255)
                  aTCCATCATGGCCGAATGAAGTCCAGTTAGTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTa                                                                                                                              <  2:3128635/148‑1 (MQ=255)
                   tCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTAt                                                                                                                             >  2:2919997/1‑148 (MQ=255)
                   tCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTAt                                                                                                                             >  2:1430491/1‑148 (MQ=255)
                         atGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATaaa                                                                                                                       <  1:828654/148‑1 (MQ=255)
                           ggCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGa                                                                                                                     >  2:1201241/1‑148 (MQ=255)
                                 aTGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTc                                                                                                               <  1:691520/148‑1 (MQ=255)
                                 aTGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTc                                                                                                               <  2:1957892/148‑1 (MQ=255)
                                 aTGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTc                                                                                                               >  1:290323/1‑148 (MQ=255)
                                 aTGAAGTCCAGTTATTCCACATAAATACTGGCGTACACTGGATATTATTTTCGATACATAAAATAGTAAATTAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTTTGATAAATAAAATTc                                                                                                               <  1:554905/148‑1 (MQ=255)
                                  tGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCa                                                                                                              <  1:2153597/148‑1 (MQ=255)
                                       tCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTaa                                                                                                         <  1:812908/148‑1 (MQ=255)
                                       tCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTaa                                                                                                         >  2:77426/1‑148 (MQ=255)
                                         cAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGc                                                                                                       <  1:3145605/148‑1 (MQ=255)
                                          aGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCg                                                                                                      <  2:16691/148‑1 (MQ=255)
                                            ttATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCgtg                                                                                                    <  2:3002466/148‑1 (MQ=255)
                                             tATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCgtgt                                                                                                   >  1:1184555/1‑148 (MQ=255)
                                             tATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCgtgt                                                                                                   <  1:1623906/148‑1 (MQ=255)
                                                tCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCAt                                                                                                >  1:1893268/1‑148 (MQ=255)
                                                tCCCCATAAAGACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCAt                                                                                                <  1:27784/148‑1 (MQ=255)
                                                  cccATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTAGGAGAAAGAAAATTCACTTAAGCGTGTCATTa                                                                                              >  1:3332306/1‑148 (MQ=255)
                                                   ccATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATTCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTaa                                                                                             >  1:157619/1‑148 (MQ=255)
                                                   ccATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTaa                                                                                             <  1:113347/148‑1 (MQ=255)
                                                    cATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAAt                                                                                            <  1:2310260/148‑1 (MQ=255)
                                                     aTAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATa                                                                                           >  2:3197745/1‑148 (MQ=255)
                                                      tAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAg                                                                                          <  1:124228/148‑1 (MQ=255)
                                                      tAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAg                                                                                          >  1:998274/1‑148 (MQ=255)
                                                       aaaTACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGt                                                                                         >  2:2877197/1‑148 (MQ=255)
                                                             tGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAAt                                                                                   <  2:3507856/148‑1 (MQ=255)
                                                                   acacTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGaa                                                                             <  2:716485/148‑1 (MQ=255)
                                                                   acacTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGaa                                                                             >  2:2655580/1‑148 (MQ=255)
                                                                     acTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACt                                                                           <  1:2371369/148‑1 (MQ=255)
                                                                      cTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTc                                                                          <  2:216995/148‑1 (MQ=255)
                                                                                gTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGaaa                                                                <  1:128395/148‑1 (MQ=255)
                                                                                 tttCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAAt                                                               <  1:2203499/148‑1 (MQ=255)
                                                                                 tttCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAAt                                                               <  1:32049/148‑1 (MQ=255)
                                                                                  ttCGCTACAGAAAATAGTAAATAAAAAAAATAATAAGCTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATg                                                              >  2:439021/1‑148 (MQ=255)
                                                                                   tCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGt                                                             <  1:260282/148‑1 (MQ=255)
                                                                                   tCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGt                                                             <  1:750465/148‑1 (MQ=255)
                                                                                     gCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCg                                                           <  2:2125395/148‑1 (MQ=255)
                                                                                      cTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGa                                                          <  1:955632/148‑1 (MQ=255)
                                                                                      cTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGa                                                          >  1:2862843/1‑148 (MQ=255)
                                                                                      cTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGa                                                          <  1:3069875/148‑1 (MQ=255)
                                                                                         cAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGaata                                                       <  2:2520301/148‑1 (MQ=255)
                                                                                         cAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGaata                                                       >  1:2072306/1‑148 (MQ=255)
                                                                                             aaaTAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGaataataa                                                   >  1:1997609/1‑148 (MQ=255)
                                                                                              aaTAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGaataataat                                                  <  1:1539772/148‑1 (MQ=255)
                                                                                               aTAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACGAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAataataata                                                 <  2:3438685/148‑1 (MQ=255)
                                                                                                 aGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAAtata                                               >  2:1569124/1‑148 (MQ=255)
                                                                                                 aGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAAtata                                               >  2:2410636/1‑148 (MQ=255)
                                                                                                  gTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATaa                                              <  1:3432882/148‑1 (MQ=255)
                                                                                                   taaataaaAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATaaa                                             >  2:77991/1‑148 (MQ=255)
                                                                                                     aataaaAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAg                                           <  1:401270/148‑1 (MQ=255)
                                                                                                      ataaaAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGt                                          >  1:1097565/1‑148 (MQ=255)
                                                                                                       taaaAAAAATAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGtt                                         >  1:884796/1‑148 (MQ=255)
                                                                                                         aaaaaaaTAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCa                                       >  1:975629/1‑148 (MQ=255)
                                                                                                         aaaaaaaTAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCa                                       <  2:290323/148‑1 (MQ=255)
                                                                                                          aaaaaaTAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCAt                                      <  1:3197745/148‑1 (MQ=255)
                                                                                                          aaaaaaTAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCAt                                      <  2:2345735/148‑1 (MQ=255)
                                                                                                            aaaaTAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCAttt                                    <  2:1075513/148‑1 (MQ=255)
                                                                                                             aaaTAATAAACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTa                                   >  1:2677247/1‑148 (MQ=255)
                                                                                                             aaaTAATAAACTACAAATAAAATGCGTCACATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTa                                   >  1:489478/1‑148 (MQ=255)
                                                                                                                 aataaACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATg                               >  2:3230409/1‑148 (MQ=255)
                                                                                                                  ataaACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGa                              >  2:1344778/1‑148 (MQ=255)
                                                                                                                   taaACTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGaa                             <  2:828915/148‑1 (MQ=255)
                                                                                                                    aaaCTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAAc                            >  1:1313542/1‑148 (MQ=255)
                                                                                                                    aaaCTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAAc                            >  1:50933/1‑148 (MQ=255)
                                                                                                                    aaaCTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATATAAATGAAc                            >  2:1738927/1‑148 (MQ=255)
                                                                                                                      aCTACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCa                          >  1:3377092/1‑148 (MQ=255)
                                                                                                                        tACAAATAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCaga                        >  1:1490667/1‑148 (MQ=255)
                                                                                                                           aaaTAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGat                     >  1:1674860/1‑148 (MQ=255)
                                                                                                                            aaTAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGata                    <  1:1359876/148‑1 (MQ=255)
                                                                                                                            aaTAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGata                    <  1:2442081/148‑1 (MQ=255)
                                                                                                                            aaTAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGata                    >  1:3413354/1‑148 (MQ=255)
                                                                                                                             aTAAAATGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGataa                   <  2:1434844/148‑1 (MQ=255)
                                                                                                                               aaaaTGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGataata                 <  1:2238698/148‑1 (MQ=255)
                                                                                                                                aactGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAatat                <  1:1821611/145‑1 (MQ=255)
                                                                                                                                aaaTGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAatat                <  1:1064949/148‑1 (MQ=255)
                                                                                                                                 aaTGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATAtt               >  2:2825808/1‑148 (MQ=255)
                                                                                                                                 aaTGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATAtt               >  1:1461583/1‑148 (MQ=255)
                                                                                                                                  aTGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATAttt              <  2:3026390/148‑1 (MQ=255)
                                                                                                                                  aTGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATAttt              <  2:1256890/148‑1 (MQ=255)
                                                                                                                                  aTGCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATAttt              >  2:2029339/1‑148 (MQ=255)
                                                                                                                                    gCGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATAttttt            >  1:2562882/1‑148 (MQ=255)
                                                                                                                                     cGTCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTa           <  1:3550052/148‑1 (MQ=255)
                                                                                                                                       tCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACa         >  2:690014/1‑148 (MQ=255)
                                                                                                                                       tCATATTCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACCTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACa         >  1:821244/1‑148 (MQ=255)
                                                                                                                                             tCAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACATGCCGa   >  2:1823419/1‑148 (MQ=255)
                                                                                                                                              cAATTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACATGCCGAt  <  2:2384715/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ATGAAAATATTAAGAGCGATCCATCATGGCCGAATGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAGAAAATAGTAAATAAAAAAAATAATAAACTACAAATAAAATGCGTCATATTCAACTCATTGCGTGCACTAGGCTATGATAAAGAAAATTCACTTAAGCGTGTCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAAATGAACCAGAGATAATATTTTTACATGCCGAT  >  CP009273/2376814‑2377103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑