Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,404,772 C→A 100% A20A (GCG→GCT)  yfbS ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,404,7720CA95.9% 593.3 / 14.6 197A20A (GCG→GCT) yfbSputative transporter
Reads supporting (aligned to +/- strand):  ref base C (6/2);  new base A (114/75);  total (120/77)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 197 total aligned reads displayed.

TCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTG  >  CP009273/2404629‑2404915
                                                                                                                                                |                                                                                                                                               
tCGCCTAAAATAAACCTGGCGCCAATCAGGACACTTTTTGTTCCATAAACGCCGTAAATTAGTTGGGGTTGTTTAAGGGTTCCGTTTAAT‑CAAACGAAAAAAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCaa                                                                                                                                             <  2:51163/148‑1 (MQ=255)
  gCCAATAATAAACAAGGCGGCAATCAGGAAAACTTTAGTATAACAAAAGCCGTAAAATACTTCTGGTAGTGTCAGCGTTCCGCTTAATCCAACCG‑CTACAATACCAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAAc                                                                                                                                           <  2:2075328/148‑1 (MQ=255)
    cAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACaa                                                                                                                                         >  1:3479799/1‑148 (MQ=255)
    cAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACaa                                                                                                                                         >  2:2852704/1‑148 (MQ=255)
     aataatAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAg                                                                                                                                        >  1:1100685/1‑148 (MQ=255)
     aataatAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAg                                                                                                                                        >  1:3177860/1‑148 (MQ=255)
      ttaataaACAAGGCCACATACAGAACAACTTTAGTATAATAAACGCCGAAAATTATTTCTGGGACTTTCTGCGTTCCGTTTATTGAAAACG‑CCAAAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGa                                                                                                                                       <  2:2541145/147‑1 (MQ=255)
       taataaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGAc                                                                                                                                      >  2:456052/1‑148 (MQ=255)
       taataaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGAc                                                                                                                                      >  2:1223182/1‑148 (MQ=255)
          taaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGAt                                                                                                                                   >  1:512236/1‑148 (MQ=255)
              cAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACACTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCa                                                                                                                               >  2:2661835/1‑148 (MQ=255)
              cAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCa                                                                                                                               >  2:2148298/1‑148 (MQ=255)
               aaGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCAAACGCACTCTGCACGAAGCAAACAAGACTATGGCaa                                                                                                                              >  1:2466112/1‑148 (MQ=255)
                 ggcggcAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACg                                                                                                                            >  1:2144332/1‑148 (MQ=255)
                  gcggcAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACgg                                                                                                                           >  1:1561987/1‑148 (MQ=255)
                    ggaaaTCAGGACACCTTTAGTAAAAAAAACGCCGTAAAAAACTTCGGGTAGTGAAAGGTTCCCGCTTAATCCAACCG‑CTAAAATAAAAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGcc                                                                                                                         <  2:3380215/145‑1 (MQ=255)
                       aaTCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGt                                                                                                                      >  2:1531505/1‑148 (MQ=255)
                       aaTCAGGACAACGTTAGGATAATAAAAGCCGTAAAATACTTCGGGTACTGTCAGCGTTCCGCTTAATGCAACCG‑CTAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGt                                                                                                                      <  2:1390681/148‑1 (MQ=255)
                         tCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTaa                                                                                                                    >  2:1001061/1‑148 (MQ=255)
                         tCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTaa                                                                                                                    >  2:2441612/1‑148 (MQ=255)
                         tCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTaa                                                                                                                    >  2:1469191/1‑148 (MQ=255)
                          cAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAAt                                                                                                                   >  2:3344078/1‑148 (MQ=255)
                          cAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAAt                                                                                                                   >  1:1053463/1‑148 (MQ=255)
                               cAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAg                                                                                                              >  2:3530556/1‑148 (MQ=255)
                               cAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAg                                                                                                              >  2:2902981/1‑148 (MQ=255)
                               cAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAg                                                                                                              >  2:1074970/1‑148 (MQ=255)
                               cAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAg                                                                                                              >  2:2288120/1‑148 (MQ=255)
                                       gtaTCAAAAAAGCGTTAAATTACTTGTGGGACTGTCTGCTTTCCGATTAATGCAACCG‑CGAAAATAACAAACACCGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCaaa                                                                                                      <  2:833203/146‑1 (MQ=255)
                                       ggATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCaaa                                                                                                      >  2:842440/1‑148 (MQ=255)
                                       ggATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCaaa                                                                                                      >  1:1281675/1‑148 (MQ=255)
                                        taTCATAAAAGCCTAAAATTACTTGTGGCTTTGTCAGTGTTCCTTTTATTTAAACCG‑CCAAAAAAAAAAACACCGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAAt                                                                                                     <  1:2809578/147‑1 (MQ=255)
                                         aTCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATc                                                                                                    >  2:3444359/1‑148 (MQ=255)
                                           cAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCaa                                                                                                  >  1:2170764/1‑148 (MQ=255)
                                           aataacaGCCGCAAAATATTTTGGGTAGTGTCGGGTTTCCTCTTAATCAAAACG‑CTAAAAAAAAAAACACAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCaa                                                                                                  <  2:985417/142‑1 (MQ=255)
                                           aataaaaGCGGTAAAATACTTCTGGGACTGTCTGCGTTCCGCTTAATGAAAACG‑CCAAAATAAAAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCaa                                                                                                  <  2:2606634/145‑1 (MQ=255)
                                             gAAAAGCCGTAAAATACTTCTGGCAGTGTAAGGTTTCCTATTATTGCAACCG‑GCAAAATAACAAACACAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAAtt                                                                                                <  2:72487/148‑1 (MQ=255)
                                             caaaaGCCTTAAAATACTTGTGGTAGTGTATGCGTACGGTATAATCAAAACG‑CAAAAAAAAAAAACCACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAAtt                                                                                                <  1:1915268/147‑1 (MQ=255)
                                               aaaGCCGTAAAATACTTCTGGGACTTTCTGCGTTCCGATTAATCCAAACG‑CTAAAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCa                                                                                              <  2:2305420/148‑1 (MQ=255)
                                               aaaGCCGCAAAATACTTCTGGGACTGTCAGCGTTCCGATTAATGCAAACG‑CGAAAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCa                                                                                              <  2:1886563/148‑1 (MQ=255)
                                                aaGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCAc                                                                                             >  2:1940323/1‑148 (MQ=255)
                                                aaGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCAc                                                                                             >  1:2933925/1‑148 (MQ=255)
                                                   ccGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGt                                                                                          >  1:681816/1‑148 (MQ=255)
                                                   ccGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGt                                                                                          >  1:1885042/1‑148 (MQ=255)
                                                     ttaaaaTACTTGTGGTACTTTCTGCTTTCCTCTTAATCAAAACG‑CAAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTc                                                                                        <  1:1794877/146‑1 (MQ=255)
                                                       aaaaTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTcac                                                                                      >  1:300828/1‑148 (MQ=255)
                                                         attattgcTGGGACTGTCAGCGTTCCTATTAATGAAAACG‑CGAAAATAAAAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACaa                                                                                    <  2:3076195/141‑1 (MQ=255)
                                                         aaTACTTCTGGTACTTTCAGCGTTCCGCTTAATGCAACCG‑CGACAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACaa                                                                                    <  2:2370664/148‑1 (MQ=255)
                                                         aaTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACaa                                                                                    >  1:1760020/1‑148 (MQ=255)
                                                         aaTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACaa                                                                                    >  1:2070408/1‑148 (MQ=255)
                                                         aaTACTTCTGGGACTGTCAGCGTTCCGCTTAATGAAACCG‑CGAAAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACaa                                                                                    <  2:2370489/148‑1 (MQ=255)
                                                           tACTTTTGGTACTGTAAGCGTTCCTCTTAATCCAAACG‑CGAAAAAAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACt                                                                                  <  2:3143573/148‑1 (MQ=255)
                                                           tACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACt                                                                                  >  1:555533/1‑148 (MQ=255)
                                                             cTTCTGGGACTGTATGCGTTCCGATTAATGAAAACG‑CTAAAATAAAAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTa                                                                                <  2:2778470/148‑1 (MQ=255)
                                                              ttgtGGTACTGTCAGCGTTCCTCTAAATCCAACCG‑ATAAAAAAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTAt                                                                               <  1:2251560/145‑1 (MQ=255)
                                                              ttgtGGGTCTTTCTGGGTTCCGCTTAATGCAACCG‑CCAAAAAAAAAAACACCGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTAt                                                                               <  1:888630/145‑1 (MQ=255)
                                                              ttCTGGGACTGTCAGCTTTCCGATTAATGAAACCG‑CTAAAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTAt                                                                               <  2:556101/148‑1 (MQ=255)
                                                                   ggACTGTCAGGTTTCCTTTTAATTAAACCG‑GTACAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTg                                                                          <  2:464161/148‑1 (MQ=255)
                                                                   ggACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTg                                                                          >  1:2205407/1‑148 (MQ=255)
                                                                     aCTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGtt                                                                        >  2:1543310/1‑148 (MQ=255)
                                                                      gtGTCAGCGTTCCGTTTAATGCAACCG‑CTAAAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTa                                                                       <  2:3067828/147‑1 (MQ=255)
                                                                        gTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAAt                                                                     >  2:1055151/1‑148 (MQ=255)
                                                                         tCAGCGTTCCGCTTAATGCACACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAAtt                                                                    >  1:1940655/1‑148 (MQ=255)
                                                                          cAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTg                                                                   >  1:3136336/1‑148 (MQ=255)
                                                                          cAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTg                                                                   >  1:1932892/1‑148 (MQ=255)
                                                                           aGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGa                                                                  >  2:1685626/1‑148 (MQ=255)
                                                                                tccttttAATCAAAAAG‑AGAAAATAAAAAAAACAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAggggg                                                             <  1:2361341/143‑1 (MQ=255)
                                                                                tCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAggggg                                                             >  1:1901017/1‑148 (MQ=255)
                                                                                tCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAggggg                                                             >  2:1895948/1‑148 (MQ=255)
                                                                                 ccGCTTAATGCAAACG‑CGAAAAAAACAAAAAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGa                                                            <  2:3177860/148‑1 (MQ=255)
                                                                                    cTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGa                                                         >  1:2920986/1‑148 (MQ=255)
                                                                                    attAATGCAACCG‑CCAAAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGa                                                         <  1:3247950/147‑1 (MQ=255)
                                                                                         tGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGa                                                    >  1:3179650/1‑148 (MQ=255)
                                                                                          gCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCACCGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTAATCCTTGTTAATTGAGGGGGATGACTTGAt                                                   >  2:355218/1‑148 (MQ=255)
                                                                                          gCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGAt                                                   >  2:356735/1‑148 (MQ=255)
                                                                                           cAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGAtt                                                  >  2:3287919/1‑148 (MQ=255)
                                                                                                cgctaaaaTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGc                                              <  2:1846804/142‑1 (MQ=255)
                                                                                                  cgACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCa                                            >  1:3306082/1‑148 (MQ=255)
                                                                                                    aCAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATa                                          >  1:2188027/1‑148 (MQ=255)
                                                                                                     cAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaa                                         >  1:2517755/1‑148 (MQ=255)
                                                                                                     cAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaa                                         >  1:2570143/1‑148 (MQ=255)
                                                                                                       aTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaaaa                                       >  1:2512239/1‑148 (MQ=255)
                                                                                                            aacccccgCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGcc                                  <  1:2429650/141‑1 (MQ=255)
                                                                                                             aacaacGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGccc                                 <  1:2661829/148‑1 (MQ=255)
                                                                                                                  aGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcgacg                            <  2:2282021/148‑1 (MQ=255)
                                                                                                                    cGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACgag                          <  2:1799917/148‑1 (MQ=255)
                                                                                                                    cGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACgag                          <  2:53269/148‑1 (MQ=255)
                                                                                                                       ccGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACg                       >  1:1666631/1‑148 (MQ=255)
                                                                                                                         gCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCAAAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACggg                     <  1:3356961/148‑1 (MQ=255)
                                                                                                                             ccATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTa                 >  2:570058/1‑148 (MQ=255)
                                                                                                                               aTACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTcaa               >  2:3139798/1‑148 (MQ=255)
                                                                                                                               aTACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTaaa               >  1:80539/1‑148 (MQ=255)
                                                                                                                                  cGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCa            <  1:258643/148‑1 (MQ=255)
                                                                                                                                    cACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCTATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCAATAAAAAAGCCCCGACGAGACGGGGCTAAATCATg          <  2:877100/148‑1 (MQ=255)
                                                                                                                                    cACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATg          <  2:1264184/148‑1 (MQ=255)
                                                                                                                                    cACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATg          <  2:876760/148‑1 (MQ=255)
                                                                                                                                     aCTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGa         <  2:1100685/148‑1 (MQ=255)
                                                                                                                                      ctctGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGAt        >  1:2666553/1‑148 (MQ=255)
                                                                                                                                       tctGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATc       <  2:928505/148‑1 (MQ=255)
                                                                                                                                       tctGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATc       <  2:3479799/148‑1 (MQ=255)
                                                                                                                                       tctGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAAACAATTCACCGTTCACAACTTATCATTGTTAATTGTGGGGTATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATc       <  1:1531505/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
TCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACG‑CGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTG  >  CP009273/2404629‑2404915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑