Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,424,074 C→T 100% D57N (GAC→AAC)  cvpA ← colicin V production membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,424,0740CT100.0% 647.9 / ‑6.7 195D57N (GAC→AAC) cvpAcolicin V production membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (96/98);  minor base G (1/0);  total (97/98)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 199 total aligned reads displayed.

CCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGAT  >  CP009273/2423929‑2424218
                                                                                                                                                 |                                                                                                                                                
ccGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGtttt                                                                                                                                                >  1:47789/1‑148 (MQ=255)
ccGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGtttt                                                                                                                                                >  2:3175105/1‑148 (MQ=255)
ccGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGtttt                                                                                                                                                >  1:1915491/1‑148 (MQ=255)
 cGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTc                                                                                                                                               >  2:3388737/1‑148 (MQ=255)
   aaaCAGACGCCCCGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCTAACCAGTTCGTTTTCaa                                                                                                                                             <  1:2683668/148‑1 (MQ=255)
   aaaCAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCaa                                                                                                                                             <  2:1284861/148‑1 (MQ=255)
   aaaCAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCaa                                                                                                                                             >  2:208778/1‑148 (MQ=255)
     aCAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCTATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAg                                                                                                                                           <  2:365330/148‑1 (MQ=255)
       aGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGcc                                                                                                                                         >  1:2695485/1‑148 (MQ=255)
         aCGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCg                                                                                                                                       >  2:2747760/1‑148 (MQ=255)
         aCGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCg                                                                                                                                       <  2:1577259/148‑1 (MQ=255)
         aCGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTGTTCAAAGCCCg                                                                                                                                       >  2:155987/1‑148 (MQ=255)
            cccAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTaa                                                                                                                                    <  2:1774564/148‑1 (MQ=255)
             ccAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTaaa                                                                                                                                   >  2:470656/1‑148 (MQ=255)
                gCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCa                                                                                                                                <  2:1704174/148‑1 (MQ=255)
                gCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCa                                                                                                                                <  2:79768/148‑1 (MQ=255)
                 cACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAg                                                                                                                               >  1:2757488/1‑148 (MQ=255)
                 cACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAg                                                                                                                               <  1:1881605/148‑1 (MQ=255)
                 cACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAg                                                                                                                               <  1:2573266/148‑1 (MQ=255)
                     cGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTg                                                                                                                           <  1:1665258/148‑1 (MQ=255)
                      gATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGa                                                                                                                          <  2:2695368/148‑1 (MQ=255)
                        tCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACa                                                                                                                        <  2:3310582/148‑1 (MQ=255)
                           gTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGt                                                                                                                     >  2:1400389/1‑148 (MQ=255)
                           gTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGt                                                                                                                     >  2:1399626/1‑148 (MQ=255)
                             gCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTaa                                                                                                                   <  2:348860/148‑1 (MQ=255)
                              ccTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAg                                                                                                                  <  1:2459432/148‑1 (MQ=255)
                              ccTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAg                                                                                                                  <  2:2221607/148‑1 (MQ=255)
                                tGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAgtg                                                                                                                >  2:2010278/1‑148 (MQ=255)
                                  aCAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTgta                                                                                                              <  2:596898/148‑1 (MQ=255)
                                     aCCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTgtagta                                                                                                           >  1:1261887/1‑148 (MQ=255)
                                        ccGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATg                                                                                                        >  1:1522566/1‑148 (MQ=255)
                                               ctcCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGca                                                                                                 <  2:3159233/148‑1 (MQ=255)
                                               ctcCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGca                                                                                                 <  1:1946380/148‑1 (MQ=255)
                                                   accaACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGcaacaa                                                                                             >  2:214512/1‑148 (MQ=255)
                                                   accaACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGcaacaa                                                                                             <  1:1753274/148‑1 (MQ=255)
                                                     caACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACaaag                                                                                           <  1:2051862/148‑1 (MQ=255)
                                                       aCTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACaaagaa                                                                                         >  1:3206057/1‑148 (MQ=255)
                                                          ggCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGc                                                                                      <  2:2628440/148‑1 (MQ=255)
                                                          ggCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGc                                                                                      <  1:3554394/148‑1 (MQ=255)
                                                            ccTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGcac                                                                                    >  1:335363/1‑148 (MQ=255)
                                                             cTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGcaca                                                                                   >  1:1617547/1‑148 (MQ=255)
                                                              tATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACaa                                                                                  >  1:3025906/1‑148 (MQ=255)
                                                                tCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTACGTGTAGTAATGACTGGCAACAAAGAAAGCACAAcc                                                                                >  2:2928061/1‑148 (MQ=255)
                                                                tCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAAcc                                                                                >  2:2847246/1‑148 (MQ=255)
                                                                 cACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAAccc                                                                               <  2:1726905/148‑1 (MQ=255)
                                                                  aCGAAGTTCACTATAGCACCAACGATCATGGTAGCGATAAACAGTACCGCGATGTCAATCCCATTTAGAACCAGTTCGTTTTCAAAGCCCGTAATCCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAAcccc                                                                              <  2:1412883/148‑1 (MQ=255)
                                                                   cGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCa                                                                             >  1:3455631/1‑148 (MQ=255)
                                                                    gAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCAt                                                                            >  2:2370828/1‑148 (MQ=255)
                                                                     aaGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATg                                                                           <  1:1018105/148‑1 (MQ=255)
                                                                      aGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGt                                                                          >  2:214110/1‑148 (MQ=255)
                                                                      aGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGt                                                                          >  2:2475238/1‑148 (MQ=255)
                                                                      aGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGt                                                                          >  1:929488/1‑148 (MQ=255)
                                                                        ttCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCa                                                                        <  2:432261/148‑1 (MQ=255)
                                                                        ttCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCa                                                                        <  1:2559669/148‑1 (MQ=255)
                                                                        ttCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCa                                                                        >  2:184104/1‑148 (MQ=255)
                                                                         tCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCAc                                                                       >  2:2107967/1‑148 (MQ=255)
                                                                            cGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAg                                                                    >  2:57976/1‑148 (MQ=255)
                                                                            cGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAg                                                                    <  1:2658618/148‑1 (MQ=255)
                                                                              aTAGCACCAACGATCAGTGTAGAGATAAACAGTACCGCGATTTCAATCACATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCg                                                                  <  2:1522566/148‑1 (MQ=255)
                                                                               tAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGa                                                                 >  2:3088851/1‑148 (MQ=255)
                                                                                aGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGAt                                                                <  2:849506/148‑1 (MQ=255)
                                                                                      aaCGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCt                                                          <  2:2251068/148‑1 (MQ=255)
                                                                                       aCGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCtt                                                         <  1:759614/148‑1 (MQ=255)
                                                                                        cGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTc                                                        <  2:2482964/148‑1 (MQ=255)
                                                                                         gATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCa                                                       <  1:1048008/148‑1 (MQ=255)
                                                                                          aTCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCAc                                                      >  2:744479/1‑148 (MQ=255)
                                                                                              gggTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAAc                                                  >  1:3276248/1‑148 (MQ=255)
                                                                                                  aGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCCTGTCACCAGCGATAACGCTTCACGAACAAAg                                              <  2:3199485/148‑1 (MQ=255)
                                                                                                   gCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGc                                             <  1:3481456/148‑1 (MQ=255)
                                                                                                       tAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCcgcg                                         >  1:2970524/1‑148 (MQ=255)
                                                                                                        aaaCAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCgg                                        <  1:2977781/148‑1 (MQ=255)
                                                                                                        aaaCAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCgg                                        <  1:3388671/148‑1 (MQ=255)
                                                                                                          aCAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGAt                                      >  2:432542/1‑148 (MQ=255)
                                                                                                          aCAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGAt                                      <  2:1817793/148‑1 (MQ=255)
                                                                                                          aCAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGAt                                      <  2:1817697/148‑1 (MQ=255)
                                                                                                          aCAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGAt                                      >  1:1238451/1‑148 (MQ=255)
                                                                                                              tACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAgg                                  >  1:2783014/1‑148 (MQ=255)
                                                                                                                ccGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCt                                <  1:1919145/148‑1 (MQ=255)
                                                                                                                 cgcgATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTa                               <  1:242991/148‑1 (MQ=255)
                                                                                                                     aTGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCa                           >  1:2357939/1‑148 (MQ=255)
                                                                                                                      tGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCag                          >  1:94352/1‑148 (MQ=255)
                                                                                                                      tGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCag                          >  1:2225089/1‑148 (MQ=255)
                                                                                                                      tGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCag                          >  1:2168788/1‑148 (MQ=255)
                                                                                                                          aaTCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCagagag                      >  1:279028/1‑148 (MQ=255)
                                                                                                                           aTCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAgg                     <  2:945893/148‑1 (MQ=255)
                                                                                                                            tCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGa                    <  2:551561/148‑1 (MQ=255)
                                                                                                                             cccATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGaa                   <  2:1907977/148‑1 (MQ=255)
                                                                                                                             cccATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGaa                   <  2:109011/148‑1 (MQ=255)
                                                                                                                             cccATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGaa                   >  2:664634/1‑148 (MQ=255)
                                                                                                                               cATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAATAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGaaaa                 >  1:1021465/1‑148 (MQ=255)
                                                                                                                                 tttCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAg               >  1:3228758/1‑148 (MQ=255)
                                                                                                                                   tCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCa             <  2:1474716/148‑1 (MQ=255)
                                                                                                                                     gAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAAt           <  1:562687/148‑1 (MQ=255)
                                                                                                                                      aaCCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATc          >  2:207019/1‑148 (MQ=255)
                                                                                                                                      aaCCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATc          <  2:726797/148‑1 (MQ=255)
                                                                                                                                       aCCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCa         <  1:108343/148‑1 (MQ=255)
                                                                                                                                        ccAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCAc        >  2:2117032/1‑148 (MQ=255)
                                                                                                                                        ccAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCAc        >  2:1502802/1‑148 (MQ=255)
                                                                                                                                             tCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGa   <  2:2561588/148‑1 (MQ=255)
                                                                                                                                              cGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGAt  >  1:1629818/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGAT  >  CP009273/2423929‑2424218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑