Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,438,529 A→C 10.1% F171V (TTC→GTC)  yfcA ← UPF0721 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,438,5290AC10.1% 419.0 / 3.0 190F171V (TTC→GTC) yfcAUPF0721 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (114/47);  new base C (2/24);  total (116/74)
Fisher's exact test for biased strand distribution p-value = 7.80e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 199 total aligned reads displayed.

ACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAG  >  CP009273/2438382‑2438676
                                                                                                                                                   |                                                                                                                                                   
aCTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGaa                                                                                                                                                     <  2:476157/148‑1 (MQ=255)
 cTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAg                                                                                                                                                    >  1:1753391/1‑148 (MQ=255)
    ccccACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGcc                                                                                                                                                 >  2:638389/1‑148 (MQ=255)
       caccaGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAgc                                                                                                                                              >  2:3526934/1‑148 (MQ=255)
          caGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGcgcg                                                                                                                                           >  1:554776/1‑148 (MQ=255)
          caGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGcgcg                                                                                                                                           >  1:554897/1‑148 (MQ=255)
             cATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTaa                                                                                                                                        <  2:2368017/148‑1 (MQ=255)
              aTGAGAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTaaa                                                                                                                                       <  2:280256/148‑1 (MQ=255)
              aTGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTaaa                                                                                                                                       <  1:157617/148‑1 (MQ=255)
              aTGACAAAGCCCGTCGCCCAAATCACTGTGCCGCCGAGAATAAACCGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTaaa                                                                                                                                       <  2:41096/148‑1 (MQ=255)
               tGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTaaaa                                                                                                                                      >  2:1117939/1‑148 (MQ=255)
                  cAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTTAGTATTTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCTTAAAACGa                                                                                                                                   <  1:859741/148‑1 (MQ=255)
                  cAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGa                                                                                                                                   >  1:1598152/1‑148 (MQ=255)
                   aacgCCGGTCGCCCAAATCACTTTGCCGCCGATAATAAACAGCATTAAACCGCCTATGTTTTACGTTGCTTTGTGTAATTTGGCGTGAGCCGTGGCTTTGGCGTGTTTAAATCCGAACAGCGTAACGAAGGCCAGCGCGTAAAACGAc                                                                                                                                  <  2:1635607/145‑1 (MQ=255)
                   aaaGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGTGGTTAAATCCGCACAGCTTAACGAAGGCCAGCGCGTAAAACGAc                                                                                                                                  <  2:3023609/148‑1 (MQ=255)
                     aGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGAccc                                                                                                                                >  2:2140973/1‑148 (MQ=255)
                      gCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCg                                                                                                                               >  2:722538/1‑148 (MQ=255)
                       cccGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCgg                                                                                                                              >  2:2472106/1‑148 (MQ=255)
                                cAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGc                                                                                                                     >  2:1241733/1‑148 (MQ=255)
                                  aaTCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCa                                                                                                                   >  1:1938756/1‑148 (MQ=255)
                                   aTCACTTTGCCGCCGCGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCaa                                                                                                                  <  2:1057086/148‑1 (MQ=255)
                                    tCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCaaa                                                                                                                 >  1:2574374/1‑148 (MQ=255)
                                       cTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGaa                                                                                                              >  2:168094/1‑148 (MQ=255)
                                       cTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGaa                                                                                                              >  2:116873/1‑148 (MQ=255)
                                        tttGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAAt                                                                                                             >  1:1662296/1‑148 (MQ=255)
                                              gccgATAATAAACAGCAGTAAACCGCCGATTTTTGACGTGGCGTTTTGTAATTTGGGTTGAGCCGTGGGTTTGGCGAGTTTAAATCCGCACAGCGTAACGAAGGCCTGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCcatca                                                                                                       <  2:1317748/148‑1 (MQ=255)
                                              gccgACAATAAACATAAGTAAACCGCCTATGTTTTACGTTGCGTTGAGTAATTTGGAGTTAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGATGGCCTGCGCTTAACACGACCCGGCTGCCGGGCCAATGAATCcatca                                                                                                       <  2:1496528/148‑1 (MQ=255)
                                                 gagaATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATaa                                                                                                    >  2:1236629/1‑148 (MQ=255)
                                                   taaTAAACAGCAATAACCCGCCTTTTTTTTAGTTTGGTTTTTTTATTTTGGGGTGTGCGTTGGTTTTGGGTTGTTAAAATCCGAAAAGCTTAACTATGGCCAGCGCTAAACACGACCCGGCAGCCGGGCCAAAGAATCCATCATaaaa                                                                                                  <  1:51051/147‑1 (MQ=255)
                                                   taaTAAACAGATTTAACCCGCCTTTGTTTGTTGTTGGTTTGTTTATTTTGGGGTGTGCGGTGGTTTTGGCTTTTTTAAATCCGCAATGCTTAACTACGGCCTGCGCTAAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATaaaa                                                                                                  <  1:717575/147‑1 (MQ=255)
                                                   gaATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATaaaa                                                                                                  >  2:2240810/1‑148 (MQ=255)
                                                   gaATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATaaaa                                                                                                  >  2:1336032/1‑148 (MQ=255)
                                                    aaTAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATaaaaa                                                                                                 >  2:95508/1‑148 (MQ=255)
                                                     aTAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAAc                                                                                                >  1:2420926/1‑148 (MQ=255)
                                                      tAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAAcc                                                                                               >  2:712308/1‑148 (MQ=255)
                                                       aaaCCGCAGTAAACGGCCGTTGTTTGAGGTTGCGTTGAGTAATTTGGCGTGAGGCGTGGCTTTGGCGAGGTTAAATCCGAAATGGGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCg                                                                                              <  2:3460134/148‑1 (MQ=255)
                                                       aaaCAGCATTAACCCGCCGTTGTTTGAGGTTGGGTTTTTTATTTGGGCGTGAGCGGTGGTTTTGGCGAGGTTAAATCCGCACAGCTTAACGACGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCg                                                                                              <  1:528624/148‑1 (MQ=255)
                                                       aaaCAGCAGTAACCCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTGGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCg                                                                                              <  2:1708481/148‑1 (MQ=255)
                                                            gcagTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCa                                                                                         >  1:1292908/1‑148 (MQ=255)
                                                             cagTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAg                                                                                        >  2:305274/1‑148 (MQ=255)
                                                                tAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCa                                                                                     >  2:972499/1‑148 (MQ=255)
                                                                tAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCa                                                                                     >  1:1040561/1‑148 (MQ=255)
                                                                tAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCa                                                                                     >  1:1041595/1‑148 (MQ=255)
                                                                 aaaCCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCAc                                                                                    >  1:3332905/1‑148 (MQ=255)
                                                                    cccccgATGTTTGCCGTGGCGTTGAGTAATTGGGCGTGAGCCGTGGGTTTGGCGCGGTTAAATCCGCACAGCGTACCGAAGGCCAGCGCGAAACACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCgg                                                                                 <  2:526018/145‑1 (MQ=255)
                                                                       cctaTGTTTGACTTTGGGTTTATTAATTTGGCTTGCGCGGTGGATTTGGCTATTTTAAATCCGCACCGCTTAACTAAGGCCAGCCCTTAACACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTa                                                                              <  2:1205461/145‑1 (MQ=255)
                                                                       ccgTTGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCGTTGGCTTGGGCGAGGTTAAATCCGCACCGCGTAACGAAGCCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTa                                                                              <  2:2408024/148‑1 (MQ=255)
                                                                        cgttGTTTGACGTTGCGTTGAGTAATTGGGCGTGCGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGACGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACAGACGCAGCCACCGGCTAt                                                                             <  1:3527606/145‑1 (MQ=255)
                                                                         gATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTAtt                                                                            >  2:719074/1‑148 (MQ=255)
                                                                          aTGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTa                                                                           >  2:1233770/1‑148 (MQ=255)
                                                                          aTGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTa                                                                           >  2:1917453/1‑148 (MQ=255)
                                                                            gTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAAc                                                                         >  2:3178092/1‑148 (MQ=255)
                                                                             tttGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAATGAATCCATCATAAAAACCGACGCAGCCACCGGCTAGGAAcg                                                                        >  1:2322953/1‑148 (MQ=255)
                                                                             tttGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAAcg                                                                        >  1:2072760/1‑148 (MQ=255)
                                                                             tttGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAAcg                                                                        >  1:3054324/1‑148 (MQ=255)
                                                                                  cgttgcgttgAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAg                                                                   <  2:698576/148‑1 (MQ=255)
                                                                                    ttgcgttgAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAggg                                                                 >  2:3521604/1‑148 (MQ=255)
                                                                                     tgcgttgAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGt                                                                >  1:1997085/1‑148 (MQ=255)
                                                                                         ttgAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAAc                                                            >  1:3112694/1‑148 (MQ=255)
                                                                                          tgtttAATTTGGCGTGTGCGGTGGCTTTGGCGAGGTTAAATCCGCACAGCGAAACGACGGCCCGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAAcc                                                           <  2:3276889/144‑1 (MQ=255)
                                                                                          tgAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAAcc                                                           >  2:1517909/1‑148 (MQ=255)
                                                                                           gAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCg                                                          >  2:1798146/1‑148 (MQ=255)
                                                                                              tattttGGCGTGAGCCGTGGGTTTGGCGAGGTTAAATCCGCACAGCGTACCGACGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTAc                                                       <  2:50711/145‑1 (MQ=255)
                                                                                              tAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACCGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTAc                                                       <  2:3230383/148‑1 (MQ=255)
                                                                                              tAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTAc                                                       >  1:2824694/1‑148 (MQ=255)
                                                                                                ttttGGCGTGCGCCGTGGCTTGGGCGCGTTTAACTCCGCCCCGCGTACCCACGCCCCGCGCTAAACACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACAt                                                     <  1:1713769/147‑1 (MQ=255)
                                                                                                aTTGGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAACACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACAt                                                     <  2:2620230/148‑1 (MQ=255)
                                                                                                 tttGGCGTGCGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATc                                                    <  2:808060/148‑1 (MQ=255)
                                                                                                 tttGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATc                                                    >  2:1125443/1‑148 (MQ=255)
                                                                                                     gCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGgc                                                >  1:1676588/1‑148 (MQ=255)
                                                                                                       gTGAGCGGTGGTTTTGGCTTGTTTAAACCCGCACAGCGTAACGACGGCCAGCGCTTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGgcgc                                              <  1:1400680/148‑1 (MQ=255)
                                                                                                        tGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCt                                             >  2:1069932/1‑148 (MQ=255)
                                                                                                          aGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTgg                                           >  1:2508887/1‑148 (MQ=255)
                                                                                                           gCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGc                                          >  1:2873173/1‑148 (MQ=255)
                                                                                                              gTGGCTTTGGCGTGTTTAACACCCCACCGCGTAACGATGGCCAGCGCGTAACACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGt                                       <  1:829447/148‑1 (MQ=255)
                                                                                                              gTGGCTTTGGCGAGTTTAAACCCGAACAGCTAAACGAAGCCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGt                                       <  1:2235323/148‑1 (MQ=255)
                                                                                                               tGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATCAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGtc                                      >  2:53084/1‑148 (MQ=255)
                                                                                                               tGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGtc                                      >  2:53144/1‑148 (MQ=255)
                                                                                                               gggttttGGGGTGGATAAAGCCCCACTGCTAAACGTCGCCCGGCGCTAACAACGCCCCGGCAGCCGGGCCAAAGAATCCTTCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGtc                                      <  1:2687628/144‑1 (MQ=255)
                                                                                                                ggCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGtct                                     >  1:723273/1‑148 (MQ=255)
                                                                                                                ggCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGtct                                     >  2:2189194/1‑148 (MQ=255)
                                                                                                                  cTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGtcttc                                   >  1:1985187/1‑148 (MQ=255)
                                                                                                                  cTTGGGCGCGGTTAAATCCGCACCGCGTAACGACGCCCCGGGCGTAAAACGACCCGGCCGCCGGGCCAAAGAATCCATCATACAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGtcttc                                   <  1:1117762/148‑1 (MQ=255)
                                                                                                                    ttGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTcttctt                                 >  2:1537087/1‑148 (MQ=255)
                                                                                                                          aGGTTAAATCCGCACAGCGTAACGAAGGCCCGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTa                           >  2:3115541/1‑148 (MQ=255)
                                                                                                                              tAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCtt                       <  2:2529894/148‑1 (MQ=255)
                                                                                                                                 aTCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTgg                    >  2:3315509/1‑148 (MQ=255)
                                                                                                                                   ccGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCa                  >  2:1967519/1‑148 (MQ=255)
                                                                                                                                    cGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCAt                 <  2:2948121/148‑1 (MQ=255)
                                                                                                                                      cacaGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCa               >  1:613270/1‑148 (MQ=255)
                                                                                                                                         aGCTTAACGACGGCCAGCGCTTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCa            <  2:2389487/148‑1 (MQ=255)
                                                                                                                                         aGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTCTTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCa            >  2:2720587/1‑148 (MQ=255)
                                                                                                                                           cGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAg          >  1:3452517/1‑148 (MQ=255)
                                                                                                                                           cGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAg          <  1:1790532/148‑1 (MQ=255)
                                                                                                                                              aaCGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGaaa       >  2:1275298/1‑148 (MQ=255)
                                                                                                                                              aaCGAAGGCCAGCGCGAAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGaaa       <  1:2825127/148‑1 (MQ=255)
                                                                                                                                               acgacgGCCAGCGCGTAACACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAAt      <  2:3291345/148‑1 (MQ=255)
                                                                                                                                                  aaGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATaga   <  1:1125432/148‑1 (MQ=255)
                                                                                                                                                  aaGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATaga   <  2:1753391/148‑1 (MQ=255)
                                                                                                                                                   aGGCCAGCGCGTGAAACGACCCGGCAGCAGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCCGCAATTAACGCGAAGGGTACACCGTACCTCCGGCGCTGGCGGACTTCTTCACCTAGCTTTCGCAACAGACAGAAATagag  >  2:1938042/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGGGCCAAAGAATCCATCATAAAAACCGACGCAGCCACCGGCTATTAACGCGAAGGGTAAACCGTACATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAG  >  CP009273/2438382‑2438676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑