Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,498,569 T→A 5.1% Q153L (CAG→CTG)  ypdE ← aminopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,498,5690TA 5.1% 552.9 / 15.5 176Q153L (CAG→CTG) ypdEaminopeptidase
Reads supporting (aligned to +/- strand):  ref base T (90/77);  new base A (7/2);  total (97/79)
Fisher's exact test for biased strand distribution p-value = 1.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

Only 100 of 179 total aligned reads displayed.

AATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGCCGGTCGCCGTCAAGCAGGCCTGGAATTTTGCACT  >  CP009273/2498422‑2498716
                                                                                                                                                   |                                                                                                                                                   
aaTCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCGAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCt                                                                                                                                                     >  1:1968384/1‑148 (MQ=255)
  tCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGa                                                                                                                                                   >  2:455287/1‑148 (MQ=255)
   cccACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGaa                                                                                                                                                  >  1:2037995/1‑148 (MQ=255)
     cACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGaaaa                                                                                                                                                <  1:3308680/148‑1 (MQ=255)
      aCCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAg                                                                                                                                               <  2:2332778/148‑1 (MQ=255)
       ccTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGt                                                                                                                                              <  2:3457875/148‑1 (MQ=255)
        ctctTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTg                                                                                                                                             >  1:2380867/1‑148 (MQ=255)
               cTGGAACTAGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCaa                                                                                                                                      <  2:3228135/148‑1 (MQ=255)
                    aCTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTg                                                                                                                                 <  1:1176080/148‑1 (MQ=255)
                     cTTGCCACCAGCCACACTTCCGCAGGAAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGACACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGa                                                                                                                                >  2:2882668/1‑148 (MQ=255)
                       tGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGAcg                                                                                                                              <  2:1528731/148‑1 (MQ=255)
                             caGCCACACTTCCGCAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCg                                                                                                                        >  1:2536959/1‑148 (MQ=255)
                              aGCCACACTTCCGCAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGc                                                                                                                       <  2:2376240/148‑1 (MQ=255)
                              aGCCACACTTCCGCAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGc                                                                                                                       >  1:1556582/1‑148 (MQ=255)
                              aGCCACACTTCCGCAGGTAGTTCGGCGTCGTGCAGGTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGc                                                                                                                       <  2:1867915/148‑1 (MQ=255)
                                ccACACTTCCGCAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCg                                                                                                                     >  2:1227152/1‑148 (MQ=255)
                                 cacaCTTCCGCAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCgg                                                                                                                    >  1:583339/1‑148 (MQ=255)
                                 cacaCTTCCGCAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCAGAAAAGTGGTATCAAACGTGACGCGATCGCCgg                                                                                                                    >  2:2132473/1‑148 (MQ=255)
                                   cacTTCCGCAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGa                                                                                                                  <  1:1681496/148‑1 (MQ=255)
                                   cacTTCCGCAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGa                                                                                                                  <  1:1681484/148‑1 (MQ=255)
                                      ttCCGCAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGa                                                                                                               >  1:1758393/1‑148 (MQ=255)
                                           cAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTcc                                                                                                          >  2:3082443/1‑148 (MQ=255)
                                           cAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTcc                                                                                                          <  1:3455806/148‑1 (MQ=255)
                                           cAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTcc                                                                                                          <  1:3455661/148‑1 (MQ=255)
                                             ggTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCg                                                                                                        >  1:2939110/1‑148 (MQ=255)
                                              gTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGc                                                                                                       <  1:86/148‑1 (MQ=255)
                                              gTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGc                                                                                                       >  1:2773925/1‑148 (MQ=255)
                                              gTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGc                                                                                                       <  1:71/148‑1 (MQ=255)
                                               tAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGcc                                                                                                      >  2:1363501/1‑148 (MQ=255)
                                                  ttCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGTCGAATTCCCGCCTGc                                                                                                   <  2:3323285/148‑1 (MQ=255)
                                                  ttCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGc                                                                                                   >  2:272876/1‑148 (MQ=255)
                                                    cGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCAt                                                                                                 >  2:811453/1‑148 (MQ=255)
                                                      gCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCa                                                                                               <  2:1083630/148‑1 (MQ=255)
                                                         tcgtGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACtt                                                                                            <  2:1020553/148‑1 (MQ=255)
                                                         tcgtGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACtt                                                                                            <  2:1247870/148‑1 (MQ=255)
                                                             gCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTc                                                                                        >  1:2604569/1‑148 (MQ=255)
                                                                 ttCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAg                                                                                    >  1:3010442/1‑148 (MQ=255)
                                                                  tCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAgg                                                                                   <  2:859147/148‑1 (MQ=255)
                                                                  tCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAgg                                                                                   <  1:1928674/148‑1 (MQ=255)
                                                                  tCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAgg                                                                                   <  1:131842/148‑1 (MQ=255)
                                                                      gcAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAgcg                                                                               <  1:1481782/148‑1 (MQ=255)
                                                                         gTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAgcgcgc                                                                            >  1:2552629/1‑148 (MQ=255)
                                                                          tAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCa                                                                           >  1:1919276/1‑148 (MQ=255)
                                                                          tAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCa                                                                           >  2:2248553/1‑148 (MQ=255)
                                                                          tAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCa                                                                           >  2:3548132/1‑148 (MQ=255)
                                                                          tAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCa                                                                           >  2:2693913/1‑148 (MQ=255)
                                                                           aaCGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCAc                                                                          >  1:1048065/1‑148 (MQ=255)
                                                                            aCGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCAcc                                                                         >  2:169321/1‑148 (MQ=255)
                                                                            aCGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCAcc                                                                         >  2:3544590/1‑148 (MQ=255)
                                                                            aCGTCACCAGCAGATAGCAACCGAGGCGCTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAACAGTGGTATCAAACGTGACGCGATCGCCGGGCCGAATTCCCGCCTGCATCACTTCGTCATCGGAGCGCGCAcc                                                                         >  2:2110403/1‑148 (MQ=255)
                                                                             cGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCa                                                                        >  2:3328741/1‑148 (MQ=255)
                                                                               tCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAAt                                                                      >  2:1601240/1‑148 (MQ=255)
                                                                                cACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATg                                                                     >  1:3224862/1‑148 (MQ=255)
                                                                                  ccAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTc                                                                   <  2:1679264/148‑1 (MQ=255)
                                                                                   cagcagATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTcc                                                                  <  2:80026/148‑1 (MQ=255)
                                                                                     gcagATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCAc                                                                >  2:1461473/1‑148 (MQ=255)
                                                                                     gcagATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCAc                                                                >  2:1461536/1‑148 (MQ=255)
                                                                                      cagATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCAcg                                                               >  1:2856996/1‑148 (MQ=255)
                                                                                      cagATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCAGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCAcg                                                               >  1:388373/1‑148 (MQ=255)
                                                                                        gATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCAcgcg                                                             >  2:945266/1‑148 (MQ=255)
                                                                                        gATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCAcgcg                                                             >  1:3524133/1‑148 (MQ=255)
                                                                                         aTAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACgcgc                                                            >  2:2404361/1‑148 (MQ=255)
                                                                                             cAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATgg                                                        <  1:2026336/148‑1 (MQ=255)
                                                                                               aCCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGTAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGgcg                                                      >  1:1812264/1‑148 (MQ=255)
                                                                                               aCCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGgcg                                                      >  2:1415445/1‑148 (MQ=255)
                                                                                                 cGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCt                                                    <  2:3506795/148‑1 (MQ=255)
                                                                                                   aGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGa                                                  <  1:455287/148‑1 (MQ=255)
                                                                                                   aGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGa                                                  >  1:3421885/1‑148 (MQ=255)
                                                                                                    ggCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGAc                                                 >  2:243507/1‑148 (MQ=255)
                                                                                                     gCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACg                                                <  2:385702/148‑1 (MQ=255)
                                                                                                       ggTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTc                                              <  2:2037995/148‑1 (MQ=255)
                                                                                                         tcatcaAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCAt                                            <  2:2294515/148‑1 (MQ=255)
                                                                                                           atcaAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTc                                          >  2:2784281/1‑148 (MQ=255)
                                                                                                           atcaAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTc                                          >  2:1676773/1‑148 (MQ=255)
                                                                                                             caAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCAGAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCCTTccc                                        >  2:189028/1‑148 (MQ=255)
                                                                                                              aaaGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTcccc                                       >  2:980676/1‑148 (MQ=255)
                                                                                                                 ggCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGc                                    <  1:1052874/148‑1 (MQ=255)
                                                                                                                 ggCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGc                                    >  1:1727245/1‑148 (MQ=255)
                                                                                                                 ggCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGc                                    >  1:2836756/1‑148 (MQ=255)
                                                                                                                 ggCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGc                                    >  1:2836840/1‑148 (MQ=255)
                                                                                                                   cTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGCCg                                  <  1:3328741/148‑1 (MQ=255)
                                                                                                                     ttCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGCCGGt                                >  1:1067880/1‑148 (MQ=255)
                                                                                                                        cccATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGCCGGTCGc                             <  2:1061547/148‑1 (MQ=255)
                                                                                                                        cccATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGCCGGTCGc                             <  1:284678/148‑1 (MQ=255)
                                                                                                                        cccATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGCCGGTCGc                             <  1:2108914/148‑1 (MQ=255)
                                                                                                                        cccATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGCCGGTCGc                             <  1:2107687/148‑1 (MQ=255)
                                                                                                                         ccATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGCCGGTCGcc                            >  1:112782/1‑148 (MQ=255)
                                                                                                                          cATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGCCGGTCGCCg                           >  2:11038/1‑148 (MQ=255)
                                                                                                                          cATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGCCGGTCGCCg                           <  2:2380867/148‑1 (MQ=255)
                                                                                                                          cATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGCCGGTCGCCg                           >  2:610135/1‑148 (MQ=255)
                                                                                                                             cACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGCCGGTCGCCGTCa                        <  2:3010442/148‑1 (MQ=255)
                                                                                                                              aCTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGCCGGTCGCCGTCaa                       <  2:20078/148‑1 (MQ=255)
                                                                                                                               cTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGCCGGTCGCCGTCAAg                      >  1:868707/1‑148 (MQ=255)
                                                                                                                               cTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGCCGGTCGCCGTCAAg                      >  1:1390731/1‑148 (MQ=255)
                                                                                                                                 cGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGCCGGTCGCCGTCAAGCa                    >  1:78125/1‑148 (MQ=255)
                                                                                                                                      gTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGCCGGTCGCCGTCAAGCAGGCCt               >  1:50573/1‑148 (MQ=255)
                                                                                                                                      gTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGCCGGTCGCCGTCAAGCAGGCCt               <  2:2773925/148‑1 (MQ=255)
                                                                                                                                           ggAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGCCGGTCGCCGTCAAGCAGGCCTGGAAt          >  1:3282055/1‑148 (MQ=255)
                                                                                                                                            gagaACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGCCGGTCGCCGTCAAGCAGGCCTGGAAtt         <  1:3162757/148‑1 (MQ=255)
                                                                                                                                                   tGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGCCGGTCGCCGTCAAGCAGGCCTGGAATTTTGCAct  <  2:2010224/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGCCGGTCGCCGTCAAGCAGGCCTGGAATTTTGCACT  >  CP009273/2498422‑2498716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑