Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 233,829 C→A 36.8% S3* (TCA→TAA)  yafT → lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273233,8290CA36.8% 309.9 / 209.5 223S3* (TCA→TAA) yafTlipoprotein
Reads supporting (aligned to +/- strand):  ref base C (82/59);  new base A (49/33);  total (131/92)
Fisher's exact test for biased strand distribution p-value = 8.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.38e-01

Only 100 of 228 total aligned reads displayed.

GAATAAATTTATATTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATT  >  CP009273/233684‑233975
                                                                                                                                                 |                                                                                                                                                  
gaATAAATTTATATTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCaa                                                                                                                                                  <  2:3270208/148‑1 (MQ=255)
 aaTAAATTTATATTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTaaaa                                                                                                                                                 >  1:2444090/1‑148 (MQ=255)
 aaTAAATTTATATTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTaaaa                                                                                                                                                 >  2:1008717/1‑148 (MQ=255)
  aTAAATTTATATTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAaaaa                                                                                                                                                >  1:1399432/1‑148 (MQ=255)
   tAAATTTATATTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCaaaaa                                                                                                                                               >  1:617278/1‑148 (MQ=255)
    aaaTTTATATTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCACTGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCaaaaaa                                                                                                                                              >  2:1051881/1‑148 (MQ=255)
    aaaTTTATATTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCaaaaaa                                                                                                                                              >  2:1774991/1‑148 (MQ=255)
     aaTTTATATTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAg                                                                                                                                             <  1:2031623/148‑1 (MQ=255)
      aTTTATATTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGc                                                                                                                                            <  1:498054/148‑1 (MQ=255)
       tttATATTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCt                                                                                                                                           >  2:1507033/1‑148 (MQ=255)
        ttATATTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCtt                                                                                                                                          <  1:2967028/148‑1 (MQ=255)
        ttATATTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCtt                                                                                                                                          >  1:1086141/1‑148 (MQ=255)
           tatTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTttgt                                                                                                                                       <  2:2738167/148‑1 (MQ=255)
             ttGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATTAATTAAAAAAAGATttgttg                                                                                                                                     >  2:3021481/1‑148 (MQ=255)
              tGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGc                                                                                                                                    >  2:2471948/1‑148 (MQ=255)
              tGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGc                                                                                                                                    <  1:3453946/148‑1 (MQ=255)
              tGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGc                                                                                                                                    <  1:2180293/148‑1 (MQ=255)
               gATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCa                                                                                                                                   >  1:2931028/1‑148 (MQ=255)
                 ttAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCata                                                                                                                                 >  2:1248042/1‑148 (MQ=255)
                 ttAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCata                                                                                                                                 <  1:3513248/148‑1 (MQ=255)
                    aatgaatgTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATAtgt                                                                                                                              <  2:2129667/148‑1 (MQ=255)
                     atgaatgTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATAtgtg                                                                                                                             >  1:2249202/1‑148 (MQ=255)
                         atgTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGtt                                                                                                                         >  1:1030058/1‑148 (MQ=255)
                           gTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTAt                                                                                                                       >  2:3488467/1‑148 (MQ=255)
                           gTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTAt                                                                                                                       >  2:1950172/1‑148 (MQ=255)
                               atTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATtctc                                                                                                                   <  1:1226008/148‑1 (MQ=255)
                                tttCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCg                                                                                                                  <  2:1872521/148‑1 (MQ=255)
                                 ttCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCgc                                                                                                                 >  2:2947459/1‑148 (MQ=255)
                                   cAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCgctg                                                                                                               >  1:190124/1‑148 (MQ=255)
                                   cAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCgctg                                                                                                               <  1:3175658/148‑1 (MQ=255)
                                    aaaTTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCgctgc                                                                                                              <  1:330175/148‑1 (MQ=255)
                                       ttGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTg                                                                                                           <  1:910487/148‑1 (MQ=255)
                                        tGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGc                                                                                                          <  1:2553119/148‑1 (MQ=255)
                                             ttgtttgtTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAACTATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGAt                                                                                                     <  2:2276782/148‑1 (MQ=255)
                                                tttgttATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGAtgtg                                                                                                  >  2:1404382/1‑148 (MQ=255)
                                                tttgttAATATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGAtgtg                                                                                                  >  2:1528568/1‑148 (MQ=255)
                                                  tgttATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGcc                                                                                                <  2:2076053/148‑1 (MQ=255)
                                                       ttattTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGa                                                                                           <  1:2170442/148‑1 (MQ=255)
                                                         attTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAAt                                                                                         <  2:394005/148‑1 (MQ=255)
                                                          tttAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAAtc                                                                                        >  2:3265732/1‑148 (MQ=255)
                                                          tttAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAAtc                                                                                        >  1:3545181/1‑148 (MQ=255)
                                                           ttAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAAtct                                                                                       >  1:1327876/1‑148 (MQ=255)
                                                            tAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAAtctt                                                                                      >  2:603981/1‑148 (MQ=255)
                                                              aGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAAtcttct                                                                                    <  2:2585107/148‑1 (MQ=255)
                                                              aGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAAtcttct                                                                                    >  2:2340928/1‑148 (MQ=255)
                                                               gTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTa                                                                                   <  2:2641743/148‑1 (MQ=255)
                                                               gTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTa                                                                                   <  2:1024614/148‑1 (MQ=255)
                                                               gTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTa                                                                                   >  2:935642/1‑148 (MQ=255)
                                                                tGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTAt                                                                                  <  2:1489180/148‑1 (MQ=255)
                                                                  aGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATtg                                                                                >  1:2631953/1‑148 (MQ=255)
                                                                  aGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATtg                                                                                >  1:541119/1‑148 (MQ=255)
                                                                  aGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATtg                                                                                >  1:2329517/1‑148 (MQ=255)
                                                                   ggTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGTGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATtga                                                                               <  1:1765843/148‑1 (MQ=255)
                                                                         taATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGaaaa                                                                         >  1:2850402/1‑148 (MQ=255)
                                                                           aTTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAaaga                                                                       <  1:667727/148‑1 (MQ=255)
                                                                            ttAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAaagaa                                                                      >  2:19362/1‑148 (MQ=255)
                                                                            ttAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAaagaa                                                                      >  2:19313/1‑148 (MQ=255)
                                                                               gagTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAgaagaa                                                                   >  2:3541236/1‑148 (MQ=255)
                                                                                  tCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGaaaaa                                                                >  1:3519255/1‑148 (MQ=255)
                                                                                  tCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGaaaaa                                                                <  1:1296295/148‑1 (MQ=255)
                                                                                  tCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGaaaaa                                                                >  1:2676814/1‑148 (MQ=255)
                                                                                      ttATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCt                                                            <  2:1259104/148‑1 (MQ=255)
                                                                                      ttATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCt                                                            <  1:250537/148‑1 (MQ=255)
                                                                                      ttATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCt                                                            >  1:1139179/1‑148 (MQ=255)
                                                                                       tATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTc                                                           >  1:1738919/1‑148 (MQ=255)
                                                                                         tCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCaa                                                         >  1:16658/1‑148 (MQ=255)
                                                                                           aaTGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGt                                                       >  1:2652010/1‑148 (MQ=255)
                                                                                             tGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTca                                                     <  1:3195697/148‑1 (MQ=255)
                                                                                              gCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTcac                                                    <  1:767636/148‑1 (MQ=255)
                                                                                                tAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTcacac                                                  >  2:3139522/1‑148 (MQ=255)
                                                                                                   atatTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACaaa                                               <  2:16658/148‑1 (MQ=255)
                                                                                                   atatTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACaaa                                               >  1:1564520/1‑148 (MQ=255)
                                                                                                    tatTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAg                                              <  1:3091023/148‑1 (MQ=255)
                                                                                                    aatTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCTCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGACACACAAAg                                              >  2:635561/2‑148 (MQ=255)
                                                                                                           aTCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATAtt                                       >  2:89734/1‑148 (MQ=255)
                                                                                                                tATGACAGGCGAGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATtaataa                                  >  2:3345859/1‑148 (MQ=255)
                                                                                                                    aCAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAac                              >  2:1904143/1‑148 (MQ=255)
                                                                                                                     cAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTAGTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATAAAAaca                             <  2:2792179/148‑1 (MQ=255)
                                                                                                                      aGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAacac                            <  1:3077428/148‑1 (MQ=255)
                                                                                                                        gCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTc                          >  2:1268482/1‑148 (MQ=255)
                                                                                                                         cGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTcc                         <  1:3436188/148‑1 (MQ=255)
                                                                                                                          gAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTccc                        <  2:1587857/148‑1 (MQ=255)
                                                                                                                           aGGGAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTcccc                       <  2:1843588/148‑1 (MQ=255)
                                                                                                                            gggAGTGTCCAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCa                      >  2:3368596/1‑148 (MQ=255)
                                                                                                                              gAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGc                    >  2:467940/1‑148 (MQ=255)
                                                                                                                              gAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATAAAAACACTCCCCAGc                    >  1:1079768/1‑148 (MQ=255)
                                                                                                                              gAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATAAAAACACTCCCCAGc                    >  1:2854166/1‑148 (MQ=255)
                                                                                                                              gAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATAAAAACACTCCCCAGc                    >  1:3232859/1‑148 (MQ=255)
                                                                                                                                    ccAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATAAAAACACTCCCCAGCAACTga              >  1:811649/1‑148 (MQ=255)
                                                                                                                                    ccAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAATAATATTAATAAAAACACTCCCCAGCAACTga              >  1:2594038/1‑148 (MQ=255)
                                                                                                                                    ccAATTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTga              >  1:1491314/1‑148 (MQ=255)
                                                                                                                                      aaTTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTgaca            <  1:3053457/148‑1 (MQ=255)
                                                                                                                                       aTTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTgacag           >  1:1882299/1‑148 (MQ=255)
                                                                                                                                       aTTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTgacag           <  2:2437548/148‑1 (MQ=255)
                                                                                                                                       aTTATGAATTAAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTgacag           <  1:371009/148‑1 (MQ=255)
                                                                                                                                        ttATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTgacaga          >  1:1614432/1‑148 (MQ=255)
                                                                                                                                        ttATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTgacaga          >  2:485100/1‑148 (MQ=255)
                                                                                                                                        ttATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTgacaga          <  1:3488467/148‑1 (MQ=255)
                                                                                                                                          aTGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTgacagaca        <  1:2228821/148‑1 (MQ=255)
                                                                                                                                              aTTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGa    <  1:484507/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GAATAAATTTATATTGATTAAATGAATGTATATTTCAAATTGATTTTGTTTGTTATTATTTAAGTGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATT  >  CP009273/233684‑233975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑