Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,512,279 G→T 8.6% D112Y (GAT→TAT)  yfeC → DUF1323 family putative DNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,512,2790GT 8.6% 786.0 / 31.9 220D112Y (GAT→TAT) yfeCDUF1323 family putative DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (114/87);  new base T (4/15);  total (118/102)
Fisher's exact test for biased strand distribution p-value = 3.43e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

Only 100 of 228 total aligned reads displayed.

GAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAAAAATTTCCCGGCGTGAAAGGCGGTCG  >  CP009273/2512132‑2512425
                                                                                                                                                   |                                                                                                                                                  
 aaGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGa                                                                                                                                                   <  1:1553981/148‑1 (MQ=255)
  aGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTcgcg                                                                                                                                                    >  1:2082673/1‑146 (MQ=255)
  aGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTcgcg                                                                                                                                                    <  2:278571/146‑1 (MQ=255)
  aGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGAt                                                                                                                                                  <  2:273883/148‑1 (MQ=255)
  aGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCGTAGGGATTCGCGAt                                                                                                                                                  >  1:3338690/1‑148 (MQ=255)
  aGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGAAACTGGCGAAAGAGATGACGACAGTTGATCAAAAACAGTTTACATCCTTGCTTCTGCGTGAAGGGATTATCGTATTGTTACAACGCTTAGGGATTCGCGAt                                                                                                                                                  <  2:2849372/148‑1 (MQ=255)
   gcgcCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATa                                                                                                                                                 >  2:1300534/1‑148 (MQ=255)
    cgcCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAg                                                                                                                                                >  2:1100861/1‑148 (MQ=255)
    cgcCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAg                                                                                                                                                >  2:3248560/1‑148 (MQ=255)
       cTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCaa                                                                                                                                             <  1:1117138/148‑1 (MQ=255)
         gCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAaat                                                                                                                                           <  2:3500980/148‑1 (MQ=255)
         gCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAaat                                                                                                                                           <  2:2706603/148‑1 (MQ=255)
           ccTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAaataa                                                                                                                                         >  2:3479628/1‑148 (MQ=255)
             tttCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAataata                                                                                                                                       >  2:506835/1‑148 (MQ=255)
             tttCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAataata                                                                                                                                       >  1:1686394/1‑148 (MQ=255)
             tttCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAataata                                                                                                                                       <  1:163298/148‑1 (MQ=255)
                 tGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGaa                                                                                                                                   <  2:3499120/148‑1 (MQ=255)
                 tGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCAAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGaa                                                                                                                                   <  1:2222002/148‑1 (MQ=255)
                  tgTGATGCGCCGCTTGAATTATTTATGGTGAGACTTTCGAAAGATATGACGACAGTTGAACAAAAACAGTTTACATGCTTGCTTCTTCGGGAAGGGATTATCGGATTGTTTCATCGATTAGGGTTTCTCGATAGCTAATAATATGata                                                                                                                                  <  2:2466544/147‑3 (MQ=255)
                  ggTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGaaa                                                                                                                                  >  1:1913467/1‑148 (MQ=255)
                    tGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAg                                                                                                                                >  1:2076354/1‑148 (MQ=255)
                     gATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGa                                                                                                                               >  1:890870/1‑148 (MQ=255)
                     gATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGa                                                                                                                               <  1:2819398/148‑1 (MQ=255)
                     gATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGa                                                                                                                               >  1:2715398/1‑148 (MQ=255)
                     gATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGCTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGa                                                                                                                               <  1:2163720/148‑1 (MQ=255)
                       tGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGAtt                                                                                                                             <  2:1497965/148‑1 (MQ=255)
                       tGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGAtt                                                                                                                             <  2:2919720/148‑1 (MQ=255)
                           ccgcTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGc                                                                                                                         >  2:888007/1‑148 (MQ=255)
                             gcTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCaa                                                                                                                       <  1:1111158/148‑1 (MQ=255)
                                  aaGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATaaaa                                                                                                                  <  1:472392/148‑1 (MQ=255)
                                       ttGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAATTAATATGAAAAGATTACGCAATAAAATGacc                                                                                                             >  2:2083113/1‑148 (MQ=255)
                                        tGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGacca                                                                                                            <  2:493440/148‑1 (MQ=255)
                                         gCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGaccac                                                                                                           <  2:570941/148‑1 (MQ=255)
                                             gTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCgaa                                                                                                       >  2:1465425/1‑148 (MQ=255)
                                                 cacTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAAc                                                                                                   >  1:822283/1‑148 (MQ=255)
                                                   cTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTg                                                                                                 <  1:1239978/148‑1 (MQ=255)
                                                   cTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTg                                                                                                 <  2:1171181/148‑1 (MQ=255)
                                                   cTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTg                                                                                                 <  1:3287457/148‑1 (MQ=255)
                                                      gCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCt                                                                                              <  2:2554899/148‑1 (MQ=255)
                                                         aaaGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGaa                                                                                           <  2:2980033/148‑1 (MQ=255)
                                                          aaGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAAt                                                                                          >  2:3356561/1‑148 (MQ=255)
                                                          aaGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAAt                                                                                          >  2:1029123/1‑148 (MQ=255)
                                                             agaTGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGcc                                                                                       <  1:1474170/148‑1 (MQ=255)
                                                              gaTGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCt                                                                                      <  2:1913467/148‑1 (MQ=255)
                                                                tGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATTCCTCg                                                                                    >  2:2052078/1‑148 (MQ=255)
                                                                tGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCg                                                                                    >  2:2580256/1‑148 (MQ=255)
                                                                  aCGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGgt                                                                                  >  2:3543878/1‑148 (MQ=255)
                                                                  aCGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGgt                                                                                  <  1:462641/148‑1 (MQ=255)
                                                                   cGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGgtg                                                                                 <  1:1184365/148‑1 (MQ=255)
                                                                     ccAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGtgtg                                                                               <  1:2518321/148‑1 (MQ=255)
                                                                     ccAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGtgtg                                                                               <  1:735900/148‑1 (MQ=255)
                                                                           gAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTaaa                                                                         >  2:1699274/1‑148 (MQ=255)
                                                                           gAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTaaa                                                                         <  1:3479628/148‑1 (MQ=255)
                                                                             aCAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTaaaca                                                                       >  1:3162608/1‑148 (MQ=255)
                                                                             aCAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTaaaca                                                                       >  2:3193714/1‑148 (MQ=255)
                                                                               aaaaaCAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCTATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTaaacaaa                                                                     <  2:2341039/148‑1 (MQ=255)
                                                                                 aaaCAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACt                                                                   >  2:3043479/1‑148 (MQ=255)
                                                                                  aaCAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTg                                                                  >  2:1424962/1‑148 (MQ=255)
                                                                                    cAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGtt                                                                >  1:2025473/1‑148 (MQ=255)
                                                                                     aGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTa                                                               >  2:864220/1‑148 (MQ=255)
                                                                                       tttACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAAc                                                             >  1:618627/1‑148 (MQ=255)
                                                                                       tttACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAAc                                                             >  2:2679376/1‑148 (MQ=255)
                                                                                         tACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCg                                                           <  1:191734/148‑1 (MQ=255)
                                                                                          aCATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGt                                                          <  2:251405/148‑1 (MQ=255)
                                                                                            aTCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTg                                                        >  2:1339601/1‑148 (MQ=255)
                                                                                             tCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTgg                                                       >  1:1664758/1‑148 (MQ=255)
                                                                                              ccTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGa                                                      <  1:1424885/148‑1 (MQ=255)
                                                                                              ccTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGa                                                      <  1:922611/148‑1 (MQ=255)
                                                                                              ccTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGa                                                      >  1:2539993/1‑148 (MQ=255)
                                                                                                 tGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCa                                                   >  1:232712/1‑148 (MQ=255)
                                                                                                 tGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCa                                                   >  1:529076/1‑148 (MQ=255)
                                                                                                      cTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGaa                                              >  2:2232360/1‑148 (MQ=255)
                                                                                                       tGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAATGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGAGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGaaa                                             >  1:1028370/1‑148 (MQ=255)
                                                                                                         cGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGaaaaa                                           <  2:474481/148‑1 (MQ=255)
                                                                                                           ggAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAgg                                         <  1:1232495/148‑1 (MQ=255)
                                                                                                           ggAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAgg                                         <  2:2830843/148‑1 (MQ=255)
                                                                                                           ggAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAgg                                         >  1:1907225/1‑148 (MQ=255)
                                                                                                           ggAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAgg                                         >  2:2437754/1‑148 (MQ=255)
                                                                                                           ggAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAgg                                         <  1:3005532/148‑1 (MQ=255)
                                                                                                            gAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTCAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGc                                        >  2:2737497/1‑148 (MQ=255)
                                                                                                            gAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGc                                        >  1:1861755/1‑148 (MQ=255)
                                                                                                            gAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGc                                        >  1:113772/1‑148 (MQ=255)
                                                                                                             aaGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCt                                       >  1:954005/1‑148 (MQ=255)
                                                                                                                ggATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGa                                    >  1:927685/1‑148 (MQ=255)
                                                                                                                   ttATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGaaaa                                 >  2:1544851/1‑148 (MQ=255)
                                                                                                                          aTTGTTACAAAGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGaaaa                          <  1:784218/148‑1 (MQ=255)
                                                                                                                           ttGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGaaaaa                         >  2:2013728/1‑148 (MQ=255)
                                                                                                                            tGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAAAAAt                        >  1:2462322/1‑148 (MQ=255)
                                                                                                                            tGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAAAAAt                        >  2:1792657/1‑148 (MQ=255)
                                                                                                                             gTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAAAAAtt                       <  2:2881481/148‑1 (MQ=255)
                                                                                                                              ttACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAAAAAttt                      >  1:2890833/1‑148 (MQ=255)
                                                                                                                              ttACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAAAAAttt                      <  2:3338690/148‑1 (MQ=255)
                                                                                                                              ttACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAAAAAttt                      >  2:3421052/1‑148 (MQ=255)
                                                                                                                                           ggATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAAAAATTTCCCGGCGTGAAAg         >  2:1382914/1‑148 (MQ=255)
                                                                                                                                           ggATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAAAAATTTCCCGGCGTGAAAg         >  2:3161476/1‑148 (MQ=255)
                                                                                                                                             aTTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAAAAATTTCCCGGCGTGAAAGGc       >  2:1541070/1‑148 (MQ=255)
                                                                                                                                              ttCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAAAAATTTCCCGGCGTGAAAGGCg      <  2:3090215/148‑1 (MQ=255)
                                                                                                                                               tCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAAAAATTTCCCGGCGTGAAAGGCgg     >  2:343710/1‑148 (MQ=255)
                                                                                                                                               tCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAAAAATTTCCCGGCGTGAAAGGCgg     <  1:1743938/148‑1 (MQ=255)
                                                                                                                                                  cgATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAAAAATTTCCCGGCGTGAAAGGCGGTCg  >  1:3027170/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTTCTGCGGGAAGGGATTATCGGATTGTTACAACGCTTAGGGATTCGCGATAGCAAATAATATGAAAAGATTACGCAATAAAATGACCACCGAAGAACTGGCTGAATGCCTCGGTGTGGCTAAACAAACTGTTAACCGTTGGATCAGAGAAAAAGGCTGGAAAACGGAAAAATTTCCCGGCGTGAAAGGCGGTCG  >  CP009273/2512132‑2512425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑