BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,524,056	C→A	94.5%	A179A (GCG→GCT)	zipA ←	FtsZ stabilizer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,524,056	0	C	A	94.5%	507.1 / 18.6	174	A179A (GCG→GCT)	zipA	FtsZ stabilizer
Reads supporting (aligned to +/- strand): ref base C (3/7); new base A (87/77); total (90/84)
Fisher's exact test for biased strand distribution p-value = 2.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 174 total aligned reads displayed.

TATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGG  >  CP009273/2523911‑2524199

tatTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGc                                                                                                                                               <  2:1097217/148‑1 (MQ=255)

   tCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTAc                                                                                                                                            >  1:1233080/1‑148 (MQ=255)

    cATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTAca                                                                                                                                           >  1:46704/1‑148 (MQ=255)

       atCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTAcaggc                                                                                                                                        <  2:1021287/148‑1 (MQ=255)

        tCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTAcaggct                                                                                                                                       >  1:1289590/1‑148 (MQ=255)

        tCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTAcaggct                                                                                                                                       >  1:1289862/1‑148 (MQ=255)

        tCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTAcaggct                                                                                                                                       >  1:1414620/1‑148 (MQ=255)

          gCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTAcaggctca                                                                                                                                     >  1:690626/1‑148 (MQ=255)

              aaaaTGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTAcaggctcaggct                                                                                                                                 >  2:467581/1‑148 (MQ=255)

              aaaaTGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTAcaggctcaggct                                                                                                                                 <  2:1103935/148‑1 (MQ=255)

               aaaTGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCtg                                                                                                                                >  2:1661582/1‑148 (MQ=255)

               aaaTGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCtg                                                                                                                                <  1:916870/148‑1 (MQ=255)

                aaTGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCtgt                                                                                                                               <  2:2282084/148‑1 (MQ=255)

                    aaGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTgtggtg                                                                                                                           <  1:653882/148‑1 (MQ=255)

                           cTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTAc                                                                                                                    >  2:859845/1‑148 (MQ=255)

                            ttgttgAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACg                                                                                                                   <  1:883445/148‑1 (MQ=255)

                             tgttgAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATCATAATCACCGCTTCTTTGCGCGTCGGTTTATCCATAACAGGAGCAGGTTCCGCAACAGGCTCAGGCTGGGGTGCCGCTACgg                                                                                                                  >  2:213669/1‑148 (MQ=255)

                              gttgAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGATTCTTTGCGCTTCGGTTTATCCATAACTGGGGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACggg                                                                                                                 <  2:3349037/148‑1 (MQ=255)

                                tgAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGtt                                                                                                               >  2:3540064/1‑148 (MQ=255)

                                 gAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTc                                                                                                              <  2:2871682/148‑1 (MQ=255)

                                       tGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAgg                                                                                                        <  1:1384542/148‑1 (MQ=255)

                                        gTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGc                                                                                                       >  2:1211520/1‑148 (MQ=255)

                                        gTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGc                                                                                                       >  2:1211546/1‑148 (MQ=255)

                                        gTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGc                                                                                                       >  2:1494006/1‑148 (MQ=255)

                                              aagCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGaaa                                                                                                 >  1:685818/1‑148 (MQ=255)

                                                gCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGc                                                                                               <  2:2018677/148‑1 (MQ=255)

                                                gCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGc                                                                                               <  2:2019207/148‑1 (MQ=255)

                                                 cAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGcc                                                                                              >  1:761655/1‑148 (MQ=255)

                                                 cAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGcc                                                                                              >  1:761637/1‑148 (MQ=255)

                                                      cACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCtgttg                                                                                         <  2:1223489/148‑1 (MQ=255)

                                                           tttAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCg                                                                                    >  2:42803/1‑148 (MQ=255)

                                                             tAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGt                                                                                  >  2:578154/1‑148 (MQ=255)

                                                                cTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGc                                                                               >  1:1131093/1‑148 (MQ=255)

                                                                  cGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCgg                                                                             >  2:1505171/1‑148 (MQ=255)

                                                                  cGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCgg                                                                             >  2:1505547/1‑148 (MQ=255)

                                                                  cGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCgg                                                                             <  2:2797203/148‑1 (MQ=255)

                                                                  cGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCgg                                                                             <  1:1797038/148‑1 (MQ=255)

                                                                  cGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCgg                                                                             <  2:2797970/148‑1 (MQ=255)

                                                                    cTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTg                                                                           <  2:2535397/148‑1 (MQ=255)

                                                                     tACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGc                                                                          <  2:8836/148‑1 (MQ=255)

                                                                      aCCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTAGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCt                                                                         >  1:1173069/1‑148 (MQ=255)

                                                                       ccGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTg                                                                        >  1:2846976/1‑148 (MQ=255)

                                                                              gcgcCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGcac                                                                 <  1:3178381/148‑1 (MQ=255)

                                                                               cgccgcGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGcaca                                                                <  1:615773/148‑1 (MQ=255)

                                                                                 ccgcGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAgg                                                              <  1:663754/148‑1 (MQ=255)

                                                                                 ccgcGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAgg                                                              >  1:833613/1‑148 (MQ=255)

                                                                                 ccgcGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAgg                                                              >  1:398694/1‑148 (MQ=255)

                                                                                  cgcgACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGc                                                             <  2:1482844/148‑1 (MQ=255)

                                                                                    cgACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTg                                                           <  1:203201/148‑1 (MQ=255)

                                                                                    cgACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTg                                                           <  2:1442992/148‑1 (MQ=255)

                                                                                    cgACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTg                                                           <  2:739494/148‑1 (MQ=255)

                                                                                      aCGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTgcg                                                         >  2:2628638/1‑148 (MQ=255)

                                                                                         ttCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTgcggcg                                                      <  2:1782826/148‑1 (MQ=255)

                                                                                         ttCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTgcggcg                                                      <  1:2932252/148‑1 (MQ=255)

                                                                                          tCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGgcgc                                                     >  1:1581051/1‑148 (MQ=255)

                                                                                              gATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGgc                                                 >  1:1797271/1‑148 (MQ=255)

                                                                                                   tCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGAc                                            >  2:2909569/1‑148 (MQ=255)

                                                                                                   tCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGAc                                            >  1:168679/1‑148 (MQ=255)

                                                                                                   tCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGAc                                            >  2:2564309/1‑148 (MQ=255)

                                                                                                      ccGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCtg                                         <  2:2511932/148‑1 (MQ=255)

                                                                                                       cGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCtgt                                        >  1:2229693/1‑148 (MQ=255)

                                                                                                       cGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCtgt                                        >  1:2560888/1‑148 (MQ=255)

                                                                                                           tcttTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCg                                    >  2:2300824/1‑148 (MQ=255)

                                                                                                           tcttTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCg                                    >  2:1963494/1‑148 (MQ=255)

                                                                                                             tttGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGaa                                  >  2:2386265/1‑148 (MQ=255)

                                                                                                                gcgcTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACt                               >  1:501780/1‑148 (MQ=255)

                                                                                                                 cgcTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTg                              <  1:515754/148‑1 (MQ=255)

                                                                                                                 cgcTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTg                              <  1:2754263/148‑1 (MQ=255)

                                                                                                                  gcTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTgg                             <  1:195174/148‑1 (MQ=255)

                                                                                                                  gcTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTgg                             <  1:1108819/148‑1 (MQ=255)

                                                                                                                  gcTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTgg                             <  2:1151538/148‑1 (MQ=255)

                                                                                                                   cTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGgc                            <  2:820653/148‑1 (MQ=255)

                                                                                                                   cTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGgc                            >  1:1510442/1‑148 (MQ=255)

                                                                                                                    ttCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGgct                           >  1:206803/1‑148 (MQ=255)

                                                                                                                     tCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGgctg                          <  2:3355691/148‑1 (MQ=255)

                                                                                                                         tttATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCtgctgc                      >  1:523921/1‑148 (MQ=255)

                                                                                                                          ttATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCa                     <  2:1558107/148‑1 (MQ=255)

                                                                                                                          ttATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCa                     >  1:843371/1‑148 (MQ=255)

                                                                                                                                  aaCTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGt             >  1:2706128/1‑148 (MQ=255)

                                                                                                                                  aaCTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTCGCTGCTGCAACGGCTGt             >  1:2039717/1‑148 (MQ=255)

                                                                                                                                   aCTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGACAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGtt            <  2:2921134/148‑1 (MQ=255)

                                                                                                                                   aCTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGtt            <  1:2646549/148‑1 (MQ=255)

                                                                                                                                   aCTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGtt            >  1:3441719/1‑148 (MQ=255)

                                                                                                                                   aCTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGtt            >  2:3445998/1‑148 (MQ=255)

                                                                                                                                    cTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTc           >  2:3191131/1‑148 (MQ=255)

                                                                                                                                    cTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTc           <  1:868050/148‑1 (MQ=255)

                                                                                                                                    cTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTc           <  2:1292456/148‑1 (MQ=255)

                                                                                                                                    cTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTc           <  2:3012173/148‑1 (MQ=255)

                                                                                                                                    cTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTc           >  2:2981503/1‑148 (MQ=255)

                                                                                                                                    cTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTc           <  1:16024/148‑1 (MQ=255)

                                                                                                                                    cTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTc           <  1:2319096/148‑1 (MQ=255)

                                                                                                                                     tGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCa          >  1:1144209/1‑148 (MQ=255)

                                                                                                                                      ggAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAg         >  2:66345/1‑148 (MQ=255)

                                                                                                                                      ggAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTcgg         >  2:1694912/1‑146 (MQ=255)

                                                                                                                                      ggAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTcgg         >  2:1696230/1‑146 (MQ=255)

                                                                                                                                      ggAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAg         <  1:1055626/148‑1 (MQ=255)

                                                                                                                                      ggAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAg         <  1:1055360/148‑1 (MQ=255)

                                                                                                                                       gAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAgg        <  1:3370043/148‑1 (MQ=255)

                                                                                                                                         gCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCt      <  2:2230379/148‑1 (MQ=255)

                                                                                                                                             gTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCgg  <  2:1414620/148‑1 (MQ=255)

TATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGG  >  CP009273/2523911‑2524199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`