BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,531,188	C→A	5.4%	V27V (GTC→GTA)	yfeS →	WGR domain protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,531,188	0	C	A	5.4%	730.8 / 12.3	192	V27V (GTC→GTA)	yfeS	WGR domain protein
Reads supporting (aligned to +/- strand): ref base C (101/80); new base A (8/3); total (109/83)
Fisher's exact test for biased strand distribution p-value = 3.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 195 total aligned reads displayed.

GACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCATGGATCGC  >  CP009273/2531042‑2531335

gACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCa                                                                                                                                                    >  2:1944796/1‑148 (MQ=255)

 aCGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCaa                                                                                                                                                   <  2:2132247/148‑1 (MQ=255)

  cGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAAc                                                                                                                                                  <  1:1108738/148‑1 (MQ=255)

     aTAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTAt                                                                                                                                               >  1:2847829/1‑148 (MQ=255)

      tAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATg                                                                                                                                              >  1:1323584/1‑148 (MQ=255)

      tAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATg                                                                                                                                              <  2:115338/148‑1 (MQ=255)

       aaaaCGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATgg                                                                                                                                             >  1:87390/1‑148 (MQ=255)

             gTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAaggt                                                                                                                                       >  2:1008184/1‑148 (MQ=255)

               gCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAaggtag                                                                                                                                     <  1:2639014/148‑1 (MQ=255)

                    cGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGgtagt                                                                                                                                <  1:1043177/148‑1 (MQ=255)

                    cGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGgtagt                                                                                                                                >  1:591552/1‑148 (MQ=255)

                    cGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTAATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGgtagt                                                                                                                                >  1:592196/1‑148 (MQ=255)

                       aaggaaATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTAAACTATGGCAAGGTAGGTAGTAtt                                                                                                                             >  2:2842773/1‑148 (MQ=255)

                       aaggaaATTAATCCGCTTTGGGAAGGCATTAACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTAtt                                                                                                                             >  2:3415160/1‑148 (MQ=255)

                        aggaaATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTg                                                                                                                            <  1:719821/148‑1 (MQ=255)

                         ggaaATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTgg                                                                                                                           <  2:923614/148‑1 (MQ=255)

                          gaaATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGt                                                                                                                          <  1:2922079/148‑1 (MQ=255)

                           aaaTTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTa                                                                                                                         >  1:1080710/1‑148 (MQ=255)

                             aTTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTaaa                                                                                                                       >  1:3013819/1‑148 (MQ=255)

                               tAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAAtt                                                                                                                     <  1:2728555/148‑1 (MQ=255)

                                 aTCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTcc                                                                                                                   >  1:3214048/1‑148 (MQ=255)

                                   ccGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAg                                                                                                                 <  2:603669/148‑1 (MQ=255)

                                   ccGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAg                                                                                                                 <  1:2294529/148‑1 (MQ=255)

                                   ccGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTAAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAg                                                                                                                 <  1:1054285/148‑1 (MQ=255)

                                      cTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACa                                                                                                              >  1:381947/1‑148 (MQ=255)

                                        ttGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACaaa                                                                                                            >  1:357303/1‑148 (MQ=255)

                                          gggAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAag                                                                                                          <  1:3168436/148‑1 (MQ=255)

                                           ggAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAaga                                                                                                         >  2:1799825/1‑148 (MQ=255)

                                             aaGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGt                                                                                                       <  2:2446449/148‑1 (MQ=255)

                                               ggCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTc                                                                                                     >  2:1351708/1‑148 (MQ=255)

                                               ggCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTc                                                                                                     >  2:521835/1‑148 (MQ=255)

                                                 cATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGa                                                                                                   <  2:631154/148‑1 (MQ=255)

                                                 cATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGa                                                                                                   <  2:3396392/148‑1 (MQ=255)

                                                  aTTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGAt                                                                                                  >  1:1528991/1‑148 (MQ=255)

                                                   tttACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATa                                                                                                 <  2:1047875/148‑1 (MQ=255)

                                                     tACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAAt                                                                                               <  2:1852350/148‑1 (MQ=255)

                                                      aCAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATg                                                                                              >  2:2104763/1‑148 (MQ=255)

                                                       cAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATga                                                                                             >  1:1914426/1‑148 (MQ=255)

                                                        aggaggTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATgaa                                                                                            >  1:1218717/1‑148 (MQ=255)

                                                                aCATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAgtg                                                                                    <  1:3089806/148‑1 (MQ=255)

                                                                  aTGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTc                                                                                  <  1:3303022/148‑1 (MQ=255)

                                                                   tGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCt                                                                                 >  2:426772/1‑148 (MQ=255)

                                                                     aaaaaaCGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAAAAGTGTCTGa                                                                               >  1:2484575/1‑148 (MQ=255)

                                                                      aaaaaCGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGaa                                                                              <  1:2372883/148‑1 (MQ=255)

                                                                       aaaaCGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGaaa                                                                             <  1:2666879/148‑1 (MQ=255)

                                                                        aaaCGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAaag                                                                            >  2:737239/1‑148 (MQ=255)

                                                                        aaaCGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAaag                                                                            >  2:1498271/1‑148 (MQ=255)

                                                                        aaaCGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAaag                                                                            >  1:2429812/1‑148 (MQ=255)

                                                                           cGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAaagaag                                                                         >  2:1125786/1‑148 (MQ=255)

                                                                            gCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGc                                                                        <  2:255273/148‑1 (MQ=255)

                                                                             cTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGcc                                                                       >  1:1974075/1‑148 (MQ=255)

                                                                              tttatttaTCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCa                                                                      >  2:3114780/1‑148 (MQ=255)

                                                                              tttatttaTCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCa                                                                      <  2:3492591/148‑1 (MQ=255)

                                                                              tttatttaTCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATCCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCa                                                                      >  1:2284968/1‑148 (MQ=255)

                                                                               ttatttatCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAg                                                                     <  2:314752/148‑1 (MQ=255)

                                                                               ttatttatCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAg                                                                     >  1:278399/1‑148 (MQ=255)

                                                                                tatttatCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGt                                                                    >  2:1375947/1‑148 (MQ=255)

                                                                                tatttatCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTAAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGt                                                                    >  1:2690084/1‑148 (MQ=255)

                                                                                 atttatCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTAAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTa                                                                   <  2:2880717/148‑1 (MQ=255)

                                                                                  tttatCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTaa                                                                  <  2:865668/148‑1 (MQ=255)

                                                                                  tttatCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTaa                                                                  <  2:1215427/148‑1 (MQ=255)

                                                                                  tttatCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTAAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTaa                                                                  >  1:2284315/1‑148 (MQ=255)

                                                                                   ttatCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTaaa                                                                 >  1:1695793/1‑148 (MQ=255)

                                                                                   ttatCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTaaa                                                                 <  1:3377228/148‑1 (MQ=255)

                                                                                    tatCACGATGAAAAATAGAATAAATTTTGGTGGATAGATTACGAATGTGATAGTTTAGCTGTCAACTATGGCAAGGTATGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAat                                                                <  1:2098087/148‑1 (MQ=255)

                                                                                       cACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAattga                                                             >  2:1758458/1‑148 (MQ=255)

                                                                                            tGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTACAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGcc                                                        >  2:2484016/1‑148 (MQ=255)

                                                                                             gAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCg                                                       <  1:334735/148‑1 (MQ=255)

                                                                                             gAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCg                                                       >  2:2210839/1‑148 (MQ=255)

                                                                                                aaaTCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCaa                                                    >  1:1824935/1‑148 (MQ=255)

                                                                                                 aaTCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCaaa                                                   >  2:2694778/1‑148 (MQ=255)

                                                                                                  aTCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCaaaa                                                  >  2:2262547/1‑148 (MQ=255)

                                                                                                  aTCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCaaaa                                                  >  1:407571/1‑148 (MQ=255)

                                                                                                    cGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAAt                                                >  1:1413827/1‑148 (MQ=255)

                                                                                                      aaTAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATga                                              <  2:2630254/148‑1 (MQ=255)

                                                                                                         aaaTTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATgaaga                                           <  2:2383124/148‑1 (MQ=255)

                                                                                                         aaaTTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATgaaga                                           <  1:1338638/148‑1 (MQ=255)

                                                                                                            ttttGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAg                                        <  2:1323584/148‑1 (MQ=255)

                                                                                                             tttGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAgg                                       <  1:568422/148‑1 (MQ=255)

                                                                                                                ggtggATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTa                                    <  2:2190259/148‑1 (MQ=255)

                                                                                                                   ggATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCa                                 >  1:775472/1‑148 (MQ=255)

                                                                                                                   ggATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCa                                 >  2:2933823/1‑148 (MQ=255)

                                                                                                                    gATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCaa                                >  2:736150/1‑148 (MQ=255)

                                                                                                                    gATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCaa                                >  1:159245/1‑148 (MQ=255)

                                                                                                                        gATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCaagaag                            >  2:397190/1‑148 (MQ=255)

                                                                                                                         aTTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAagaaga                           <  2:155237/148‑1 (MQ=255)

                                                                                                                            aCGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATcc                        <  2:3209390/148‑1 (MQ=255)

                                                                                                                              gAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCaa                      >  1:2170783/1‑148 (MQ=255)

                                                                                                                               aaGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCaaa                     >  2:1081410/1‑148 (MQ=255)

                                                                                                                                   ggATAGTTTAGCAGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGttt                 <  2:1137703/148‑1 (MQ=255)

                                                                                                                                    gATAGTTTATCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTa                >  2:3093409/1‑148 (MQ=255)

                                                                                                                                    gATAGTTTATATGTAAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGAGTCTGAAATAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGAAATAAAGAATATCCAAAGTTTa                >  2:3094966/1‑148 (MQ=255)

                                                                                                                                     aTAGTTTAGCTGTAAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTACATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTaa               >  1:2831526/1‑148 (MQ=255)

                                                                                                                                         tttAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCGAAGTTTAACTTc           <  2:2845154/148‑1 (MQ=255)

                                                                                                                                           tAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCAt         <  1:2104763/148‑1 (MQ=255)

                                                                                                                                             gCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCATgg       >  1:578565/1‑148 (MQ=255)

                                                                                                                                              cTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCATGGa      <  1:1146278/148‑1 (MQ=255)

                                                                                                                                               tGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCAGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCATGGAt     >  2:1510381/1‑148 (MQ=255)

                                                                                                                                                 tCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCATGGATCg   <  1:1944796/148‑1 (MQ=255)

                                                                                                                                                  cAACTATGGCAAGGTAGGTAGTATTGGTAAATCCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCATGGATCGc  <  2:2284968/148‑1 (MQ=255)

GACGGATAAAACGGTGCCTGCGGAAGGAAATTAATCCGCTTTGGGAAGGCATTTACAGGAGGTAACATGAAAAAACGCTTTATTTATCACGATGAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAATTGATTGCCGCAAAAATGAAGAAAGGCTATCAAGAAGATCCAAAGTTTAACTTCATGGATCGC  >  CP009273/2531042‑2531335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`