Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,567,775 G→T 6.3% I196I (ATC→ATA)  eutQ ← RmlC‑like cupin domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,567,7750GT 6.3% 711.8 / 16.2 189I196I (ATC→ATA) eutQRmlC‑like cupin domain protein
Reads supporting (aligned to +/- strand):  ref base G (95/82);  new base T (6/6);  total (101/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 190 total aligned reads displayed.

TCGCTCTTAGCCATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCACAAG  >  CP009273/2567628‑2567921
                                                                                                                                                   |                                                                                                                                                  
 cGCTCTTAGCCATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGa                                                                                                                                                   >  2:2651806/1‑148 (MQ=255)
    tctTAGCCATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCa                                                                                                                                                <  2:3239758/148‑1 (MQ=255)
     ctTAGCCATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCAt                                                                                                                                               >  1:918853/1‑148 (MQ=255)
     ctTAGCCATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCAt                                                                                                                                               >  1:1838513/1‑148 (MQ=255)
      ttAGCCATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATg                                                                                                                                              >  1:2083826/1‑148 (MQ=255)
          ccATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGttt                                                                                                                                          <  2:1097838/148‑1 (MQ=255)
           cATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTg                                                                                                                                         >  1:2501265/1‑148 (MQ=255)
            aTGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTgg                                                                                                                                        >  2:243419/1‑148 (MQ=255)
            aTGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTgg                                                                                                                                        >  1:1296644/1‑148 (MQ=255)
            aTGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTgg                                                                                                                                        >  2:2271862/1‑148 (MQ=255)
             tGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGc                                                                                                                                       >  1:2697809/1‑148 (MQ=255)
              gCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGcc                                                                                                                                      >  1:3424840/1‑148 (MQ=255)
                ttCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCtt                                                                                                                                    >  1:1628420/1‑148 (MQ=255)
                ttCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCtt                                                                                                                                    >  1:2110974/1‑148 (MQ=255)
                   ggTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGt                                                                                                                                 <  2:1062806/148‑1 (MQ=255)
                   ggTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGt                                                                                                                                 <  2:331436/148‑1 (MQ=255)
                   ggTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGt                                                                                                                                 <  1:1763319/148‑1 (MQ=255)
                     tgatgaAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTgg                                                                                                                               <  2:3142034/148‑1 (MQ=255)
                     tgatgaAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTgg                                                                                                                               >  1:2531553/1‑148 (MQ=255)
                      gatgaAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGc                                                                                                                              >  1:2124558/1‑148 (MQ=255)
                       atgaAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCg                                                                                                                             >  2:3403498/1‑148 (MQ=255)
                         gaAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGaa                                                                                                                           >  2:2420884/1‑148 (MQ=255)
                          aaaTCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATTCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATTGTTTGGCCTTCGTGGCGAAc                                                                                                                          <  2:2350206/148‑1 (MQ=255)
                            aTCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACAt                                                                                                                        >  1:803160/1‑148 (MQ=255)
                              cTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCTGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGc                                                                                                                      >  2:1738740/1‑148 (MQ=255)
                              cTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGc                                                                                                                      >  2:2615053/1‑148 (MQ=255)
                                ttCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAg                                                                                                                    <  1:962192/148‑1 (MQ=255)
                                    tAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCTATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTcc                                                                                                                <  2:1389820/148‑1 (MQ=255)
                                    tAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTcc                                                                                                                >  2:1547212/1‑148 (MQ=255)
                                       ggATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCt                                                                                                             >  1:2592056/1‑148 (MQ=255)
                                        gATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCtt                                                                                                            <  2:676612/148‑1 (MQ=255)
                                        gATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCtt                                                                                                            >  2:2936395/1‑148 (MQ=255)
                                          ttGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTGcc                                                                                                          >  2:3178471/1‑148 (MQ=255)
                                              cAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCa                                                                                                      <  2:2257907/148‑1 (MQ=255)
                                               aGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCAc                                                                                                     <  2:2111004/148‑1 (MQ=255)
                                               aGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCAc                                                                                                     <  2:1628420/148‑1 (MQ=255)
                                               aGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCAc                                                                                                     <  2:1628140/148‑1 (MQ=255)
                                                 ttAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCa                                                                                                   >  1:445530/1‑148 (MQ=255)
                                                 ttAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCa                                                                                                   <  2:2654617/148‑1 (MQ=255)
                                                 ttAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCa                                                                                                   >  2:1149390/1‑148 (MQ=255)
                                                  tAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCAt                                                                                                  >  2:758340/1‑148 (MQ=255)
                                                          cAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGAttt                                                                                          <  2:481905/148‑1 (MQ=255)
                                                             gCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTtcgt                                                                                       >  2:1894045/1‑148 (MQ=255)
                                                              cGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTtcgtc                                                                                      >  2:1069993/1‑148 (MQ=255)
                                                               gACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTtcgtcg                                                                                     <  1:1126888/148‑1 (MQ=255)
                                                               gACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTtcgtcg                                                                                     <  2:1141293/148‑1 (MQ=255)
                                                                aCATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTcgtcgt                                                                                    <  1:2274804/148‑1 (MQ=255)
                                                                aCATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTcgtcgt                                                                                    <  1:2258038/148‑1 (MQ=255)
                                                                aCATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTcgtcgt                                                                                    >  1:3160440/1‑148 (MQ=255)
                                                                 cATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTa                                                                                   >  1:3146494/1‑148 (MQ=255)
                                                                   tACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGt                                                                                 <  2:3512262/148‑1 (MQ=255)
                                                                   tACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGt                                                                                 <  2:421398/148‑1 (MQ=255)
                                                                    aCAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCCGCACCATGTCGATTTCGTCGTAGtt                                                                                <  1:1482282/148‑1 (MQ=255)
                                                                    aCAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGtt                                                                                <  1:54189/148‑1 (MQ=255)
                                                                     cAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTc                                                                               <  1:1355055/148‑1 (MQ=255)
                                                                       ggAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAg                                                                             >  1:723902/1‑148 (MQ=255)
                                                                        gAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCTATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAgg                                                                            >  2:2799509/1‑148 (MQ=255)
                                                                        gAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAgg                                                                            <  2:1479832/148‑1 (MQ=255)
                                                                        gAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAgg                                                                            <  2:1481113/148‑1 (MQ=255)
                                                                          aTTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGt                                                                          <  2:446098/148‑1 (MQ=255)
                                                                          aTTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGt                                                                          <  1:2913507/148‑1 (MQ=255)
                                                                          aTTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGt                                                                          <  1:1054706/148‑1 (MQ=255)
                                                                                   tGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGgaa                                                                 >  1:346069/1‑148 (MQ=255)
                                                                                   tGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGgaa                                                                 <  2:2932212/148‑1 (MQ=255)
                                                                                      aTGTCGTACCAAAGTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGgaagaa                                                              <  2:459743/148‑1 (MQ=255)
                                                                                          cGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCg                                                          >  1:367728/1‑148 (MQ=255)
                                                                                               cAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTtctc                                                     >  1:1889047/1‑148 (MQ=255)
                                                                                                aaaTTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTcc                                                    >  2:418066/1‑148 (MQ=255)
                                                                                                aaaTTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTcc                                                    >  2:417739/1‑148 (MQ=255)
                                                                                                 aaTTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTTGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCACGGTCCACCGGAAGAAAGCGTTCTccc                                                   >  2:1239996/1‑148 (MQ=255)
                                                                                                  aTTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCa                                                  <  2:3227267/148‑1 (MQ=255)
                                                                                                   ttCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCAc                                                 >  1:1038955/1‑148 (MQ=255)
                                                                                                   ttCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCAc                                                 <  1:1630379/148‑1 (MQ=255)
                                                                                                   ttCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCAc                                                 >  2:2022491/1‑148 (MQ=255)
                                                                                                   ttCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCAc                                                 >  1:1038951/1‑148 (MQ=255)
                                                                                                         gCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATa                                           >  2:1402467/1‑148 (MQ=255)
                                                                                                         gCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATa                                           <  1:2333769/148‑1 (MQ=255)
                                                                                                            ggAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATaaaa                                        <  2:1290092/148‑1 (MQ=255)
                                                                                                             gAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCGTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAAc                                       <  1:3296100/148‑1 (MQ=255)
                                                                                                              aGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAAcc                                      >  2:2992609/1‑148 (MQ=255)
                                                                                                              aGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAAcc                                      >  2:2992676/1‑148 (MQ=255)
                                                                                                               gCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACcg                                     >  1:1048078/1‑148 (MQ=255)
                                                                                                                ccTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACcgg                                    >  1:2875999/1‑148 (MQ=255)
                                                                                                                  tttCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACcggcg                                  >  2:2184222/1‑148 (MQ=255)
                                                                                                                        gATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGTGTTCTCCCACTGCATAAAACCGGCGGCCAtg                            <  2:1369613/148‑1 (MQ=255)
                                                                                                                        gATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCAtg                            <  2:2492675/148‑1 (MQ=255)
                                                                                                                          tAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCAtgct                          >  1:3432490/1‑148 (MQ=255)
                                                                                                                          tAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCAtgct                          >  2:1175214/1‑148 (MQ=255)
                                                                                                                          tAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCAtgct                          >  1:2649261/1‑148 (MQ=255)
                                                                                                                           aaaCATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCAtgctg                         <  2:80693/148‑1 (MQ=255)
                                                                                                                            aaCATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCAtgctgc                        <  1:1983682/148‑1 (MQ=255)
                                                                                                                            aaCATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCAtgctgc                        >  2:728880/1‑148 (MQ=255)
                                                                                                                             aCATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCAtgctgct                       >  1:3202648/1‑148 (MQ=255)
                                                                                                                              cATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCAtgctgctg                      <  1:1548209/148‑1 (MQ=255)
                                                                                                                                tCACGTCGCCTGCTTTGGCTATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGcc                    >  1:487390/1‑148 (MQ=255)
                                                                                                                                 cACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCGTCCAGCACCATGGCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCcg                   <  2:946414/148‑1 (MQ=255)
                                                                                                                                 cACGTCGCCTGCTTGGGCGATCATGGTTTGGCCTTGGTGGCGAACATGCAGCTCCCCTTCCAGCACCTTGTCGATTTCGTCGTAGTTCAGGGTCCACGGGATGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCcg                   <  1:1239996/148‑1 (MQ=255)
                                                                                                                                      cGCCTGCTTTGGCTATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCgtcgtc              >  1:3183334/1‑148 (MQ=255)
                                                                                                                                        ccTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGtctc            >  1:2407955/1‑148 (MQ=255)
                                                                                                                                                  cGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCACAAg  >  2:2871369/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCGCTCTTAGCCATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGACATACAGGAATTTCACGCTGGATGTCGTACCAAATTCGATGCTGGAGCCTTTCGGGATAAACATCACGTCGCCTGCTTTGGCGATCATGGTTTGGCCTTCGTGGCGAACATGCAGCTCCCCTTCCAGCACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCACAAG  >  CP009273/2567628‑2567921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑