Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,579,561 G→T 100% G158C (GGC→TGC)  narQ → sensory histidine kinase in two‑component regulatory system with NarP (NarL)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,579,5610GT100.0% 588.4 / NA 176G158C (GGC→TGC) narQsensory histidine kinase in two‑component regulatory system with NarP (NarL)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (94/82);  total (94/82)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 185 total aligned reads displayed.

GAATAATCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCGGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAACCTGCCGA  >  CP009273/2579416‑2579705
                                                                                                                                                 |                                                                                                                                                
  ataatCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCAt                                                                                                                                              >  1:1403281/1‑148 (MQ=255)
   taatCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATc                                                                                                                                             <  1:539154/148‑1 (MQ=255)
   taatCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATc                                                                                                                                             >  1:2986287/1‑148 (MQ=255)
    aatCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCg                                                                                                                                            >  2:2167331/1‑148 (MQ=255)
     atCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCgg                                                                                                                                           >  2:2780882/1‑148 (MQ=255)
      tCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGt                                                                                                                                          <  1:1826961/148‑1 (MQ=255)
        ggCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTAt                                                                                                                                        <  2:2910640/148‑1 (MQ=255)
        ggCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTAt                                                                                                                                        <  2:2878686/148‑1 (MQ=255)
            cAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTc                                                                                                                                    <  1:2138913/148‑1 (MQ=255)
             aGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCa                                                                                                                                   <  1:717355/148‑1 (MQ=255)
             aGCAACGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCa                                                                                                                                   <  2:3452347/148‑1 (MQ=255)
               cAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACg                                                                                                                                 >  2:2913984/1‑148 (MQ=255)
                aaGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGc                                                                                                                                <  2:2661177/148‑1 (MQ=255)
                   ggCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTgg                                                                                                                             >  2:1547269/1‑148 (MQ=255)
                    gCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGt                                                                                                                            >  1:2850722/1‑148 (MQ=255)
                    gCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGt                                                                                                                            >  1:2198690/1‑148 (MQ=255)
                     cGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTc                                                                                                                           >  1:692323/1‑148 (MQ=255)
                     cGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTc                                                                                                                           <  2:892169/148‑1 (MQ=255)
                           gCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCtttttt                                                                                                                     <  1:896666/148‑1 (MQ=255)
                             cGTGGTCTCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTAc                                                                                                                   <  2:2190646/148‑1 (MQ=255)
                             cGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTAc                                                                                                                   >  2:2440229/1‑148 (MQ=255)
                               tGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACtt                                                                                                                 >  2:2189510/1‑148 (MQ=255)
                                 gTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTg                                                                                                               >  1:593782/1‑148 (MQ=255)
                                    tCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTgcgc                                                                                                            <  1:2977032/148‑1 (MQ=255)
                                        gCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTTCTCCGCa                                                                                                        >  2:3061278/1‑148 (MQ=255)
                                        gCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCa                                                                                                        >  1:1884680/1‑148 (MQ=255)
                                         ccAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCAt                                                                                                       <  2:2420576/148‑1 (MQ=255)
                                         ccAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCAt                                                                                                       <  2:2306859/148‑1 (MQ=255)
                                         ccAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCAt                                                                                                       <  1:1528996/148‑1 (MQ=255)
                                         ccAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCAt                                                                                                       <  2:3106059/148‑1 (MQ=255)
                                           aaTATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATAc                                                                                                     >  1:2339633/1‑148 (MQ=255)
                                            atatTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACg                                                                                                    >  2:126774/1‑148 (MQ=255)
                                              atTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGcc                                                                                                  >  2:823630/1‑148 (MQ=255)
                                               ttAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCa                                                                                                 <  1:2651658/148‑1 (MQ=255)
                                                taataaTTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCAt                                                                                                <  1:2872536/148‑1 (MQ=255)
                                                taataaTTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCAt                                                                                                >  2:1143086/1‑148 (MQ=255)
                                                taataaTTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCAt                                                                                                >  2:2744496/1‑148 (MQ=255)
                                                taataaTTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCAt                                                                                                >  1:1670616/1‑148 (MQ=255)
                                                taataaTTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCAt                                                                                                >  2:2343254/1‑148 (MQ=255)
                                                  ataatTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCa                                                                                              >  1:1549483/1‑148 (MQ=255)
                                                     atTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAggt                                                                                           >  1:1059591/1‑148 (MQ=255)
                                                      ttATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAggtg                                                                                          <  1:652685/148‑1 (MQ=255)
                                                      ttATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAggtg                                                                                          >  1:793481/1‑148 (MQ=255)
                                                       tATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAggtgg                                                                                         <  2:2875231/148‑1 (MQ=255)
                                                       tATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAggtgg                                                                                         >  2:1420083/1‑148 (MQ=255)
                                                          gTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTg                                                                                      >  2:193523/1‑148 (MQ=255)
                                                          gTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTg                                                                                      >  2:578110/1‑148 (MQ=255)
                                                           ttAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGc                                                                                     <  1:3465889/148‑1 (MQ=255)
                                                           ttAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGc                                                                                     >  2:2501786/1‑148 (MQ=255)
                                                              aTCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGcccc                                                                                  >  2:122944/1‑148 (MQ=255)
                                                               tCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCg                                                                                 >  2:798621/1‑148 (MQ=255)
                                                                cAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGc                                                                                >  2:3447859/1‑148 (MQ=255)
                                                                cAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGc                                                                                >  2:1105923/1‑148 (MQ=255)
                                                                     aGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAAt                                                                           >  2:3368452/1‑148 (MQ=255)
                                                                     aGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAAt                                                                           >  1:1082092/1‑148 (MQ=255)
                                                                     aGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAAt                                                                           <  2:1127386/148‑1 (MQ=255)
                                                                      gACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATc                                                                          <  2:1234111/148‑1 (MQ=255)
                                                                      gACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATc                                                                          >  1:1072443/1‑148 (MQ=255)
                                                                        ccTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAg                                                                        >  1:3234337/1‑148 (MQ=255)
                                                                        ccTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAg                                                                        >  2:1021238/1‑148 (MQ=255)
                                                                         cTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGc                                                                       >  2:1331926/1‑148 (MQ=255)
                                                                         cTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGc                                                                       <  1:723290/148‑1 (MQ=255)
                                                                          tGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCt                                                                      <  1:2403151/148‑1 (MQ=255)
                                                                           gTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTg                                                                     >  1:3038048/1‑148 (MQ=255)
                                                                            ttCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTgg                                                                    >  1:1137643/1‑148 (MQ=255)
                                                                             tCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGt                                                                   <  1:3412550/148‑1 (MQ=255)
                                                                                 aCTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTAcc                                                               >  1:3120034/1‑148 (MQ=255)
                                                                                   tGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGc                                                             >  1:103467/1‑148 (MQ=255)
                                                                                      cTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAg                                                          >  1:2511357/1‑148 (MQ=255)
                                                                                       tttACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGt                                                         <  2:364994/148‑1 (MQ=255)
                                                                                       tttACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGt                                                         <  2:364854/148‑1 (MQ=255)
                                                                                        ttACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTc                                                        >  1:2634854/1‑148 (MQ=255)
                                                                                        ttACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTc                                                        <  1:9479/148‑1 (MQ=255)
                                                                                         tACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCa                                                       >  2:418463/1‑148 (MQ=255)
                                                                                           cAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGc                                                     >  1:1578478/1‑148 (MQ=255)
                                                                                                     cTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAaca                                           <  2:1778697/148‑1 (MQ=255)
                                                                                                        aaCGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACgg                                        <  1:2315791/148‑1 (MQ=255)
                                                                                                        aaCGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACgg                                        <  1:1386197/148‑1 (MQ=255)
                                                                                                            cAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAg                                    >  2:2703835/1‑148 (MQ=255)
                                                                                                             aaaaTGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGt                                   <  2:458282/148‑1 (MQ=255)
                                                                                                             aaaaTGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGt                                   <  1:393838/148‑1 (MQ=255)
                                                                                                                aTGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCg                                <  2:628516/148‑1 (MQ=255)
                                                                                                                 tgctgcTGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGa                               <  2:2608630/148‑1 (MQ=255)
                                                                                                                  gctgctGGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGAc                              >  1:205747/1‑148 (MQ=255)
                                                                                                                     gctgGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTcg                           <  1:2444336/148‑1 (MQ=255)
                                                                                                                     gctgGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTcg                           <  1:1972432/148‑1 (MQ=255)
                                                                                                                     gctgGTGGTGGCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTcg                           <  1:591433/148‑1 (MQ=255)
                                                                                                                           ggtggCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTcgccgccg                     <  2:2171728/148‑1 (MQ=255)
                                                                                                                           ggtggCGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTcgccgccg                     <  1:3447859/148‑1 (MQ=255)
                                                                                                                                cGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGa                <  2:2850722/148‑1 (MQ=255)
                                                                                                                                cGATTTCCCTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGa                <  2:2198690/148‑1 (MQ=255)
                                                                                                                                       ccTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAAc         <  1:1227895/148‑1 (MQ=255)
                                                                                                                                       ccTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAAc         >  2:3325835/1‑148 (MQ=255)
                                                                                                                                       ccTGGCTGGCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATAAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAAc         >  1:2737872/1‑148 (MQ=255)
                                                                                                                                        ctggctggCTGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAAcc        <  1:2343254/148‑1 (MQ=255)
                                                                                                                                           gctggctgCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAACCTGc     >  2:3207064/1‑148 (MQ=255)
                                                                                                                                           gctggctgCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAACCTGc     >  2:1744129/1‑148 (MQ=255)
                                                                                                                                           gctggctgCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAACCTGc     >  1:3117963/1‑148 (MQ=255)
                                                                                                                                           gctggctgCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAACCTGc     <  1:3428720/148‑1 (MQ=255)
                                                                                                                                              ggctgcATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAACCTGCCGa  >  1:1825693/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GAATAATCGGCTCAGCAAGGGCGATTTGCCGTGGTATCAGGCCAATATTAATAATTATGTTAATCAGATAGACCTGTTCGTACTGGCTTTACAGCACTACGCTGAACGCAAAATGCTGCTGGTGGTGGCGATTTCCCTGGCTGGCGGCATCGGTATTTTCACGCTGGTCTTTTTTACTTTGCGCCGCATACGCCATCAGGTGGTTGCCCCGCTGAATCAGCTGGTTACCGCCAGTCAGCGTATTGAACACGGGCAGTTCGACTCGCCGCCGCTGGATACCAACCTGCCGA  >  CP009273/2579416‑2579705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑