Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,598,312 C→A 9.1% A48E (GCG→GAG)  hyfD → hydrogenase 4, membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,598,3120CA 9.1% 603.0 / 24.3 176A48E (GCG→GAG) hyfDhydrogenase 4, membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (81/78);  new base A (6/11);  total (87/89)
Fisher's exact test for biased strand distribution p-value = 3.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 183 total aligned reads displayed.

GAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTTGTGGTGGTGTTTCTCGGTTTGCTGGTCACGATCTACTCCA  >  CP009273/2598166‑2598458
                                                                                                                                                  |                                                                                                                                                  
gAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCcgcg                                                                                                                                                   <  1:666919/148‑1 (MQ=255)
gAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCcgcg                                                                                                                                                   <  1:667047/148‑1 (MQ=255)
gAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCcgcg                                                                                                                                                   <  1:776211/148‑1 (MQ=255)
 aaTATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGt                                                                                                                                                  <  2:2542981/148‑1 (MQ=255)
   tatGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTGGTTTTAGCCACAACGTCGGGCCGCCGAATTGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTACGAGttt                                                                                                                                                >  1:3541077/1‑148 (MQ=255)
    atGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGAGtttt                                                                                                                                               <  2:1460346/148‑1 (MQ=255)
    atGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGAGtttt                                                                                                                                               <  2:1921255/148‑1 (MQ=255)
    atGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTAGCCACAACGACGGGCCGCCGAATGGGGGGTTTTTTTGGCCGCGCTGACCACCCTGTGCATGTTGTCACTGATCTCCGCGgttt                                                                                                                                               >  1:1629230/1‑148 (MQ=255)
     tGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTa                                                                                                                                              >  2:859754/1‑148 (MQ=255)
     tGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTa                                                                                                                                              <  1:977341/148‑1 (MQ=255)
     tGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTGTGGGTTTGGCCACAACCTAGGGGCGCCGACTGGGGGGGTTTTTTAGCCCCGCTGACCACGCGGTGCCTTTTTTGACTGATATCCCCGgtttt                                                                                                                                              >  1:3307385/1‑147 (MQ=255)
      ggAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTGTGGTTTTAGCCCCAACCTCGGGCCGCCGCCTGGGGGGTTTTTTTTGCCGCGCTGACCACCCCGTGCCAGGTGTCACTGATATACCCGtttttt                                                                                                                                             >  1:645094/1‑146 (MQ=255)
       gAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATc                                                                                                                                            >  2:2924250/1‑148 (MQ=255)
       gAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACCTCGGGCCGCCGAATGGGGGGTTTTGTTAGCCGCGCTGACCACCCTGTGCATGTTGTCACTGATATCCGCGTTTTTTc                                                                                                                                            >  1:1975607/1‑148 (MQ=255)
       gAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTGTGGGTTTAGCCACAACCTCGGGCCGCCCCCAGGGGGGGTTTGTTGGCCGCGCTGACCACCCTGTGCATGTTGTCACTGATATCCGCGGTTTATc                                                                                                                                            >  1:1934441/1‑148 (MQ=255)
        aaaaTCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTAGCCACAACGTCGGGCCGCCGAAAGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCa                                                                                                                                           >  1:2061288/1‑148 (MQ=255)
        aaaaTCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTGTCGTTTTAGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTAtaa                                                                                                                                           >  1:289585/1‑146 (MQ=255)
         aaaTCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAg                                                                                                                                          <  1:3292791/148‑1 (MQ=255)
         aaaTCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAg                                                                                                                                          <  1:1527501/148‑1 (MQ=255)
         aaaTCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAg                                                                                                                                          <  1:1527500/148‑1 (MQ=255)
             cTTGCTCTGACGACGTTAGTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCg                                                                                                                                      <  2:2162614/148‑1 (MQ=255)
               tGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACCTCGGGCCGCCGAAGGGGGGGTTTTTTTAGCCGCGCTGACCACGCTGTGCATGTTTTGACAGATCTCCGCGTTTTATCAGGCCGAt                                                                                                                                    >  1:2288296/1‑148 (MQ=255)
                   ctGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAg                                                                                                                                >  2:634366/1‑148 (MQ=255)
                   ctGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTAGCCACAACCTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCAGACCACGCAGTGCATGTTGTCACTGATATCCGCGTTTTATAAGGCCGATAAAg                                                                                                                                >  2:634439/1‑148 (MQ=255)
                      acgacgTTATTGCTGCCTTTTATCGGCGCACTGGTCGTGTGGTTTTAGCCACAACCTCGGGCCGCCGGCAGGGGGGTTTTTTTGGCCCCGCAGACCACCCGGTGCATGTTGTAACAGATATCCGCGGTTTATAAGGCCGATAAAAttt                                                                                                                             >  1:1069704/1‑147 (MQ=255)
                       cgacgTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTAGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGAGTTTTATCACGCCGATAAAGTTGc                                                                                                                            >  2:586593/1‑148 (MQ=255)
                         acgTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGCATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCCCTGATCTCCGCGGTTTATCAGGCCGATAAAGTTGCCg                                                                                                                          >  2:179584/1‑148 (MQ=255)
                         acgTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACAGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCg                                                                                                                          >  2:292291/1‑148 (MQ=255)
                              aTTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCacg                                                                                                                     <  2:3333464/148‑1 (MQ=255)
                              aTTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCacg                                                                                                                     <  2:659469/148‑1 (MQ=255)
                              aTTGCTGCCTTTTATCGGCGCACTGGTCGTGTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCacg                                                                                                                     <  2:3333459/148‑1 (MQ=255)
                                tgctgcCTTTTATCGGCGCACTGGTCGTGTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCacgtt                                                                                                                   >  2:3159380/1‑148 (MQ=255)
                                     ccTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCacgttgacgt                                                                                                              <  1:3417431/148‑1 (MQ=255)
                                     ccTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGGTTTATCAGGCCGATAAAGTTGCCGTCacgttgacgg                                                                                                              >  2:1787621/1‑147 (MQ=255)
                                       ttttATCGGCGCACTGGTCGTTTCGTTTTAGCCACAACCTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACAGATCTCCGCGGTTTATAAGGCCGATAAAGTTGCCGGCacgttgacgttg                                                                                                            >  2:1633541/1‑148 (MQ=255)
                                        tttATCGGCGCACTGGTCGTTTCGTTTTCGCCACAAAGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTgg                                                                                                           >  2:877263/1‑148 (MQ=255)
                                         ttATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGt                                                                                                          <  1:1132839/148‑1 (MQ=255)
                                            tCGGCGCACTGGTCGTGTCGTTTTCGCCACCAACTCGGGCCGCCGCATGGGGGGGTTTTTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCCTTTTATCAGGCCGATAAAGTTGCCGTCACGGTGACGTTGGTCaa                                                                                                       >  2:1581498/1‑148 (MQ=255)
                                                cgcACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTTTTAGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATAAGGCCGATAAATTTGCCGTCAAGTTTTCGTTGGTAAACGTg                                                                                                   >  2:1839150/1‑148 (MQ=255)
                                                  cACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTggg                                                                                                 <  1:449628/148‑1 (MQ=255)
                                                       gTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGAtg                                                                                            <  2:2288317/148‑1 (MQ=255)
                                                         cgtttcgtttTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGAtgtg                                                                                          <  2:2204556/148‑1 (MQ=255)
                                                         cgtttcgtttTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTCGTCAACGTGGGGGAtgtg                                                                                          <  2:2201101/148‑1 (MQ=255)
                                                         cgtttcgtttTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGAGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGAtgtg                                                                                          >  2:21592/1‑148 (MQ=255)
                                                         cgtttcgtgtTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGAtgtg                                                                                          <  2:2204557/148‑1 (MQ=255)
                                                             tcgtttTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTAACGTTGACGTTGGTCAACGTGGGGGATGTGGCgt                                                                                      <  1:2131687/148‑1 (MQ=255)
                                                                 tttCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCgttgtt                                                                                  >  2:2775432/1‑148 (MQ=255)
                                                                 tttCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCgttgtt                                                                                  >  1:1168668/1‑148 (MQ=255)
                                                                  ttCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGttt                                                                                 <  1:3480749/148‑1 (MQ=255)
                                                                      ccACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGcc                                                                             <  2:3472396/148‑1 (MQ=255)
                                                                        acaACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTg                                                                           >  2:2028273/1‑148 (MQ=255)
                                                                          aaCGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGGTGTTTGGCCTGGt                                                                         >  1:2866672/1‑148 (MQ=255)
                                                                           aCGTCGGGCCGCCGAATGGGGGTTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTc                                                                        <  1:959124/148‑1 (MQ=255)
                                                                           aCGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTc                                                                        <  1:1683660/148‑1 (MQ=255)
                                                                            cGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCa                                                                       >  1:2818814/1‑148 (MQ=255)
                                                                             gTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCAt                                                                      <  1:1715342/148‑1 (MQ=255)
                                                                             gTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCAt                                                                      <  2:1691434/148‑1 (MQ=255)
                                                                               cGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTg                                                                    <  1:2544545/148‑1 (MQ=255)
                                                                               cGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTg                                                                    <  1:3283789/148‑1 (MQ=255)
                                                                                gggCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGa                                                                   <  2:131243/148‑1 (MQ=255)
                                                                                gggCCGCCGAATGGGGGGTTTTGGTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGa                                                                   <  2:3098590/148‑1 (MQ=255)
                                                                                  gccgccGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATc                                                                 >  1:876429/1‑148 (MQ=255)
                                                                                  gccgccGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATc                                                                 >  1:2880333/1‑148 (MQ=255)
                                                                                     gccgAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGAGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATcgcg                                                              <  1:606728/148‑1 (MQ=255)
                                                                                        gAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTAAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGa                                                           <  1:797348/148‑1 (MQ=255)
                                                                                         aagggggggTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAg                                                          >  2:1045687/4‑148 (MQ=255)
                                                                                            ggggggTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATAGCGTGAGTAc                                                       >  1:1058480/1‑148 (MQ=255)
                                                                                              ggggTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGc                                                     >  2:1322755/1‑148 (MQ=255)
                                                                                              ggggTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGc                                                     >  2:3316619/1‑148 (MQ=255)
                                                                                              ggggTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGc                                                     <  2:2596455/148‑1 (MQ=255)
                                                                                                 gTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGa                                                  <  2:43943/148‑1 (MQ=255)
                                                                                                  ttttGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGAt                                                 <  1:2924250/148‑1 (MQ=255)
                                                                                                  ttttGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGAt                                                 >  1:2552747/1‑148 (MQ=255)
                                                                                                   tttGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGAGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGAtt                                                <  1:2810949/148‑1 (MQ=255)
                                                                                                    ttGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTc                                               <  2:549590/148‑1 (MQ=255)
                                                                                                        tCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGtt                                           <  1:3045122/148‑1 (MQ=255)
                                                                                                          gccgcGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTtg                                         <  2:1103567/148‑1 (MQ=255)
                                                                                                             gcgcTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTTgtgg                                      <  2:1975607/148‑1 (MQ=255)
                                                                                                              cgcTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTTgtggt                                     >  1:534307/1‑148 (MQ=255)
                                                                                                                cTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTTgtggtgg                                   <  2:1504317/148‑1 (MQ=255)
                                                                                                                cTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTTgtggtgg                                   <  2:1210283/148‑1 (MQ=255)
                                                                                                                     cACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTTGTGGTGGTGttt                              >  1:3471464/1‑148 (MQ=255)
                                                                                                                      aCGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTTGTGGTGGTGTTtc                             <  2:1542856/148‑1 (MQ=255)
                                                                                                                       cGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTTGTGGTGGTGTTtct                            >  1:2340835/1‑148 (MQ=255)
                                                                                                                         cTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTTGTGGTGGTGTTTCTCg                          >  2:1377227/1‑148 (MQ=255)
                                                                                                                           gtgCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTTGTGGTGGTGTTTCTCGGt                        <  2:2031565/148‑1 (MQ=255)
                                                                                                                           gtgCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTTGTGGTGGTGTTTCTCGGt                        <  2:1257472/148‑1 (MQ=255)
                                                                                                                             gCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTTGTGGTGGTGTTTCTCGGttt                      <  1:383187/148‑1 (MQ=255)
                                                                                                                              cATGTTGTCACTGATATCCGCTTATTATCAGGCAGAAAAAGTTGCCGTAAATTAGAATTTGGAAAACCTGGGTGATGTGGCGTAGATTGGACTTGGAATTGATAGCGTTAGTACGCTGATTCTGTTTGTGGTGTTGTTTCTCGGtttt                     >  2:203361/1‑147 (MQ=255)
                                                                                                                                 gttgtCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACTTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGAGTCTGTTTGTGGTGGTGTTTCTCGGTTTGCTg                  >  2:1744700/1‑148 (MQ=255)
                                                                                                                                        cTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTTGTGGTGGTGTTTCTCGGTTTGCTGGTCACGa           >  1:3261729/1‑148 (MQ=255)
                                                                                                                                        cTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTTGTGGTGGTGTTTCTCGGTTTGCTGGTCACGa           >  2:1579322/1‑148 (MQ=255)
                                                                                                                                         tGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTTGTGGTGGTGTTTCTCGGTTTGCTGGTCACGAt          >  1:2632660/1‑148 (MQ=255)
                                                                                                                                           aTCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTTGTGGTGGTGTTTCTCGGTTTGCTGGTCACGATCt        <  1:962717/148‑1 (MQ=255)
                                                                                                                                           aTCTCCGAGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTTGTGGTGGTGTTTCTCGGTTTGCTGGTCACGATCt        >  1:352201/1‑148 (MQ=255)
                                                                                                                                            tctcCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTTGTGGTGGTGTTTCTCGGTTTGCTGGTCACGATCTa       <  2:3223720/148‑1 (MQ=255)
                                                                                                                                              tcCGAGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTTGTGGTGGTGTTTCTCGGTTTGCTGGTCACGATCTACt     <  1:586593/148‑1 (MQ=255)
                                                                                                                                               ccGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGCTGTTGGGGTGTGTGGCCTGGGCCTTGATCGCGTGAGTACGCGGGTTCTGTGTGTGGTGGGGTTTGTCGGTTTGCTGGGCCCGATCTaatg    >  1:1284114/1‑145 (MQ=255)
                                                                                                                                                 gcgTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTTGTGGTGGTGTTTCTCGGTTTGCTGGTCACGATCTACTCCa  >  1:1764516/1‑148 (MQ=255)
                                                                                                                                                 gcgTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTTGTGGTGGTGTTTCTCGGTTTGCTGGTCACGATCTACTCCa  <  2:645094/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTTGTGGTGGTGTTTCTCGGTTTGCTGGTCACGATCTACTCCA  >  CP009273/2598166‑2598458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑