Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,659,594 C→A 100% L267L (CTC→CTA)  csiE → stationary phase inducible protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,659,5940CA97.7% 676.8 / 5.8 217L267L (CTC→CTA) csiEstationary phase inducible protein
Reads supporting (aligned to +/- strand):  ref base C (0/5);  new base A (117/95);  total (117/100)
Fisher's exact test for biased strand distribution p-value = 1.97e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 217 total aligned reads displayed.

GGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCT  >  CP009273/2659449‑2659738
                                                                                                                                                 |                                                                                                                                                
 ggTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGcc                                                                                                                                               <  2:2216150/148‑1 (MQ=255)
 ggTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGcc                                                                                                                                               <  2:426620/148‑1 (MQ=255)
   ttCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCa                                                                                                                                             <  2:1454182/148‑1 (MQ=255)
    tCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAg                                                                                                                                            >  1:1173513/1‑148 (MQ=255)
    tCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAg                                                                                                                                            <  1:1050413/148‑1 (MQ=255)
          tCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTa                                                                                                                                      >  1:782283/1‑148 (MQ=255)
          tCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTa                                                                                                                                      >  2:3217901/1‑148 (MQ=255)
          tCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTa                                                                                                                                      >  2:1248547/1‑148 (MQ=255)
           ccATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTaa                                                                                                                                     >  1:2753766/1‑148 (MQ=255)
           ccATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTaa                                                                                                                                     <  1:1322088/148‑1 (MQ=255)
            cATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTaaa                                                                                                                                    <  1:1567931/148‑1 (MQ=255)
              ttGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATc                                                                                                                                  >  2:1888522/1‑148 (MQ=255)
               tGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCg                                                                                                                                 >  1:2201764/1‑148 (MQ=255)
                 cGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCa                                                                                                                               >  1:19239/1‑148 (MQ=255)
                   ggATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAgt                                                                                                                             >  2:3423085/1‑148 (MQ=255)
                      tGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAgtttg                                                                                                                          <  2:2837934/148‑1 (MQ=255)
                       gcgcATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAgtttgt                                                                                                                         <  1:2622914/148‑1 (MQ=255)
                       gcgcATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAgtttgt                                                                                                                         <  1:3333066/148‑1 (MQ=255)
                          cATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGtttgtttg                                                                                                                      <  2:1082790/148‑1 (MQ=255)
                          cATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGtttgtttg                                                                                                                      <  1:2573498/148‑1 (MQ=255)
                            tCAGCGGGACAGACAATTGCGCCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGcc                                                                                                                    <  1:2972981/148‑1 (MQ=255)
                             cAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCa                                                                                                                   <  1:2258837/148‑1 (MQ=255)
                              aGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCAt                                                                                                                  <  2:710921/148‑1 (MQ=255)
                              aGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCAt                                                                                                                  <  2:1815001/148‑1 (MQ=255)
                              aGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCAt                                                                                                                  <  2:3123609/148‑1 (MQ=255)
                               gCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATc                                                                                                                 >  2:2414846/1‑148 (MQ=255)
                               gCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTAGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATc                                                                                                                 >  2:3273949/1‑148 (MQ=255)
                               gCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTAGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATc                                                                                                                 >  2:1641614/1‑148 (MQ=255)
                                  ggACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGt                                                                                                              >  2:1579451/1‑148 (MQ=255)
                                  ggACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGt                                                                                                              >  2:2007230/1‑148 (MQ=255)
                                  ggACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGt                                                                                                              <  1:3246803/148‑1 (MQ=255)
                                  ggACAGACAAGTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGt                                                                                                              <  1:1222138/148‑1 (MQ=255)
                                    acagacaATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTAt                                                                                                            >  2:1054951/1‑148 (MQ=255)
                                      agacaATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTg                                                                                                          <  1:1144097/148‑1 (MQ=255)
                                      agacaATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTg                                                                                                          >  2:2493155/1‑148 (MQ=255)
                                      agacaATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTg                                                                                                          <  1:238330/148‑1 (MQ=255)
                                       gacaATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGa                                                                                                         <  2:2598708/148‑1 (MQ=255)
                                          aagtGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGataa                                                                                                      <  2:2861389/145‑1 (MQ=255)
                                          aaTTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGataa                                                                                                      <  1:336715/148‑1 (MQ=255)
                                           atggCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGataat                                                                                                     <  2:2160184/145‑1 (MQ=255)
                                            ttGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGataata                                                                                                    <  2:2714337/148‑1 (MQ=255)
                                             tGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAgtttgt                                                                                                                         >  1:2009295/1‑126 (MQ=255)
                                               cGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATAccc                                                                                                 <  2:3062916/148‑1 (MQ=255)
                                                 tCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTg                                                                                               <  1:2245900/148‑1 (MQ=255)
                                                 tCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTg                                                                                               >  1:877353/1‑148 (MQ=255)
                                                    gTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTACCg                                                                                            >  2:2234634/1‑148 (MQ=255)
                                                     tCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCgg                                                                                           >  1:2611710/1‑148 (MQ=255)
                                                            aaCGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGtt                                                                                    >  2:601753/1‑148 (MQ=255)
                                                            aaCGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGtt                                                                                    <  1:1237145/148‑1 (MQ=255)
                                                               gTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGc                                                                                 <  2:1093418/148‑1 (MQ=255)
                                                                tCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGcc                                                                                >  1:3451287/1‑148 (MQ=255)
                                                                tCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGcc                                                                                >  1:3451006/1‑148 (MQ=255)
                                                                   ggTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTCTTGATAATACCCTGCCGGAAGAGTTCGCCAGa                                                                             >  1:943813/1‑148 (MQ=255)
                                                                   ggTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGa                                                                             >  1:943670/1‑148 (MQ=255)
                                                                   ggTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGa                                                                             >  1:671815/1‑148 (MQ=255)
                                                                        aCCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTa                                                                        >  2:709245/1‑148 (MQ=255)
                                                                        aCCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTa                                                                        >  1:3488242/1‑148 (MQ=255)
                                                                          cATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTAcc                                                                      <  2:1379924/148‑1 (MQ=255)
                                                                            ttttCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCa                                                                    >  2:435204/1‑148 (MQ=255)
                                                                            ttttCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCa                                                                    <  2:1917505/148‑1 (MQ=255)
                                                                             tttCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCAc                                                                   >  1:939081/1‑148 (MQ=255)
                                                                                         ggAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGca                                                       <  2:827805/148‑1 (MQ=255)
                                                                                          gAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGcac                                                      >  1:2003319/1‑148 (MQ=255)
                                                                                            aaTGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGcacca                                                    >  1:2977017/1‑148 (MQ=255)
                                                                                            aaTGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGcacca                                                    >  1:3276545/1‑148 (MQ=255)
                                                                                                 tCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCcgc                                               >  2:1691194/1‑148 (MQ=255)
                                                                                                  cGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCcgcg                                              >  1:1714216/1‑148 (MQ=255)
                                                                                                     ttttATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCgcgg                                           >  2:1892800/1‑148 (MQ=255)
                                                                                                     ttttATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCgcgg                                           >  2:1021545/1‑148 (MQ=255)
                                                                                                          tgatgaACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTg                                      >  1:2001492/1‑148 (MQ=255)
                                                                                                           gatgaACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTgg                                     <  1:502720/148‑1 (MQ=255)
                                                                                                           gatgaACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTgg                                     <  1:206448/148‑1 (MQ=255)
                                                                                                           gatgaACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTgg                                     >  1:650674/1‑148 (MQ=255)
                                                                                                            atgaACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGc                                    >  1:2841238/1‑148 (MQ=255)
                                                                                                               aaCAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCgg                                 >  2:2063518/1‑148 (MQ=255)
                                                                                                               aaCAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCgg                                 <  2:261936/148‑1 (MQ=255)
                                                                                                               aaCAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCgg                                 <  2:262022/148‑1 (MQ=255)
                                                                                                               aaCAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCgg                                 <  1:2160342/148‑1 (MQ=255)
                                                                                                                 cAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGAt                               <  2:2305218/148‑1 (MQ=255)
                                                                                                                   ggggTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGAttt                             <  2:1982471/148‑1 (MQ=255)
                                                                                                                   ggggTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGAttt                             >  1:1551784/1‑148 (MQ=255)
                                                                                                                     ggTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGa                           <  1:1641614/148‑1 (MQ=255)
                                                                                                                      gTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGaa                          >  1:2289555/1‑148 (MQ=255)
                                                                                                                          tgtgATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTg                      >  1:875852/1‑148 (MQ=255)
                                                                                                                           gtgATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGa                     <  1:1889807/148‑1 (MQ=255)
                                                                                                                            tgATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGaa                    >  1:3040748/1‑148 (MQ=255)
                                                                                                                             gATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAAt                   <  2:1322249/148‑1 (MQ=255)
                                                                                                                               tCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATAc                 <  1:3108189/148‑1 (MQ=255)
                                                                                                                                 aGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACgg               >  2:1606952/1‑148 (MQ=255)
                                                                                                                                  gCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGc              >  1:2498492/1‑148 (MQ=255)
                                                                                                                                  gCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACACTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGc              >  2:1666720/1‑148 (MQ=255)
                                                                                                                                     ttATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTc           >  2:515644/1‑148 (MQ=255)
                                                                                                                                     ttATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTc           >  2:739857/1‑148 (MQ=255)
                                                                                                                                        taCCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGACAGTGAATACGGCGTCCAt        >  2:2690691/1‑148 (MQ=255)
                                                                                                                                        taCCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCAt        >  2:2763007/1‑148 (MQ=255)
                                                                                                                                        taCCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCAt        <  1:1403420/148‑1 (MQ=255)
                                                                                                                                        taCCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCAt        <  2:939081/148‑1 (MQ=255)
                                                                                                                                         aCCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATc       >  2:3504265/1‑148 (MQ=255)
                                                                                                                                              ccTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCt  >  1:174854/1‑148 (MQ=255)
                                                                                                                                              ccTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCt  >  1:1969748/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCT  >  CP009273/2659449‑2659738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑