Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,660,155 G→A 5.7% Q351* (CAA→TAA)  hcaT ← putative 3‑phenylpropionic transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,660,1550GA 5.7% 822.3 / 21.9 226Q351* (CAA→TAA) hcaTputative 3‑phenylpropionic transporter
Reads supporting (aligned to +/- strand):  ref base G (99/113);  new base A (7/6);  total (107/119)
Fisher's exact test for biased strand distribution p-value = 7.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 226 total aligned reads displayed.

ATACGGACCTGACGCTGACAACACATCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGC  >  CP009273/2660010‑2660301
                                                                                                                                                 |                                                                                                                                                  
ataCGGACCTGACGCTGACAACACATCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGAt                                                                                                                                                  <  1:3375813/148‑1 (MQ=255)
  aCGGACCTGACGCTGACAACACATCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATAc                                                                                                                                                >  2:1304438/1‑148 (MQ=255)
  aCGGACCTGACGCTGACAACACATCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATAc                                                                                                                                                <  2:80110/148‑1 (MQ=255)
  aCGGACCTGACGCTGACAACACATCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATAc                                                                                                                                                >  2:754912/1‑148 (MQ=255)
  aCGGACCTGACGCTGACAACACATCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATAc                                                                                                                                                <  2:3158459/148‑1 (MQ=255)
      aCCTGACGCTGACAACACATCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGa                                                                                                                                            <  1:1499819/148‑1 (MQ=255)
         tGACGCTGACAACACATCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAAc                                                                                                                                         <  2:2444499/148‑1 (MQ=255)
           aCGCTGACAACACATCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCCGATATTGATACAGGAAACCg                                                                                                                                       >  2:3059848/1‑148 (MQ=255)
            cGCTGACAACACATCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCgg                                                                                                                                      <  2:1919700/148‑1 (MQ=255)
              cTGACAACACATCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCg                                                                                                                                    >  2:1677606/1‑148 (MQ=255)
                 acaacaCATCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGaaa                                                                                                                                 <  2:3231518/148‑1 (MQ=255)
                 acaacaCATCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTAATACAGGAAACCGGCGaaa                                                                                                                                 >  1:1747820/1‑148 (MQ=255)
                  caacaCATCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGaaaa                                                                                                                                <  2:1692585/148‑1 (MQ=255)
                   aacaCATCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAAc                                                                                                                               >  1:1399478/1‑148 (MQ=255)
                   aacaCATCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAAc                                                                                                                               >  1:3154555/1‑148 (MQ=255)
                    acacATCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACg                                                                                                                              >  2:3194828/1‑148 (MQ=255)
                      acaTCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGt                                                                                                                            >  1:1695411/1‑148 (MQ=255)
                        aTCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCa                                                                                                                          >  1:3472161/1‑148 (MQ=255)
                        aTCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCa                                                                                                                          >  2:3498060/1‑148 (MQ=255)
                           aaCAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGa                                                                                                                       <  2:1174917/148‑1 (MQ=255)
                           aaCAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGa                                                                                                                       <  2:2567571/148‑1 (MQ=255)
                            aCAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGTAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGAt                                                                                                                      >  1:1100106/1‑148 (MQ=255)
                             cAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATa                                                                                                                     >  2:3259732/1‑148 (MQ=255)
                             cAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTAATACAGGAAACCGGCGAAAACGGTCATGATa                                                                                                                     <  2:674267/148‑1 (MQ=255)
                               ggAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGc                                                                                                                   >  2:499397/1‑148 (MQ=255)
                               ggAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTAATACAGGAAACCGGCGAAAACGGTCATGATAGc                                                                                                                   <  2:2390528/148‑1 (MQ=255)
                                gAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCg                                                                                                                  <  1:112005/148‑1 (MQ=255)
                                gAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCg                                                                                                                  >  2:2124559/1‑148 (MQ=255)
                                gAGCAGATCCGCAAAATGCGCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCg                                                                                                                  <  2:3178783/148‑1 (MQ=255)
                                   cAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGTCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATa                                                                                                               <  1:1307818/148‑1 (MQ=255)
                                   cAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATa                                                                                                               <  1:1356637/148‑1 (MQ=255)
                                    aGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATAc                                                                                                              <  1:816149/148‑1 (MQ=255)
                                    aGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATAc                                                                                                              <  1:1946287/148‑1 (MQ=255)
                                        ccGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTgcc                                                                                                          >  2:730644/1‑148 (MQ=255)
                                        ccGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTgcc                                                                                                          >  2:716010/1‑148 (MQ=255)
                                          gCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTgccgc                                                                                                        <  2:546175/148‑1 (MQ=255)
                                           cAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTgccgcc                                                                                                       >  1:1664828/1‑148 (MQ=255)
                                            aaaaTGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGccc                                                                                                      <  2:2183553/148‑1 (MQ=255)
                                            aaaaTGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGccc                                                                                                      >  2:3114789/1‑148 (MQ=255)
                                              aaTGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCAt                                                                                                    >  2:736352/1‑148 (MQ=255)
                                                 gCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATcgc                                                                                                 >  1:702025/1‑148 (MQ=255)
                                                   tCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGa                                                                                               <  1:2121024/148‑1 (MQ=255)
                                                       aTCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGc                                                                                           >  1:688235/1‑148 (MQ=255)
                                                       aTCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGc                                                                                           <  2:2385891/148‑1 (MQ=255)
                                                       aTCAGCATGAGGGAACAACTTGCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGc                                                                                           <  1:3444955/148‑1 (MQ=255)
                                                          aGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCaga                                                                                        <  1:2925162/148‑1 (MQ=255)
                                                           gCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCagag                                                                                       <  2:1083678/148‑1 (MQ=255)
                                                             aTGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTa                                                                                     >  2:2809936/1‑148 (MQ=255)
                                                               gAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTaaa                                                                                   <  2:1005555/148‑1 (MQ=255)
                                                                aGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAAc                                                                                  >  1:1425376/1‑148 (MQ=255)
                                                                  ggAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCg                                                                                >  1:454928/1‑148 (MQ=255)
                                                                    aacaacTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGcc                                                                              <  1:1016016/148‑1 (MQ=255)
                                                                    aacaacTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGcc                                                                              <  1:1475552/148‑1 (MQ=255)
                                                                     acaacTTTCGGACGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCt                                                                             >  2:2944979/1‑148 (MQ=255)
                                                                      caacTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTg                                                                            <  1:2485874/148‑1 (MQ=255)
                                                                       aacTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATCGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGt                                                                           <  1:85125/148‑1 (MQ=255)
                                                                         cTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTaa                                                                         <  1:1449331/148‑1 (MQ=255)
                                                                          tttCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTaaa                                                                        <  1:3472594/148‑1 (MQ=255)
                                                                           ttCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAAc                                                                       >  2:3554103/1‑148 (MQ=255)
                                                                               ggCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGAt                                                                   <  1:2093725/148‑1 (MQ=255)
                                                                                   cAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACt                                                               <  1:3284609/148‑1 (MQ=255)
                                                                                   cAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTAATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACt                                                               >  2:1668200/1‑148 (MQ=255)
                                                                                   cAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTAATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACt                                                               >  2:2194075/1‑148 (MQ=255)
                                                                                    aaaaaCATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACtt                                                              <  2:1504211/148‑1 (MQ=255)
                                                                                      aaaCATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCg                                                            >  2:3448063/1‑148 (MQ=255)
                                                                                       aaCATTGCCGGAAGCGCCACCAGCGCCATGACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGc                                                           <  2:302695/148‑1 (MQ=255)
                                                                                           ttGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTAcc                                                       <  2:2856062/148‑1 (MQ=255)
                                                                                           ttGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTAcc                                                       <  2:131523/148‑1 (MQ=255)
                                                                                            tGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTAccc                                                      <  1:242544/148‑1 (MQ=255)
                                                                                            tGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTAccc                                                      >  1:2683004/1‑148 (MQ=255)
                                                                                               cGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTgg                                                   <  2:193603/148‑1 (MQ=255)
                                                                                                 gAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCg                                                 <  2:2681566/148‑1 (MQ=255)
                                                                                                  aaGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGa                                                >  1:1992121/1‑148 (MQ=255)
                                                                                                    gcgcCACCAGCGCCATTACCCAGAACACGCCGTTGCCCAGATATTTATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACTGCAGAGTAAACCTCCTGTAAACGTATGACTTCGCTACCATGGCGagc                                              >  1:3261229/1‑148 (MQ=255)
                                                                                                    gcgcCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGagc                                              <  2:2494984/148‑1 (MQ=255)
                                                                                                    gcgcCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGagc                                              >  1:3054437/1‑148 (MQ=255)
                                                                                                    gcgcCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGagc                                              >  2:201010/1‑148 (MQ=255)
                                                                                                         accaGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCaa                                         >  1:801669/1‑148 (MQ=255)
                                                                                                          ccaGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAat                                        >  2:2574262/1‑148 (MQ=255)
                                                                                                          ccaGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAat                                        >  1:310648/1‑148 (MQ=255)
                                                                                                                 cATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGc                                 >  1:529941/1‑148 (MQ=255)
                                                                                                                  aTTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCa                                <  1:1836160/148‑1 (MQ=255)
                                                                                                                    tACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATg                              >  2:3444725/1‑148 (MQ=255)
                                                                                                                          gAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGcc                        <  1:3133746/148‑1 (MQ=255)
                                                                                                                            acacGCCGTGGCCCAGATATTGATAGAGGAAACGGGCGAATACTGTCATGATAGCGATTCTGCCGCCCATCGCGACGGCAGTGTAAACCGCGTGTTAACGGATGTCTTCGCTACCATGGCGAGCAGCAATATTACGCTTGGCGGCCAg                      <  1:2370843/148‑1 (MQ=255)
                                                                                                                            acacGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAg                      >  1:637919/1‑148 (MQ=255)
                                                                                                                            acacGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAg                      <  2:1939531/148‑1 (MQ=255)
                                                                                                                            acacGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAg                      >  2:597955/1‑148 (MQ=255)
                                                                                                                             cacGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAgg                     <  2:1102636/148‑1 (MQ=255)
                                                                                                                             cacGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAgg                     <  2:2876974/148‑1 (MQ=255)
                                                                                                                              acGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAAGGGTCATGAGAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCGACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGt                    <  2:3161940/148‑1 (MQ=255)
                                                                                                                               cGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTg                   <  2:2713511/148‑1 (MQ=255)
                                                                                                                                gCCGTGGCCCAGATATTAATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTgg                  <  2:1747820/148‑1 (MQ=255)
                                                                                                                                    tGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGa              <  1:26449/148‑1 (MQ=255)
                                                                                                                                     ggCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGAc             <  1:1045083/148‑1 (MQ=255)
                                                                                                                                         cAGATAGTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTg         <  1:1714287/148‑1 (MQ=255)
                                                                                                                                          aGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGa        <  2:1312570/148‑1 (MQ=255)
                                                                                                                                               ttGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTg   <  1:716010/148‑1 (MQ=255)
                                                                                                                                               ttGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTg   >  1:2329936/1‑148 (MQ=255)
                                                                                                                                                tGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGc  <  2:1440859/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
ATACGGACCTGACGCTGACAACACATCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGC  >  CP009273/2660010‑2660301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑