BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,778,820	G→A	100%	intergenic (‑450/+301)	ypjC ← / ← ileY	pseudogene/tRNA‑Ile

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,778,820	0	G	A	98.7%	1013.7 / 1.9	304	intergenic (‑450/+301)	ypjC/ileY	pseudogene/tRNA‑Ile
Reads supporting (aligned to +/- strand): ref base G (1/3); new base A (124/176); total (125/179)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 307 total aligned reads displayed.

CCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGAT  >  CP009273/2778675‑2778964

cctgcGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATa                                                                                                                                                <  1:743735/148‑1 (MQ=255)

cctgcGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATa                                                                                                                                                <  2:2969683/148‑1 (MQ=255)

 ctgcGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATaa                                                                                                                                               <  2:2475662/148‑1 (MQ=255)

  tgcGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGaataat                                                                                                                                              <  1:836753/148‑1 (MQ=255)

  tgcGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGaataat                                                                                                                                              >  2:1157918/1‑148 (MQ=255)

    cggcggGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTa                                                                                                                                            <  2:2563522/148‑1 (MQ=255)

      gcggGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAAc                                                                                                                                          <  2:3491384/148‑1 (MQ=255)

         ggTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTg                                                                                                                                       >  2:2254802/1‑148 (MQ=255)

           tgtTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGAt                                                                                                                                     <  1:1789687/148‑1 (MQ=255)

              tCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTaa                                                                                                                                  >  1:593901/1‑148 (MQ=255)

                 ggTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATg                                                                                                                               <  1:131999/148‑1 (MQ=255)

                  gTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGa                                                                                                                              <  2:3427070/148‑1 (MQ=255)

                  gTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGa                                                                                                                              <  2:1005804/148‑1 (MQ=255)

                  gTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGa                                                                                                                              <  2:1645406/148‑1 (MQ=255)

                   tCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGaa                                                                                                                             <  1:1243856/148‑1 (MQ=255)

                    cAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAg                                                                                                                            <  1:1209025/148‑1 (MQ=255)

                     agtagtAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAgg                                                                                                                           <  2:1430022/148‑1 (MQ=255)

                      gtagtaGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAggg                                                                                                                          >  2:3036952/1‑148 (MQ=255)

                      gtagtaGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAggg                                                                                                                          <  1:3167800/148‑1 (MQ=255)

                        agtagATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGt                                                                                                                        <  2:805177/148‑1 (MQ=255)

                        agtagATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGt                                                                                                                        <  2:267971/148‑1 (MQ=255)

                         gtagATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGta                                                                                                                       >  2:1316069/1‑148 (MQ=255)

                            gATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTataa                                                                                                                    >  2:1697155/1‑148 (MQ=255)

                            gATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTataa                                                                                                                    >  1:1105867/1‑148 (MQ=255)

                            gATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTataa                                                                                                                    >  2:3296043/1‑148 (MQ=255)

                             aTGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGAGATTTGAATAATTAACCTGATTAAATGAAGGGGTataat                                                                                                                   >  1:1187580/1‑148 (MQ=255)

                               gTTTAAGGCGTGTAAGAGACATTTCATCCTTACTCTACGTCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTTAATAATTAACCTGATTAAATGAAGGGGTAtaataa                                                                                                                 <  2:1889985/148‑1 (MQ=255)

                               gTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTCCTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTAtaataa                                                                                                                 >  2:1262120/1‑148 (MQ=255)

                               gTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTAtaataa                                                                                                                 <  1:3122238/148‑1 (MQ=255)

                                 ttAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAAt                                                                                                               >  2:928075/1‑148 (MQ=255)

                                 ttAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAAt                                                                                                               >  1:1001801/1‑148 (MQ=255)

                                 ttAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAAt                                                                                                               >  2:1727826/1‑148 (MQ=255)

                                  tAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATg                                                                                                              <  1:3031771/148‑1 (MQ=255)

                                   aaGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGa                                                                                                             <  2:3442271/148‑1 (MQ=255)

                                     ggCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGata                                                                                                           >  1:800742/1‑148 (MQ=255)

                                      gCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGataa                                                                                                          <  1:2759709/148‑1 (MQ=255)

                                          ggCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATAct                                                                                                      <  2:2299632/148‑1 (MQ=255)

                                          ggCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATAct                                                                                                      <  2:2788266/148‑1 (MQ=255)

                                              gagaattttAATCCTTACTCTACGGCATTGTTCTACATACATTTGTTGTGGTACTCTCTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTTTATTATTATCCTGATTAAATGAAGGGTTATAATAAATGATAATACTCTgg                                                                                                  <  1:571839/142‑1 (MQ=255)

                                               agaCATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGc                                                                                                 >  1:1855221/1‑148 (MQ=255)

                                               agaCATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGc                                                                                                 >  1:306191/1‑148 (MQ=255)

                                                gaCATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCt                                                                                                <  2:1152415/148‑1 (MQ=255)

                                                 aCATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCtt                                                                                               >  1:1851858/1‑148 (MQ=255)

                                                   aTTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTa                                                                                             <  1:1968485/148‑1 (MQ=255)

                                                    tttCATCCTTACTCTACGGTATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTAt                                                                                            <  2:2505854/148‑1 (MQ=255)

                                                    tttCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTAt                                                                                            <  2:2183775/148‑1 (MQ=255)

                                                     ttCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATc                                                                                           >  2:1534799/1‑148 (MQ=255)

                                                      tCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCg                                                                                          >  1:814738/1‑148 (MQ=255)

                                                            ttACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAAtt                                                                                    >  2:2635444/1‑148 (MQ=255)

                                                            ttACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAAtt                                                                                    <  1:2533264/148‑1 (MQ=255)

                                                            ttACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAAtt                                                                                    >  2:32612/1‑148 (MQ=255)

                                                            ttACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAAtt                                                                                    >  1:1710691/1‑148 (MQ=255)

                                                              aCTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTAc                                                                                  >  2:1554422/1‑148 (MQ=255)

                                                              aCTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTAc                                                                                  <  1:1619380/148‑1 (MQ=255)

                                                                  tACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTaa                                                                              <  1:2152640/148‑1 (MQ=255)

                                                                   aCGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAAt                                                                             >  1:2006844/1‑148 (MQ=255)

                                                                    cGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAAtt                                                                            <  1:2739770/148‑1 (MQ=255)

                                                                     ggCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTc                                                                           >  2:732426/1‑148 (MQ=255)

                                                                       cATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCca                                                                         <  2:592546/148‑1 (MQ=255)

                                                                       cATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCca                                                                         >  2:2893289/1‑148 (MQ=255)

                                                                       cATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCca                                                                         >  1:2698984/1‑148 (MQ=255)

                                                                        aTTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCcac                                                                        <  1:2106874/148‑1 (MQ=255)

                                                                        aTTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCcac                                                                        <  1:2056823/148‑1 (MQ=255)

                                                                            ttCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGt                                                                    <  1:2624004/148‑1 (MQ=255)

                                                                                  atacatTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCaa                                                              >  2:1314684/1‑148 (MQ=255)

                                                                                  atacatTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCaa                                                              >  2:3083440/1‑148 (MQ=255)

                                                                                   tacatTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTACGCAAt                                                             >  1:1529117/1‑148 (MQ=255)

                                                                                   tacatTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAAt                                                             >  2:1512923/1‑148 (MQ=255)

                                                                                   tacatTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAAt                                                             >  1:1438552/1‑148 (MQ=255)

                                                                                     catTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAAttt                                                           >  1:470045/1‑148 (MQ=255)

                                                                                       ttGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGc                                                         <  1:663009/148‑1 (MQ=255)

                                                                                        tGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGcc                                                        >  1:1921801/1‑148 (MQ=255)

                                                                                          gTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCg                                                      <  1:2071403/148‑1 (MQ=255)

                                                                                           ttGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGc                                                     <  2:2589140/148‑1 (MQ=255)

                                                                                               ggTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTgg                                                 >  1:3424068/1‑148 (MQ=255)

                                                                                                  aCTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCAt                                              <  1:129904/148‑1 (MQ=255)

                                                                                                      aCTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCa                                          <  1:2854650/148‑1 (MQ=255)

                                                                                                      aCTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCa                                          <  1:1081450/148‑1 (MQ=255)

                                                                                                       cTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAg                                         >  2:634935/1‑148 (MQ=255)

                                                                                                        ttATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAgg                                        >  2:2615677/1‑148 (MQ=255)

                                                                                                        ttATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAgg                                        <  1:229623/148‑1 (MQ=255)

                                                                                                              tcaGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCAtttt                                  <  2:160026/148‑1 (MQ=255)

                                                                                                                  tGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTGTAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTcc                              <  1:1139506/148‑1 (MQ=255)

                                                                                                                  tGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTcc                              <  2:1214160/148‑1 (MQ=255)

                                                                                                                      cGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGt                          <  1:2813024/148‑1 (MQ=255)

                                                                                                                      cGAACAGAGAATAGTACAGTGATTTGAATAATTAACCTTATTAAATGAATGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGt                          <  2:1063481/148‑1 (MQ=255)

                                                                                                                       gAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTa                         <  1:1678743/148‑1 (MQ=255)

                                                                                                                       gAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTa                         <  2:50038/148‑1 (MQ=255)

                                                                                                                        aaCAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTAc                        <  2:374825/148‑1 (MQ=255)

                                                                                                                              gaATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGa                  <  1:2994500/148‑1 (MQ=255)

                                                                                                                                aTAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAAt                >  1:2246566/1‑148 (MQ=255)

                                                                                                                                 tAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATg               <  2:2077106/148‑1 (MQ=255)

                                                                                                                                  aGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGa              >  1:3263998/1‑148 (MQ=255)

                                                                                                                                    ttCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGt            <  1:3083440/148‑1 (MQ=255)

                                                                                                                                         tGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGa       >  1:1548952/1‑148 (MQ=255)

                                                                                                                                           aTTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATg     <  1:3193159/148‑1 (MQ=255)

                                                                                                                                             ttGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGa   <  1:809703/148‑1 (MQ=255)

                                                                                                                                             ttGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGa   >  2:583555/1‑148 (MQ=255)

                                                                                                                                              tGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGAt  >  1:2430996/1‑148 (MQ=255)

                                                                                                                                              tGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGAt  >  1:2430881/1‑148 (MQ=255)

CCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGAT  >  CP009273/2778675‑2778964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`