BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,801,706	C→A	100%	pseudogene (32/1178 nt)	ygaY →	pseudogene, major facilitator transporter superfamily;putative transport; Not classified; putative transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,801,706	0	C	A	96.1%	620.5 / 13.5	202	pseudogene (32/1178 nt)	ygaY	pseudogene, major facilitator transporter superfamily;putative transport; Not classified; putative transport protein
Reads supporting (aligned to +/- strand): ref base C (6/2); new base A (120/74); total (126/76)
Fisher's exact test for biased strand distribution p-value = 7.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 202 total aligned reads displayed.

TCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCCGCGCAGTTGGGCTA  >  CP009273/2801564‑2801850

tCATGCCTACACACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGa                                                                                                                                             <  2:1033789/148‑1 (MQ=255)

    gCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGt                                                                                                                                         >  1:965745/1‑148 (MQ=255)

     ccTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTg                                                                                                                                        <  1:1418185/148‑1 (MQ=255)

      cTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGc                                                                                                                                       <  2:3192465/148‑1 (MQ=255)

         cAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCGTCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGa                                                                                                                                    >  2:928137/1‑148 (MQ=255)

          aaaCCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGAt                                                                                                                                   >  2:3492401/1‑148 (MQ=255)

           aaCCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATg                                                                                                                                  <  1:1329594/148‑1 (MQ=255)

           aaCCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATg                                                                                                                                  <  1:3340890/148‑1 (MQ=255)

             ccACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTc                                                                                                                                >  2:509184/1‑148 (MQ=255)

               ccaTCGCACATTTCAACACTCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTa                                                                                                                              <  2:2022390/147‑1 (MQ=255)

                caTCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTAt                                                                                                                             >  1:627698/1‑148 (MQ=255)

                caTCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTAt                                                                                                                             <  2:1579153/148‑1 (MQ=255)

                caTCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTAt                                                                                                                             <  2:1579573/148‑1 (MQ=255)

                caTCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTAt                                                                                                                             >  2:1990096/1‑148 (MQ=255)

                   cGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGc                                                                                                                          >  1:10016/1‑148 (MQ=255)

                        aTTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCg                                                                                                                     >  1:512656/1‑148 (MQ=255)

                        aTTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCg                                                                                                                     >  1:2299852/1‑148 (MQ=255)

                        aTTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCg                                                                                                                     <  2:2182000/148‑1 (MQ=255)

                        aTTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCg                                                                                                                     >  1:2957562/1‑148 (MQ=255)

                         tttCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCgg                                                                                                                    >  2:1431311/1‑148 (MQ=255)

                          ttCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGt                                                                                                                   <  2:1992809/148‑1 (MQ=255)

                                 atctctctTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTACTCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCgg                                                                                                            <  2:2501233/148‑1 (MQ=255)

                                 atctatctTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCgg                                                                                                            >  2:3433641/1‑148 (MQ=255)

                                  tctatctTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGt                                                                                                           <  2:359744/148‑1 (MQ=255)

                                  tctatctTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGt                                                                                                           <  2:1336527/148‑1 (MQ=255)

                                   ctatctTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTa                                                                                                          >  1:175861/1‑148 (MQ=255)

                                     atctTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGc                                                                                                        <  2:3293063/148‑1 (MQ=255)

                                     atctTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGc                                                                                                        <  2:2035956/148‑1 (MQ=255)

                                          tCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTa                                                                                                   >  2:3088902/1‑148 (MQ=255)

                                          tCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTa                                                                                                   >  1:3495465/1‑148 (MQ=255)

                                              cccATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTa                                                                                               >  2:1720728/1‑148 (MQ=255)

                                                  tatTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTAc                                                                                           >  1:2392028/1‑148 (MQ=255)

                                                   atTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACg                                                                                          <  2:3523307/148‑1 (MQ=255)

                                                    ttCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCACGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGc                                                                                         >  1:2024799/1‑148 (MQ=255)

                                                     tcatcaACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTACTCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGcc                                                                                        <  2:3394992/148‑1 (MQ=255)

                                                     tcatcaACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCCGTAACTATTACGcc                                                                                        >  2:3066691/1‑148 (MQ=255)

                                                     tcatcaACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGcc                                                                                        >  2:2647214/1‑148 (MQ=255)

                                                     tcatcaACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGcc                                                                                        <  1:1077993/148‑1 (MQ=255)

                                                     tcaacaACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGcc                                                                                        <  2:2406607/148‑1 (MQ=255)

                                                      catcaACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGccc                                                                                       <  1:1501421/148‑1 (MQ=255)

                                                        tcaACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGAAGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAg                                                                                     >  2:1894411/1‑148 (MQ=255)

                                                              tCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTg                                                                               <  2:58161/148‑1 (MQ=255)

                                                              tCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTg                                                                               <  2:3457595/148‑1 (MQ=255)

                                                                cGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCt                                                                             <  2:2971895/148‑1 (MQ=255)

                                                                 gCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTc                                                                            >  1:2258900/1‑148 (MQ=255)

                                                                  cTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCg                                                                           <  1:719047/148‑1 (MQ=255)

                                                                  cTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCg                                                                           >  2:1194782/1‑148 (MQ=255)

                                                                    attattGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGac                                                                         >  2:2611043/1‑148 (MQ=255)

                                                                     ttattGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGaca                                                                        >  2:3380872/1‑148 (MQ=255)

                                                                        ttGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCa                                                                     >  2:2082971/1‑148 (MQ=255)

                                                                             ttCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATcgcg                                                                >  1:3387087/1‑148 (MQ=255)

                                                                             ttCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATcgcg                                                                >  1:1662289/1‑148 (MQ=255)

                                                                                cAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGt                                                             <  2:3328690/148‑1 (MQ=255)

                                                                                 aGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTa                                                            >  2:2140820/1‑148 (MQ=255)

                                                                                  gCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTaa                                                           >  1:1544269/1‑148 (MQ=255)

                                                                                     tAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACtt                                                        >  1:1454461/1‑148 (MQ=255)

                                                                                     tAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACtt                                                        >  1:908080/1‑148 (MQ=255)

                                                                                      aaTCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACttt                                                       <  2:2957562/148‑1 (MQ=255)

                                                                                           tcaCCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCt                                                  >  1:1348444/1‑148 (MQ=255)

                                                                                            caCCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCtt                                                 <  2:918619/148‑1 (MQ=255)

                                                                                            caCCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCtt                                                 >  2:905248/1‑148 (MQ=255)

                                                                                             aCCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCttt                                                <  1:2345607/148‑1 (MQ=255)

                                                                                              ccggatgaACACAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTc                                               <  2:2344784/145‑1 (MQ=255)

                                                                                                 gatgaACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGc                                            >  2:689716/1‑148 (MQ=255)

                                                                                                 gatgaACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGc                                            >  2:109593/1‑148 (MQ=255)

                                                                                                    gaACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAg                                         >  1:1261569/1‑148 (MQ=255)

                                                                                                     aaCAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCCCCGGTCTGGCGGTAGCCCGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGt                                        >  2:3151388/1‑148 (MQ=255)

                                                                                                      aCAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGtt                                       >  1:2632957/1‑148 (MQ=255)

                                                                                                      aCAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGtt                                       <  2:3495465/148‑1 (MQ=255)

                                                                                                          aaaTAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGc                                   >  2:3516393/1‑148 (MQ=255)

                                                                                                          aaaTAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGc                                   <  1:69825/148‑1 (MQ=255)

                                                                                                           aataatGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCCTTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCa                                  >  1:2835663/1‑148 (MQ=255)

                                                                                                           aataatGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCa                                  >  1:3047758/1‑148 (MQ=255)

                                                                                                            ataatGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAg                                 >  2:875412/1‑148 (MQ=255)

                                                                                                            ataatGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAg                                 >  2:1363311/1‑148 (MQ=255)

                                                                                                            ataatGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCAGCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAg                                 >  2:2029969/1‑148 (MQ=255)

                                                                                                                tGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCtt                             <  2:2893192/148‑1 (MQ=255)

                                                                                                                 gACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCttt                            >  1:3216171/1‑148 (MQ=255)

                                                                                                                  aCTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTa                           <  2:1923125/148‑1 (MQ=255)

                                                                                                                  aCTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTa                           >  1:399758/1‑148 (MQ=255)

                                                                                                                  aCTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTa                           >  1:1526083/1‑148 (MQ=255)

                                                                                                                   cTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTAt                          >  1:731523/1‑148 (MQ=255)

                                                                                                                    tAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTAtt                         >  1:1931340/1‑148 (MQ=255)

                                                                                                                     aaaCCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTg                        <  2:499436/148‑1 (MQ=255)

                                                                                                                      aaCCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGt                       >  1:1828426/1‑148 (MQ=255)

                                                                                                                      aaCCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGt                       >  1:2278906/1‑148 (MQ=255)

                                                                                                                      aaCCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGt                       <  2:1756223/148‑1 (MQ=255)

                                                                                                                       aCCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGtt                      >  1:472160/1‑148 (MQ=255)

                                                                                                                         cTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTAc                    >  1:645182/1‑148 (MQ=255)

                                                                                                                          tAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTAcc                   >  1:1109519/1‑148 (MQ=255)

                                                                                                                           aaTCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTAccg                  >  1:2085369/1‑148 (MQ=255)

                                                                                                                               aTGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACcgccgc              >  1:240849/1‑148 (MQ=255)

                                                                                                                                 gAGCTTAGCCCGGAGATGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCCgcgc            >  2:3344538/1‑148 (MQ=255)

                                                                                                                                  aGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCCGCGCa           >  2:1232216/1‑148 (MQ=255)

                                                                                                                                  aGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCCGCGCa           >  2:2295101/1‑148 (MQ=255)

                                                                                                                                    cTTAGCCCGGCGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCCGCGCAGt         >  2:780877/1‑148 (MQ=255)

                                                                                                                                    cTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCCGCGCAGt         <  2:398910/148‑1 (MQ=255)

                                                                                                                                        gCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCCGCGCAGTTggg     <  1:2470301/148‑1 (MQ=255)

                                                                                                                                         cccGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCCGCGCAGTTGGGc    >  2:1546166/1‑148 (MQ=255)

                                                                                                                                         cccGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCCGCGCAGTTGGGc    <  1:3433641/148‑1 (MQ=255)

TCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCCGCGCAGTTGGGCTA  >  CP009273/2801564‑2801850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`