Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,832,598 T→G 14.5% K9T (AAG→ACG)  ascG ← asc operon transcriptional repressor; prpBC operon repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,832,5980TG14.5% 372.6 / 11.2 196K9T (AAG→ACG) ascGasc operon transcriptional repressor; prpBC operon repressor
Reads supporting (aligned to +/- strand):  ref base T (55/107);  new base G (31/3);  total (86/110)
Fisher's exact test for biased strand distribution p-value = 5.79e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 203 total aligned reads displayed.

CTCTTCGCCGACAGATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTC  >  CP009273/2832451‑2832745
                                                                                                                                                   |                                                                                                                                                   
ctctTCGCCGACAGATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCNTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCt                                                                                                                                                     <  1:1803862/148‑1 (MQ=255)
    tCGCCGACAGATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGc                                                                                                                                                 >  2:2594828/1‑148 (MQ=255)
         gACAGATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACtt                                                                                                                                            <  2:2095291/148‑1 (MQ=255)
          aCAGATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGTAACCCCGGCGCGCGTCGccccatc                                                                                                                                           >  1:1941525/1‑143 (MQ=255)
          aCAGATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTc                                                                                                                                           <  1:2097223/148‑1 (MQ=255)
           cAGATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCGTTTGAAACCCCCGCGCGCGTCGCCCCTTcc                                                                                                                                          >  1:1393222/1‑148 (MQ=255)
            aGATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGTAACCCCCGCGCGCGGCGCCCCATCCa                                                                                                                                         >  1:2683084/1‑148 (MQ=255)
              aTTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGc                                                                                                                                       <  2:514017/148‑1 (MQ=255)
              aTTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGc                                                                                                                                       >  1:1643848/1‑148 (MQ=255)
                tGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCAt                                                                                                                                     <  2:1323477/148‑1 (MQ=255)
                tGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCGTTTGAAACCCCGGCGCGCGTCGCCACTTCCAGCAt                                                                                                                                     >  1:1483473/1‑148 (MQ=255)
                       cAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCgtcgtc                                                                                                                              <  2:3506531/148‑1 (MQ=255)
                        aGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGtca                                                                                                                             <  2:869903/148‑1 (MQ=255)
                        aGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGtca                                                                                                                             <  2:1887432/148‑1 (MQ=255)
                          cAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGtcatc                                                                                                                           >  2:3545213/1‑148 (MQ=255)
                          cAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCGTCGCCACATACAGCAGCGTCGtcatc                                                                                                                           >  1:293030/1‑148 (MQ=255)
                            aGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTcatcat                                                                                                                         <  2:428786/148‑1 (MQ=255)
                                tGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTc                                                                                                                     <  2:758346/148‑1 (MQ=255)
                                  gACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCAt                                                                                                                   >  1:1733595/1‑148 (MQ=255)
                                  gACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCCGCGCGCGTCGCCACATCCAGCATCGTCGTCAAAATTTTAAt                                                                                                                   >  1:3493389/1‑148 (MQ=255)
                                      gTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGTAACCCCGGCGCGCGTCGCCACATCCAGCATCGTCGTCATCATTTTAATACCt                                                                                                               >  1:1095984/1‑148 (MQ=255)
                                      gTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCGGCGCCACTTCCAGCATCGTCGTCATAAATTTAATccca                                                                                                               >  1:1977841/1‑147 (MQ=255)
                                        aaCCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCCGCGCGCTTCGCCACTTCCACCAGCGGCGTCATCAATTTAAtaacatt                                                                                                             >  1:987569/1‑142 (MQ=255)
                                        aaCCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCGTTTGTAACCCCCGCGCGCGTCGCCCCATCCCGCAACGGCGGCAACAATTTAAAACCttt                                                                                                             >  1:396285/1‑148 (MQ=255)
                                         aCCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTa                                                                                                            <  1:1787263/148‑1 (MQ=255)
                                         aCCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTa                                                                                                            <  2:865452/148‑1 (MQ=255)
                                         aCCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCCGCGCGCGTCGCCACGTCCCCCCTCGTCGTCCAAAATTTAATCCCATTa                                                                                                            >  1:2430036/1‑148 (MQ=255)
                                          ccGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGGCACTTCCAGCATCGTCGTCATCATTTTCATGCCTTTac                                                                                                           >  2:3127912/1‑148 (MQ=255)
                                          ccGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTac                                                                                                           <  2:3342055/148‑1 (MQ=255)
                                             ctctctTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACg                                                                                                        >  1:2311923/1‑148 (MQ=255)
                                               ctctTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCa                                                                                                      <  2:696495/148‑1 (MQ=255)
                                                 cttctACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAAt                                                                                                    <  1:1874917/148‑1 (MQ=255)
                                                  ttctACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATc                                                                                                   <  1:1560332/148‑1 (MQ=255)
                                                  ttctACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACATCCAGCATCGTCGTCATCATTTTCATCCCGTTACACGCAATc                                                                                                   >  2:3535302/1‑148 (MQ=255)
                                                   tctACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCCCTTCCAGCATCGTCGTCATCATTTTCATCCCTTTAAACGCCAtaa                                                                                                  >  1:794436/1‑146 (MQ=255)
                                                      aCCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAAACAACg                                                                                               >  2:2764227/1‑148 (MQ=255)
                                                      aCCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCGTTTGGAACCCCGGCGCGCGGCGCCACTTCCAGCCACGTCGTCAAAAATTTAATCCCATTACAAGCAATCAACg                                                                                               >  1:153987/1‑148 (MQ=255)
                                                         gCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAg                                                                                            <  2:1017948/148‑1 (MQ=255)
                                                            tGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAgtgt                                                                                         <  2:1643848/148‑1 (MQ=255)
                                                            tGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCCGCGCGCTTCGCCACATCCAGCATCGTCGTCAAAATTTTCATCCCTTTACACCCAAAAAACGCAgtgt                                                                                         >  2:1221994/1‑148 (MQ=255)
                                                                acacGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTg                                                                                     <  1:1221379/148‑1 (MQ=255)
                                                                   cgcgATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCAc                                                                                  >  2:2350444/1‑148 (MQ=255)
                                                                    gcgATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCAcc                                                                                 <  2:2738582/148‑1 (MQ=255)
                                                                      gATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGt                                                                               <  2:121146/148‑1 (MQ=255)
                                                                       aTCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGTAACCCCGGCGCGCGTCGCCACATCCCGCATCGTCGTCCTAAATTTAATACCTTTTAACGCCATAAACGCACTTTAAAGCACCGtt                                                                              >  1:1414170/1‑148 (MQ=255)
                                                                           ttAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTTAAACCCCGGCGCGCTTCGCCACATCCAGCATCGTGGTCATCATTTTGATACCCTTACAAGCAATAAACCCAGAGTTCTGGACCCTTTTcc                                                                          >  2:906338/1‑148 (MQ=255)
                                                                           ttAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCGTTTGGATCCCCGGCGCGCTTCCCCACTTGCACGCGCGGCGGCATCATTGTCCACGCGTGACACGCAAACCCCCCCGTGTCCGGCCCAGTTTGcc                                                                          >  2:1824996/1‑148 (MQ=255)
                                                                            tAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTTTAACCCCCGCGCGCGTGGCCACTTCCAGCAACGTCGTCATCAATTTAATCCCTTTAAACGCAATCAACGCCGTGTTCTGCCCCCttttgcc                                                                         >  1:3373723/1‑144 (MQ=255)
                                                                            tAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCg                                                                         <  1:942095/148‑1 (MQ=255)
                                                                              gTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATACCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGAt                                                                       >  2:2490921/1‑148 (MQ=255)
                                                                               tctcCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGAtt                                                                      >  2:1395509/1‑148 (MQ=255)
                                                                               tctcCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACATCCACCATCGTCGTCATCAATTTCATACCGTTACAAGCAATCAACCCAGTGTACTGCACCGTTTTGCGAtt                                                                      >  2:2394580/1‑148 (MQ=255)
                                                                                ctcCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCGTCGCCACTTCCAGCATAGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTg                                                                     >  2:954746/1‑148 (MQ=255)
                                                                                 tcCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGt                                                                    <  1:2240608/148‑1 (MQ=255)
                                                                                 tcCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCCGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCGTTAAAAGCAATCAAAGCACTGTACTGCACCCTTTGCCGATTGt                                                                    >  2:171558/1‑148 (MQ=255)
                                                                                   cTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTcc                                                                  <  1:1382801/148‑1 (MQ=255)
                                                                                   cTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACGTCCAGCATCGTCGTCATCATTTTCATACCGTTTAACGCCATAAACCCAGTGTACTGGACCGGTTGGCGATTGTcc                                                                  >  2:1515757/1‑148 (MQ=255)
                                                                                     ggCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGGGTACTGCACCCTTTGCCGATTGTCCtt                                                                >  2:2013196/1‑148 (MQ=255)
                                                                                      gCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTg                                                               <  1:2705804/148‑1 (MQ=255)
                                                                                        tGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGca                                                             <  1:1448802/148‑1 (MQ=255)
                                                                                            gTAGCCATTACCTGAACGCACGCGGGAAACGGTCGCTTTTGACACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAAt                                                         <  2:3033064/148‑1 (MQ=255)
                                                                                             tAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATc                                                        <  2:1156712/148‑1 (MQ=255)
                                                                                                 cATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcggcg                                                    <  2:2554695/148‑1 (MQ=255)
                                                                                                 cATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcggcg                                                    <  2:246098/148‑1 (MQ=255)
                                                                                                   ttACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCggg                                                  <  1:662080/148‑1 (MQ=255)
                                                                                                   ttACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTAATCCCTTTACACGCAATCAACGCAGTGTACGGCACCGTTTGGCGAATGTCCATGCACAAAAGGCggg                                                  >  2:777843/1‑148 (MQ=255)
                                                                                                    tACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCGTCGCCACGTCCAGCATCGTCGTCATCATTTTCATCCCGTTACACGCAAACAACGCAGTGTACTGCACCGTTTGCCGATTTTACTTTGACAATAGGCgggg                                                 >  1:2061601/1‑147 (MQ=255)
                                                                                                      ccTGAAAGCACGCGGGACACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTCCCCGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGaaa                                               <  2:2898242/148‑1 (MQ=255)
                                                                                                         gAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAat                                            <  2:1812177/148‑1 (MQ=255)
                                                                                                           aaGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAatat                                          <  2:1391265/148‑1 (MQ=255)
                                                                                                             gcccgcgGGAAACGGACGCTTTTGAACCCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTc                                        <  2:2555563/145‑1 (MQ=255)
                                                                                                             gCACGCGGGAAACGGTCGCGTTTTAAACCCCGGCGCGCTTCGCCACATCCAGCAACGTCGTCATCATTTTCATCCCTTTACACGCAAACAACGCAGTGTACTGCACCCGTTGCCGATTGTCCTTGCACAATCGGCGGGACAAATATTc                                        >  2:1808829/1‑148 (MQ=255)
                                                                                                              cACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCa                                       <  2:916065/148‑1 (MQ=255)
                                                                                                              cACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATACCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGTCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCa                                       >  2:1595790/1‑148 (MQ=255)
                                                                                                                   gggAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGa                                  <  2:3557962/148‑1 (MQ=255)
                                                                                                                      aaaCGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGGTTGCCGATTGTCCTTGCACAATCGGCGGGACCAATATTCAGGTGACCg                               >  2:2254068/1‑148 (MQ=255)
                                                                                                                         cGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGtt                            <  2:3366232/148‑1 (MQ=255)
                                                                                                                            tCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTca                         <  2:1733595/148‑1 (MQ=255)
                                                                                                                            tCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTca                         <  2:3493326/148‑1 (MQ=255)
                                                                                                                              gCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTcaca                       <  2:293030/148‑1 (MQ=255)
                                                                                                                               cTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACaa                      >  1:1429667/1‑148 (MQ=255)
                                                                                                                                ttttGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACaaa                     <  1:808261/148‑1 (MQ=255)
                                                                                                                                ttttGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACaaa                     <  1:2590128/148‑1 (MQ=255)
                                                                                                                                ttttGAAACCCCGGCGCGCGTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACaaa                     <  1:1219552/148‑1 (MQ=255)
                                                                                                                                 tttGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAat                    <  1:3092995/148‑1 (MQ=255)
                                                                                                                                   tGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAatat                  <  1:1333946/148‑1 (MQ=255)
                                                                                                                                     aaaCCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATaa                >  1:2849899/1‑148 (MQ=255)
                                                                                                                                     aaaCCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATaa                >  1:1074931/1‑148 (MQ=255)
                                                                                                                                     aaaCCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATaa                <  2:3203553/148‑1 (MQ=255)
                                                                                                                                       aCCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATaaaa              >  2:2241207/1‑148 (MQ=255)
                                                                                                                                        ccccGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATaaaaa             <  2:1095984/148‑1 (MQ=255)
                                                                                                                                          ccGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAAt           >  2:885076/1‑148 (MQ=255)
                                                                                                                                           cGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATg          <  1:837349/148‑1 (MQ=255)
                                                                                                                                            ggCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGa         <  1:2488615/148‑1 (MQ=255)
                                                                                                                                             gcgcgcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGaa        <  2:1483447/148‑1 (MQ=255)
                                                                                                                                             gcgcgcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGaa        <  2:1483477/148‑1 (MQ=255)
                                                                                                                                               gcgcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACa      <  2:2277057/148‑1 (MQ=255)
                                                                                                                                                 gcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAt    <  2:1771186/148‑1 (MQ=255)
                                                                                                                                                   ttCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACACTTc  >  2:56016/1‑148 (MQ=255)
                                                                                                                                                   ttCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTc  >  2:3037272/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CTCTTCGCCGACAGATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTC  >  CP009273/2832451‑2832745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑