Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,835,094 C→A 100% P249Q (CCG→CAG)  ascB → cryptic 6‑phospho‑beta‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,835,0940CA98.3% 562.5 / 0.8 178P249Q (CCG→CAG) ascBcryptic 6‑phospho‑beta‑glucosidase
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base A (111/62);  total (113/65)
Fisher's exact test for biased strand distribution p-value = 2.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 178 total aligned reads displayed.

ATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCCGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGAT  >  CP009273/2834950‑2835236
                                                                                                                                                |                                                                                                                                              
atcaggatcaggTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGa                                                                                                                                             >  2:3367650/1‑148 (MQ=255)
 tcaggatcaggTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGaa                                                                                                                                            >  1:2236959/1‑148 (MQ=255)
   aggatcaggTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGa                                                                                                                                          >  2:864641/1‑148 (MQ=255)
   aggatcaggTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGa                                                                                                                                          <  1:1637732/148‑1 (MQ=255)
          ggTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTgg                                                                                                                                   >  2:3262325/1‑148 (MQ=255)
           gTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTggg                                                                                                                                  >  1:1101288/1‑148 (MQ=255)
           gTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTggg                                                                                                                                  >  2:2056887/1‑148 (MQ=255)
           gTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTggg                                                                                                                                  >  2:2056846/1‑148 (MQ=255)
              aaaTATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGggcgg                                                                                                                               >  1:3413668/1‑148 (MQ=255)
               aaTATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGggcggc                                                                                                                              >  2:594130/1‑148 (MQ=255)
                atatCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGgcggcg                                                                                                                             >  2:3441302/1‑148 (MQ=255)
                atatCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGgcggcg                                                                                                                             <  1:2979233/148‑1 (MQ=255)
                atatCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGgcggcg                                                                                                                             >  1:7062/1‑148 (MQ=255)
                  atCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCt                                                                                                                           >  1:2827805/1‑148 (MQ=255)
                    cAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTgg                                                                                                                         <  2:3205250/148‑1 (MQ=255)
                    cAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTgg                                                                                                                         >  1:3377273/1‑148 (MQ=255)
                       gCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGgaga                                                                                                                      >  1:3051376/1‑148 (MQ=255)
                        ccGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGaa                                                                                                                     <  1:1189316/148‑1 (MQ=255)
                          gcgcATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAGGATGTCTGGGCGGCGCTGGAGAAAg                                                                                                                   >  1:2150506/1‑148 (MQ=255)
                          gcgcATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAg                                                                                                                   >  1:2150774/1‑148 (MQ=255)
                            gcATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGAc                                                                                                                 <  1:549645/148‑1 (MQ=255)
                            gcATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGAc                                                                                                                 <  2:1872491/148‑1 (MQ=255)
                                cACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCggg                                                                                                             >  2:2531653/1‑148 (MQ=255)
                                cACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCggg                                                                                                             >  1:1050945/1‑148 (MQ=255)
                                cACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCggg                                                                                                             >  2:1334657/1‑148 (MQ=255)
                                  ccAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGaa                                                                                                           >  2:2746550/1‑148 (MQ=255)
                                     gCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAAc                                                                                                        <  2:2648228/148‑1 (MQ=255)
                                     gCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAAc                                                                                                        >  1:176848/1‑148 (MQ=255)
                                     gCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAAc                                                                                                        <  2:2648253/148‑1 (MQ=255)
                                     gCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAAc                                                                                                        <  2:1666996/148‑1 (MQ=255)
                                      cTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAAcc                                                                                                       <  1:1882651/148‑1 (MQ=255)
                                          ttGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGACAACCTGtt                                                                                                   >  2:2375898/1‑148 (MQ=255)
                                              cAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGtttttt                                                                                               <  2:3527673/148‑1 (MQ=255)
                                              cAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGtttttt                                                                                               <  1:3140445/148‑1 (MQ=255)
                                              cAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGtttttt                                                                                               <  1:412778/148‑1 (MQ=255)
                                               aGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTa                                                                                              <  2:244274/148‑1 (MQ=255)
                                                 tGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATc                                                                                            >  2:1077278/1‑148 (MQ=255)
                                                       aGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGAtgtg                                                                                      <  1:3255592/148‑1 (MQ=255)
                                                        gCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGc                                                                                     <  1:515233/148‑1 (MQ=255)
                                                              aaaaTCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGgcgc                                                                               >  2:2832253/1‑148 (MQ=255)
                                                                aaTCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCCGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCgg                                                                             <  2:1236430/148‑1 (MQ=255)
                                                                aaTCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCgg                                                                             >  1:2782792/1‑148 (MQ=255)
                                                                aaTCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCgg                                                                             <  2:2059820/148‑1 (MQ=255)
                                                                aaTCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCgg                                                                             >  1:1036725/1‑148 (MQ=255)
                                                                  tCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCgggg                                                                           >  2:938115/1‑148 (MQ=255)
                                                                   cGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGc                                                                          <  2:1475692/148‑1 (MQ=255)
                                                                     cccATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCAc                                                                        >  2:2684161/1‑148 (MQ=255)
                                                                        aTGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGAAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGAGCGGGGCACGTa                                                                     >  1:3016616/1‑148 (MQ=255)
                                                                         tGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTAt                                                                    >  1:2294333/1‑148 (MQ=255)
                                                                           aGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATcc                                                                  >  2:1613313/1‑148 (MQ=255)
                                                                           aGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATcc                                                                  >  2:1611394/1‑148 (MQ=255)
                                                                             gTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCgg                                                                <  1:1912385/148‑1 (MQ=255)
                                                                             gTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCgg                                                                >  1:3214678/1‑148 (MQ=255)
                                                                              ttAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGc                                                               >  2:438334/1‑148 (MQ=255)
                                                                              ttAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGc                                                               >  2:1915922/1‑148 (MQ=255)
                                                                              ttAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCCGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGc                                                               <  1:1979380/148‑1 (MQ=255)
                                                                               tAACCAGCAAAATAAGGTGGGCTGAATGCTGGCGGGCGGTAACTTCTACACATACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACATGTTTTTTATCTAAGATCAGGCGCGGGGCACGAATCCGGCt                                                              >  2:1531068/1‑148 (MQ=255)
                                                                                 aCCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTa                                                            <  1:2970401/148‑1 (MQ=255)
                                                                                  cccGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTAc                                                           >  1:991902/1‑148 (MQ=255)
                                                                                   ccGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTAct                                                          <  1:940214/148‑1 (MQ=255)
                                                                                   ccGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTAct                                                          <  1:526353/148‑1 (MQ=255)
                                                                                   ccGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTAct                                                          <  2:2827805/148‑1 (MQ=255)
                                                                                      cAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTg                                                       >  1:3337073/1‑148 (MQ=255)
                                                                                         aaTCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCGGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGccc                                                    >  1:3173715/1‑148 (MQ=255)
                                                                                          aTCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCcg                                                   <  1:2168174/148‑1 (MQ=255)
                                                                                           tCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCcgc                                                  >  1:3239186/1‑148 (MQ=255)
                                                                                           tCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCcgc                                                  >  1:2151131/1‑148 (MQ=255)
                                                                                                      gTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGACAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGa                                       >  2:1827051/1‑148 (MQ=255)
                                                                                                       tATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGac                                      >  2:122004/1‑147 (MQ=255)
                                                                                                        aTGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGaaa                                     >  2:326213/1‑148 (MQ=255)
                                                                                                        aTGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGaaa                                     >  1:671725/1‑148 (MQ=255)
                                                                                                        aTGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGaaa                                     >  1:2957685/1‑148 (MQ=255)
                                                                                                             ggcgggcgGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAggg                                >  2:602029/1‑148 (MQ=255)
                                                                                                             ggcgggcgGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAggg                                >  2:601838/1‑148 (MQ=255)
                                                                                                             ggcgggcgGTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAggg                                <  1:772747/148‑1 (MQ=255)
                                                                                                              gcgggcggTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAgggg                               <  1:1243695/148‑1 (MQ=255)
                                                                                                              gcgggcggTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAgggg                               <  2:3522499/148‑1 (MQ=255)
                                                                                                                gggcggTAACTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTa                             <  1:1545824/148‑1 (MQ=255)
                                                                                                                       aaCTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATca                      >  1:2978409/1‑148 (MQ=255)
                                                                                                                       aaCTTCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATca                      <  1:3017097/148‑1 (MQ=255)
                                                                                                                           tCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATcaacaa                  >  2:3170781/1‑148 (MQ=255)
                                                                                                                           tCTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATcaacaa                  <  1:87943/148‑1 (MQ=255)
                                                                                                                            cTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACaaa                 <  2:3148138/148‑1 (MQ=255)
                                                                                                                            cTACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACaaa                 <  2:1719757/148‑1 (MQ=255)
                                                                                                                             tACCCTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAg                <  2:2694206/148‑1 (MQ=255)
                                                                                                                               cccTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCa              <  2:3314708/148‑1 (MQ=255)
                                                                                                                                ccTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGACAAAGGGGTAACCATCAACAAAGCAc             >  1:2694521/1‑148 (MQ=255)
                                                                                                                                ccTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCAc             >  1:570167/1‑148 (MQ=255)
                                                                                                                                ccTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCAc             >  1:2693434/1‑148 (MQ=255)
                                                                                                                                 cTTACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCAcc            >  2:834432/1‑148 (MQ=255)
                                                                                                                                   tACAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCgg          >  2:1729467/1‑148 (MQ=255)
                                                                                                                                     cAGTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGc        <  1:3111897/148‑1 (MQ=255)
                                                                                                                                       gTTGCAAGCCGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCga      >  2:2152534/1‑148 (MQ=255)
                                                                                                                                       gTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTACCCATCAACAAAGCACCGGGCga      >  1:2942162/1‑148 (MQ=255)
                                                                                                                                       gTTGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCga      <  1:3262325/148‑1 (MQ=255)
                                                                                                                                        ttGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCgat     >  1:1148/1‑148 (MQ=255)
                                                                                                                                        ttGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCgat     >  2:2369923/1‑148 (MQ=255)
                                                                                                                                         tGCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCgatg    <  2:176848/148‑1 (MQ=255)
                                                                                                                                          gCAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCgatga   <  1:2684260/148‑1 (MQ=255)
                                                                                                                                           cAAGCAGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCgatgat  >  1:3374172/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                              
ATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGCTGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGTAACTTCTACCCTTACAGTTGCAAGCCGGAAGATGTCTGGGCGGCGCTGGAGAAAGACCGGGAAAACCTGTTTTTTATCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGAT  >  CP009273/2834950‑2835236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑