Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,835,291 T→G 16.0% C315G (TGC→GGC)  ascB → cryptic 6‑phospho‑beta‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,835,2910TG16.0% 369.5 / 13.4 196C315G (TGC→GGC) ascBcryptic 6‑phospho‑beta‑glucosidase
Reads supporting (aligned to +/- strand):  ref base T (49/108);  new base G (35/2);  total (86/110)
Fisher's exact test for biased strand distribution p-value = 3.94e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 200 total aligned reads displayed.

TCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGTT  >  CP009273/2835145‑2835438
                                                                                                                                                  |                                                                                                                                                   
tCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTg                                                                                                                                                    <  1:190189/148‑1 (MQ=255)
tCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTg                                                                                                                                                    <  1:1631492/148‑1 (MQ=255)
 cGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTgc                                                                                                                                                   >  2:1157530/1‑148 (MQ=255)
   aTGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTTTCTCTTTCAGCTATTACGCCGCGCGCTgcgc                                                                                                                                                 >  1:1162047/1‑148 (MQ=255)
   aTGTGCAGGCGCGGGGCACGTATCCGGCGTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCCCCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTgcgc                                                                                                                                                 <  2:3016616/148‑1 (MQ=255)
    tgtgCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTGTCTTTGAGCTCTTTCGCCTCGCGCTGCGcc                                                                                                                                                >  2:400265/1‑148 (MQ=255)
      tgCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTTTGTCTTTCAGCGCTTACGCCCCGCGCGGCGccgc                                                                                                                                              >  1:671090/1‑146 (MQ=255)
       gCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTTTTTATTTCATCAATTTAGCCGCGCGGAGCGccccc                                                                                                                                             >  1:1700771/1‑145 (MQ=255)
       gCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTCTCTTTAAGCTATTACCCCACGCGCGGCGCCTcg                                                                                                                                             >  1:1700855/1‑148 (MQ=255)
             gcgGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAGGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGgag                                                                                                                                       <  1:982195/148‑1 (MQ=255)
             gcgGGGCACGTATCCGGCTTACTCTGCCCGCGTAGTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGgag                                                                                                                                       <  2:3547667/148‑1 (MQ=255)
               ggggCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGAt                                                                                                                                     <  2:1727271/148‑1 (MQ=255)
                 ggCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGa                                                                                                                                   <  1:573552/148‑1 (MQ=255)
                 ggCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGa                                                                                                                                   <  2:3219849/148‑1 (MQ=255)
                  gCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGaa                                                                                                                                  <  1:2056887/148‑1 (MQ=255)
                  gCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGaa                                                                                                                                  <  1:438334/148‑1 (MQ=255)
                       tATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTTTGGGTTTGAGCGATTACGCCGCGCGCGGCGCCGCGGCGGGGGTGAACGCca                                                                                                                             >  1:663492/1‑148 (MQ=255)
                       tATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTGTCTTTCAGCTATTAAGCCTCGCGCGGGGCCTCGGCGGGGATGTAAGCca                                                                                                                             >  1:2561176/1‑148 (MQ=255)
                       tATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCca                                                                                                                             <  2:3142248/148‑1 (MQ=255)
                            ggCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGTACGCCcacaac                                                                                                                        >  2:2742241/1‑148 (MQ=255)
                             gCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAacaaca                                                                                                                       <  1:594130/148‑1 (MQ=255)
                              cTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCGATTAAGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAAcag                                                                                                                      >  2:1860621/1‑148 (MQ=255)
                                 aCTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAAcagcag                                                                                                                   <  2:2150774/148‑1 (MQ=255)
                                 aCTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAAcagcag                                                                                                                   <  2:2150506/148‑1 (MQ=255)
                                    ctGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTGAGCTATTACGCCTCGCGCGGCGCCGCGGCGGCGATGAACGCCAACAACAGCAGTgc                                                                                                                >  2:621276/1‑148 (MQ=255)
                                        ccGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTTTGTATTTAAGCTATTAATCCTCCCGCGGCGCCGCGGCGGAGATTTACCCCAACAACAACACTgcggcg                                                                                                            >  1:3146127/1‑148 (MQ=255)
                                         cgcgTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGa                                                                                                           <  1:3371315/148‑1 (MQ=255)
                                         cgcgTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGa                                                                                                           <  2:3413668/148‑1 (MQ=255)
                                          gcgTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTTTCTCTTTAAGCTATTAACCCACGCGCTGCGCCACCGCGGAGAAGAACGCCAACAACACCAGTGCGGCGaa                                                                                                          >  1:573164/1‑148 (MQ=255)
                                           cgTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTTTCTCTTTAAGCTATTACGCCGCGCGCGGCCCCTCCGCGGTGATGTACGCCAACAACAGCAGTGCGGCGGAc                                                                                                         >  1:1450526/1‑148 (MQ=255)
                                              aTTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACgtg                                                                                                      <  2:1036725/148‑1 (MQ=255)
                                              aTTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTCTCTTTAACCTATTAAGCCTCCCGCGGCGCCGCGGCGGTGATGAAACCCAACAAAACCAGAGCGGCGAACgtg                                                                                                      >  1:485742/1‑148 (MQ=255)
                                                tCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTTTGTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACgtggt                                                                                                    >  2:3328216/1‑148 (MQ=255)
                                                tCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTCTCTTTCAGCTATTAAGCCTCGCGCGGCGCCTCGGCGGAGATGAAAGCCACCAACAGCAGGGCGGCGAACggggt                                                                                                    >  2:1565161/1‑148 (MQ=255)
                                                   gcgAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTAAGCTATTACGCCTCGCGCGGCGCCGCGGGGGAGATGAAAGCCAACAACAGCAGTGCGGCGAACGTGGTGaa                                                                                                 >  1:1784589/1‑148 (MQ=255)
                                                    cgAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGaaa                                                                                                <  1:787829/148‑1 (MQ=255)
                                                    cgAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGaaa                                                                                                <  2:7062/148‑1 (MQ=255)
                                                      aaaaaGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTCTCTTTGAGCTATTACGCCTCGCGCGGCGCCACGGCCGCGATGTACGCCAAAAACACCAGTGCGGCGAACGTGGGGAAATc                                                                                              >  2:1525241/1‑148 (MQ=255)
                                                       aaaaGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTTTATGTTTAAGCTATTACGCCACGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCCGTGCGGCGAACGTGGTGAAATCg                                                                                             >  2:1658386/1‑148 (MQ=255)
                                                        aaaGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGc                                                                                            <  2:2248596/148‑1 (MQ=255)
                                                         aaGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCt                                                                                           >  2:1446736/1‑148 (MQ=255)
                                                           ggggTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTGTCTTTTAGCTAATAAGCCGCGCGCGGCGGCGCGGCGGCGATGAAAGCCCACAACACCCGTGCGGCGCACCTGGTGGAATAGCGGc                                                                                         >  1:2467901/1‑148 (MQ=255)
                                                            gggTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCg                                                                                        <  1:601838/148‑1 (MQ=255)
                                                            gggTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCg                                                                                        >  2:849754/1‑148 (MQ=255)
                                                              gTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTa                                                                                      <  2:2151135/148‑1 (MQ=255)
                                                               tAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTaa                                                                                     <  1:3218982/148‑1 (MQ=255)
                                                               tAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTTTGTCTTTGAGCTATTAAGCCGCGCGCGGCGCCGCGGCGGAGATGTACGCCCACAACACCAGTGCGGCGCACCTGGTGACATCGCGGCGTaa                                                                                     >  1:3454748/1‑148 (MQ=255)
                                                                aaCCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTaaa                                                                                    >  2:508464/1‑147 (MQ=255)
                                                                 aCCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATc                                                                                   <  1:1715767/148‑1 (MQ=255)
                                                                    aTCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGt                                                                                <  2:3377273/148‑1 (MQ=255)
                                                                     tCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTTCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTa                                                                               <  2:1529310/148‑1 (MQ=255)
                                                                       aacacAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATc                                                                             <  2:2942162/148‑1 (MQ=255)
                                                                         caaAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTa                                                                           <  1:1157530/148‑1 (MQ=255)
                                                                          aaaGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTAc                                                                          <  1:1459927/148‑1 (MQ=255)
                                                                             gCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAgg                                                                       <  2:963/148‑1 (MQ=255)
                                                                              cACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGGGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGt                                                                      <  1:1729467/148‑1 (MQ=255)
                                                                                ccGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTGTCTTTCAGCTATTACGCCACGCGCTGCGCCGCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGa                                                                    >  2:1732284/1‑148 (MQ=255)
                                                                                ccGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGa                                                                    <  2:3146127/148‑1 (MQ=255)
                                                                                ccGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGa                                                                    <  2:1051035/148‑1 (MQ=255)
                                                                                ccGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGa                                                                    <  1:367683/148‑1 (MQ=255)
                                                                                    gCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGa                                                                <  2:2865158/148‑1 (MQ=255)
                                                                                       atgatgAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGActg                                                             <  2:2978409/148‑1 (MQ=255)
                                                                                        tgatgaAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGActgg                                                            <  2:2957685/148‑1 (MQ=255)
                                                                                         gatgaAATCCTGAAAAACACCGTCGATTTTGTCTCTTTGAGCTATTACGCCGCGCGCGGCGCCTCGGGGGGGATGTACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATAGCTGCGTAATCCGTATCTACAGGTGAGCGActggg                                                           >  1:2380837/1‑148 (MQ=255)
                                                                                          atgaAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGActgggg                                                          <  1:2369923/148‑1 (MQ=255)
                                                                                             aaaTCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGTAATCGCTGCGTAATCCGTATCTACCGGGGTGCGCctgggggtg                                                       >  2:3528093/1‑148 (MQ=255)
                                                                                              aaTCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGActggggctgg                                                      <  1:526402/148‑1 (MQ=255)
                                                                                                 ccTGAAAAACACCGTCGATTTTTTCTCTTTAAGCTATTAAGCCTCGCGCTGCGCCACGGCGGAGATTAACGCCAACAACACCAGTGCGGCGAACCTGGTGAAATCGCTGCGTAATCCGTATCTACACGTGAGCGACTGGGGCGGGGGa                                                   >  2:813227/1‑148 (MQ=255)
                                                                                                 ccTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGa                                                   <  1:873449/148‑1 (MQ=255)
                                                                                                  cTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGaa                                                  <  2:1419578/148‑1 (MQ=255)
                                                                                                    gacaaaCACCTTCGATTGTGTCTCTTTCAGCTATTACCCCTCGCGCGGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGGGAGCGACTGGGGCTGGGGAAtt                                                <  2:2694521/145‑1 (MQ=255)
                                                                                                    gAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAAtt                                                <  2:2693434/148‑1 (MQ=255)
                                                                                                     aaaaaCACCGTCGATTTTGTGTCTTTCAGCTATTACGCCTCGCGCTGCGCCACGGCGGAGATTAACGCCAACAACAGCACTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATATACAGGTGAGCGACGGGGGCTGGGGCATTg                                               >  2:1888776/1‑148 (MQ=255)
                                                                                                        aaCACCGTCGTTTTTGTCTCTTTCAGCTTTTACGCCTCGCGCGGCGCCTCGGCGTAGATGAACGCCAACAACAGAAGTGGGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATc                                            <  1:2730697/148‑1 (MQ=255)
                                                                                                          cacCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCa                                          <  2:3203867/148‑1 (MQ=255)
                                                                                                             cGTCGAGTTTGTGTATATCACCTATTAACCCAAGCGCAGCGCCGCCGCGGAGATGAAAGCCAACCACAGCACTGCGGCGAACGGGGTGTAAACCCGGCGGAAAACGTATCAAAACGTGAGCGACTGGGGCTGGGGAATTGATacacaa                                       >  2:1838368/1‑146 (MQ=255)
                                                                                                               tCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCgg                                     >  2:562548/1‑148 (MQ=255)
                                                                                                                  aTTTTGTCTCTTTCAGCTATTCCGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCt                                  <  2:3374172/148‑1 (MQ=255)
                                                                                                                  aTTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCt                                  <  2:1778090/148‑1 (MQ=255)
                                                                                                                   ttttGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTg                                 >  2:1371847/1‑148 (MQ=255)
                                                                                                                   ttttGTCTCTTACAGCTATTACGCCTCGCGCTGCGCCTCGGGGGAGATGAACGCCAACAACAGCAGGGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTg                                 <  1:326213/148‑1 (MQ=255)
                                                                                                                    tttGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGc                                >  1:2888742/1‑148 (MQ=255)
                                                                                                                         ctctTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATc                           >  1:1283692/1‑148 (MQ=255)
                                                                                                                          tctTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCa                          >  1:2644738/1‑148 (MQ=255)
                                                                                                                                aGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGGGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGa                    <  1:1380066/148‑1 (MQ=255)
                                                                                                                                 gCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGaa                   >  1:106231/1‑148 (MQ=255)
                                                                                                                                  cTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACCGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAat                  <  1:3170781/148‑1 (MQ=255)
                                                                                                                                   tATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAata                 <  1:1565161/148‑1 (MQ=255)
                                                                                                                                    aTTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAatat                >  1:1202197/1‑148 (MQ=255)
                                                                                                                                     ttccGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATatg               <  2:1700855/145‑1 (MQ=255)
                                                                                                                                     ttACGCCTCGCGCTGCGCCTCGGCGTAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGGGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATatg               <  2:1700771/148‑1 (MQ=255)
                                                                                                                                      tACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATatga              <  1:1829141/148‑1 (MQ=255)
                                                                                                                                       aCGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATatgat             >  2:2362914/1‑148 (MQ=255)
                                                                                                                                        cGCCTCGGGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATatgatg            <  1:1884188/148‑1 (MQ=255)
                                                                                                                                          ccTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTCTCACCATGAATatgcggta          >  2:1936373/1‑143 (MQ=255)
                                                                                                                                           cTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACCACAGCAGTGCGGCGAACGTGGTTAAATCGCTGCGTAATCCTTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTAc         <  1:2232852/148‑1 (MQ=255)
                                                                                                                                             cgcgCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGa       >  2:1089114/1‑148 (MQ=255)
                                                                                                                                             cgcgCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGa       <  1:1099004/148‑1 (MQ=255)
                                                                                                                                                  tGCGCGTCGGCGTAGATGAACTCCAACAACATCAGTGTGGCGAACTTGTTGAAATGGCTTGGTAATCCGTATATTATGGTGAGCGACTGGGGCTGGGGAATTGATCAACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGtt  <  2:1306416/148‑1 (MQ=255)
                                                                                                                                                  tGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGtt  <  2:1450526/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGTT  >  CP009273/2835145‑2835438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑