Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,879,449 G→T 100% S377* (TCA→TAA)  ygcB ← R‑loop helicase‑annealase Cas3 needed for Cascade anti‑viral activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,879,4490GT100.0% 821.3 / NA 243S377* (TCA→TAA) ygcBR‑loop helicase‑annealase Cas3 needed for Cascade anti‑viral activity
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (106/137);  total (106/137)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 243 total aligned reads displayed.

ATCAATCGTGCAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTGAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATTTGTTGATCAATAAGTTTCC  >  CP009273/2879304‑2879593
                                                                                                                                                 |                                                                                                                                                
aTCAATCGTGCAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTaa                                                                                                                                                >  2:2947642/1‑148 (MQ=255)
 tCAATCGTGCAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAAt                                                                                                                                               >  2:2784279/1‑148 (MQ=255)
   aaTCGTGCAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTg                                                                                                                                             <  2:1464980/148‑1 (MQ=255)
    aTCGTGCAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGc                                                                                                                                            <  1:3116002/148‑1 (MQ=255)
     tCGTGCAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGcc                                                                                                                                           <  1:2313621/148‑1 (MQ=255)
      cGTGCAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCa                                                                                                                                          >  2:149557/1‑148 (MQ=255)
        tGCAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATg                                                                                                                                        <  1:2645083/148‑1 (MQ=255)
            aaCGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCa                                                                                                                                    <  1:3209698/148‑1 (MQ=255)
            aaCGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGGGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCa                                                                                                                                    <  2:2439499/148‑1 (MQ=255)
              cGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAg                                                                                                                                  <  1:1468369/148‑1 (MQ=255)
               gCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGa                                                                                                                                 <  1:1568469/148‑1 (MQ=255)
                ccGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGaa                                                                                                                                <  2:2381087/148‑1 (MQ=255)
                  gATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATa                                                                                                                              <  1:1715126/148‑1 (MQ=255)
                  gATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATa                                                                                                                              >  2:884827/1‑148 (MQ=255)
                    tttGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAg                                                                                                                            <  1:3105378/148‑1 (MQ=255)
                     ttGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGa                                                                                                                           >  2:231797/1‑148 (MQ=255)
                          cAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTgg                                                                                                                      <  2:909022/148‑1 (MQ=255)
                           aaGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAACCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTggg                                                                                                                     >  2:2893968/1‑148 (MQ=255)
                            aGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTgggg                                                                                                                    <  1:3444662/148‑1 (MQ=255)
                            aGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTgggg                                                                                                                    >  1:2399008/1‑148 (MQ=255)
                            aGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTgggg                                                                                                                    <  1:3079602/148‑1 (MQ=255)
                             gAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGa                                                                                                                   >  2:1140084/1‑148 (MQ=255)
                              aaaCACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGAt                                                                                                                  <  2:584473/148‑1 (MQ=255)
                              aaaCACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGAt                                                                                                                  >  1:1527972/1‑148 (MQ=255)
                               aaCACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATg                                                                                                                 >  2:235785/1‑148 (MQ=255)
                                acacTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGa                                                                                                                >  2:1162893/1‑148 (MQ=255)
                                 cacTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGaa                                                                                                               >  2:3204118/1‑148 (MQ=255)
                                 cacTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGaa                                                                                                               <  2:1710974/148‑1 (MQ=255)
                                  acTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGaaa                                                                                                              <  2:439122/148‑1 (MQ=255)
                                  acTGTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGaaa                                                                                                              <  2:2180262/148‑1 (MQ=255)
                                   cTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGaaaa                                                                                                             <  2:1616568/148‑1 (MQ=255)
                                    tttCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAAt                                                                                                            <  1:1356394/148‑1 (MQ=255)
                                     ttCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATa                                                                                                           >  1:258261/1‑148 (MQ=255)
                                      tCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATaa                                                                                                          <  1:1907907/148‑1 (MQ=255)
                                       cTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCGTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAg                                                                                                         <  1:2030245/148‑1 (MQ=255)
                                        ttATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGt                                                                                                        <  2:1832914/148‑1 (MQ=255)
                                         tATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTg                                                                                                       <  1:3028807/148‑1 (MQ=255)
                                           ttGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGgc                                                                                                     >  2:2074398/1‑148 (MQ=255)
                                           ttGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGgc                                                                                                     >  1:3355156/1‑148 (MQ=255)
                                             gCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAATTGgctc                                                                                                   >  2:2537039/1‑148 (MQ=255)
                                                ttGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGgctcgcg                                                                                                >  2:2426750/1‑148 (MQ=255)
                                                ttGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGgctcgcg                                                                                                >  2:35788/1‑148 (MQ=255)
                                                 tgtgACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGgctcgcgc                                                                                               <  1:2240698/148‑1 (MQ=255)
                                                    gACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGgctcgcgctcg                                                                                            <  1:1109312/148‑1 (MQ=255)
                                                     aCAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGgctcgcgctcgc                                                                                           <  1:1229882/148‑1 (MQ=255)
                                                      cAACCCCTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGcgctcgct                                                                                          <  1:3203159/148‑1 (MQ=255)
                                                       aaCCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCtt                                                                                         <  2:1839013/148‑1 (MQ=255)
                                                            cTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCAtt                                                                                    <  1:2563125/148‑1 (MQ=255)
                                                            cTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCAtt                                                                                    <  1:1162893/148‑1 (MQ=255)
                                                            cTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCAtt                                                                                    <  1:555226/148‑1 (MQ=255)
                                                                  acacTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTa                                                                              <  2:2204433/148‑1 (MQ=255)
                                                                   cacTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTaa                                                                             >  2:1900492/1‑148 (MQ=255)
                                                                     cTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAagc                                                                           >  2:1507943/1‑148 (MQ=255)
                                                                        aaCCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAagcata                                                                        >  2:788441/1‑148 (MQ=255)
                                                                        aaCCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGGGAGTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCAGTCTCGTAagcata                                                                        >  1:2892074/1‑148 (MQ=255)
                                                                           ccACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAagcatagca                                                                     >  2:2913531/1‑148 (MQ=255)
                                                                               gCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTcg                                                                 >  1:2260784/1‑148 (MQ=255)
                                                                                 ttcttcttGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTcgcg                                                               <  1:3526783/148‑1 (MQ=255)
                                                                                      cttGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGc                                                          <  1:1790250/148‑1 (MQ=255)
                                                                                       ttGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCt                                                         <  2:141272/148‑1 (MQ=255)
                                                                                        tGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCtt                                                        >  2:2585397/1‑148 (MQ=255)
                                                                                           cccTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTtgtg                                                     <  1:457085/148‑1 (MQ=255)
                                                                                            ccTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTgtgt                                                    >  2:502914/1‑148 (MQ=255)
                                                                                               gTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTgg                                                 >  2:3453777/1‑148 (MQ=255)
                                                                                               gTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTgg                                                 <  2:3051984/148‑1 (MQ=255)
                                                                                                ttCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCACGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTggg                                                >  2:1930147/1‑148 (MQ=255)
                                                                                                   aGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAgg                                             >  1:2610018/1‑148 (MQ=255)
                                                                                                   aGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAgg                                             >  1:3389668/1‑148 (MQ=255)
                                                                                                    gTAATCGCGCGTGATTTTAGTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAggg                                            <  2:1979280/148‑1 (MQ=255)
                                                                                                        tCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCaaa                                        >  1:3240728/1‑148 (MQ=255)
                                                                                                         cgcgcgTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCaaaa                                       >  2:825450/1‑148 (MQ=255)
                                                                                                         cgcgcgTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCaaaa                                       >  2:2029208/1‑148 (MQ=255)
                                                                                                            gcgTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATa                                    <  2:244829/148‑1 (MQ=255)
                                                                                                              gTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAac                                  <  1:149557/148‑1 (MQ=255)
                                                                                                               tGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAaca                                 <  2:2387482/148‑1 (MQ=255)
                                                                                                                gATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAacac                                >  1:3298845/1‑148 (MQ=255)
                                                                                                                 aTTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACt                               >  2:68850/1‑148 (MQ=255)
                                                                                                                  ttttATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTa                              >  2:2966105/1‑148 (MQ=255)
                                                                                                                   tttATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTAt                             <  2:71506/148‑1 (MQ=255)
                                                                                                                   tttATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTAt                             >  1:679960/1‑148 (MQ=255)
                                                                                                                       ttgattgaAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGc                         >  1:1633926/1‑148 (MQ=255)
                                                                                                                        tgattgaAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCa                        <  2:2804103/148‑1 (MQ=255)
                                                                                                                         gattgaAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCaa                       <  1:1140084/148‑1 (MQ=255)
                                                                                                                         gattgaAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCaa                       <  2:2549857/148‑1 (MQ=255)
                                                                                                                         gattgaAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCaa                       <  2:1068906/148‑1 (MQ=255)
                                                                                                                          attgaAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAAt                      <  2:2886459/148‑1 (MQ=255)
                                                                                                                            tgaAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAAttt                    >  1:2693071/1‑148 (MQ=255)
                                                                                                                             gaAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATttg                   <  1:2759268/148‑1 (MQ=255)
                                                                                                                             gaAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATttg                   <  1:430557/148‑1 (MQ=255)
                                                                                                                                agagGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATttgttg                <  1:889846/148‑1 (MQ=255)
                                                                                                                                 gagGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGGTGGGAGGGCAAAAATAACACTATCCGCAATTTGTTGa               >  1:2311647/1‑148 (MQ=255)
                                                                                                                                  agGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATTTGTTGAt              <  1:497960/148‑1 (MQ=255)
                                                                                                                                  agGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTAGCCGCAATTTGTTGAt              >  2:2678727/1‑148 (MQ=255)
                                                                                                                                   ggtggtTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATTTGTTGATc             >  2:705619/1‑148 (MQ=255)
                                                                                                                                   ggtggtTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATTTGTTGATc             <  1:235810/148‑1 (MQ=255)
                                                                                                                                    gtggtTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATTTGTTGATCa            <  2:3274271/148‑1 (MQ=255)
                                                                                                                                         tAAACCGTTAATAGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATTTGTTGATCAATAAg       <  2:677755/148‑1 (MQ=255)
                                                                                                                                         tAAACCGTTAATAGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATTTGTTGATCAATAAg       <  2:1256132/148‑1 (MQ=255)
                                                                                                                                             ccGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATTTGTTGATCAATAAGTTTc   <  1:2947624/148‑1 (MQ=255)
                                                                                                                                             ccGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATTTGTTGATCAATAAGTTTc   <  1:231797/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ATCAATCGTGCAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTGAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATTTGTTGATCAATAAGTTTCC  >  CP009273/2879304‑2879593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑