Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,884,385 G→T 100% V291V (GTC→GTA)  cysJ ← sulfite reductase, alpha subunit, flavoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,884,3850GT97.7% 556.8 / 3.8 175V291V (GTC→GTA) cysJsulfite reductase, alpha subunit, flavoprotein
Reads supporting (aligned to +/- strand):  ref base G (0/4);  new base T (81/90);  total (81/94)
Fisher's exact test for biased strand distribution p-value = 1.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 176 total aligned reads displayed.

TGTTTCACTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCGACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTCTGGTTA  >  CP009273/2884238‑2884529
                                                                                                                                                   |                                                                                                                                                
tgtTTCACTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCg                                                                                                                                                  >  1:907058/1‑148 (MQ=255)
   ttCACTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACa                                                                                                                                               >  1:1269018/1‑148 (MQ=255)
     cACTGCGGGTAAGCGTGGCGTAATTCTCAACACTGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAg                                                                                                                                             <  1:3400953/148‑1 (MQ=255)
      aCTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGt                                                                                                                                            <  2:1405078/148‑1 (MQ=255)
       cTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGtt                                                                                                                                           >  2:967431/1‑148 (MQ=255)
       cTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGtt                                                                                                                                           >  2:438985/1‑148 (MQ=255)
        tGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCAAAGCAGTTCTACAAGTTc                                                                                                                                          >  2:3512795/1‑148 (MQ=255)
         gCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCt                                                                                                                                         <  2:2758018/148‑1 (MQ=255)
          cGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCtt                                                                                                                                        >  2:969416/1‑148 (MQ=255)
          cGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCtt                                                                                                                                        <  1:2096765/148‑1 (MQ=255)
          cGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCtt                                                                                                                                        <  1:299733/148‑1 (MQ=255)
            ggTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTc                                                                                                                                      >  2:1930617/1‑148 (MQ=255)
              tAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCAc                                                                                                                                    >  2:2065955/1‑148 (MQ=255)
               aaGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCAcc                                                                                                                                   >  2:2162701/1‑148 (MQ=255)
               aaGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCAcc                                                                                                                                   >  2:3070948/1‑148 (MQ=255)
               aaGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCAcc                                                                                                                                   >  2:374339/1‑148 (MQ=255)
                   gTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTg                                                                                                                               <  2:1767882/148‑1 (MQ=255)
                        gTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGa                                                                                                                          <  2:2590586/148‑1 (MQ=255)
                           aTTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCg                                                                                                                       <  1:374203/148‑1 (MQ=255)
                           aTTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCg                                                                                                                       <  1:374345/148‑1 (MQ=255)
                           aTTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCg                                                                                                                       >  1:2778710/1‑148 (MQ=255)
                            ttCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGt                                                                                                                      >  2:916229/1‑148 (MQ=255)
                                caacaaTGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTg                                                                                                                  <  1:2593807/148‑1 (MQ=255)
                                 aacaaTGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGa                                                                                                                 <  1:2886286/148‑1 (MQ=255)
                                  acaaTGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGAt                                                                                                                >  1:3410124/1‑148 (MQ=255)
                                  acaaTGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGAt                                                                                                                >  2:2730857/1‑148 (MQ=255)
                                  acaaTGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGAt                                                                                                                >  1:176330/1‑148 (MQ=255)
                                   caaTGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATa                                                                                                               >  1:2672550/1‑148 (MQ=255)
                                    aaTGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATAc                                                                                                              >  1:141313/1‑148 (MQ=255)
                                       gTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAg                                                                                                           <  2:547997/148‑1 (MQ=255)
                                        ttGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGa                                                                                                          <  1:1968663/148‑1 (MQ=255)
                                           gCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGc                                                                                                       >  1:2568312/1‑148 (MQ=255)
                                            cGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGcc                                                                                                      <  2:2936570/148‑1 (MQ=255)
                                              gtgtTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCa                                                                                                    >  1:1421758/1‑148 (MQ=255)
                                               tgtTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAg                                                                                                   >  2:2307425/1‑148 (MQ=255)
                                                        tCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCAcc                                                                                          >  1:594244/1‑148 (MQ=255)
                                                                aaGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGt                                                                                  >  2:511185/1‑148 (MQ=255)
                                                                   tGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAAc                                                                               >  2:3419318/1‑148 (MQ=255)
                                                                     ccACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGc                                                                             <  2:2330437/148‑1 (MQ=255)
                                                                      cACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCa                                                                            >  1:427131/1‑148 (MQ=255)
                                                                           tAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGccc                                                                       >  2:381279/1‑148 (MQ=255)
                                                                            aGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCg                                                                      >  2:2470319/1‑148 (MQ=255)
                                                                             gcgcTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGa                                                                     <  2:3311083/148‑1 (MQ=255)
                                                                                cTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTc                                                                  >  2:814282/1‑148 (MQ=255)
                                                                                 ttCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCa                                                                 >  1:1465330/1‑148 (MQ=255)
                                                                                     ttCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTa                                                             <  2:3487253/148‑1 (MQ=255)
                                                                                     ttCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCGACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTa                                                             <  1:3214083/148‑1 (MQ=255)
                                                                                      tCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTaa                                                            <  1:3124967/148‑1 (MQ=255)
                                                                                       cAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAg                                                           <  1:607749/148‑1 (MQ=255)
                                                                                          agGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCa                                                        >  1:1770869/1‑148 (MQ=255)
                                                                                          agGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCa                                                        <  2:207624/148‑1 (MQ=255)
                                                                                           ggCTACGTTTTGCCGTAGTCGGTGACTGGTTCATAGCATTTCAGCCACATCAGTTCTACAATTTCTTTAACCAGTGCCGGATAGTTCTGATACCAGACGCGCATCGATTCACCCGGCTGGTAACGCATTCCCGTGTCACCTAATTctt                                                       <  2:1328681/148‑3 (MQ=255)
                                                                                           ggCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCaa                                                       <  2:1328483/148‑1 (MQ=255)
                                                                                           ggCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCaa                                                       <  1:3316844/148‑1 (MQ=255)
                                                                                           ggCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCaa                                                       <  2:3100798/148‑1 (MQ=255)
                                                                                           ggCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCGACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCaa                                                       <  2:907058/148‑1 (MQ=255)
                                                                                            gCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAAt                                                      <  2:2340937/148‑1 (MQ=255)
                                                                                            gCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAAt                                                      >  2:688916/1‑148 (MQ=255)
                                                                                                cGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCg                                                  >  1:1584296/1‑148 (MQ=255)
                                                                                                 gTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCGACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGa                                                 <  2:1888291/148‑1 (MQ=255)
                                                                                                   tttGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGata                                               >  2:3238311/1‑148 (MQ=255)
                                                                                                   tttGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGata                                               >  2:3238319/1‑148 (MQ=255)
                                                                                                   tttGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGata                                               <  1:951146/148‑1 (MQ=255)
                                                                                                    ttGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGatat                                              <  2:1512750/148‑1 (MQ=255)
                                                                                                    ttGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGatat                                              >  1:1814896/1‑148 (MQ=255)
                                                                                                        ccTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCg                                          >  2:1875576/1‑148 (MQ=255)
                                                                                                             aCGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGt                                     <  1:140442/148‑1 (MQ=255)
                                                                                                             aCGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGt                                     <  1:1018396/148‑1 (MQ=255)
                                                                                                              cGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTc                                    <  2:1675625/148‑1 (MQ=255)
                                                                                                                ntGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCtt                                  >  1:2167800/2‑148 (MQ=255)
                                                                                                                  gACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCtttt                                <  2:806096/148‑1 (MQ=255)
                                                                                                                  gACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCtttt                                <  2:2586775/148‑1 (MQ=255)
                                                                                                                   aCAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCttttt                               <  2:983939/148‑1 (MQ=255)
                                                                                                                   aCAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCttttt                               <  2:1803560/148‑1 (MQ=255)
                                                                                                                     aGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCa                             <  1:1858599/148‑1 (MQ=255)
                                                                                                                      ggTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCag                            <  1:511185/148‑1 (MQ=255)
                                                                                                                         tCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGt                         >  2:2254752/1‑148 (MQ=255)
                                                                                                                         tCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGt                         >  2:799177/1‑148 (MQ=255)
                                                                                                                         tCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGt                         <  1:3129523/148‑1 (MQ=255)
                                                                                                                         tCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAAGTTCGATATGGCGAACGTCTTTTTCAGAGt                         >  2:854610/1‑148 (MQ=255)
                                                                                                                             cGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACg                     >  1:2234911/1‑148 (MQ=255)
                                                                                                                                cTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGccc                  <  2:2626874/148‑1 (MQ=255)
                                                                                                                                cTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGccc                  <  2:2032832/148‑1 (MQ=255)
                                                                                                                                 tttCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCg                 <  1:2180143/148‑1 (MQ=255)
                                                                                                                                 tttCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCg                 >  1:28362/1‑148 (MQ=255)
                                                                                                                                    cAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTa              <  1:944046/148‑1 (MQ=255)
                                                                                                                                      gCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAAt            >  1:3075775/1‑148 (MQ=255)
                                                                                                                                      gCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAAt            <  1:2711006/148‑1 (MQ=255)
                                                                                                                                       ccACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAAtt           <  2:176330/148‑1 (MQ=255)
                                                                                                                                       ccACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAAtt           >  1:999266/1‑148 (MQ=255)
                                                                                                                                       ccACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAAtt           >  2:1180146/1‑148 (MQ=255)
                                                                                                                                         acaGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAAtttt         <  1:734405/148‑1 (MQ=255)
                                                                                                                                         acaGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAAtttt         <  1:2518778/148‑1 (MQ=255)
                                                                                                                                         acaGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAAtttt         <  2:1017450/148‑1 (MQ=255)
                                                                                                                                         acaGCAGTTCGACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAAtttt         <  1:2054798/148‑1 (MQ=255)
                                                                                                                                            gcagTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTCTg      >  1:120248/1‑148 (MQ=255)
                                                                                                                                             cagTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTCTgg     >  1:437300/1‑148 (MQ=255)
                                                                                                                                                ttCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTCTGGTTa  <  1:202742/148‑1 (MQ=255)
                                                                                                                                                ttCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTCTGGTTa  <  1:640050/148‑1 (MQ=255)
                                                                                                                                                ttCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTCTGGTTa  <  2:2933446/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TGTTTCACTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCGACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTCTGGTTA  >  CP009273/2884238‑2884529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑