Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,915,998 C→A 100% A116S (GCT→TCT)  yqcA ← short‑chain flavodoxin, FMN‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,915,9980CA97.5% 644.8 / 6.4 203A116S (GCT→TCT) yqcAshort‑chain flavodoxin, FMN‑binding
Reads supporting (aligned to +/- strand):  ref base C (4/1);  new base A (100/98);  total (104/99)
Fisher's exact test for biased strand distribution p-value = 3.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 203 total aligned reads displayed.

TCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGCGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGAAAGAGTGGCACA  >  CP009273/2915854‑2916140
                                                                                                                                                |                                                                                                                                              
tcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGct                                                                                                                                             >  2:423263/1‑148 (MQ=255)
      ccGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTc                                                                                                                                       <  1:2542601/148‑1 (MQ=255)
      ccGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTc                                                                                                                                       <  1:764084/148‑1 (MQ=255)
      ccGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTc                                                                                                                                       >  2:3371459/1‑148 (MQ=255)
       cGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTcc                                                                                                                                      <  2:2286651/148‑1 (MQ=255)
          tAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGc                                                                                                                                   >  1:1824108/1‑148 (MQ=255)
            aaaCGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGcaa                                                                                                                                 >  1:1651508/1‑148 (MQ=255)
            aaaCGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGcaa                                                                                                                                 >  2:2888880/1‑148 (MQ=255)
            aaaCGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGcaa                                                                                                                                 >  1:117845/1‑148 (MQ=255)
              aCGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCTGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGcaaca                                                                                                                               >  1:1925729/1‑148 (MQ=255)
              aCGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCTGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGcaaca                                                                                                                               >  1:128314/1‑148 (MQ=255)
              aCGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGcaaca                                                                                                                               >  2:509999/1‑148 (MQ=255)
              aCGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGcaaca                                                                                                                               >  2:2966255/1‑148 (MQ=255)
                gACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAg                                                                                                                             <  1:2980582/148‑1 (MQ=255)
                gACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAg                                                                                                                             <  2:1528540/148‑1 (MQ=255)
                  cATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGCGCTCTGTTCCTGCAACAAGGc                                                                                                                           <  2:1325663/148‑1 (MQ=255)
                    tttACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCAt                                                                                                                         <  1:2701501/148‑1 (MQ=255)
                         cgctcgctGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAAc                                                                                                                    >  1:1360209/1‑148 (MQ=255)
                         cgctcgctGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAAc                                                                                                                    <  1:1020204/148‑1 (MQ=255)
                            tcgctGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGCGCTCTGTTCCTGCAACAAGGCATCGAACTGt                                                                                                                 >  1:3296425/1‑148 (MQ=255)
                            tcgctGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGt                                                                                                                 <  2:2653541/148‑1 (MQ=255)
                            tcgctGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGt                                                                                                                 >  2:162142/1‑148 (MQ=255)
                            tcgctGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGt                                                                                                                 >  2:3144114/1‑148 (MQ=255)
                                   aCATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTgccgc                                                                                                          >  2:2944715/1‑148 (MQ=255)
                                    cATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTgtcgcc                                                                                                         >  1:1885842/1‑148 (MQ=255)
                                    cATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTgccgcc                                                                                                         >  2:2153423/1‑148 (MQ=255)
                                     atatCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCa                                                                                                        <  1:608727/148‑1 (MQ=255)
                                       atCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCAtt                                                                                                      >  1:3286924/1‑148 (MQ=255)
                                         cAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGc                                                                                                    >  2:2809483/1‑148 (MQ=255)
                                          aGGACCTCAGCGTGCCCCTCTGTTCGACCCACGGTTTTGATTCCGTTTCCGGGTCCGGGTTCTCACTGGCGTCAATCAACAGCAGTTCGCCAACCCGCTGAGAGCTCTGGTCCTGCACCAAGTCATCGAACTGTTTGCCGCCATTGCa                                                                                                   <  2:1931137/148‑1 (MQ=255)
                                            gACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGa                                                                                                 <  1:2847517/148‑1 (MQ=255)
                                             acaacaGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGaa                                                                                                <  2:143447/148‑1 (MQ=255)
                                             acaacaGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGaa                                                                                                <  2:903606/148‑1 (MQ=255)
                                             acaacaGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGaa                                                                                                <  2:143370/148‑1 (MQ=255)
                                             acaacaGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGaa                                                                                                >  1:392698/1‑148 (MQ=255)
                                              caacaGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGaaa                                                                                               <  1:2738073/148‑1 (MQ=255)
                                              caacaGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGaaa                                                                                               <  1:961068/148‑1 (MQ=255)
                                                 caGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTc                                                                                            <  1:1501478/148‑1 (MQ=255)
                                                   gCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTcac                                                                                          >  1:2169427/1‑148 (MQ=255)
                                                    cGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTcaca                                                                                         >  2:1059197/1‑148 (MQ=255)
                                                    cGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTcaca                                                                                         >  2:1022649/1‑148 (MQ=255)
                                                      tGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATa                                                                                       <  1:3027307/148‑1 (MQ=255)
                                                      tGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATa                                                                                       <  1:999200/148‑1 (MQ=255)
                                                        ccccACTGTTCGCCCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAac                                                                                     <  1:2222658/148‑1 (MQ=255)
                                                        ccccACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAac                                                                                     >  1:3390136/1‑148 (MQ=255)
                                                         cccACTGGTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAact                                                                                    <  2:3118107/148‑1 (MQ=255)
                                                          ccACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAacta                                                                                   <  1:908879/148‑1 (MQ=255)
                                                           cACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAactac                                                                                  >  2:771031/1‑148 (MQ=255)
                                                           cACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAactac                                                                                  <  1:1823038/148‑1 (MQ=255)
                                                           cACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAactac                                                                                  >  2:3385839/1‑148 (MQ=255)
                                                             cTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTg                                                                                <  2:2708646/148‑1 (MQ=255)
                                                              tGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGt                                                                               >  1:26605/1‑148 (MQ=255)
                                                               gTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTc                                                                              >  1:2037683/1‑148 (MQ=255)
                                                                     cccACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAg                                                                        >  2:223462/1‑148 (MQ=255)
                                                                     cccACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAg                                                                        <  2:3279030/148‑1 (MQ=255)
                                                                           gATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAAt                                                                  <  2:2093949/148‑1 (MQ=255)
                                                                             tttGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCa                                                                >  1:377641/1‑148 (MQ=255)
                                                                              ttGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGCGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCAc                                                               >  1:2766604/1‑148 (MQ=255)
                                                                              ttGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGCGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCAc                                                               >  1:2120821/1‑148 (MQ=255)
                                                                               tGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACg                                                              <  1:423263/148‑1 (MQ=255)
                                                                               tGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACg                                                              >  2:439216/1‑148 (MQ=255)
                                                                                 aTTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGcc                                                            <  2:3223420/148‑1 (MQ=255)
                                                                                   tCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCAt                                                          <  2:1751771/148‑1 (MQ=255)
                                                                                   tCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCAt                                                          >  2:3392445/1‑148 (MQ=255)
                                                                                    ccGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATa                                                         <  2:3486887/148‑1 (MQ=255)
                                                                                    ccGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATa                                                         >  2:1446752/1‑148 (MQ=255)
                                                                                     cGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATaa                                                        <  2:3313204/148‑1 (MQ=255)
                                                                                     cGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATaa                                                        <  2:117845/148‑1 (MQ=255)
                                                                                       tttCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACg                                                      <  1:323458/148‑1 (MQ=255)
                                                                                        ttccggttccggGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGc                                                     <  1:3542849/148‑1 (MQ=255)
                                                                                          ccggttccggGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAg                                                   >  1:2868815/1‑148 (MQ=255)
                                                                                            ggttccggGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGAt                                                 <  1:1280090/148‑1 (MQ=255)
                                                                                               tccggGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCg                                              <  1:2897378/148‑1 (MQ=255)
                                                                                               tccggGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCg                                              >  2:662897/1‑148 (MQ=255)
                                                                                               tccggGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCg                                              >  2:1778595/1‑148 (MQ=255)
                                                                                                ccggGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCgg                                             <  1:226004/148‑1 (MQ=255)
                                                                                                   ggTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTg                                          >  1:1808297/1‑148 (MQ=255)
                                                                                                      tctcACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGaa                                       >  1:1726968/1‑148 (MQ=255)
                                                                                                      tctcACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGaa                                       >  1:2025996/1‑148 (MQ=255)
                                                                                                        tcACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGc                                     >  2:2400366/1‑148 (MQ=255)
                                                                                                         cACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGTCTGGAAGcc                                    <  1:3226126/148‑1 (MQ=255)
                                                                                                          aCTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGccc                                   <  2:529047/148‑1 (MQ=255)
                                                                                                            tGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAg                                 >  2:2769407/1‑148 (MQ=255)
                                                                                                             ggCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGa                                <  2:355187/148‑1 (MQ=255)
                                                                                                              gCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTACAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGAc                               >  1:2592303/1‑148 (MQ=255)
                                                                                                                 tcaatcaaCAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTAt                            >  1:2482716/1‑148 (MQ=255)
                                                                                                                 tcaatcaaCAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTAt                            >  1:3287590/1‑148 (MQ=255)
                                                                                                                  caatcaaCAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATc                           <  2:221976/148‑1 (MQ=255)
                                                                                                                  caatcaaCAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATc                           <  1:3385655/148‑1 (MQ=255)
                                                                                                                  caatcaaCAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATc                           >  2:2203203/1‑148 (MQ=255)
                                                                                                                   aatcaaCAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCt                          <  2:3119555/148‑1 (MQ=255)
                                                                                                                     tcaaCAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCttt                        <  1:3084683/148‑1 (MQ=255)
                                                                                                                         cAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGAtt                    >  1:836380/1‑148 (MQ=255)
                                                                                                                              tttCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTg               >  1:2860934/1‑148 (MQ=255)
                                                                                                                                tCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGaa             <  1:28135/148‑1 (MQ=255)
                                                                                                                                    cAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGTCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGAAagag         <  2:1885842/148‑1 (MQ=255)
                                                                                                                                    cAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGAAagag         <  1:2696357/148‑1 (MQ=255)
                                                                                                                                     aaCCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGAAAGAGt        >  2:2338951/1‑148 (MQ=255)
                                                                                                                                      aCCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGAAAGAGTg       >  2:1966479/1‑148 (MQ=255)
                                                                                                                                           cTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGAAAGAGTGGcaca  <  1:1611970/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                              
TCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGCGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGAAAGAGTGGCACA  >  CP009273/2915854‑2916140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑