BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,918,415	G→A	94.0%	P75P (CCC→CCT)	syd ←	SecY‑interacting protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,918,415	0	G	A	94.0%	696.5 / 28.2	235	P75P (CCC→CCT)	syd	SecY‑interacting protein
Reads supporting (aligned to +/- strand): ref base G (11/3); new base A (108/111); total (121/114)
Fisher's exact test for biased strand distribution p-value = 5.09e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 237 total aligned reads displayed.

AGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGC  >  CP009273/2918274‑2918559

aGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGt                                                                                                                                            <  1:3032193/148‑1 (MQ=255)

 gTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTa                                                                                                                                           <  2:2184229/148‑1 (MQ=255)

         aTGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAata                                                                                                                                   <  2:1316820/148‑1 (MQ=255)

         aTGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAata                                                                                                                                   <  2:1888949/148‑1 (MQ=255)

         aTGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAata                                                                                                                                   <  1:1674480/148‑1 (MQ=255)

          tGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCACTGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGACTTGTAGGTTGTATCACAatat                                                                                                                                  >  2:3008586/1‑148 (MQ=255)

             ccAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCaa                                                                                                                               >  1:2414299/1‑148 (MQ=255)

              cAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCaaa                                                                                                                              <  1:973683/148‑1 (MQ=255)

                 tCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAgc                                                                                                                           <  2:137711/148‑1 (MQ=255)

                    gATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGcgcg                                                                                                                        <  1:195877/148‑1 (MQ=255)

                    gATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGcgcg                                                                                                                        <  1:2445978/148‑1 (MQ=255)

                       tttCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTc                                                                                                                     >  1:2792674/1‑148 (MQ=255)

                        ttCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCa                                                                                                                    <  2:1848690/148‑1 (MQ=255)

                         tCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCaa                                                                                                                   <  1:2590233/148‑1 (MQ=255)

                              cACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACcgcg                                                                                                              <  1:200207/148‑1 (MQ=255)

                              cACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACcgcg                                                                                                              <  1:230469/148‑1 (MQ=255)

                                cccGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGtt                                                                                                            >  1:682067/1‑148 (MQ=255)

                                cccGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGtt                                                                                                            >  1:1586291/1‑148 (MQ=255)

                                cccGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGtt                                                                                                            >  1:1586125/1‑148 (MQ=255)

                                 ccGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGttt                                                                                                           <  1:3280816/148‑1 (MQ=255)

                                 ccGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGttt                                                                                                           <  1:3280792/148‑1 (MQ=255)

                                 ccGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGttt                                                                                                           >  1:2393613/1‑148 (MQ=255)

                                 ccGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGttt                                                                                                           >  2:2856609/1‑148 (MQ=255)

                                 ccGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGttt                                                                                                           <  2:3197970/148‑1 (MQ=255)

                                 ccGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGttt                                                                                                           <  2:3277326/148‑1 (MQ=255)

                                   gACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTAc                                                                                                         >  2:1830128/1‑148 (MQ=255)

                                     cGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACAt                                                                                                       <  1:107368/148‑1 (MQ=255)

                                      ggAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACAtt                                                                                                      <  1:2927297/148‑1 (MQ=255)

                                        aaGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACAtttt                                                                                                    >  1:272644/1‑148 (MQ=255)

                                         aGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTg                                                                                                   >  1:1908628/1‑148 (MQ=255)

                                          gtcgtcTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGt                                                                                                  <  2:2748553/148‑1 (MQ=255)

                                             gtcTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTcc                                                                                               <  2:206958/148‑1 (MQ=255)

                                             gtcTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTcc                                                                                               <  2:207521/148‑1 (MQ=255)

                                                    cTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTaa                                                                                        <  1:1216757/148‑1 (MQ=255)

                                                    cTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTaa                                                                                        >  2:2554847/1‑148 (MQ=255)

                                                      ccAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAAc                                                                                      <  2:800342/148‑1 (MQ=255)

                                                        aGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACgg                                                                                    <  2:1860228/148‑1 (MQ=255)

                                                        aGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACgg                                                                                    >  1:3065323/1‑148 (MQ=255)

                                                         ggTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGc                                                                                   >  2:2036575/1‑148 (MQ=255)

                                                         ggTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGc                                                                                   >  2:1657705/1‑148 (MQ=255)

                                                           tttGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTc                                                                                                <  2:2395585/133‑1 (MQ=255)

                                                           tttGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTg                                                                                 >  2:3539869/1‑148 (MQ=255)

                                                            ttGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTgg                                                                                <  2:3305568/148‑1 (MQ=255)

                                                               cagcaATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGt                                                                             >  1:730560/1‑148 (MQ=255)

                                                               cagcaATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGt                                                                             >  1:1059734/1‑148 (MQ=255)

                                                                  caATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGc                                                                          >  1:2725015/1‑148 (MQ=255)

                                                                  caATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCAGGCAAACTGAGTCGTATAAAATGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGAGTTTACATTTTGATACCCGGTAAACGGCTGGGGTTGc                                                                          >  1:186383/1‑148 (MQ=255)

                                                                    aTGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCa                                                                        >  2:2871611/1‑148 (MQ=255)

                                                                     tGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAg                                                                       >  1:2956240/1‑148 (MQ=255)

                                                                      gTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAACCTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTACAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGt                                                                      <  1:889784/148‑1 (MQ=255)

                                                                      gTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGt                                                                      >  1:637393/1‑148 (MQ=255)

                                                                      gTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATGGTAGGTTTTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTACACGGCTGGGGTTGCCAGt                                                                      <  2:1532529/148‑1 (MQ=255)

                                                                        cAGTTTGATATCGCCTACCTTCGCGTGCATATCCCCGGCAAACTGAGTCGTATACTTGGTATGAATTGTAGGTTGTATCACAATTTCAAAAGCGCGTTCAACCGCGTTTACTTTTTTTTCCCCGGTAACCGGCTGGGGTTTCCatttt                                                                    <  2:1692365/148‑5 (MQ=255)

                                                                           tttGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACg                                                                 >  2:771448/1‑148 (MQ=255)

                                                                            ttGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACgg                                                                <  1:2166545/148‑1 (MQ=255)

                                                                             tGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAACGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGc                                                               <  1:3013778/148‑1 (MQ=255)

                                                                               atatCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCAt                                                             <  2:372567/148‑1 (MQ=255)

                                                                               atatCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTAAAAACGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTCTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCAt                                                             <  2:372516/148‑1 (MQ=255)

                                                                                tatCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATc                                                            >  1:99919/1‑148 (MQ=255)

                                                                                tatCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATc                                                            >  1:204775/1‑148 (MQ=255)

                                                                                tatCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATc                                                            <  2:2369521/148‑1 (MQ=255)

                                                                                 atCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTCTAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCt                                                           >  1:1528545/1‑148 (MQ=255)

                                                                                     ccAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCgg                                                       >  1:2368647/1‑148 (MQ=255)

                                                                                       aaaCTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATTAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTa                                                     <  2:2414299/148‑1 (MQ=255)

                                                                                       aaaCTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTa                                                     <  2:625038/148‑1 (MQ=255)

                                                                                         aCTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGt                                                   >  1:598532/1‑148 (MQ=255)

                                                                                            gcgcGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGa                                                >  2:2617691/1‑148 (MQ=255)

                                                                                             cgcgTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGaa                                               >  2:2253094/1‑148 (MQ=255)

                                                                                              gcgTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGaaa                                              >  2:1378309/1‑148 (MQ=255)

                                                                                               cgTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAaat                                             >  1:1779576/1‑148 (MQ=255)

                                                                                               cgTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAaat                                             >  1:1329763/1‑148 (MQ=255)

                                                                                                  gCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAaataat                                          >  1:467434/1‑148 (MQ=255)

                                                                                                    atatCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGc                                        <  1:2474614/148‑1 (MQ=255)

                                                                                                      atCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCAc                                      >  1:516571/1‑148 (MQ=255)

                                                                                                          ccGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTg                                  >  1:2544792/1‑148 (MQ=255)

                                                                                                           cGGCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGa                                 <  2:911538/148‑1 (MQ=255)

                                                                                                            ggCAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGaa                                >  1:3456897/1‑148 (MQ=255)

                                                                                                              cAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAgg                              <  1:2776414/148‑1 (MQ=255)

                                                                                                              cAAACTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAgg                              >  2:2764754/1‑148 (MQ=255)

                                                                                                                aaCTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGaa                            >  1:1684676/1‑148 (MQ=255)

                                                                                                                 aCTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAac                           <  1:3539869/148‑1 (MQ=255)

                                                                                                                 aCTGAGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAac                           <  2:2998580/148‑1 (MQ=255)

                                                                                                                     aGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCg                       >  1:3134499/1‑148 (MQ=255)

                                                                                                                     aGTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCg                       <  2:3350841/148‑1 (MQ=255)

                                                                                                                      gTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGt                      <  2:119602/148‑1 (MQ=255)

                                                                                                                      gTCGTATAAAAGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGt                      <  1:3530183/148‑1 (MQ=255)

                                                                                                                        cGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTAc                    <  1:2871613/148‑1 (MQ=255)

                                                                                                                          tataAAAGGTCTGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAg                  <  2:693918/148‑1 (MQ=255)

                                                                                                                               aaGGTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCt             >  2:2711740/1‑148 (MQ=255)

                                                                                                                                 ggTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCa           >  1:335886/1‑148 (MQ=255)

                                                                                                                                 ggTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCa           <  1:1038129/148‑1 (MQ=255)

                                                                                                                                 ggTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTAAATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCa           <  1:148409/148‑1 (MQ=255)

                                                                                                                                 ggTATGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACAGCGTATACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTATTTGAAAAAATGCACGGTGAAGGAACACCGTACAGTTCCtaa           >  2:3078563/1‑146 (MQ=255)

                                                                                                                                    aTGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACtt        >  2:1184972/1‑148 (MQ=255)

                                                                                                                                    aTGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACtt        >  1:2605122/1‑148 (MQ=255)

                                                                                                                                     tGAATTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTa       <  2:2651841/148‑1 (MQ=255)

                                                                                                                                        aTTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACg    >  2:1859582/1‑148 (MQ=255)

                                                                                                                                        aTTGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACg    >  2:934015/1‑148 (MQ=255)

                                                                                                                                         ttGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACgg   >  1:557771/1‑148 (MQ=255)

                                                                                                                                         ttGTAGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACgg   >  1:2128145/1‑148 (MQ=255)

AGTCACCAGATGACCAATCAGATTTTCCTGCACCCGACGGAAGTCGTCTTCACTCCAGGTTTGCAGCAATGTCAGTTTGATATCGCCAAACTGCGCGTGCATATCCCCGGCAAACTGAGTCGTATAAAAGGTATGAATTGTGGGTTGTATCACAATATCAAAAGCGCGTTCAACCGCGTTTACATTTTGTTCCCCGGTAAACGGCTGGGGTTGCCAGTATACGGCATCTTCGGTAGTTGAAATAATGCACGGTGAAGGAACACCGTACAGTTCCTCACTTAACGGC  >  CP009273/2918274‑2918559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`