Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,940,854 G→T 8.4% intergenic (+32/‑2) metZ → / → metW tRNA‑Met/tRNA‑Met

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,940,8540GT 8.4% 352.4 / 10.0 114intergenic (+32/‑2)metZ/metWtRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base G (45/58);  new base T (4/7);  total (49/65)
Fisher's exact test for biased strand distribution p-value = 7.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

GCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGC  >  CP009273/2940707‑2940998
                                                                                                                                                    |                                                                                                                                                
gCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTg                                                                                                                                                  <  1:1123803/148‑1 (MQ=255)
gCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTg                                                                                                                                                  <  1:967686/148‑1 (MQ=255)
gCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTg                                                                                                                                                  <  1:967675/148‑1 (MQ=255)
 cATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGTTTCAAATCCGGACCCCGCAACAAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGa                                                                                                                                                 >  1:1095236/1‑148 (MQ=255)
  aTGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGAc                                                                                                                                                >  1:115558/1‑148 (MQ=255)
     aaaaCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAATCTTATAGC‑AATTGAGGCAGTAAGGTGAcgcg                                                                                                                                             >  2:503121/1‑148 (MQ=18)
        aCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCgggg                                                                                                                                          >  1:431717/1‑148 (MQ=255)
         cgcgAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCTGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAATTATGAGG‑AAGAAAAGCAGTAATGTGACGAGGGGt                                                                                                                                         >  1:1023407/1‑148 (MQ=17)
             aGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAACATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGag                                                                                                                                     <  2:110551/148‑1 (MQ=255)
             aGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAATTATATG‑AACAAAAGAGGAAAGGTGACGCGTGGtctag                                                                                                                                     >  1:1378910/1‑144 (MQ=17)
               cGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGagca                                                                                                                                   <  2:1335074/148‑1 (MQ=255)
               cGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGagca                                                                                                                                   <  2:3069000/148‑1 (MQ=255)
                 gAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGagcagc                                                                                                                                 <  2:1352139/148‑1 (MQ=255)
                 gAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGagcagc                                                                                                                                 <  2:2363250/148‑1 (MQ=255)
                  aGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGcc                                                                                                                                <  1:2928660/148‑1 (MQ=255)
                    tataGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTg                                                                                                                              >  1:1180746/1‑148 (MQ=255)
                      taGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATT‑AAATTTGAAGCAATAAAAGCAGTACGGTGACGAGGGCTGCATCAGACTTGt                                                                                                                            >  1:3245421/1‑148 (MQ=11)
                       aGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTa                                                                                                                           <  1:3547001/148‑1 (MQ=255)
                         tGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGc                                                                                                                         <  1:492842/148‑1 (MQ=255)
                          gcgcATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCt                                                                                                                        <  1:3431783/148‑1 (MQ=255)
                              aTCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTTACGCGGGGTGGAGCAGCCTGGTAGCtcgtc                                                                                                                    >  1:3433299/1‑148 (MQ=255)
                               tCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtcgtcg                                                                                                                   <  2:252173/148‑1 (MQ=255)
                                    ggACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTc                                                                                                              <  1:1582683/148‑1 (MQ=255)
                                             gTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTCAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGa                                                                                                     <  1:2612320/148‑1 (MQ=25)
                                             gTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGa                                                                                                     <  1:2066951/148‑1 (MQ=40)
                                              tGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGaa                                                                                                    <  1:155762/148‑1 (MQ=40)
                                              tGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGaa                                                                                                    <  1:1305315/148‑1 (MQ=40)
                                                gAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAgg                                                                                                  <  2:194662/148‑1 (MQ=40)
                                                catcagcCTGGTAGCTCGTCGGGCAAATAACCCGAAGGACGTCGGGTCAAATCCCGACCCCGCAACCAAATAAAATTTGAAG‑AAGTAAACCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAgg                                                                                                  <  2:1783279/145‑1 (MQ=11)
                                                   cagcCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTTACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcg                                                                                               <  2:3433299/148‑1 (MQ=255)
                                                   cagcCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcg                                                                                               <  1:1140664/148‑1 (MQ=40)
                                                   cagcCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcg                                                                                               <  1:1393508/148‑1 (MQ=40)
                                                    agcCTGGTATCTCGTCTGGCTCATAACCCTAAGGTCGTCGGTTCTAATCCCGCCCCCGCAAAGAATTAGATTTTGATG‑AAGTAATGCCTTACGGTTACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcgt                                                                                              <  2:865899/148‑1 (MQ=11)
                                                    agcCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAACATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcgt                                                                                              <  1:422998/148‑1 (MQ=38)
                                                      ccTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGtcgtcg                                                                                            >  1:506384/1‑148 (MQ=40)
                                                        ttgTAGCTCGTCGGGCTCATAAGCCGAAGGTCGTCGGTTCATATCCGGCCCCCGCAACCAATTAAACTTTGATG‑AAGAAAAGGAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGt                                                                                          <  1:1135614/146‑1 (MQ=11)
                                                         ggTAGCTCGTCGGGCACATAACCCGAAGGTCGTCGGTTCAAATCCTGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGtt                                                                                         <  2:100915/148‑1 (MQ=255)
                                                            aGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCaa                                                                                      <  1:2889250/148‑1 (MQ=40)
                                                              cTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAt                                                                                    >  1:3314306/1‑148 (MQ=40)
                                                                cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCAGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATcc                                                                                  <  2:1595686/148‑1 (MQ=255)
                                                                          agaaCCCGAAGGTCTTCTCTTCAAATCAGCCCCCCTCCACCAATTAAAATTTGATG‑AAGTAACGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCa                                                                        <  2:2507635/146‑1 (MQ=14)
                                                                              gcagAAGGTCTTCGGTTCTTATACGTACCGGGCCACCAATTAAAATTTGATG‑AAGTATTGCAGTCCGGTTTCGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCTTAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCa                                                                    <  2:1859409/145‑1 (MQ=14)
                                                                              cccGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAATTTGGATG‑AAGTTAAGCAGTACGGGTACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCa                                                                    <  2:1858504/148‑1 (MQ=11)
                                                                              cccGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTATAGCAGTACGGTTACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCa                                                                    <  2:1038920/148‑1 (MQ=255)
                                                                              cccGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCa                                                                    <  1:1636709/148‑1 (MQ=40)
                                                                               ccGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTCAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCaa                                                                   <  1:595408/148‑1 (MQ=25)
                                                                               ccGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTACAATGTGATG‑AAGTAACGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCaa                                                                   <  1:595384/148‑1 (MQ=255)
                                                                               ccGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAAATTGATG‑AAGAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCaa                                                                   <  1:595337/148‑1 (MQ=255)
                                                                                  aaGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCa                                                                >  1:425565/1‑148 (MQ=255)
                                                                                   aGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTTACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCaa                                                               >  2:37398/1‑148 (MQ=255)
                                                                                    ggTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAGCaaa                                                              >  1:2444433/1‑148 (MQ=255)
                                                                                    ggTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGAACCCAATCaaa                                                              >  1:2444473/1‑148 (MQ=255)
                                                                                     gtggtcgGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGTTG‑TAGTTAAGGAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAt                                                             <  2:513084/145‑1 (MQ=14)
                                                                                     gtcttcGGTTCAAATCCTTACCCCGCAACCAAATAAACTGCGCAG‑AAGAAACGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAt                                                             <  1:433474/148‑1 (MQ=14)
                                                                                     gtcgtcGGTTGATATCCGGCCCCGTCAACCAATTAAAATTTGATG‑TAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAt                                                             <  2:513090/148‑1 (MQ=14)
                                                                                     gtcgtcGGTTCAAATCCGGCCCGCGCAACCAATTATAATTTGTTG‑AAGTAACGGAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAt                                                             <  2:513085/148‑1 (MQ=14)
                                                                                     gtcgtcGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCACCCCATCAAAt                                                             >  2:2644273/1‑148 (MQ=255)
                                                                                     gtcgtcGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAt                                                             >  1:2152946/1‑148 (MQ=255)
                                                                                      tcgtcgGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAtt                                                            <  1:1836299/148‑1 (MQ=255)
                                                                                         tcgGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTtga                                                         <  1:1072119/148‑1 (MQ=255)
                                                                                         tcgGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTtga                                                         >  1:2899611/1‑148 (MQ=255)
                                                                                          cgGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCACTCAAATTtgag                                                        >  2:2845326/1‑147 (MQ=255)
                                                                                          cgGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTtgat                                                        >  2:2844290/1‑148 (MQ=255)
                                                                                            gTTCAAATCCGTCCCCCGCAACCAATTACAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTtgatga                                                      <  1:3223799/148‑1 (MQ=255)
                                                                                             ttCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGTGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCCAAGGTCGTAGGGTAAAATCAGGCCCCCGCAACCAATCAAATTtgatgag                                                     >  1:1927042/1‑147 (MQ=17)
                                                                                             ttCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCACATTTCAGGaa                                                     >  2:2921111/1‑148 (MQ=255)
                                                                                             ttCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTtgatcca                                                     >  2:2920480/1‑145 (MQ=255)
                                                                                             ttCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCGCATAACCCGAAGGTCGTCGGTTCCAATCCGGCCCCCGCAACCAAGCAAAGTtgaggca                                                     >  2:1549439/1‑144 (MQ=14)
                                                                                                aaaTCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTa                                                  >  1:3437358/1‑148 (MQ=255)
                                                                                                aaaTCCGGCCCCCGCAACCAATTAAAATTGGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGCGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCACATCCGGCCCCCGCCACCAATCAGATTtgaggcagga                                                  >  2:2708654/1‑141 (MQ=14)
                                                                                                 aaTCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTaa                                                 <  2:3314306/148‑1 (MQ=255)
                                                                                                    ccgtcacccGCAACCAATTAAAATTTGATG‑AAGTAATGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAg                                              <  2:2133271/142‑1 (MQ=255)
                                                                                                    cccgcacccGCAAACAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAg                                              <  2:768818/142‑1 (MQ=255)
                                                                                                    ccGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGAGCAAGTaactg                                              >  2:561124/1‑145 (MQ=255)
                                                                                                       gCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAg                                           <  2:506384/148‑1 (MQ=255)
                                                                                                        cccccGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTTACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGt                                          >  1:667235/1‑148 (MQ=255)
                                                                                                         ccccGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTa                                         >  1:922648/1‑148 (MQ=255)
                                                                                                            cGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACgg                                      <  1:2573193/148‑1 (MQ=255)
                                                                                                                aCCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGAGGAAGTAAAAGCAGTACGGTGAc                                  >  2:196193/1‑148 (MQ=255)
                                                                                                                  cAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGAcgc                                >  2:1527535/1‑148 (MQ=255)
                                                                                                                    aTTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCgg                              >  1:2064095/1‑148 (MQ=255)
                                                                                                                    aTTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCgg                              <  2:1180746/148‑1 (MQ=255)
                                                                                                                    aTTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGAGGAAGTCCAGGCTGTACGGTGACGCgg                              >  2:2002327/1‑148 (MQ=17)
                                                                                                                      tAAATTTTGATG‑AATTAAAGCATTACTGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCgggg                            <  2:115558/148‑1 (MQ=255)
                                                                                                                      tAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCgggg                            <  1:2016504/148‑1 (MQ=255)
                                                                                                                        aaaTTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCgg                                                                                           >  1:728354/1‑83 (MQ=37)
                                                                                                                        aaaTTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCgg                                                                                           <  2:728354/83‑1 (MQ=37)
                                                                                                                        aaaTTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTg                          >  1:728285/1‑148 (MQ=255)
                                                                                                                        aaaTTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTCATGAAGTAAAAGCAGTACGGTGACGCGGGGTg                          >  2:358796/1‑148 (MQ=255)
                                                                                                                         aaTTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTgg                         <  1:2924693/148‑1 (MQ=255)
                                                                                                                         aaTTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTCAACAATAAAAAGCAGTAAGGTTACGCGGGGTgc                         >  2:1127372/1‑147 (MQ=17)
                                                                                                                           tttGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGag                       >  1:2409847/1‑148 (MQ=255)
                                                                                                                                      tAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGAGGAAGTCCAAGCAGTAAGGTGACGCGGGGTGGAGCAGCCTggaa             >  2:740231/1‑146 (MQ=255)
                                                                                                                                       aaaGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAg            >  2:1579574/1‑148 (MQ=255)
                                                                                                                                        aaccAGTACGGTTACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGc           <  2:336130/145‑1 (MQ=255)
                                                                                                                                         cgCAGTCCGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCt          <  1:2687154/147‑1 (MQ=255)
                                                                                                                                         aGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGCGCAGCCTGGTAGCt          >  1:1711147/1‑148 (MQ=255)
                                                                                                                                          gCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAGTACAGGAAGTAGCTGCTGTACTGGGACGCGGGGTGGAGAAGCCTGGTCGCtc         >  2:738881/1‑148 (MQ=14)
                                                                                                                                              tACGGTTACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATACGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAATCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtcttcg     >  1:1823811/1‑148 (MQ=255)
                                                                                                                                                cgctGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAACAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCggg   <  2:2094488/145‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATG‑AAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGC  >  CP009273/2940707‑2940998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑