Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,941,341 G→A 100% L343L (CTC→CTT)  amiC ← N‑acetylmuramoyl‑L‑alanine amidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,941,3410GA97.9% 621.5 / 2.4 189L343L (CTC→CTT) amiCN‑acetylmuramoyl‑L‑alanine amidase
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base A (88/97);  total (90/99)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 189 total aligned reads displayed.

GAAAGTCGCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCGAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGTTTG  >  CP009273/2941197‑2941481
                                                                                                                                                |                                                                                                                                            
   aGTCGCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTa                                                                                                                                        <  2:2071196/148‑1 (MQ=255)
      cGCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTc                                                                                                                                     <  2:1506249/148‑1 (MQ=255)
        ccGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAg                                                                                                                                   <  2:3404974/148‑1 (MQ=255)
         cGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGt                                                                                                                                  <  2:268162/148‑1 (MQ=255)
          gTTTTCAGTTTACGCTCGTACTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTa                                                                                                                                 <  2:1913194/148‑1 (MQ=255)
              tCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGc                                                                                                                             <  2:1770444/148‑1 (MQ=255)
              tCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGc                                                                                                                             <  1:625471/148‑1 (MQ=255)
               cAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCt                                                                                                                            <  2:984805/148‑1 (MQ=255)
               cAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCt                                                                                                                            <  1:2597733/148‑1 (MQ=255)
               cAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCt                                                                                                                            <  2:3452537/148‑1 (MQ=255)
               cAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCt                                                                                                                            <  1:1565346/148‑1 (MQ=255)
                aGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCtt                                                                                                                           <  2:3087325/148‑1 (MQ=255)
                aGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCtt                                                                                                                           <  2:3089308/148‑1 (MQ=255)
                aGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCtt                                                                                                                           <  2:408223/148‑1 (MQ=255)
                aGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCtt                                                                                                                           <  2:2447265/148‑1 (MQ=255)
                   ttACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTAc                                                                                                                        >  1:3157628/1‑148 (MQ=255)
                   ttACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCGGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTAc                                                                                                                        <  1:3504441/148‑1 (MQ=255)
                          ctTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACtt                                                                                                                 >  2:3071273/1‑148 (MQ=255)
                          ctTCCTCAACGTTACTGATAAACGCCGTATCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACACGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATACAGTACCGCTTTACCAAAcat                                                                                                                 >  1:3359359/1‑146 (MQ=255)
                            tCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCa                                                                                                               >  2:181386/1‑148 (MQ=255)
                             ccTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAg                                                                                                              >  1:3262534/1‑148 (MQ=255)
                             ccTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAg                                                                                                              <  2:821852/148‑1 (MQ=255)
                                cAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCt                                                                                                           <  2:1210632/148‑1 (MQ=255)
                                cAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCt                                                                                                           <  1:646851/148‑1 (MQ=255)
                                     ttACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCgg                                                                                                      >  1:814922/1‑148 (MQ=255)
                                     ttACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCgg                                                                                                      >  1:2218767/1‑148 (MQ=255)
                                              aaCGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCa                                                                                             <  1:72527/148‑1 (MQ=255)
                                                cGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCGAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGc                                                                                           >  2:1026947/1‑148 (MQ=255)
                                                  ccGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGa                                                                                         <  1:483098/148‑1 (MQ=255)
                                                  ccGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGa                                                                                         <  1:2357616/148‑1 (MQ=255)
                                                   cGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGAc                                                                                        <  2:1988042/148‑1 (MQ=255)
                                                      ttCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGt                                                                                     <  2:108782/148‑1 (MQ=255)
                                                           ccATAATGGAGGGAATATCTGGTGCATTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCat                                                                                <  2:911853/148‑1 (MQ=255)
                                                           ccAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCat                                                                                >  1:2445517/1‑148 (MQ=255)
                                                              gAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATc                                                                             >  1:2409627/1‑148 (MQ=255)
                                                               aaTGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCg                                                                            >  1:1027920/1‑148 (MQ=255)
                                                               aaTGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCg                                                                            >  1:3057802/1‑148 (MQ=255)
                                                               aaTGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCg                                                                            >  1:2073782/1‑148 (MQ=255)
                                                               aaTGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCg                                                                            >  1:3234636/1‑148 (MQ=255)
                                                                 tGGAGGGACTATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGTCAATGGTCAGCGACTGTACCATATCGaa                                                                          >  1:441148/1‑148 (MQ=255)
                                                                 tGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGaa                                                                          <  2:2822377/148‑1 (MQ=255)
                                                                  ggAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAAc                                                                         >  1:3185110/1‑148 (MQ=255)
                                                                   gAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACa                                                                        >  1:2855413/1‑148 (MQ=255)
                                                                   gAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACa                                                                        >  1:2858779/1‑148 (MQ=255)
                                                                     ggtaaTATCTGGTGCGTTTAGTACGGAAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATg                                                                      <  1:2993349/145‑1 (MQ=255)
                                                                     ggtaaTATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTTATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTGCCATATCGAACATg                                                                      <  1:1579767/145‑1 (MQ=255)
                                                                     gggAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATg                                                                      >  2:2036213/1‑148 (MQ=255)
                                                                     gggAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATg                                                                      >  2:2122569/1‑148 (MQ=255)
                                                                      ggAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATgg                                                                     >  2:2186128/1‑148 (MQ=255)
                                                                        aaTATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGgtg                                                                   <  1:762835/148‑1 (MQ=255)
                                                                        aaTATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGgtg                                                                   <  1:861138/148‑1 (MQ=255)
                                                                        aaTATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGgtg                                                                   <  1:2641091/148‑1 (MQ=255)
                                                                          tatCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGtgtg                                                                 <  2:112092/148‑1 (MQ=255)
                                                                           atCTGGTGCCTTTAGTCCGGCAACCCCGGCCTGTTCAACTTGATTTTTATGCCGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTgg                                                                <  1:725058/148‑1 (MQ=255)
                                                                           atCTGGTGCATTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTgg                                                                <  1:724469/148‑1 (MQ=255)
                                                                            tCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGt                                                               >  2:3180769/1‑148 (MQ=255)
                                                                              tGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCg                                                             <  2:2770186/148‑1 (MQ=255)
                                                                                gTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGAc                                                           >  1:2749168/1‑148 (MQ=255)
                                                                                gTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGAc                                                           >  2:1567961/1‑148 (MQ=255)
                                                                                 tGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCGAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACa                                                          <  1:3158768/148‑1 (MQ=255)
                                                                                 tGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACa                                                          >  1:400892/1‑148 (MQ=255)
                                                                                  gCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACAt                                                         >  2:2777043/1‑148 (MQ=255)
                                                                                  gCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACAt                                                         <  1:1470812/148‑1 (MQ=255)
                                                                                   ccTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATa                                                        >  2:2320602/1‑148 (MQ=255)
                                                                                      ttAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCg                                                     >  2:2787223/1‑148 (MQ=255)
                                                                                      ttAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCg                                                     >  2:1310399/1‑148 (MQ=255)
                                                                                        aGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGt                                                   <  1:2634329/148‑1 (MQ=255)
                                                                                             ggCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCg                                              >  2:1426564/1‑148 (MQ=255)
                                                                                              gCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGc                                             <  1:2267332/148‑1 (MQ=255)
                                                                                                aaaCCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCtt                                           <  2:2820813/148‑1 (MQ=255)
                                                                                                   cccGGCCTTTTCAACTTGATTTTTATGCAGCTTGTTTATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTg                                        <  1:2753004/148‑1 (MQ=255)
                                                                                                   cccGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTg                                        <  1:1490908/148‑1 (MQ=255)
                                                                                                   cccGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTg                                        <  1:976918/148‑1 (MQ=255)
                                                                                                      ggCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTc                                     <  2:851344/148‑1 (MQ=255)
                                                                                                       gCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCa                                    >  1:2009110/1‑148 (MQ=255)
                                                                                                          tGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGc                                 >  1:1843193/1‑148 (MQ=255)
                                                                                                          tGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGc                                 >  1:3208114/1‑148 (MQ=255)
                                                                                                          tGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACAAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAAAGTTCAGCGAATGTACCATATCGAACATGGTGTGGTCGAAATAGCGGTCACCGCTTTTGCTCAAGc                                 >  1:1843170/1‑148 (MQ=255)
                                                                                                                    gATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCaa                       >  1:1619179/1‑148 (MQ=255)
                                                                                                                    gATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCaa                       >  2:1587509/1‑148 (MQ=255)
                                                                                                                     aTTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAg                      >  2:1045634/1‑148 (MQ=255)
                                                                                                                      tttttATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGt                     <  2:2650951/148‑1 (MQ=255)
                                                                                                                      tttttATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGt                     >  1:3020234/1‑148 (MQ=255)
                                                                                                                       ttttATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTc                    >  1:2470864/1‑148 (MQ=255)
                                                                                                                         ttATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCg                  <  2:2034539/148‑1 (MQ=255)
                                                                                                                         ttATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCg                  <  2:533642/148‑1 (MQ=255)
                                                                                                                           aTGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAg                >  1:1064499/1‑148 (MQ=255)
                                                                                                                            tgctgcTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAgg               <  1:2673448/148‑1 (MQ=255)
                                                                                                                             gCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGc              >  2:2920540/1‑148 (MQ=255)
                                                                                                                                gCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGtt           <  1:261317/148‑1 (MQ=255)
                                                                                                                                 cTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTc          >  2:722816/1‑148 (MQ=255)
                                                                                                                                 cTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTc          >  2:1637584/1‑148 (MQ=255)
                                                                                                                                 cTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTACAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTc          <  1:531657/148‑1 (MQ=255)
                                                                                                                                  ttgttgATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCt         >  1:1792646/1‑148 (MQ=255)
                                                                                                                                  ttgttgATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCt         >  1:1791318/1‑148 (MQ=255)
                                                                                                                                   tgttgATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTg        >  2:1860458/1‑148 (MQ=255)
                                                                                                                                    gttgATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTgg       <  2:3445439/148‑1 (MQ=255)
                                                                                                                                     ttgATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTggg      >  1:3461695/1‑148 (MQ=255)
                                                                                                                                        aTTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGttt   >  1:1831401/1‑148 (MQ=255)
                                                                                                                                         ttttACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGTTtg  <  2:1964534/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                            
GAAAGTCGCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCGAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGTTTG  >  CP009273/2941197‑2941481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑