Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,983,568 C→A 19.1% R51I (AGA→ATA)  yqeK ← uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,983,5680CA19.1% 804.3 / 122.9 297R51I (AGA→ATA) yqeKuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (109/131);  new base A (26/31);  total (135/162)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 301 total aligned reads displayed.

TCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGT  >  CP009273/2983421‑2983715
                                                                                                                                                   |                                                                                                                                                   
    tAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGa                                                                                                                                                 >  1:1996872/1‑148 (MQ=255)
    tAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGa                                                                                                                                                 >  1:615427/1‑148 (MQ=255)
    tAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCAACTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGa                                                                                                                                                 >  1:1860698/1‑148 (MQ=255)
     agagGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGaa                                                                                                                                                <  1:1787395/148‑1 (MQ=255)
     agagGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGaa                                                                                                                                                <  1:1121962/148‑1 (MQ=255)
      gagGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAAt                                                                                                                                               >  2:1363601/1‑148 (MQ=255)
       agGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGAAtt                                                                                                                                              >  2:2844321/1‑148 (MQ=255)
        ggAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAAttt                                                                                                                                             <  1:1893556/148‑1 (MQ=255)
          aaaGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTaa                                                                                                                                           <  2:1294792/148‑1 (MQ=255)
           aaGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAAt                                                                                                                                          <  1:713641/148‑1 (MQ=255)
             ggACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGt                                                                                                                                        >  1:449792/1‑148 (MQ=255)
             ggACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGt                                                                                                                                        <  1:936884/148‑1 (MQ=255)
                cTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCa                                                                                                                                     <  1:345310/148‑1 (MQ=255)
                  aaCGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCatg                                                                                                                                   <  2:1769911/148‑1 (MQ=255)
                   aCGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCatga                                                                                                                                  <  1:1390877/148‑1 (MQ=255)
                   aCGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCatga                                                                                                                                  >  2:1201258/1‑148 (MQ=255)
                     gTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCatgatg                                                                                                                                <  1:3395015/148‑1 (MQ=255)
                       ttCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGAtgtt                                                                                                                              >  1:2093085/1‑148 (MQ=255)
                         cTTTAAAAGAATTGATTTTATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGAtgttgt                                                                                                                            <  2:3092833/148‑1 (MQ=255)
                         cTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGAtgttgt                                                                                                                            >  2:3017730/1‑148 (MQ=255)
                          tttAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATgttgtt                                                                                                                           <  1:841934/148‑1 (MQ=255)
                          tttAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATgttgtt                                                                                                                           <  2:3169158/148‑1 (MQ=255)
                          tttAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATgttgtt                                                                                                                           >  1:3245266/1‑148 (MQ=255)
                             aaaaGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGc                                                                                                                        <  1:3101285/148‑1 (MQ=255)
                                aGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTg                                                                                                                     <  2:2208992/148‑1 (MQ=255)
                                   aTTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTgaga                                                                                                                  >  1:2520769/1‑148 (MQ=255)
                                   aTTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTgaga                                                                                                                  >  2:2345524/1‑148 (MQ=255)
                                    ttGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGTGTGAGaa                                                                                                                 >  2:2415937/1‑148 (MQ=255)
                                     tGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAAc                                                                                                                <  1:270995/148‑1 (MQ=255)
                                      gATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACa                                                                                                               <  1:3497872/148‑1 (MQ=255)
                                        tttCATCATCTGTAAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACAtt                                                                                                             <  2:2305772/148‑1 (MQ=255)
                                           catcatCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATtctc                                                                                                          >  2:1425129/1‑148 (MQ=255)
                                             tcatcTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAg                                                                                                        >  1:2872046/1‑148 (MQ=255)
                                               atcTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCa                                                                                                      >  1:1007267/1‑148 (MQ=255)
                                                   gTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTaa                                                                                                  <  1:2027002/148‑1 (MQ=255)
                                                    ttAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAg                                                                                                 <  1:396721/148‑1 (MQ=255)
                                                     taaactaaacTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAgg                                                                                                <  2:1871427/148‑1 (MQ=255)
                                                          taaactCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAAttt                                                                                           <  2:1612290/148‑1 (MQ=255)
                                                              ctCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAAttttttt                                                                                       <  1:2629094/148‑1 (MQ=255)
                                                               tcatcaTTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTa                                                                                      <  2:449746/148‑1 (MQ=255)
                                                                catcatTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTaa                                                                                     <  1:949913/148‑1 (MQ=255)
                                                                  tcatTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACg                                                                                   >  1:859404/1‑148 (MQ=255)
                                                                   catTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACgg                                                                                  <  1:3043737/148‑1 (MQ=255)
                                                                    atTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGa                                                                                 <  2:512768/148‑1 (MQ=255)
                                                                      tGACAGATCGTTAGATATAACTGTTTTTATCTTTACTGTTTACGTTGACTTTATTGACAGAATTAACATTCAAATTTATTGAATTTTATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAAt                                                                               <  1:3441784/148‑1 (MQ=255)
                                                                      tGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGTAAt                                                                               >  1:553751/1‑148 (MQ=255)
                                                                      tGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAAt                                                                               >  2:3308840/1‑148 (MQ=255)
                                                                      tGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAAt                                                                               <  1:1609712/148‑1 (MQ=255)
                                                                          aGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCtt                                                                           <  2:2574990/148‑1 (MQ=255)
                                                                           gATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTg                                                                          <  2:3349753/148‑1 (MQ=255)
                                                                            aTCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTgg                                                                         <  2:1712367/148‑1 (MQ=255)
                                                                             tCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGt                                                                        <  2:2871775/148‑1 (MQ=255)
                                                                              cGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGtt                                                                       <  2:641275/148‑1 (MQ=255)
                                                                                tGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTc                                                                     >  2:2085703/1‑148 (MQ=255)
                                                                                  agaTATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCtt                                                                   >  2:1218312/1‑148 (MQ=255)
                                                                                     tataACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCttttt                                                                >  1:1924684/1‑148 (MQ=255)
                                                                                     tataACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCttttt                                                                <  2:1561612/148‑1 (MQ=255)
                                                                                      ataACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTc                                                               >  2:2933226/1‑148 (MQ=255)
                                                                                       taACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAAAATTCACATATCATGAAATTAATGTCCATGATGTTGTTTGCGTGAGAACATACTCAGCATTAAGGAATTTTTTTAAAGGAATCCTTGGTTTCTTTTTCg                                                              >  1:3143013/1‑148 (MQ=255)
                                                                                        aaCTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGa                                                             >  2:2468370/1‑148 (MQ=255)
                                                                                        aaCTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGa                                                             >  2:2768351/1‑148 (MQ=255)
                                                                                          cTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGt                                                           <  2:776840/148‑1 (MQ=255)
                                                                                              ttttAACTTTAATCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATTTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGa                                                       <  2:3502839/148‑1 (MQ=255)
                                                                                               tttAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGaa                                                      <  2:615427/148‑1 (MQ=255)
                                                                                               tttAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGaa                                                      <  2:2400843/148‑1 (MQ=255)
                                                                                               tttAACTTTACTATTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATTATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTTGTTTCTTTTTCGAGTCCGaa                                                      <  2:1847283/148‑1 (MQ=255)
                                                                                                  aaCTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAAttt                                                   >  2:2020312/1‑148 (MQ=255)
                                                                                                     tttACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACa                                                <  2:2260431/148‑1 (MQ=255)
                                                                                                       tACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAat                                              >  1:1178534/1‑148 (MQ=255)
                                                                                                        aCTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAata                                             <  1:3220486/148‑1 (MQ=255)
                                                                                                           ctTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCa                                          <  2:2819757/148‑1 (MQ=255)
                                                                                                            tttACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCAt                                         >  1:606150/1‑148 (MQ=255)
                                                                                                              tACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGc                                       <  1:1474344/148‑1 (MQ=255)
                                                                                                               aCGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCa                                      <  2:2325125/148‑1 (MQ=255)
                                                                                                                cGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCat                                     >  2:3225942/1‑148 (MQ=255)
                                                                                                                cGTTGACTTTATTGACAGAATTAACATTCACATATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCat                                     <  2:1907118/148‑1 (MQ=255)
                                                                                                                  ttGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCatat                                   >  2:477903/1‑148 (MQ=255)
                                                                                                                       tttATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGAcc                              <  1:1051834/148‑1 (MQ=255)
                                                                                                                          aTTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCAtt                           <  2:678443/148‑1 (MQ=255)
                                                                                                                          aTTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCAtt                           <  1:1025794/148‑1 (MQ=255)
                                                                                                                            tGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTc                         <  1:500966/148‑1 (MQ=255)
                                                                                                                             gACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCa                        <  1:2326278/148‑1 (MQ=255)
                                                                                                                             gACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCa                        <  2:2520769/148‑1 (MQ=255)
                                                                                                                              aCAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCAt                       <  1:2634906/148‑1 (MQ=255)
                                                                                                                               cAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATg                      >  2:1122236/1‑148 (MQ=255)
                                                                                                                                aGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGa                     >  2:1878645/1‑148 (MQ=255)
                                                                                                                                  aaTTAACATTCACATATCTTGAATTTAATGTCCATGATGGTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAAt                   >  1:2880529/1‑148 (MQ=255)
                                                                                                                                   aTTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATc                  <  2:3436921/148‑1 (MQ=255)
                                                                                                                                     tAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTg                >  1:236265/1‑148 (MQ=255)
                                                                                                                                     tAACATTCACATATATTGAATTTAATGTCCATGAGGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTg                >  1:3315973/1‑148 (MQ=255)
                                                                                                                                      aaCATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGc               <  1:646082/148‑1 (MQ=255)
                                                                                                                                       aCATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCg              >  1:1215511/1‑148 (MQ=255)
                                                                                                                                        cATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGa             >  2:721880/1‑148 (MQ=255)
                                                                                                                                         aTTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGaa            <  1:2414815/148‑1 (MQ=255)
                                                                                                                                          ttCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGaaa           >  2:925862/1‑148 (MQ=255)
                                                                                                                                             acaTATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAAtt        <  2:1386023/148‑1 (MQ=255)
                                                                                                                                             acaTATATTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAAtt        <  1:788232/148‑1 (MQ=255)
                                                                                                                                               atatCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCa      >  1:2977274/1‑148 (MQ=255)
                                                                                                                                                tatCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCaa     <  1:1363601/148‑1 (MQ=255)
                                                                                                                                                  tCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATg   >  1:2194782/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TCAATAGAGGAAAGGACTAACGTTTCTTTAAAAGAATTGATTTCATCATCTGTTAAACTAAACTCATCATTGACAGATCGTGAGATATAACTGTTTTTAACTTTACTCTTTACGTTGACTTTATTGACAGAATTAACATTCACATATCTTGAATTTAATGTCCATGATGTTGTTTGCGTGAGAACATTCTCAGCATTAAGGAATTTTTTTAACGGAATCCTTGGTTTCTTTTTCGAGTCCGAATTTACAATATCATGCATATAGACCATTTCATGAATCTGCGAAAATTCAATGT  >  CP009273/2983421‑2983715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑