Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,018,544 G→T 100% A279S (GCC→TCC)  xanQ → xanthine permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,018,5440GT96.3% 668.2 / 14.6 217A279S (GCC→TCC) xanQxanthine permease
Reads supporting (aligned to +/- strand):  ref base G (4/4);  new base T (108/101);  total (112/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 217 total aligned reads displayed.

GGTGGATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCAAAATAATG  >  CP009273/3018399‑3018688
                                                                                                                                                 |                                                                                                                                                
ggtggATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTcc                                                                                                                                                <  2:327328/148‑1 (MQ=255)
  tggATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCAc                                                                                                                                              >  1:3060350/1‑148 (MQ=255)
   ggATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACg                                                                                                                                             >  1:2607047/1‑148 (MQ=255)
     aTTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGc                                                                                                                                           >  2:2151447/1‑148 (MQ=255)
      tttCAGCAGTATGCGCAATTTGCAGTTAATCACCATCCCGCATCCGTTCAAATACGGCATTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCa                                                                                                                                          >  1:1128367/1‑148 (MQ=255)
       ttCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCaa                                                                                                                                         >  1:3031242/1‑148 (MQ=255)
       ttCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCaa                                                                                                                                         >  1:1896618/1‑148 (MQ=255)
       ttCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCaa                                                                                                                                         <  2:1664099/148‑1 (MQ=255)
        tCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAAt                                                                                                                                        >  2:2579564/1‑148 (MQ=255)
          agcagTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATgg                                                                                                                                      <  2:499473/148‑1 (MQ=255)
             agTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGttt                                                                                                                                   <  2:889568/148‑1 (MQ=255)
             agTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGttt                                                                                                                                   <  2:847031/148‑1 (MQ=255)
              gTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTc                                                                                                                                  <  2:1432952/148‑1 (MQ=255)
               tATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTcc                                                                                                                                 >  1:1236731/1‑148 (MQ=255)
               tATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTcc                                                                                                                                 >  1:1057738/1‑148 (MQ=255)
               tATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGAGTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTcc                                                                                                                                 >  1:3297936/1‑148 (MQ=255)
                     cactttGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCCcgccgc                                                                                                                           <  2:2210846/145‑1 (MQ=255)
                         ttGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGATGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCCCGCCGCCCCa                                                                                                                       >  2:2075181/1‑148 (MQ=255)
                            ccGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTc                                                                                                                    >  1:2456398/1‑148 (MQ=255)
                                   tCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGa                                                                                                             >  1:3188580/1‑148 (MQ=255)
                                   tCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGa                                                                                                             <  2:2814489/148‑1 (MQ=255)
                                   tCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGa                                                                                                             <  1:1138419/148‑1 (MQ=255)
                                        aTCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGt                                                                                                        >  2:3430531/1‑148 (MQ=255)
                                         tCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTa                                                                                                       <  1:2821737/148‑1 (MQ=255)
                                         tCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTa                                                                                                       <  1:3122032/148‑1 (MQ=255)
                                         tCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTa                                                                                                       <  1:2769769/148‑1 (MQ=255)
                                           ccGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATc                                                                                                     <  2:746395/148‑1 (MQ=255)
                                              cATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGt                                                                                                  <  1:2340900/148‑1 (MQ=255)
                                              cATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGt                                                                                                  <  2:2353721/148‑1 (MQ=255)
                                                tCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTcc                                                                                                <  1:3145389/148‑1 (MQ=255)
                                                     tCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCt                                                                                           >  2:163219/1‑148 (MQ=255)
                                                     tCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCt                                                                                           <  1:3515521/148‑1 (MQ=255)
                                                       aaaTACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGa                                                                                         >  2:3398846/1‑148 (MQ=255)
                                                       aaaTACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGa                                                                                         >  2:1637011/1‑148 (MQ=255)
                                                          tACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAg                                                                                      <  1:3494900/148‑1 (MQ=255)
                                                              gCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAggcgg                                                                                  <  2:3128155/148‑1 (MQ=255)
                                                               cTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAggcggc                                                                                 >  2:688799/1‑148 (MQ=255)
                                                               cTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAggcggc                                                                                 >  1:1475762/1‑148 (MQ=255)
                                                                  tAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTg                                                                              <  2:1029644/148‑1 (MQ=255)
                                                                     ttttAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTg                                                                           >  2:3207464/1‑148 (MQ=255)
                                                                     ttttAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTg                                                                           >  1:976924/1‑148 (MQ=255)
                                                                     ttttAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTg                                                                           >  1:3487480/1‑148 (MQ=255)
                                                                         aGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAg                                                                       <  2:2707295/148‑1 (MQ=255)
                                                                          gCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGa                                                                      <  2:3089902/148‑1 (MQ=255)
                                                                           cTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGAt                                                                     >  1:1218955/1‑148 (MQ=255)
                                                                            ttCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATg                                                                    >  2:185346/1‑148 (MQ=255)
                                                                            ttCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATg                                                                    >  2:1794835/1‑148 (MQ=255)
                                                                              ccATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGt                                                                  <  2:3370363/148‑1 (MQ=255)
                                                                                aTCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCt                                                                >  2:1386028/1‑148 (MQ=255)
                                                                                  cAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTgg                                                              <  2:2365099/148‑1 (MQ=255)
                                                                                   aGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGt                                                             <  2:472188/148‑1 (MQ=255)
                                                                                    gTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGtt                                                            >  1:510703/1‑148 (MQ=255)
                                                                                     ttCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGttt                                                           <  2:670278/148‑1 (MQ=255)
                                                                                     ttCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGttt                                                           <  2:2269453/148‑1 (MQ=255)
                                                                                      tCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTc                                                          <  1:2970749/148‑1 (MQ=255)
                                                                                       ccTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCt                                                         <  1:2641608/148‑1 (MQ=255)
                                                                                        cTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTg                                                        <  2:1944665/148‑1 (MQ=255)
                                                                                          ggtggtTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGtt                                                      <  2:295316/148‑1 (MQ=255)
                                                                                          ggtggtTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGtt                                                      <  2:3309060/148‑1 (MQ=255)
                                                                                           gtggtTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTa                                                     <  2:961497/148‑1 (MQ=255)
                                                                                              gtTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCg                                                  >  2:387192/1‑148 (MQ=255)
                                                                                               ttGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGc                                                 <  2:1131611/148‑1 (MQ=255)
                                                                                                 ggCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCt                                               <  1:2065754/148‑1 (MQ=255)
                                                                                                  gCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTc                                              <  2:1897265/148‑1 (MQ=255)
                                                                                                  gCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTc                                              >  2:1811684/1‑148 (MQ=255)
                                                                                                  gCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTc                                              >  2:3467937/1‑148 (MQ=255)
                                                                                                  gCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTc                                              >  2:1814716/1‑148 (MQ=255)
                                                                                                    aCGATTTATCTGCTTAGCGTGATGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCg                                            <  1:2075181/148‑1 (MQ=255)
                                                                                                       aTTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTg                                         <  2:1716167/148‑1 (MQ=255)
                                                                                                        tttATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGt                                        <  1:1373885/148‑1 (MQ=255)
                                                                                                        tttATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGt                                        >  1:1977324/1‑148 (MQ=255)
                                                                                                           aTCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCgg                                     >  1:832610/1‑148 (MQ=255)
                                                                                                            tCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGt                                    <  2:980368/148‑1 (MQ=255)
                                                                                                            tCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGt                                    <  1:668699/148‑1 (MQ=255)
                                                                                                                cTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCAt                                >  1:2874792/1‑148 (MQ=255)
                                                                                                                cTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCAt                                <  2:1624647/148‑1 (MQ=255)
                                                                                                                 ttAGCGTGCTGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCAtt                               >  2:2169513/1‑148 (MQ=255)
                                                                                                                  tAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTa                              <  1:1766909/148‑1 (MQ=255)
                                                                                                                  tAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTa                              >  2:1823459/1‑148 (MQ=255)
                                                                                                                   aGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTAc                             >  2:1281213/1‑148 (MQ=255)
                                                                                                                    gCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTAcc                            <  1:1936733/148‑1 (MQ=255)
                                                                                                                     cGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCa                           >  2:2383532/1‑148 (MQ=255)
                                                                                                                        gCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTa                        <  2:3060350/148‑1 (MQ=255)
                                                                                                                        gCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTa                        <  1:1650257/148‑1 (MQ=255)
                                                                                                                         cTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTaa                       <  1:2208158/148‑1 (MQ=255)
                                                                                                                           ggAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAAcc                     >  1:1321380/1‑148 (MQ=255)
                                                                                                                             aaGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCAc                   >  2:855086/1‑148 (MQ=255)
                                                                                                                             aaGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCAc                   >  1:1745610/1‑148 (MQ=255)
                                                                                                                               gCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGt                 >  2:2884482/1‑148 (MQ=255)
                                                                                                                                cAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGtt                >  1:867143/1‑148 (MQ=255)
                                                                                                                                    cGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTgcg            <  2:2872181/148‑1 (MQ=255)
                                                                                                                                    cGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTgcg            <  2:2531918/148‑1 (MQ=255)
                                                                                                                                      gCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGGTTGCGCa          >  2:1770072/1‑148 (MQ=255)
                                                                                                                                      gCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGATTTCGCa          >  2:2738195/1‑148 (MQ=255)
                                                                                                                                         atatCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCaaaa       <  2:3005666/148‑1 (MQ=255)
                                                                                                                                          tatCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGTGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCAAaat      >  1:2296760/1‑148 (MQ=255)
                                                                                                                                            tCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCAAaataa    <  1:2178276/148‑1 (MQ=255)
                                                                                                                                            tCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCAAaataa    >  1:267049/1‑148 (MQ=255)
                                                                                                                                              aCCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCAAAATAATg  <  2:2879895/148‑1 (MQ=255)
                                                                                                                                              aCCGCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCAAAATAATg  >  1:3511494/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGTGGATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCAAAATAATG  >  CP009273/3018399‑3018688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑